==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER VIRAL PROTEIN 01-JUN-05 1ZVB . COMPND 2 MOLECULE: E2 GLYCOPROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: SARS CORONAVIRUS; . AUTHOR Y.DENG,J.LIU,Q.ZHENG,W.YONG,J.DAI,M.LU . 101 3 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 6556.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 90 89.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 3 3.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 87 86.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A A > 0 0 115 0, 0.0 4,-2.2 0, 0.0 5,-0.1 0.000 360.0 360.0 360.0 161.7 13.4 9.8 1.7 2 2 A L H > + 0 0 95 1,-0.2 4,-2.5 2,-0.2 5,-0.2 0.925 360.0 51.4 -62.4 -47.4 12.7 6.2 2.8 3 3 A N H > S+ 0 0 94 1,-0.2 4,-2.2 2,-0.2 -1,-0.2 0.919 110.5 50.7 -60.0 -40.5 16.2 5.4 3.9 4 4 A T H > S+ 0 0 67 2,-0.2 4,-2.5 1,-0.2 -1,-0.2 0.928 109.2 51.3 -59.8 -45.1 16.3 8.6 6.1 5 5 A L H X S+ 0 0 19 -4,-2.2 4,-2.9 1,-0.2 -2,-0.2 0.917 108.6 50.9 -58.4 -47.0 13.0 7.6 7.7 6 6 A V H X S+ 0 0 58 -4,-2.5 4,-2.5 1,-0.2 -1,-0.2 0.916 110.9 48.6 -61.1 -40.4 14.3 4.2 8.5 7 7 A K H X S+ 0 0 134 -4,-2.2 4,-1.9 1,-0.2 -1,-0.2 0.899 111.2 50.1 -65.9 -38.9 17.4 5.7 10.2 8 8 A Q H X S+ 0 0 108 -4,-2.5 4,-2.6 1,-0.2 -2,-0.2 0.894 110.1 51.3 -64.1 -43.1 15.2 8.1 12.2 9 9 A L H X S+ 0 0 8 -4,-2.9 4,-2.7 1,-0.2 5,-0.3 0.932 108.2 50.6 -63.5 -45.0 13.0 5.2 13.3 10 10 A S H X S+ 0 0 79 -4,-2.5 4,-2.1 1,-0.2 -1,-0.2 0.890 111.4 48.8 -58.4 -39.4 16.0 3.2 14.5 11 11 A S H X S+ 0 0 82 -4,-1.9 4,-2.0 1,-0.2 -1,-0.2 0.928 112.1 49.2 -66.9 -43.7 17.3 6.2 16.5 12 12 A N H X S+ 0 0 62 -4,-2.6 4,-2.5 1,-0.2 -2,-0.2 0.912 112.5 45.8 -59.8 -49.2 13.9 6.8 18.0 13 13 A F H X S+ 0 0 32 -4,-2.7 4,-2.7 1,-0.2 -1,-0.2 0.867 111.0 53.9 -66.8 -34.5 13.4 3.2 19.1 14 14 A G H X S+ 0 0 43 -4,-2.1 4,-2.1 -5,-0.3 -1,-0.2 0.885 110.2 46.9 -61.5 -39.4 17.0 3.1 20.5 15 15 A A H X S+ 0 0 54 -4,-2.0 4,-2.8 2,-0.2 -2,-0.2 0.891 111.4 51.5 -72.3 -43.9 16.2 6.2 22.6 16 16 A I H X S+ 0 0 16 -4,-2.5 4,-3.0 1,-0.2 5,-0.2 0.941 109.5 50.2 -59.7 -42.3 12.9 4.6 23.8 17 17 A S H X S+ 0 0 61 -4,-2.7 4,-2.6 1,-0.2 -2,-0.2 0.912 110.6 49.7 -61.3 -43.5 14.7 1.4 24.8 18 18 A S H X S+ 0 0 79 -4,-2.1 4,-2.1 2,-0.2 -1,-0.2 0.925 113.2 45.7 -57.8 -49.1 17.3 3.4 26.7 19 19 A V H X S+ 0 0 50 -4,-2.8 4,-2.7 1,-0.2 -2,-0.2 0.938 113.1 49.9 -65.3 -42.9 14.7 5.4 28.6 20 20 A L H X S+ 0 0 12 -4,-3.0 4,-3.0 1,-0.2 -1,-0.2 0.899 109.8 50.8 -60.7 -41.5 12.6 2.3 29.3 21 21 A N H X S+ 0 0 91 -4,-2.6 4,-2.3 -5,-0.2 -1,-0.2 0.893 110.3 49.4 -65.1 -39.1 15.6 0.4 30.7 22 22 A D H X S+ 0 0 81 -4,-2.1 4,-2.3 2,-0.2 5,-0.2 0.938 113.1 47.4 -64.9 -46.8 16.5 3.4 33.0 23 23 A I H X S+ 0 0 1 -4,-2.7 4,-2.9 1,-0.2 5,-0.2 0.932 111.4 50.5 -57.1 -46.7 12.9 3.5 34.2 24 24 A L H X S+ 0 0 40 -4,-3.0 4,-2.6 1,-0.2 -1,-0.2 0.910 111.5 48.0 -62.1 -41.0 12.7 -0.3 34.8 25 25 A S H X S+ 0 0 67 -4,-2.3 4,-1.7 2,-0.2 -1,-0.2 0.904 114.2 45.9 -66.1 -42.1 16.0 -0.2 36.8 26 26 A R H X S+ 0 0 35 -4,-2.3 4,-2.3 2,-0.2 -2,-0.2 0.877 112.5 50.5 -67.9 -37.7 14.9 2.7 38.9 27 27 A L H X S+ 0 0 0 -4,-2.9 4,-2.8 1,-0.2 5,-0.2 0.918 107.5 54.0 -70.2 -39.5 11.4 1.1 39.5 28 28 A D H X S+ 0 0 87 -4,-2.6 4,-1.5 -5,-0.2 -1,-0.2 0.918 109.6 48.6 -54.9 -46.5 13.1 -2.1 40.5 29 29 A K H < S+ 0 0 115 -4,-1.7 4,-0.5 2,-0.2 -2,-0.2 0.889 112.0 47.3 -65.0 -42.9 15.1 -0.2 43.1 30 30 A V H >X S+ 0 0 2 -4,-2.3 3,-1.6 1,-0.2 4,-0.9 0.930 109.5 53.6 -63.2 -43.9 12.1 1.7 44.6 31 31 A E H 3< S+ 0 0 66 -4,-2.8 3,-0.5 1,-0.3 -1,-0.2 0.835 106.3 54.1 -59.3 -34.5 10.0 -1.5 44.8 32 32 A A T 3< S+ 0 0 84 -4,-1.5 -1,-0.3 -5,-0.2 -2,-0.2 0.590 104.7 55.8 -73.4 -13.5 12.8 -3.1 46.8 33 33 A E T <4 0 0 119 -3,-1.6 -1,-0.2 -4,-0.5 -2,-0.2 0.580 360.0 360.0-101.3 -13.0 12.8 -0.2 49.3 34 34 A V < 0 0 101 -4,-0.9 -2,-0.2 -3,-0.5 -3,-0.1 0.880 360.0 360.0 -78.2 360.0 9.1 -0.4 50.2 35 !* 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 36 2 B L > 0 0 103 0, 0.0 4,-2.3 0, 0.0 5,-0.2 0.000 360.0 360.0 360.0 28.9 4.6 8.2 2.6 37 3 B N H > + 0 0 129 1,-0.2 4,-2.0 2,-0.2 5,-0.1 0.857 360.0 51.0 -57.6 -43.8 2.6 11.1 4.2 38 4 B T H > S+ 0 0 101 2,-0.2 4,-2.2 1,-0.2 -1,-0.2 0.937 111.8 45.7 -64.8 -47.9 0.0 8.9 5.9 39 5 B L H > S+ 0 0 22 1,-0.2 4,-2.8 2,-0.2 -1,-0.2 0.905 112.2 50.9 -62.9 -44.2 2.6 6.6 7.6 40 6 B V H X S+ 0 0 68 -4,-2.3 4,-2.7 2,-0.2 -1,-0.2 0.913 109.6 50.5 -62.5 -36.0 4.7 9.5 8.7 41 7 B K H X S+ 0 0 155 -4,-2.0 4,-2.0 1,-0.2 -1,-0.2 0.915 111.9 48.0 -67.0 -41.7 1.7 11.3 10.3 42 8 B Q H X S+ 0 0 109 -4,-2.2 4,-2.4 2,-0.2 -1,-0.2 0.903 111.9 49.3 -63.1 -43.1 0.8 8.1 12.1 43 9 B L H X S+ 0 0 8 -4,-2.8 4,-2.9 1,-0.2 5,-0.3 0.917 109.0 52.5 -64.1 -45.4 4.4 7.6 13.4 44 10 B S H X S+ 0 0 84 -4,-2.7 4,-2.6 1,-0.2 -1,-0.2 0.913 110.3 48.8 -55.8 -44.7 4.5 11.3 14.6 45 11 B S H X S+ 0 0 82 -4,-2.0 4,-2.2 1,-0.2 -1,-0.2 0.925 112.5 47.7 -60.3 -47.8 1.3 10.7 16.5 46 12 B N H X S+ 0 0 64 -4,-2.4 4,-2.6 2,-0.2 -2,-0.2 0.915 113.0 47.2 -61.0 -45.9 2.6 7.5 18.1 47 13 B F H X S+ 0 0 33 -4,-2.9 4,-2.6 1,-0.2 -1,-0.2 0.886 110.3 53.1 -67.9 -36.7 5.9 9.0 19.1 48 14 B G H X S+ 0 0 44 -4,-2.6 4,-2.2 -5,-0.3 -1,-0.2 0.918 110.4 48.0 -60.1 -43.3 4.2 12.1 20.6 49 15 B A H X S+ 0 0 55 -4,-2.2 4,-2.4 2,-0.2 -2,-0.2 0.910 111.3 50.3 -65.5 -42.9 2.0 9.8 22.7 50 16 B I H X S+ 0 0 15 -4,-2.6 4,-3.2 1,-0.2 5,-0.2 0.937 110.0 49.6 -60.6 -43.7 5.0 7.8 23.9 51 17 B S H X S+ 0 0 62 -4,-2.6 4,-2.6 1,-0.2 -1,-0.2 0.911 111.0 50.7 -62.2 -42.2 7.0 10.9 24.8 52 18 B S H X S+ 0 0 80 -4,-2.2 4,-2.0 2,-0.2 -1,-0.2 0.919 113.6 44.0 -60.5 -47.3 3.9 12.2 26.8 53 19 B V H X S+ 0 0 48 -4,-2.4 4,-2.6 2,-0.2 -2,-0.2 0.926 113.1 51.2 -65.8 -44.1 3.6 8.9 28.7 54 20 B L H X S+ 0 0 14 -4,-3.2 4,-2.9 1,-0.2 5,-0.2 0.921 109.4 51.2 -57.6 -45.4 7.3 8.6 29.3 55 21 B N H X S+ 0 0 96 -4,-2.6 4,-2.3 -5,-0.2 -1,-0.2 0.900 110.3 48.5 -59.4 -44.7 7.4 12.2 30.7 56 22 B D H X S+ 0 0 83 -4,-2.0 4,-2.2 1,-0.2 -1,-0.2 0.914 112.7 48.9 -63.7 -41.5 4.5 11.4 33.1 57 23 B I H X S+ 0 0 0 -4,-2.6 4,-3.0 2,-0.2 -2,-0.2 0.927 110.8 49.7 -61.5 -43.2 6.2 8.2 34.2 58 24 B L H X S+ 0 0 41 -4,-2.9 4,-2.7 1,-0.2 -2,-0.2 0.912 111.2 49.5 -66.6 -38.2 9.5 10.0 34.9 59 25 B S H X S+ 0 0 69 -4,-2.3 4,-1.7 -5,-0.2 -1,-0.2 0.923 113.8 45.1 -63.4 -46.4 7.8 12.7 36.9 60 26 B R H X S+ 0 0 45 -4,-2.2 4,-2.4 2,-0.2 -2,-0.2 0.879 112.7 51.3 -64.7 -40.5 6.0 10.2 39.0 61 27 B L H X S+ 0 0 1 -4,-3.0 4,-2.5 1,-0.2 5,-0.2 0.920 106.0 55.0 -65.6 -42.9 9.1 8.1 39.4 62 28 B D H X S+ 0 0 87 -4,-2.7 4,-1.5 -5,-0.2 -1,-0.2 0.908 109.8 47.7 -54.0 -46.5 11.1 11.1 40.6 63 29 B K H X S+ 0 0 144 -4,-1.7 4,-0.9 1,-0.2 -2,-0.2 0.935 111.3 48.3 -65.4 -45.5 8.4 11.8 43.3 64 30 B V H >X S+ 0 0 1 -4,-2.4 4,-1.3 1,-0.2 3,-0.7 0.885 107.8 55.3 -62.7 -38.5 8.4 8.2 44.6 65 31 B E H 3< S+ 0 0 61 -4,-2.5 3,-0.3 1,-0.3 -1,-0.2 0.885 108.9 49.2 -60.4 -35.1 12.2 8.1 44.7 66 32 B A H 3< S+ 0 0 85 -4,-1.5 -1,-0.3 -5,-0.2 -2,-0.2 0.681 111.6 50.2 -73.0 -19.1 11.9 11.1 46.9 67 33 B E H << 0 0 106 -4,-0.9 -2,-0.2 -3,-0.7 -1,-0.2 0.594 360.0 360.0-100.1 -12.7 9.3 9.5 49.1 68 34 B V < 0 0 90 -4,-1.3 -1,-0.1 -3,-0.3 -2,-0.1 0.780 360.0 360.0 -75.6 360.0 10.9 6.1 49.9 69 !* 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 70 1 C A > 0 0 109 0, 0.0 4,-2.0 0, 0.0 3,-0.4 0.000 360.0 360.0 360.0-161.8 9.1 -3.5 1.2 71 2 C L H > + 0 0 80 1,-0.2 4,-2.7 2,-0.2 5,-0.2 0.881 360.0 58.1 -59.1 -40.7 6.5 -1.3 2.9 72 3 C N H > S+ 0 0 97 1,-0.2 4,-2.0 2,-0.2 -1,-0.2 0.895 106.3 49.2 -58.2 -40.6 4.5 -4.2 4.2 73 4 C T H > S+ 0 0 67 -3,-0.4 4,-2.3 2,-0.2 -1,-0.2 0.923 111.8 49.1 -61.8 -47.7 7.7 -5.5 6.1 74 5 C L H X S+ 0 0 22 -4,-2.0 4,-2.6 1,-0.2 5,-0.2 0.930 110.1 49.7 -61.2 -45.7 8.3 -2.1 7.6 75 6 C V H X S+ 0 0 60 -4,-2.7 4,-2.7 1,-0.2 -1,-0.2 0.890 110.8 50.1 -62.1 -39.6 4.7 -1.7 8.8 76 7 C K H X S+ 0 0 148 -4,-2.0 4,-2.2 -5,-0.2 -1,-0.2 0.905 111.7 48.7 -64.6 -41.4 4.7 -5.2 10.4 77 8 C Q H X S+ 0 0 107 -4,-2.3 4,-2.4 2,-0.2 -2,-0.2 0.899 112.8 47.2 -63.8 -42.3 8.0 -4.3 12.2 78 9 C L H X S+ 0 0 9 -4,-2.6 4,-3.0 2,-0.2 5,-0.3 0.911 110.3 52.7 -65.7 -44.0 6.6 -1.0 13.4 79 10 C S H X S+ 0 0 77 -4,-2.7 4,-2.4 1,-0.2 -2,-0.2 0.925 111.3 46.9 -57.4 -41.4 3.4 -2.6 14.6 80 11 C S H X S+ 0 0 82 -4,-2.2 4,-2.0 1,-0.2 -1,-0.2 0.916 111.9 50.1 -67.3 -45.1 5.4 -5.2 16.6 81 12 C N H X S+ 0 0 66 -4,-2.4 4,-2.3 2,-0.2 -2,-0.2 0.920 112.1 47.2 -58.3 -47.3 7.6 -2.5 18.1 82 13 C F H X S+ 0 0 34 -4,-3.0 4,-2.6 1,-0.2 -2,-0.2 0.878 109.6 53.7 -67.8 -36.2 4.7 -0.3 19.1 83 14 C G H X S+ 0 0 44 -4,-2.4 4,-2.3 -5,-0.3 -1,-0.2 0.905 109.9 47.5 -61.0 -40.3 2.9 -3.3 20.6 84 15 C A H X S+ 0 0 55 -4,-2.0 4,-2.3 2,-0.2 -2,-0.2 0.911 111.3 50.7 -68.0 -43.1 5.9 -4.2 22.8 85 16 C I H X S+ 0 0 16 -4,-2.3 4,-2.9 2,-0.2 5,-0.2 0.937 109.9 50.4 -61.2 -41.8 6.3 -0.5 23.9 86 17 C S H X S+ 0 0 61 -4,-2.6 4,-2.6 1,-0.2 -2,-0.2 0.923 110.5 50.2 -62.3 -44.6 2.6 -0.4 24.9 87 18 C S H X S+ 0 0 79 -4,-2.3 4,-2.0 1,-0.2 -1,-0.2 0.902 113.4 44.6 -58.5 -46.3 3.0 -3.7 26.9 88 19 C V H X S+ 0 0 46 -4,-2.3 4,-2.7 2,-0.2 -2,-0.2 0.906 113.2 50.3 -66.5 -42.7 6.1 -2.4 28.7 89 20 C L H X S+ 0 0 14 -4,-2.9 4,-2.9 1,-0.2 5,-0.2 0.920 110.2 50.9 -59.8 -45.2 4.5 1.1 29.4 90 21 C N H X S+ 0 0 93 -4,-2.6 4,-2.4 -5,-0.2 -1,-0.2 0.914 110.4 49.3 -59.5 -41.9 1.4 -0.6 30.8 91 22 C D H X S+ 0 0 77 -4,-2.0 4,-2.3 1,-0.2 -2,-0.2 0.934 112.6 47.6 -65.3 -44.9 3.5 -2.8 33.1 92 23 C I H X S+ 0 0 0 -4,-2.7 4,-2.9 2,-0.2 5,-0.2 0.918 111.9 49.3 -60.6 -43.0 5.4 0.2 34.3 93 24 C L H X S+ 0 0 41 -4,-2.9 4,-2.4 1,-0.2 -2,-0.2 0.924 111.5 49.9 -65.4 -41.7 2.2 2.3 34.9 94 25 C S H X S+ 0 0 65 -4,-2.4 4,-1.4 -5,-0.2 -1,-0.2 0.890 113.4 45.3 -59.8 -44.1 0.7 -0.6 36.8 95 26 C R H X S+ 0 0 45 -4,-2.3 4,-2.2 1,-0.2 -2,-0.2 0.875 110.9 52.9 -71.7 -37.5 3.8 -1.0 39.0 96 27 C L H X S+ 0 0 1 -4,-2.9 4,-2.6 1,-0.2 -2,-0.2 0.908 105.3 55.5 -62.9 -43.3 4.0 2.7 39.6 97 28 C D H X S+ 0 0 87 -4,-2.4 4,-1.0 -5,-0.2 -1,-0.2 0.914 109.8 46.9 -54.7 -42.2 0.4 2.8 40.8 98 29 C K H < S+ 0 0 155 -4,-1.4 4,-0.4 2,-0.2 3,-0.3 0.895 112.1 47.8 -69.8 -42.5 1.2 0.1 43.4 99 30 C V H >X S+ 0 0 8 -4,-2.2 3,-1.7 1,-0.2 4,-0.9 0.901 105.4 59.3 -66.8 -39.5 4.4 1.8 44.7 100 31 C E H 3< S+ 0 0 68 -4,-2.6 3,-0.3 1,-0.3 -1,-0.2 0.818 102.9 54.7 -57.6 -28.7 2.6 5.2 45.0 101 32 C A T 3< S+ 0 0 85 -4,-1.0 -1,-0.3 -3,-0.3 -2,-0.2 0.607 106.0 51.4 -78.8 -15.0 0.2 3.5 47.4 102 33 C E T <4 0 0 138 -3,-1.7 -1,-0.2 -4,-0.4 -2,-0.2 0.550 360.0 360.0-101.0 -11.3 3.0 2.3 49.6 103 34 C V < 0 0 78 -4,-0.9 -2,-0.2 -3,-0.3 -3,-0.1 0.324 360.0 360.0-116.3 360.0 4.7 5.6 50.1