data_17511 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper GCN4 coiled coil and x-form conformations. ; _BMRB_accession_number 17511 _BMRB_flat_file_name bmr17511.str _Entry_type original _Submission_date 2011-03-06 _Accession_date 2011-03-06 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Chemical shift assignments of two conformations of recombinant 36-residue LZ-GCN4. Coiled coil (dimer) chemical shifts at pH 3.2 and 7.1. X-form (monomer) chemical shifts - only at pH 3.2' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Nikolaev Yaroslav . . 2 Pervushin Konstantin . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 3 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 173 "13C chemical shifts" 69 "15N chemical shifts" 104 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2011-04-08 update BMRB 'update entry citation' 2011-04-04 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'NMR spin state exchange spectroscopy reveals equilibrium of two distinct conformations of leucine zipper GCN4 in solution.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17469817 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Nikolaev Yaroslav . . 2 Pervushin Konstantin . . stop_ _Journal_abbreviation 'J. Am. Chem. Soc.' _Journal_name_full 'Journal of the American Chemical Society' _Journal_volume 129 _Journal_issue 20 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 6461 _Page_last 6469 _Year 2007 _Details . loop_ _Keyword bzip exchange 'leucine zipper' NMR stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'LZ-GCN4 dimer' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'LZ-GCN4 dimer, chain 1' $LZ-GCN4 'LZ-GCN4 dimer, chain 2' $LZ-GCN4 'LZ-GCN4 monomer' $LZ-GCN4 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_LZ-GCN4 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common LZ-GCN4 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 36 _Mol_residue_sequence ; GLQRMKQLEDKVEELLSKNY HLENEVARLKKLVGER ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 LEU 3 GLN 4 ARG 5 MET 6 LYS 7 GLN 8 LEU 9 GLU 10 ASP 11 LYS 12 VAL 13 GLU 14 GLU 15 LEU 16 LEU 17 SER 18 LYS 19 ASN 20 TYR 21 HIS 22 LEU 23 GLU 24 ASN 25 GLU 26 VAL 27 ALA 28 ARG 29 LEU 30 LYS 31 LYS 32 LEU 33 VAL 34 GLY 35 GLU 36 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1396 "transcriptional regulator GCN4" 97.22 61 100.00 100.00 4.53e-14 BMRB 1397 "transcriptional regulator GCN4" 97.22 61 100.00 100.00 4.53e-14 BMRB 1398 "transcriptional regulator GCN4" 97.22 61 100.00 100.00 4.53e-14 BMRB 1399 "transcriptional regulator GCN4" 97.22 61 100.00 100.00 4.53e-14 BMRB 1451 "transcriptional regulator GCN4" 97.22 61 100.00 100.00 4.53e-14 BMRB 1452 "transcriptional regulator GCN4" 97.22 61 100.00 100.00 4.53e-14 BMRB 1453 "transcriptional regulator GCN4" 97.22 61 100.00 100.00 4.53e-14 BMRB 1454 "transcriptional regulator GCN4" 97.22 61 100.00 100.00 4.53e-14 BMRB 15316 GCN4p-wt 86.11 33 100.00 100.00 1.15e-10 BMRB 2062 "transcriptional regulator GCN4-p2N" 88.89 37 100.00 100.00 2.19e-11 BMRB 371 "transcriptional regulator GCN4" 88.89 33 100.00 100.00 1.98e-11 PDB 1DGC "The X-Ray Structure Of The Gcn4-Bzip Bound To AtfCREB SITE Dna Shows The Complex Depends On Dna Flexibility" 97.22 62 100.00 100.00 4.58e-14 PDB 1IJ2 "Gcn4-Pvtl Coiled-Coil Trimer With Threonine At The A(16) Position" 91.67 34 96.97 96.97 1.72e-11 PDB 1IJ3 "Gcn4-Pvsl Coiled-Coil Trimer With Serine At The A(16) Position" 91.67 34 96.97 100.00 1.67e-11 PDB 1LD4 "Placement Of The Structural Proteins In Sindbis Virus" 97.22 57 100.00 100.00 5.07e-14 PDB 1LLM "Crystal Structure Of A Zif23-Gcn4 Chimera Bound To Dna" 80.56 88 100.00 100.00 2.04e-09 PDB 1NKN "Visualizing An Unstable Coiled Coil: The Crystal Structure Of An N-Terminal Segment Of The Scallop Myosin Rod" 88.89 89 100.00 100.00 2.61e-11 PDB 1RB4 "Antiparallel Trimer Of Gcn4-Leucine Zipper Core Mutant As N16a Tetragonal Automatic Solution" 91.67 33 96.97 96.97 3.31e-11 PDB 1RB5 "Antiparallel Trimer Of Gcn4-Leucine Zipper Core Mutant As N16a Trigonal Form" 91.67 34 96.97 96.97 3.35e-11 PDB 1RB6 "Antiparallel Trimer Of Gcn4-Leucine Zipper Core Mutant As N16a Tetragonal Form" 91.67 34 96.97 96.97 3.35e-11 PDB 1SWI "Gcn4-Leucine Zipper Core Mutant As N16a Complexed With Benzene" 91.67 33 96.97 96.97 3.31e-11 PDB 1YSA "The Gcn4 Basic Region Leucine Zipper Binds Dna As A Dimer Of Uninterrupted Alpha Helices: Crystal Structure Of The Protein-Dna " 97.22 58 100.00 100.00 5.64e-14 PDB 1ZII "Gcn4-Leucine Zipper Core Mutant Asn16aba In The Dimeric State" 91.67 34 96.97 96.97 3.85e-11 PDB 1ZIJ "Gcn4-Leucine Zipper Core Mutant Asn16aba In The Trimeric State" 91.67 34 96.97 96.97 3.85e-11 PDB 1ZIK "Gcn4-Leucine Zipper Core Mutant Asn16lys In The Dimeric State" 91.67 33 96.97 96.97 3.21e-11 PDB 1ZIL "Gcn4-Leucine Zipper Core Mutant Asn16gln In The Dimeric State" 91.67 33 96.97 96.97 2.51e-11 PDB 1ZIM "Gcn4-Leucine Zipper Core Mutant Asn16gln In The Trimeric State" 91.67 34 96.97 96.97 2.53e-11 PDB 1ZTA "The Solution Structure Of A Leucine-Zipper Motif Peptide" 97.22 35 100.00 100.00 1.64e-13 PDB 2AHP "Gcn4 Leucine Zipper, Mutation Of Lys15 To Epsilon-Azido-Lys" 91.67 34 96.97 96.97 2.59e-11 PDB 2DGC "Gcn4 Basic Domain, Leucine Zipper Complexed With AtfCREB Site Dna" 97.22 63 100.00 100.00 4.98e-14 PDB 2ZTA "X-Ray Structure Of The Gcn4 Leucine Zipper, A Two-Stranded, Parallel Coiled Coil" 91.67 34 100.00 100.00 3.45e-12 PDB 3BAS "Crystal Structure Of The N-Terminal Region Of The Scallop Myosin Rod, Monoclinic (C2) Form" 88.89 89 100.00 100.00 2.61e-11 PDB 3BAT "Crystal Structure Of The N-Terminal Region Of The Scallop Myosin Rod, Monoclinic (P21) Form" 88.89 89 100.00 100.00 2.61e-11 PDB 3I5C "Crystal Structure Of A Fusion Protein Containing The Leucine Zipper Of Gcn4 And The Ggdef Domain Of Wspr From Pseudomonas Aerug" 83.33 206 100.00 100.00 8.85e-10 PDB 3K7Z "Gcn4-Leucine Zipper Core Mutant As N16a Trigonal Automatic Solution" 91.67 33 96.97 96.97 3.31e-11 PDB 4DMD "Gcn4 Leucine Zipper Domain In A Dimeric Oligomerization State" 91.67 34 100.00 100.00 3.45e-12 PDB 4DME "Gcn4 Leucine Zipper Domain In A Trimeric Oligomerization State" 91.67 35 100.00 100.00 3.49e-12 PDB 4NIZ "Gcn4-p1 Single Val9 To Aminobutyric Acid Mutant" 91.67 35 96.97 96.97 2.56e-11 PDB 4NJ0 "Gcn4-p1 Single Val9 To Ile Mutant" 91.67 35 96.97 100.00 4.76e-12 PDB 4TL1 "Gcn4-p1 With Mutation To 1-aminocyclohexanecarboxylic Acid At Residue 10" 91.67 35 96.97 96.97 1.92e-11 DBJ GAA22815 "K7_Gcn4p [Saccharomyces cerevisiae Kyokai no. 7]" 97.22 281 100.00 100.00 3.28e-12 EMBL CAE52206 "Gcn4p [Saccharomyces cerevisiae]" 97.22 281 100.00 100.00 2.40e-12 EMBL CAE52207 "Gcn4p [Saccharomyces cerevisiae]" 97.22 281 100.00 100.00 2.40e-12 EMBL CAE52208 "Gcn4p [Saccharomyces cerevisiae]" 97.22 281 100.00 100.00 2.04e-12 EMBL CAE52209 "Gcn4p [Saccharomyces cerevisiae]" 97.22 281 100.00 100.00 2.40e-12 EMBL CAE52210 "Gcn4p [Saccharomyces cerevisiae]" 97.22 281 100.00 100.00 3.28e-12 GB AAA34640 "GCN4 protein [Saccharomyces cerevisiae]" 97.22 281 100.00 100.00 2.50e-12 GB AAB64486 "Transcriptional activator of amino acid biosynthetic genes [Saccharomyces cerevisiae]" 97.22 281 100.00 100.00 2.50e-12 GB AAK07887 "cI-GCN4 repressor fusion protein [Cloning vector pJH370]" 88.89 166 100.00 100.00 3.25e-11 GB AAL09032 "GCN4 [Saccharomyces cerevisiae]" 91.67 35 100.00 100.00 2.25e-12 GB AHY75543 "Gcn4p [Saccharomyces cerevisiae YJM993]" 97.22 281 100.00 100.00 2.50e-12 REF NP_010907 "amino acid starvation-responsive transcription factor GCN4 [Saccharomyces cerevisiae S288c]" 97.22 281 100.00 100.00 2.50e-12 SP P03069 "RecName: Full=General control protein GCN4; AltName: Full=Amino acid biosynthesis regulatory protein" 97.22 281 100.00 100.00 2.50e-12 TPG DAA07643 "TPA: amino acid starvation-responsive transcription factor GCN4 [Saccharomyces cerevisiae S288c]" 97.22 281 100.00 100.00 2.50e-12 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $LZ-GCN4 'baker's yeast' 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $LZ-GCN4 'recombinant technology' 'Escherichia coli' Escherichia coli . pQE30 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_LZ-GCN4_NC _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $LZ-GCN4 0.3 mM '[U-13C; U-15N]' 'potassium chloride' 80 mM 'natural abundance' 'sodium acetate' 40 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AMX _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AMX _Field_strength 900 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_HNCACB_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $LZ-GCN4_NC save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $LZ-GCN4_NC save_ save_3D_1H-15N_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $LZ-GCN4_NC save_ save_NMR_spectrometer_expt _Saveframe_category NMR_applied_experiment _Experiment_name . _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_pH_3.2_310K _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 3.2 . pH pressure 1 . atm temperature 310 . K stop_ save_ save_pH_7.1_310K _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.1 . pH temperature 310 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 . indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_LZ-GCN4_coiled_coil_shifts_at_pH_7.1 _Saveframe_category assigned_chemical_shifts _Details 'Assigned using pH titration series and 3D-15N-NOESY data.' loop_ _Experiment_label '2D 1H-15N HSQC' '3D 1H-15N NOESY' stop_ loop_ _Sample_label $LZ-GCN4_NC stop_ _Sample_conditions_label $pH_7.1_310K _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'LZ-GCN4 dimer, chain 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 LEU H H 8.672 0.020 1 2 2 2 LEU N N 121.986 0.3 1 3 3 3 GLN H H 8.711 0.020 1 4 3 3 GLN N N 122.178 0.3 1 5 4 4 ARG H H 8.363 0.020 1 6 4 4 ARG N N 121.455 0.3 1 7 5 5 MET H H 8.180 0.020 1 8 5 5 MET N N 118.270 0.3 1 9 6 6 LYS H H 7.707 0.020 1 10 6 6 LYS N N 119.008 0.3 1 11 7 7 GLN H H 7.848 0.020 1 12 7 7 GLN N N 117.360 0.3 1 13 8 8 LEU H H 8.107 0.020 1 14 8 8 LEU N N 120.568 0.3 1 15 9 9 GLU H H 8.529 0.020 1 16 9 9 GLU N N 120.224 0.3 1 17 10 10 ASP H H 8.590 0.020 1 18 10 10 ASP N N 119.855 0.3 1 19 11 11 LYS H H 8.019 0.020 1 20 11 11 LYS N N 123.259 0.3 1 21 12 12 VAL H H 8.459 0.020 1 22 12 12 VAL N N 119.911 0.3 1 23 13 13 GLU H H 7.683 0.020 1 24 13 13 GLU N N 117.617 0.3 1 25 14 14 GLU H H 8.195 0.020 1 26 14 14 GLU N N 120.897 0.3 1 27 15 15 LEU H H 8.687 0.020 1 28 15 15 LEU N N 120.324 0.3 1 29 16 16 LEU H H 9.054 0.020 1 30 16 16 LEU N N 122.101 0.3 1 31 17 17 SER H H 7.814 0.020 1 32 17 17 SER N N 114.703 0.3 1 33 18 18 LYS H H 8.248 0.020 1 34 18 18 LYS N N 122.047 0.3 1 35 19 19 ASN H H 8.808 0.020 1 36 19 19 ASN N N 119.373 0.3 1 37 20 20 TYR H H 8.163 0.020 1 38 20 20 TYR N N 120.325 0.3 1 39 21 21 HIS H H 7.806 0.020 1 40 21 21 HIS N N 118.303 0.3 1 41 22 22 LEU H H 8.563 0.020 1 42 22 22 LEU N N 120.431 0.3 1 43 23 23 GLU H H 8.853 0.020 1 44 23 23 GLU N N 119.623 0.3 1 45 24 24 ASN H H 7.741 0.020 1 46 24 24 ASN N N 119.016 0.3 1 47 25 25 GLU H H 8.167 0.020 1 48 25 25 GLU N N 124.016 0.3 1 49 26 26 VAL H H 8.695 0.020 1 50 26 26 VAL N N 120.083 0.3 1 51 27 27 ALA H H 7.742 0.020 1 52 27 27 ALA N N 120.030 0.3 1 53 28 28 ARG H H 7.969 0.020 1 54 28 28 ARG N N 119.688 0.3 1 55 29 29 LEU H H 8.473 0.020 1 56 29 29 LEU N N 120.391 0.3 1 57 30 30 LYS H H 9.005 0.020 1 58 30 30 LYS N N 118.713 0.3 1 59 31 31 LYS H H 7.385 0.020 1 60 31 31 LYS N N 117.644 0.3 1 61 32 32 LEU H H 7.484 0.020 1 62 32 32 LEU N N 118.953 0.3 1 63 33 33 VAL H H 7.761 0.020 1 64 33 33 VAL N N 113.629 0.3 1 65 34 34 GLY H H 7.859 0.020 1 66 34 34 GLY N N 109.162 0.3 1 67 35 35 GLU H H 7.955 0.020 1 68 35 35 GLU N N 120.827 0.3 1 69 36 36 ARG H H 7.908 0.020 1 70 36 36 ARG N N 127.105 0.3 1 stop_ save_ save_LZ-GCN4_coiled_coil_and_x-form_shifts_at_pH_3.2_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D HNCACB' stop_ loop_ _Sample_label $LZ-GCN4_NC stop_ _Sample_conditions_label $pH_3.2_310K _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'LZ-GCN4 dimer, chain 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 2 LEU H H 8.650 0.020 1 2 1 2 LEU HA H 4.402 0.020 1 3 1 2 LEU CA C 55.613 0.3 1 4 1 2 LEU N N 122.769 0.3 1 5 2 3 GLN H H 8.681 0.020 1 6 2 3 GLN HA H 4.294 0.020 1 7 2 3 GLN CA C 57.202 0.3 1 8 2 3 GLN N N 122.816 0.3 1 9 3 4 ARG H H 8.402 0.020 1 10 3 4 ARG HA H 4.223 0.020 1 11 3 4 ARG CA C 57.978 0.3 1 12 3 4 ARG N N 122.108 0.3 1 13 4 5 MET H H 8.288 0.020 1 14 4 5 MET HA H 4.123 0.020 1 15 4 5 MET CA C 58.622 0.3 1 16 4 5 MET N N 119.300 0.3 1 17 5 6 LYS H H 7.847 0.020 1 18 5 6 LYS HA H 4.064 0.020 1 19 5 6 LYS CA C 58.762 0.3 1 20 5 6 LYS N N 119.812 0.3 1 21 6 7 GLN H H 7.883 0.020 1 22 6 7 GLN HA H 4.130 0.020 1 23 6 7 GLN CA C 58.887 0.3 1 24 6 7 GLN N N 118.336 0.3 1 25 7 8 LEU H H 8.059 0.020 1 26 7 8 LEU HA H 4.146 0.020 1 27 7 8 LEU CA C 57.801 0.3 1 28 7 8 LEU N N 121.224 0.3 1 29 8 9 GLU H H 8.443 0.020 1 30 8 9 GLU HA H 3.969 0.020 1 31 8 9 GLU CA C 59.223 0.3 1 32 8 9 GLU N N 119.813 0.3 1 33 9 10 ASP H H 8.494 0.020 1 34 9 10 ASP HA H 4.470 0.020 1 35 9 10 ASP CA C 56.214 0.3 1 36 9 10 ASP N N 119.774 0.3 1 37 10 11 LYS H H 7.997 0.020 1 38 10 11 LYS HA H 4.195 0.020 1 39 10 11 LYS CA C 58.708 0.3 1 40 10 11 LYS N N 123.250 0.3 1 41 11 12 VAL H H 8.370 0.020 1 42 11 12 VAL HA H 3.447 0.020 1 43 11 12 VAL CA C 67.525 0.3 1 44 11 12 VAL N N 120.122 0.3 1 45 12 13 GLU H H 7.832 0.020 1 46 12 13 GLU HA H 4.060 0.020 1 47 12 13 GLU CA C 59.179 0.3 1 48 12 13 GLU N N 117.543 0.3 1 49 13 14 GLU H H 8.264 0.020 1 50 13 14 GLU HA H 4.174 0.020 1 51 13 14 GLU CA C 58.887 0.3 1 52 13 14 GLU N N 120.938 0.3 1 53 14 15 LEU H H 8.653 0.020 1 54 14 15 LEU HA H 4.038 0.020 1 55 14 15 LEU CA C 57.843 0.3 1 56 14 15 LEU N N 121.712 0.3 1 57 15 16 LEU H H 8.914 0.020 1 58 15 16 LEU HA H 4.059 0.020 1 59 15 16 LEU CA C 58.052 0.3 1 60 15 16 LEU N N 122.604 0.3 1 61 16 17 SER H H 7.759 0.020 1 62 16 17 SER HA H 4.369 0.020 1 63 16 17 SER CA C 61.434 0.3 1 64 16 17 SER N N 114.970 0.3 1 65 17 18 LYS H H 8.308 0.020 1 66 17 18 LYS HA H 4.238 0.020 1 67 17 18 LYS CA C 59.513 0.3 1 68 17 18 LYS N N 123.131 0.3 1 69 18 19 ASN H H 8.817 0.020 1 70 18 19 ASN HA H 4.402 0.020 1 71 18 19 ASN CA C 57.175 0.3 1 72 18 19 ASN N N 120.293 0.3 1 73 19 20 TYR H H 8.308 0.020 1 74 19 20 TYR HA H 4.381 0.020 1 75 19 20 TYR CA C 60.682 0.3 1 76 19 20 TYR N N 121.033 0.3 1 77 20 21 HIS H H 8.016 0.020 1 78 20 21 HIS HA H 4.404 0.020 1 79 20 21 HIS CA C 59.221 0.3 1 80 20 21 HIS N N 117.879 0.3 1 81 21 22 LEU H H 8.724 0.020 1 82 21 22 LEU HA H 4.085 0.020 1 83 21 22 LEU CA C 58.093 0.3 1 84 21 22 LEU N N 121.897 0.3 1 85 22 23 GLU H H 8.827 0.020 1 86 22 23 GLU HA H 3.993 0.020 1 87 22 23 GLU CA C 59.269 0.3 1 88 22 23 GLU N N 119.773 0.3 1 89 23 24 ASN H H 7.789 0.020 1 90 23 24 ASN HA H 4.471 0.020 1 91 23 24 ASN CA C 55.956 0.3 1 92 23 24 ASN N N 119.554 0.3 1 93 24 25 GLU H H 8.045 0.020 1 94 24 25 GLU HA H 4.175 0.020 1 95 24 25 GLU CA C 58.996 0.3 1 96 24 25 GLU N N 122.993 0.3 1 97 25 26 VAL H H 8.624 0.020 1 98 25 26 VAL HA H 3.420 0.020 1 99 25 26 VAL CA C 67.405 0.3 1 100 25 26 VAL N N 120.389 0.3 1 101 26 27 ALA H H 7.754 0.020 1 102 26 27 ALA HA H 4.036 0.020 1 103 26 27 ALA CA C 55.296 0.3 1 104 26 27 ALA N N 120.824 0.3 1 105 27 28 ARG H H 7.827 0.020 1 106 27 28 ARG HA H 4.049 0.020 1 107 27 28 ARG CA C 59.189 0.3 1 108 27 28 ARG N N 119.052 0.3 1 109 28 29 LEU H H 8.417 0.020 1 110 28 29 LEU HA H 4.061 0.020 1 111 28 29 LEU CA C 57.801 0.3 1 112 28 29 LEU N N 120.815 0.3 1 113 29 30 LYS H H 8.837 0.020 1 114 29 30 LYS HA H 3.863 0.020 1 115 29 30 LYS CA C 60.348 0.3 1 116 29 30 LYS N N 118.838 0.3 1 117 30 31 LYS H H 7.328 0.020 1 118 30 31 LYS HA H 4.153 0.020 1 119 30 31 LYS CA C 58.595 0.3 1 120 30 31 LYS N N 118.588 0.3 1 121 31 32 LEU H H 7.583 0.020 1 122 31 32 LEU HA H 4.218 0.020 1 123 31 32 LEU CA C 57.008 0.3 1 124 31 32 LEU N N 119.710 0.3 1 125 32 33 VAL H H 7.861 0.020 1 126 32 33 VAL HA H 4.002 0.020 1 127 32 33 VAL CA C 63.605 0.3 1 128 32 33 VAL N N 116.057 0.3 1 129 33 34 GLY H H 7.848 0.020 1 130 33 34 GLY HA2 H 3.997 0.020 1 131 33 34 GLY HA3 H 3.997 0.020 1 132 33 34 GLY CA C 45.692 0.3 1 133 33 34 GLY N N 109.272 0.3 1 134 34 35 GLU H H 7.912 0.020 1 135 34 35 GLU HA H 4.440 0.020 1 136 34 35 GLU CA C 55.505 0.3 1 137 34 35 GLU N N 120.295 0.3 1 138 35 36 ARG H H 8.001 0.020 1 139 35 36 ARG HA H 4.274 0.020 1 140 35 36 ARG CA C 56.423 0.3 1 141 35 36 ARG N N 125.524 0.3 1 stop_ save_ save_LZ-GCN4_coiled_coil_and_x-form_shifts_at_pH_3.2_3 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D HNCACB' stop_ loop_ _Sample_label $LZ-GCN4_NC stop_ _Sample_conditions_label $pH_3.2_310K _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'LZ-GCN4 monomer' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 101 2 LEU H H 8.533 0.020 1 2 101 2 LEU HA H 4.396 0.020 1 3 101 2 LEU CA C 55.220 0.3 1 4 101 2 LEU N N 122.481 0.3 1 5 102 3 GLN H H 8.515 0.020 1 6 102 3 GLN HA H 4.372 0.020 1 7 102 3 GLN CA C 55.323 0.3 1 8 102 3 GLN N N 122.825 0.3 1 9 103 4 ARG H H 8.401 0.020 1 10 103 4 ARG HA H 4.322 0.020 1 11 103 4 ARG CA C 55.733 0.3 1 12 103 4 ARG N N 123.433 0.3 1 13 104 5 MET H H 8.344 0.020 1 14 104 5 MET CA C 55.394 0.3 1 15 104 5 MET N N 121.975 0.3 1 16 105 6 LYS H H 8.279 0.020 1 17 105 6 LYS HA H 4.261 0.020 1 18 105 6 LYS CA C 56.269 0.3 1 19 105 6 LYS N N 123.172 0.3 1 20 106 7 GLN H H 8.315 0.020 1 21 106 7 GLN HA H 4.325 0.020 1 22 106 7 GLN CA C 55.776 0.3 1 23 106 7 GLN N N 121.762 0.3 1 24 107 8 LEU H H 8.205 0.020 1 25 107 8 LEU HA H 4.131 0.020 1 26 107 8 LEU CA C 55.102 0.3 1 27 107 8 LEU N N 123.806 0.3 1 28 108 9 GLU H H 8.276 0.020 1 29 108 9 GLU HA H 4.354 0.020 1 30 108 9 GLU CA C 55.501 0.3 1 31 108 9 GLU N N 121.216 0.3 1 32 109 10 ASP H H 8.338 0.020 1 33 109 10 ASP HA H 4.676 0.020 1 34 109 10 ASP CA C 53.205 0.3 1 35 109 10 ASP N N 120.903 0.3 1 36 110 11 LYS H H 8.190 0.020 1 37 110 11 LYS HA H 4.291 0.020 1 38 110 11 LYS CA C 56.593 0.3 1 39 110 11 LYS N N 122.218 0.3 1 40 111 12 VAL H H 7.977 0.020 1 41 111 12 VAL HA H 3.989 0.020 1 42 111 12 VAL CA C 63.044 0.3 1 43 111 12 VAL N N 120.929 0.3 1 44 112 13 GLU H H 8.218 0.020 1 45 112 13 GLU HA H 4.268 0.020 1 46 112 13 GLU CA C 56.333 0.3 1 47 112 13 GLU N N 122.753 0.3 1 48 114 15 LEU H H 8.038 0.020 1 49 114 15 LEU HA H 4.278 0.020 1 50 114 15 LEU CA C 55.846 0.3 1 51 114 15 LEU N N 122.652 0.3 1 52 115 16 LEU H H 8.023 0.020 1 53 115 16 LEU HA H 4.307 0.020 1 54 115 16 LEU CA C 55.258 0.3 1 55 115 16 LEU N N 122.074 0.3 1 56 116 17 SER H H 8.022 0.020 1 57 116 17 SER HA H 4.357 0.020 1 58 116 17 SER CA C 58.376 0.3 1 59 116 17 SER N N 115.922 0.3 1 60 117 18 LYS H H 8.117 0.020 1 61 117 18 LYS HA H 4.247 0.020 1 62 117 18 LYS CA C 56.461 0.3 1 63 117 18 LYS N N 123.035 0.3 1 64 118 19 ASN H H 8.170 0.020 1 65 118 19 ASN HA H 4.680 0.020 1 66 118 19 ASN CA C 52.724 0.3 1 67 118 19 ASN N N 118.860 0.3 1 68 119 20 TYR H H 7.978 0.020 1 69 119 20 TYR HA H 4.470 0.020 1 70 119 20 TYR CA C 58.017 0.3 1 71 119 20 TYR N N 121.564 0.3 1 72 120 21 HIS H H 8.295 0.020 1 73 120 21 HIS HA H 4.664 0.020 1 74 120 21 HIS CA C 55.156 0.3 1 75 120 21 HIS N N 120.199 0.3 1 76 121 22 LEU H H 8.064 0.020 1 77 121 22 LEU HA H 4.283 0.020 1 78 121 22 LEU CA C 55.123 0.3 1 79 121 22 LEU N N 123.220 0.3 1 80 122 23 GLU H H 8.324 0.020 1 81 122 23 GLU HA H 4.313 0.020 1 82 122 23 GLU CA C 55.798 0.3 1 83 122 23 GLU N N 121.267 0.3 1 84 123 24 ASN H H 8.315 0.020 1 85 123 24 ASN HA H 4.682 0.020 1 86 123 24 ASN CA C 53.208 0.3 1 87 123 24 ASN N N 119.698 0.3 1 88 124 25 GLU H H 8.208 0.020 1 89 124 25 GLU HA H 4.291 0.020 1 90 124 25 GLU CA C 56.252 0.3 1 91 124 25 GLU N N 121.291 0.3 1 92 125 26 VAL H H 7.993 0.020 1 93 125 26 VAL HA H 3.987 0.020 1 94 125 26 VAL CA C 62.963 0.3 1 95 125 26 VAL N N 120.981 0.3 1 96 126 27 ALA H H 8.154 0.020 1 97 126 27 ALA HA H 4.266 0.020 1 98 126 27 ALA CA C 52.773 0.3 1 99 126 27 ALA N N 126.359 0.3 1 100 127 28 ARG H H 8.052 0.020 1 101 127 28 ARG HA H 4.262 0.020 1 102 127 28 ARG CA C 56.293 0.3 1 103 127 28 ARG N N 119.867 0.3 1 104 128 29 LEU H H 8.001 0.020 1 105 128 29 LEU HA H 4.281 0.020 1 106 128 29 LEU CA C 55.153 0.3 1 107 128 29 LEU N N 122.451 0.3 1 108 129 30 LYS H H 8.086 0.020 1 109 129 30 LYS HA H 4.272 0.020 1 110 129 30 LYS CA C 56.229 0.3 1 111 129 30 LYS N N 121.986 0.3 1 112 130 31 LYS H H 8.052 0.020 1 113 130 31 LYS HA H 4.298 0.020 1 114 130 31 LYS CA C 56.170 0.3 1 115 130 31 LYS N N 122.349 0.3 1 116 131 32 LEU H H 8.110 0.020 1 117 131 32 LEU CA C 54.773 0.3 1 118 131 32 LEU N N 123.872 0.3 1 119 132 33 VAL H H 8.022 0.020 1 120 132 33 VAL HA H 4.014 0.020 1 121 132 33 VAL CA C 62.201 0.3 1 122 132 33 VAL N N 121.115 0.3 1 123 133 34 GLY H H 8.377 0.020 1 124 133 34 GLY HA2 H 3.991 0.020 1 125 133 34 GLY HA3 H 3.991 0.020 1 126 133 34 GLY CA C 44.774 0.3 1 127 133 34 GLY N N 112.776 0.3 1 128 134 35 GLU H H 8.073 0.020 1 129 134 35 GLU HA H 4.427 0.020 1 130 134 35 GLU CA C 55.343 0.3 1 131 134 35 GLU N N 120.616 0.3 1 132 135 36 ARG H H 8.183 0.020 1 133 135 36 ARG HA H 4.275 0.020 1 134 135 36 ARG CA C 56.038 0.3 1 135 135 36 ARG N N 125.981 0.3 1 stop_ save_