data_17472 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; TRPV5 C-terminal peptide ; _BMRB_accession_number 17472 _BMRB_flat_file_name bmr17472.str _Entry_type original _Submission_date 2011-02-16 _Accession_date 2011-02-16 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kovalevskaya Nadezda V. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 43 "13C chemical shifts" 39 "15N chemical shifts" 25 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2011-07-01 update BMRB 'update entry citation' 2011-05-19 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Molecular Mechanisms of Calmodulin Action on TRPV5 and Modulation by Parathyroid Hormone.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 21576356 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 'de Groot' Theun . . 2 Kovalevskaya Nadezda V. . 3 Verkaart Sjoerd . . 4 Schilderink Nathalie . . 5 Felici Marco . . 6 'van der Hagen' Eline A.E. . 7 Bindels Rene J.M. . 8 Vuister Geerten W. . 9 Hoenderop Joost G. . stop_ _Journal_abbreviation 'Mol. Cell. Biol.' _Journal_name_full 'Molecular and cellular biology' _Journal_volume 31 _Journal_issue 14 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2845 _Page_last 2853 _Year 2011 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name trpv5_peptide _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label trpv5 $trpv5 stop_ _System_molecular_weight 5000 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_trpv5 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common trpv5 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 43 _Mol_residue_sequence ; GSSHRGWEILRQNTLGHLNL GLNLSEGDGEEVYHFLEHHH HHH ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 SER 3 SER 4 HIS 5 ARG 6 GLY 7 TRP 8 GLU 9 ILE 10 LEU 11 ARG 12 GLN 13 ASN 14 THR 15 LEU 16 GLY 17 HIS 18 LEU 19 ASN 20 LEU 21 GLY 22 LEU 23 ASN 24 LEU 25 SER 26 GLU 27 GLY 28 ASP 29 GLY 30 GLU 31 GLU 32 VAL 33 TYR 34 HIS 35 PHE 36 LEU 37 GLU 38 HIS 39 HIS 40 HIS 41 HIS 42 HIS 43 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-06-13 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value EMBL CAB96365 "epithelial calcium channel [Homo sapiens]" 79.07 729 100.00 100.00 2.03e-06 EMBL CAD32312 "transient receptor potential cation channel subfamily V, member 5 [Homo sapiens]" 79.07 729 100.00 100.00 2.48e-06 GB AAL04015 "calcium transport protein CaT2 [Homo sapiens]" 79.07 729 100.00 100.00 2.03e-06 GB AAO13488 "transient receptor potential cation channel, subfamily V, member 5 [Homo sapiens]" 79.07 729 100.00 100.00 2.03e-06 GB EAL23777 "transient receptor potential cation channel, subfamily V, member 5 [Homo sapiens]" 79.07 729 100.00 100.00 2.03e-06 GB EAW51896 "transient receptor potential cation channel, subfamily V, member 5 [Homo sapiens]" 79.07 729 100.00 100.00 2.03e-06 GB EHH52584 "hypothetical protein EGM_13045 [Macaca fascicularis]" 79.07 776 97.06 100.00 6.82e-14 REF NP_062815 "transient receptor potential cation channel subfamily V member 5 [Homo sapiens]" 79.07 729 100.00 100.00 2.50e-06 REF XP_001089988 "PREDICTED: transient receptor potential cation channel subfamily V member 5 isoform 1 [Macaca mulatta]" 79.07 776 97.06 100.00 3.63e-14 REF XP_003270895 "PREDICTED: transient receptor potential cation channel subfamily V member 5 [Nomascus leucogenys]" 79.07 729 97.06 100.00 9.21e-06 REF XP_003820606 "PREDICTED: transient receptor potential cation channel subfamily V member 5 isoform X2 [Pan paniscus]" 79.07 729 97.06 97.06 3.93e-14 REF XP_003896799 "PREDICTED: transient receptor potential cation channel subfamily V member 5 isoform X1 [Papio anubis]" 79.07 729 100.00 100.00 2.55e-06 SP Q9NQA5 "RecName: Full=Transient receptor potential cation channel subfamily V member 5; Short=TrpV5; AltName: Full=Calcium transport pr" 79.07 729 100.00 100.00 2.03e-06 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $trpv5 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $trpv5 'recombinant technology' . Escherichia coli . pGEV2 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $trpv5 150 uM '[U-99% 13C; U-99% 15N]' 'sodium azide' 0.01% v/v 'natural abundance' 'calcium chloride' 10 mM 'natural abundance' 'ammonium acetate' 10 mM 'natural abundance' H2O 95 % 'natural abundance' D2O 5 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CcpNmr_Analysis _Saveframe_category software _Name ANALYSIS _Version 2.1.5. loop_ _Vendor _Address _Electronic_address 'CCPN project' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 800 _Details cryoprobe save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HBHA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HBHA(CO)NH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 . pH temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label water C 13 protons ppm 4.773 internal indirect . . . 0.251449530 $entry_citation $entry_citation water H 1 protons ppm 4.773 internal direct . . . 1.000000000 $entry_citation $entry_citation water N 15 protons ppm 4.773 internal indirect . . . 0.101329118 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCACB' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name trpv5 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 ARG H H 8.384 0.001 1 2 5 5 ARG HA H 4.225 0.000 1 3 5 5 ARG CA C 56.779 0.000 1 4 5 5 ARG CB C 30.724 0.000 1 5 5 5 ARG N N 122.561 0.027 1 6 6 6 GLY H H 8.540 0.002 1 7 6 6 GLY CA C 45.400 0.000 1 8 6 6 GLY N N 110.309 0.044 1 9 7 7 TRP H H 7.906 0.001 1 10 7 7 TRP CA C 57.665 0.000 1 11 7 7 TRP N N 120.462 0.033 1 12 8 8 GLU H H 8.302 0.001 1 13 8 8 GLU HA H 4.074 0.000 1 14 8 8 GLU CA C 57.462 0.000 1 15 8 8 GLU N N 121.753 0.007 1 16 9 9 ILE H H 7.888 0.001 1 17 9 9 ILE HA H 4.010 0.000 1 18 9 9 ILE CA C 62.389 0.000 1 19 9 9 ILE N N 120.569 0.070 1 20 10 10 LEU H H 8.023 0.001 1 21 10 10 LEU HA H 4.279 0.000 1 22 10 10 LEU CA C 55.933 0.000 1 23 10 10 LEU CB C 42.280 0.000 1 24 10 10 LEU N N 124.414 0.031 1 25 11 11 ARG H H 8.163 0.001 1 26 11 11 ARG CA C 56.798 0.000 1 27 11 11 ARG N N 121.143 0.027 1 28 13 13 ASN H H 8.425 0.001 1 29 13 13 ASN HA H 4.731 0.000 1 30 13 13 ASN CA C 53.601 0.000 1 31 13 13 ASN N N 119.342 0.030 1 32 14 14 THR H H 8.071 0.001 1 33 14 14 THR HA H 4.287 0.000 1 34 14 14 THR CA C 62.308 0.000 1 35 14 14 THR CB C 69.568 0.000 1 36 14 14 THR N N 114.077 0.023 1 37 15 15 LEU H H 8.167 0.014 1 38 15 15 LEU HA H 4.291 0.000 1 39 15 15 LEU CA C 56.010 0.000 1 40 15 15 LEU CB C 42.266 0.000 1 41 15 15 LEU N N 123.411 0.127 1 42 16 16 GLY H H 8.286 0.001 1 43 16 16 GLY CA C 45.546 0.000 1 44 16 16 GLY N N 108.840 0.026 1 45 17 17 HIS H H 8.223 0.002 1 46 17 17 HIS HA H 4.657 0.000 1 47 17 17 HIS CA C 55.819 0.000 1 48 17 17 HIS CB C 29.520 0.000 1 49 17 17 HIS N N 117.991 0.013 1 50 21 21 GLY H H 8.359 0.001 1 51 21 21 GLY HA2 H 4.295 0.000 2 52 21 21 GLY CA C 45.407 0.000 1 53 21 21 GLY N N 108.808 0.031 1 54 25 25 SER H H 8.323 0.001 1 55 25 25 SER HA H 4.440 0.000 1 56 25 25 SER CA C 58.412 0.000 1 57 25 25 SER CB C 63.825 0.000 1 58 25 25 SER N N 116.187 0.025 1 59 26 26 GLU H H 8.363 0.001 1 60 26 26 GLU HA H 4.293 0.000 1 61 26 26 GLU CA C 57.090 0.000 1 62 26 26 GLU CB C 30.480 0.000 1 63 26 26 GLU N N 122.472 0.016 1 64 27 27 GLY H H 8.346 0.001 1 65 27 27 GLY CA C 45.488 0.000 1 66 27 27 GLY N N 109.672 0.029 1 67 29 29 GLY H H 8.422 0.001 1 68 29 29 GLY CA C 45.666 0.000 1 69 29 29 GLY N N 109.308 0.047 1 70 30 30 GLU H H 8.275 0.012 1 71 30 30 GLU CA C 56.936 0.000 1 72 30 30 GLU CB C 30.362 0.000 1 73 30 30 GLU N N 120.389 0.026 1 74 31 31 GLU H H 8.510 0.007 1 75 31 31 GLU HA H 4.189 0.000 1 76 31 31 GLU CB C 30.082 0.000 1 77 31 31 GLU N N 121.290 0.106 1 78 32 32 VAL H H 7.909 0.001 1 79 32 32 VAL HA H 3.903 0.000 1 80 32 32 VAL CA C 63.074 0.000 1 81 32 32 VAL CB C 32.728 0.000 1 82 32 32 VAL N N 120.049 0.006 1 83 33 33 TYR H H 7.983 0.001 1 84 33 33 TYR HA H 4.390 0.000 1 85 33 33 TYR CA C 58.838 0.000 1 86 33 33 TYR CB C 38.675 0.000 1 87 33 33 TYR N N 121.868 0.035 1 88 34 34 HIS H H 8.164 0.002 1 89 34 34 HIS HA H 4.558 0.000 1 90 34 34 HIS CA C 55.997 0.000 1 91 34 34 HIS CB C 29.183 0.000 1 92 34 34 HIS N N 119.594 0.031 1 93 35 35 PHE H H 8.071 0.001 1 94 35 35 PHE HA H 4.457 0.000 1 95 35 35 PHE CA C 58.742 0.000 1 96 35 35 PHE CB C 39.438 0.000 1 97 35 35 PHE N N 120.777 0.020 1 98 36 36 LEU H H 8.184 0.021 1 99 36 36 LEU HA H 4.172 0.000 1 100 36 36 LEU CA C 55.577 0.000 1 101 36 36 LEU CB C 42.447 0.000 1 102 36 36 LEU N N 122.529 0.068 1 103 37 37 GLU H H 8.112 0.001 1 104 37 37 GLU HA H 4.090 0.000 1 105 37 37 GLU CA C 56.879 0.000 1 106 37 37 GLU CB C 30.381 0.000 1 107 37 37 GLU N N 120.518 0.035 1 stop_ save_