data_17722 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone (1H,13C,15N) chemical shifts for the two tandem Calcium Binding Domains (CBD12) of the Na+/Ca2+ exchanger in the Ca2+-bound state ; _BMRB_accession_number 17722 _BMRB_flat_file_name bmr17722.str _Entry_type original _Submission_date 2011-06-20 _Accession_date 2011-06-20 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Backbone (1H,15N,13C) chemical shifts of CBD12 in the Ca2+-bound state' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Salinas Roberto K. . 2 Bruschweiler-Li Lei . . 3 Johnson Eric . . 4 Bruschweiler Rafael . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 232 "13C chemical shifts" 373 "15N chemical shifts" 232 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-02-17 update BMRB 'update entry citation' 2011-08-16 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 15764 'Resonance assignments for CBD1 in the free state' 17721 'Backbone (1H, 13C, 15N) Chemical Shifts for CBD12 in the free state' 7008 'Resonance assignments for CBD2 in the Ca2+-bound state' 7009 'Resonance assignments for CBD1 in the Ca2+-bound state' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Ca2+ binding alters the interdomain flexibility between the two cytoplasmic calcium-binding domains in the Na+/Ca2+ exchanger.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 21778234 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Salinas Roberto K. . 2 Bruschweiler-Li Lei . . 3 Johnson Eric . . 4 Bruschweiler Rafael . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full 'The Journal of biological chemistry' _Journal_volume 286 _Journal_issue 37 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 32123 _Page_last 32131 _Year 2011 _Details . loop_ _Keyword CBD12 NCX 'Sodium-Calcium Exchanger' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name CBD12 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label CBD12 $CBD12 'Calcium ion' $CA stop_ _System_molecular_weight 32916.169 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function 'Na+ Ca2+ exchanger' stop_ _Database_query_date . _Details 'Monomeric protein' save_ ######################## # Monomeric polymers # ######################## save_CBD12 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common CBD12 _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'Sodium-Calcium Exchanger' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 295 _Mol_residue_sequence ; MSHHHHHHVSKIFFEQGTYQ CLENCGTVALTIIRRGGDLT NTVFVDFRTEDGTANAGSDY EFTEGTVVFKPGETQKEIRV GIIDDDIFEEDENFLVHLSN VKVSSEASEDGILEANHVSA LACLGSPSTATVTIFDDDHA GIFTFEEPVTHVSESIGIME VKVLRTSGARGNVIVPYKTI EGTARGGGEDFEDTCGELEF QNDEIVKTISVKVIDDEEYE KNKTFFLEIGEPRLVEMSEK KGGFTITEEYDDKQPLTSKE EEERRIAEMGRPILGEHTKL EVIIEESYEFKSTVD ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 363 MET 2 364 SER 3 365 HIS 4 366 HIS 5 367 HIS 6 368 HIS 7 369 HIS 8 370 HIS 9 371 VAL 10 372 SER 11 373 LYS 12 374 ILE 13 375 PHE 14 376 PHE 15 377 GLU 16 378 GLN 17 379 GLY 18 380 THR 19 381 TYR 20 382 GLN 21 383 CYS 22 384 LEU 23 385 GLU 24 386 ASN 25 387 CYS 26 388 GLY 27 389 THR 28 390 VAL 29 391 ALA 30 392 LEU 31 393 THR 32 394 ILE 33 395 ILE 34 396 ARG 35 397 ARG 36 398 GLY 37 399 GLY 38 400 ASP 39 401 LEU 40 402 THR 41 403 ASN 42 404 THR 43 405 VAL 44 406 PHE 45 407 VAL 46 408 ASP 47 409 PHE 48 410 ARG 49 411 THR 50 412 GLU 51 413 ASP 52 414 GLY 53 415 THR 54 416 ALA 55 417 ASN 56 418 ALA 57 419 GLY 58 420 SER 59 421 ASP 60 422 TYR 61 423 GLU 62 424 PHE 63 425 THR 64 426 GLU 65 427 GLY 66 428 THR 67 429 VAL 68 430 VAL 69 431 PHE 70 432 LYS 71 433 PRO 72 434 GLY 73 435 GLU 74 436 THR 75 437 GLN 76 438 LYS 77 439 GLU 78 440 ILE 79 441 ARG 80 442 VAL 81 443 GLY 82 444 ILE 83 445 ILE 84 446 ASP 85 447 ASP 86 448 ASP 87 449 ILE 88 450 PHE 89 451 GLU 90 452 GLU 91 453 ASP 92 454 GLU 93 455 ASN 94 456 PHE 95 457 LEU 96 458 VAL 97 459 HIS 98 460 LEU 99 461 SER 100 462 ASN 101 463 VAL 102 464 LYS 103 465 VAL 104 466 SER 105 467 SER 106 468 GLU 107 469 ALA 108 470 SER 109 471 GLU 110 472 ASP 111 473 GLY 112 474 ILE 113 475 LEU 114 476 GLU 115 477 ALA 116 478 ASN 117 479 HIS 118 480 VAL 119 481 SER 120 482 ALA 121 483 LEU 122 484 ALA 123 485 CYS 124 486 LEU 125 487 GLY 126 488 SER 127 489 PRO 128 490 SER 129 491 THR 130 492 ALA 131 493 THR 132 494 VAL 133 495 THR 134 496 ILE 135 497 PHE 136 498 ASP 137 499 ASP 138 500 ASP 139 501 HIS 140 502 ALA 141 503 GLY 142 504 ILE 143 505 PHE 144 506 THR 145 507 PHE 146 508 GLU 147 509 GLU 148 510 PRO 149 511 VAL 150 512 THR 151 513 HIS 152 514 VAL 153 515 SER 154 516 GLU 155 517 SER 156 518 ILE 157 519 GLY 158 520 ILE 159 521 MET 160 522 GLU 161 523 VAL 162 524 LYS 163 525 VAL 164 526 LEU 165 527 ARG 166 528 THR 167 529 SER 168 530 GLY 169 531 ALA 170 532 ARG 171 533 GLY 172 534 ASN 173 535 VAL 174 536 ILE 175 537 VAL 176 538 PRO 177 539 TYR 178 540 LYS 179 541 THR 180 542 ILE 181 543 GLU 182 544 GLY 183 545 THR 184 546 ALA 185 547 ARG 186 548 GLY 187 549 GLY 188 550 GLY 189 551 GLU 190 552 ASP 191 553 PHE 192 554 GLU 193 555 ASP 194 556 THR 195 557 CYS 196 558 GLY 197 559 GLU 198 560 LEU 199 561 GLU 200 562 PHE 201 563 GLN 202 564 ASN 203 565 ASP 204 566 GLU 205 567 ILE 206 568 VAL 207 569 LYS 208 570 THR 209 571 ILE 210 572 SER 211 573 VAL 212 574 LYS 213 575 VAL 214 576 ILE 215 577 ASP 216 578 ASP 217 579 GLU 218 580 GLU 219 581 TYR 220 582 GLU 221 583 LYS 222 584 ASN 223 585 LYS 224 586 THR 225 587 PHE 226 588 PHE 227 589 LEU 228 590 GLU 229 591 ILE 230 592 GLY 231 593 GLU 232 594 PRO 233 595 ARG 234 596 LEU 235 597 VAL 236 598 GLU 237 599 MET 238 600 SER 239 601 GLU 240 602 LYS 241 603 LYS 242 604 GLY 243 605 GLY 244 606 PHE 245 607 THR 246 608 ILE 247 609 THR 248 610 GLU 249 611 GLU 250 612 TYR 251 613 ASP 252 614 ASP 253 615 LYS 254 616 GLN 255 617 PRO 256 618 LEU 257 619 THR 258 620 SER 259 621 LYS 260 622 GLU 261 623 GLU 262 624 GLU 263 625 GLU 264 626 ARG 265 627 ARG 266 628 ILE 267 629 ALA 268 630 GLU 269 631 MET 270 632 GLY 271 633 ARG 272 634 PRO 273 635 ILE 274 636 LEU 275 637 GLY 276 638 GLU 277 639 HIS 278 640 THR 279 641 LYS 280 642 LEU 281 643 GLU 282 644 VAL 283 645 ILE 284 646 ILE 285 647 GLU 286 648 GLU 287 649 SER 288 650 TYR 289 651 GLU 290 652 PHE 291 653 LYS 292 654 SER 293 655 THR 294 656 VAL 295 657 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2013-12-09 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 17721 CBD12 100.00 295 100.00 100.00 0.00e+00 PDB 3US9 "Crystal Structure Of The Ncx1 Intracellular Tandem Calcium Binding Domains(Cbd12)" 100.00 295 99.66 100.00 0.00e+00 stop_ save_ ############# # Ligands # ############# save_CA _Saveframe_category ligand _Mol_type non-polymer _Name_common "CA (CALCIUM ION)" _BMRB_code . _PDB_code CA _Molecular_mass 40.078 _Mol_charge 2 _Mol_paramagnetic No _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Wed Jun 22 17:31:49 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons CA CA CA . 2 . ? stop_ _Mol_thiol_state 'not present' _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Gene_mnemonic _Details $CBD12 dogs 9615 Eukaryota Metazoa Canis familiaris 'Sodium Calcium Exchanger 1' 'AD splice variant' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Variant _Vector_name $CBD12 'recombinant technology' . Escherichia coli BL21 DE3 pET23b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '0.5 mM CBD12 in 20mM Hepes pH 7.0, 80 mM NaCl, 20 mM CaCl2, 20 mM Beta-mercaptoethanol' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $CBD12 0.5 mM '[U-100% 13C; U-100% 15N; U-80% 2H]' H2O 90.0 % 'natural abundance' D2O 10.0 % 'natural abundance' hepes 20.0 mM 'natural abundance' NaCl 80.0 mM 'natural abundance' CaCl2 20.0 mM 'natural abundance' Beta-mercaptoethanol 20.0 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_Analysis_CCPN _Saveframe_category software _Name Analysis_CCPN _Version 2.1.5 loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_HNCA_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_HNCO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HN(CA)CO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)CO' _Sample_label $sample_1 save_ save_3D_HN(CO)CACB_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CACB' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.12 0.1 M pH 7 1 pH pressure 1 . atm temperature 306 2 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 protons ppm 4.642 na indirect . . . 0.251449530 water H 1 protons ppm 4.642 internal direct . . . 1.000000000 water N 15 protons ppm 4.642 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D HNCA' '3D HN(CO)CA' '3D HNCO' '3D HN(CA)CO' '3D HN(CO)CACB' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name CBD12 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 376 14 PHE H H 8.440 . . 2 376 14 PHE C C 176.216 . . 3 376 14 PHE CA C 59.169 . . 4 376 14 PHE N N 117.140 . . 5 377 15 GLU H H 8.734 . . 6 377 15 GLU C C 176.062 . . 7 377 15 GLU CA C 58.663 . . 8 377 15 GLU CB C 29.854 . . 9 377 15 GLU N N 120.166 . . 10 378 16 GLN H H 7.256 . . 11 378 16 GLN C C 173.676 . . 12 378 16 GLN CA C 52.640 . . 13 378 16 GLN N N 110.010 . . 14 379 17 GLY H H 8.276 . . 15 379 17 GLY C C 173.978 . . 16 379 17 GLY CA C 44.622 . . 17 379 17 GLY N N 102.928 . . 18 380 18 THR H H 7.014 . . 19 380 18 THR C C 171.856 . . 20 380 18 THR CA C 58.778 . . 21 380 18 THR CB C 71.015 . . 22 380 18 THR N N 111.221 . . 23 381 19 TYR H H 8.445 . . 24 381 19 TYR C C 174.095 . . 25 381 19 TYR CA C 56.858 . . 26 381 19 TYR N N 121.199 . . 27 382 20 GLN H H 8.483 . . 28 382 20 GLN C C 174.436 . . 29 382 20 GLN CA C 53.636 . . 30 382 20 GLN N N 118.339 . . 31 383 21 CYS H H 8.601 . . 32 383 21 CYS C C 171.169 . . 33 383 21 CYS CA C 55.052 . . 34 383 21 CYS N N 116.238 . . 35 384 22 LEU H H 8.357 . . 36 384 22 LEU C C 179.964 . . 37 384 22 LEU CA C 53.306 . . 38 384 22 LEU N N 121.792 . . 39 385 23 GLU H H 8.090 . . 40 385 23 GLU C C 174.413 . . 41 385 23 GLU CA C 58.296 . . 42 385 23 GLU N N 119.760 . . 43 386 24 ASN H H 7.020 . . 44 386 24 ASN C C 176.973 . . 45 386 24 ASN CA C 51.961 . . 46 386 24 ASN N N 110.871 . . 47 387 25 CYS H H 7.389 . . 48 387 25 CYS C C 175.532 . . 49 387 25 CYS CA C 59.169 . . 50 387 25 CYS N N 115.388 . . 51 388 26 GLY H H 9.117 . . 52 388 26 GLY CA C 46.246 . . 53 388 26 GLY N N 117.392 . . 54 389 27 THR H H 7.667 . . 55 389 27 THR C C 172.258 . . 56 389 27 THR CA C 59.188 . . 57 389 27 THR N N 114.041 . . 58 390 28 VAL H H 8.551 . . 59 390 28 VAL C C 171.603 . . 60 390 28 VAL CA C 58.204 . . 61 390 28 VAL N N 121.641 . . 62 391 29 ALA H H 7.859 . . 63 391 29 ALA N N 129.057 . . 64 397 35 ARG H H 8.749 . . 65 397 35 ARG C C 175.787 . . 66 397 35 ARG CA C 54.725 . . 67 397 35 ARG N N 126.359 . . 68 398 36 GLY H H 8.718 . . 69 398 36 GLY C C 173.214 . . 70 398 36 GLY CA C 43.283 . . 71 398 36 GLY N N 115.783 . . 72 399 37 GLY H H 7.947 . . 73 399 37 GLY C C 172.394 . . 74 399 37 GLY CA C 44.826 . . 75 399 37 GLY N N 105.927 . . 76 400 38 ASP H H 8.298 . . 77 400 38 ASP N N 120.098 . . 78 403 41 ASN H H 6.829 . . 79 403 41 ASN C C 174.570 . . 80 403 41 ASN CA C 52.186 . . 81 403 41 ASN N N 117.617 . . 82 404 42 THR H H 8.261 . . 83 404 42 THR CA C 62.006 . . 84 404 42 THR N N 119.782 . . 85 408 46 ASP H H 8.519 . . 86 408 46 ASP N N 127.973 . . 87 413 51 ASP H H 8.864 . . 88 413 51 ASP C C 177.026 . . 89 413 51 ASP CA C 56.384 . . 90 413 51 ASP N N 124.843 . . 91 414 52 GLY H H 7.280 . . 92 414 52 GLY CA C 45.871 . . 93 414 52 GLY N N 110.617 . . 94 416 54 ALA H H 9.094 . . 95 416 54 ALA CA C 50.012 . . 96 416 54 ALA N N 124.406 . . 97 417 55 ASN H H 9.833 . . 98 417 55 ASN CA C 52.321 . . 99 417 55 ASN N N 117.577 . . 100 418 56 ALA H H 9.876 . . 101 418 56 ALA C C 179.110 . . 102 418 56 ALA CA C 51.501 . . 103 418 56 ALA N N 134.034 . . 104 419 57 GLY H H 8.575 . . 105 419 57 GLY C C 173.288 . . 106 419 57 GLY CA C 44.777 . . 107 419 57 GLY N N 112.439 . . 108 420 58 SER H H 7.145 . . 109 420 58 SER CA C 58.632 . . 110 420 58 SER N N 112.452 . . 111 421 59 ASP H H 8.861 . . 112 421 59 ASP C C 175.798 . . 113 421 59 ASP CA C 54.410 . . 114 421 59 ASP N N 117.506 . . 115 422 60 TYR H H 7.175 . . 116 422 60 TYR C C 170.930 . . 117 422 60 TYR CA C 55.375 . . 118 422 60 TYR N N 117.468 . . 119 423 61 GLU H H 8.535 . . 120 423 61 GLU C C 175.457 . . 121 423 61 GLU CA C 54.346 . . 122 423 61 GLU N N 122.979 . . 123 424 62 PHE H H 8.809 . . 124 424 62 PHE CA C 59.473 . . 125 424 62 PHE N N 128.497 . . 126 425 63 THR H H 7.515 . . 127 425 63 THR C C 169.653 . . 128 425 63 THR CA C 61.667 . . 129 425 63 THR N N 125.231 . . 130 426 64 GLU H H 7.708 . . 131 426 64 GLU C C 174.388 . . 132 426 64 GLU CA C 53.075 . . 133 426 64 GLU N N 122.359 . . 134 427 65 GLY H H 7.033 . . 135 427 65 GLY C C 170.997 . . 136 427 65 GLY CA C 45.075 . . 137 427 65 GLY N N 108.164 . . 138 428 66 THR H H 8.179 . . 139 428 66 THR N N 117.707 . . 140 432 70 LYS H H 8.685 . . 141 432 70 LYS N N 123.706 . . 142 434 72 GLY H H 8.828 . . 143 434 72 GLY C C 174.389 . . 144 434 72 GLY CA C 44.885 . . 145 434 72 GLY N N 112.082 . . 146 435 73 GLU H H 8.195 . . 147 435 73 GLU N N 119.409 . . 148 437 75 GLN H H 7.670 . . 149 437 75 GLN C C 174.293 . . 150 437 75 GLN CA C 54.660 . . 151 437 75 GLN N N 119.443 . . 152 438 76 LYS H H 8.940 . . 153 438 76 LYS N N 122.908 . . 154 441 79 ARG H H 7.851 . . 155 441 79 ARG N N 119.716 . . 156 443 81 GLY H H 9.195 . . 157 443 81 GLY C C 171.614 . . 158 443 81 GLY N N 115.133 . . 159 444 82 ILE H H 8.107 . . 160 444 82 ILE C C 176.874 . . 161 444 82 ILE CA C 57.299 . . 162 444 82 ILE N N 127.150 . . 163 445 83 ILE H H 7.430 . . 164 445 83 ILE C C 172.700 . . 165 445 83 ILE CA C 61.043 . . 166 445 83 ILE N N 131.177 . . 167 446 84 ASP H H 8.479 . . 168 446 84 ASP C C 175.499 . . 169 446 84 ASP CA C 52.176 . . 170 446 84 ASP N N 126.347 . . 171 447 85 ASP H H 7.410 . . 172 447 85 ASP CA C 52.134 . . 173 447 85 ASP N N 122.811 . . 174 448 86 ASP H H 8.285 . . 175 448 86 ASP CA C 52.898 . . 176 448 86 ASP N N 118.577 . . 177 449 87 ILE H H 7.702 . . 178 449 87 ILE CA C 59.705 . . 179 449 87 ILE N N 122.705 . . 180 450 88 PHE H H 9.022 . . 181 450 88 PHE C C 172.738 . . 182 450 88 PHE N N 132.285 . . 183 451 89 GLU H H 6.322 . . 184 451 89 GLU C C 173.760 . . 185 451 89 GLU CA C 53.549 . . 186 451 89 GLU N N 127.537 . . 187 452 90 GLU H H 7.822 . . 188 452 90 GLU C C 175.602 . . 189 452 90 GLU CA C 55.163 . . 190 452 90 GLU N N 115.580 . . 191 453 91 ASP H H 8.177 . . 192 453 91 ASP C C 175.622 . . 193 453 91 ASP CA C 55.565 . . 194 453 91 ASP N N 120.219 . . 195 454 92 GLU H H 7.370 . . 196 454 92 GLU C C 174.654 . . 197 454 92 GLU CA C 54.032 . . 198 454 92 GLU N N 120.934 . . 199 455 93 ASN H H 8.118 . . 200 455 93 ASN N N 114.534 . . 201 459 97 HIS H H 8.936 . . 202 459 97 HIS C C 177.023 . . 203 459 97 HIS CA C 54.730 . . 204 459 97 HIS N N 124.075 . . 205 460 98 LEU H H 8.717 . . 206 460 98 LEU C C 176.747 . . 207 460 98 LEU CA C 52.858 . . 208 460 98 LEU N N 122.613 . . 209 461 99 SER H H 8.826 . . 210 461 99 SER C C 172.509 . . 211 461 99 SER CA C 57.713 . . 212 461 99 SER N N 112.364 . . 213 462 100 ASN H H 8.554 . . 214 462 100 ASN C C 173.297 . . 215 462 100 ASN CA C 53.154 . . 216 462 100 ASN N N 116.149 . . 217 463 101 VAL H H 7.936 . . 218 463 101 VAL C C 177.353 . . 219 463 101 VAL CA C 62.525 . . 220 463 101 VAL N N 118.448 . . 221 464 102 LYS H H 9.138 . . 222 464 102 LYS C C 174.428 . . 223 464 102 LYS CA C 54.436 . . 224 464 102 LYS N N 129.683 . . 225 465 103 VAL H H 8.553 . . 226 465 103 VAL C C 175.636 . . 227 465 103 VAL CA C 60.127 . . 228 465 103 VAL N N 120.334 . . 229 466 104 SER H H 8.755 . . 230 466 104 SER C C 173.982 . . 231 466 104 SER CA C 57.536 . . 232 466 104 SER N N 121.057 . . 233 467 105 SER H H 8.474 . . 234 467 105 SER C C 174.451 . . 235 467 105 SER CA C 57.362 . . 236 467 105 SER N N 120.078 . . 237 468 106 GLU H H 8.372 . . 238 468 106 GLU C C 176.179 . . 239 468 106 GLU CA C 56.322 . . 240 468 106 GLU N N 123.391 . . 241 469 107 ALA H H 8.196 . . 242 469 107 ALA C C 177.637 . . 243 469 107 ALA CA C 52.029 . . 244 469 107 ALA N N 124.511 . . 245 470 108 SER H H 8.029 . . 246 470 108 SER C C 175.161 . . 247 470 108 SER CA C 57.794 . . 248 470 108 SER N N 115.313 . . 249 471 109 GLU H H 8.505 . . 250 471 109 GLU CA C 56.901 . . 251 471 109 GLU N N 123.401 . . 252 472 110 ASP H H 8.091 . . 253 472 110 ASP C C 176.636 . . 254 472 110 ASP CA C 54.125 . . 255 472 110 ASP N N 119.623 . . 256 473 111 GLY H H 7.991 . . 257 473 111 GLY C C 174.130 . . 258 473 111 GLY CA C 45.103 . . 259 473 111 GLY N N 108.985 . . 260 474 112 ILE H H 7.723 . . 261 474 112 ILE C C 176.194 . . 262 474 112 ILE CA C 60.720 . . 263 474 112 ILE N N 120.673 . . 264 475 113 LEU H H 8.174 . . 265 475 113 LEU CA C 54.720 . . 266 475 113 LEU N N 126.278 . . 267 476 114 GLU H H 8.203 . . 268 476 114 GLU C C 176.100 . . 269 476 114 GLU CA C 55.935 . . 270 476 114 GLU N N 122.179 . . 271 477 115 ALA H H 8.133 . . 272 477 115 ALA C C 177.373 . . 273 477 115 ALA CA C 52.383 . . 274 477 115 ALA N N 124.819 . . 275 478 116 ASN H H 8.172 . . 276 478 116 ASN N N 117.061 . . 277 480 118 VAL H H 7.589 . . 278 480 118 VAL CA C 60.709 . . 279 480 118 VAL N N 121.052 . . 280 484 122 ALA H H 7.685 . . 281 484 122 ALA C C 174.578 . . 282 484 122 ALA CA C 50.670 . . 283 484 122 ALA N N 121.699 . . 284 485 123 CYS H H 8.740 . . 285 485 123 CYS C C 172.388 . . 286 485 123 CYS CA C 55.332 . . 287 485 123 CYS N N 115.858 . . 288 486 124 LEU H H 8.504 . . 289 486 124 LEU C C 178.517 . . 290 486 124 LEU CA C 55.136 . . 291 486 124 LEU N N 120.595 . . 292 487 125 GLY H H 9.013 . . 293 487 125 GLY C C 171.462 . . 294 487 125 GLY CA C 43.111 . . 295 487 125 GLY N N 112.092 . . 296 488 126 SER H H 7.664 . . 297 488 126 SER N N 114.739 . . 298 490 128 SER H H 9.270 . . 299 490 128 SER CA C 57.917 . . 300 490 128 SER N N 117.837 . . 301 491 129 THR H H 7.708 . . 302 491 129 THR N N 120.321 . . 303 497 135 PHE H H 9.636 . . 304 497 135 PHE CA C 55.229 . . 305 497 135 PHE N N 131.516 . . 306 498 136 ASP H H 9.093 . . 307 498 136 ASP C C 175.813 . . 308 498 136 ASP N N 126.516 . . 309 499 137 ASP H H 7.767 . . 310 499 137 ASP C C 176.963 . . 311 499 137 ASP CA C 52.718 . . 312 499 137 ASP N N 120.904 . . 313 500 138 ASP H H 8.214 . . 314 500 138 ASP C C 174.518 . . 315 500 138 ASP CA C 54.342 . . 316 500 138 ASP N N 117.987 . . 317 501 139 HIS H H 8.478 . . 318 501 139 HIS C C 175.384 . . 319 501 139 HIS CA C 59.311 . . 320 501 139 HIS N N 124.255 . . 321 502 140 ALA H H 7.568 . . 322 502 140 ALA C C 178.915 . . 323 502 140 ALA CA C 53.072 . . 324 502 140 ALA N N 127.510 . . 325 503 141 GLY H H 11.693 . . 326 503 141 GLY C C 171.864 . . 327 503 141 GLY CA C 43.973 . . 328 503 141 GLY N N 115.412 . . 329 504 142 ILE H H 8.081 . . 330 504 142 ILE C C 178.199 . . 331 504 142 ILE CA C 60.106 . . 332 504 142 ILE N N 121.283 . . 333 505 143 PHE H H 8.794 . . 334 505 143 PHE C C 175.075 . . 335 505 143 PHE CA C 57.449 . . 336 505 143 PHE N N 128.082 . . 337 506 144 THR H H 8.678 . . 338 506 144 THR C C 172.213 . . 339 506 144 THR CA C 59.325 . . 340 506 144 THR N N 117.302 . . 341 507 145 PHE H H 8.876 . . 342 507 145 PHE CA C 59.082 . . 343 507 145 PHE N N 123.872 . . 344 508 146 GLU H H 10.009 . . 345 508 146 GLU C C 175.599 . . 346 508 146 GLU CA C 59.087 . . 347 508 146 GLU N N 122.014 . . 348 509 147 GLU H H 7.355 . . 349 509 147 GLU N N 112.772 . . 350 511 149 VAL H H 6.669 . . 351 511 149 VAL C C 174.014 . . 352 511 149 VAL CA C 59.212 . . 353 511 149 VAL N N 120.006 . . 354 512 150 THR H H 8.605 . . 355 512 150 THR C C 170.432 . . 356 512 150 THR CA C 60.309 . . 357 512 150 THR N N 121.634 . . 358 513 151 HIS H H 7.892 . . 359 513 151 HIS C C 174.572 . . 360 513 151 HIS CA C 53.900 . . 361 513 151 HIS N N 123.417 . . 362 514 152 VAL H H 8.973 . . 363 514 152 VAL C C 173.529 . . 364 514 152 VAL CA C 58.106 . . 365 514 152 VAL N N 118.098 . . 366 515 153 SER H H 8.221 . . 367 515 153 SER C C 176.009 . . 368 515 153 SER CA C 57.545 . . 369 515 153 SER N N 116.731 . . 370 516 154 GLU H H 8.392 . . 371 516 154 GLU CA C 57.650 . . 372 516 154 GLU N N 128.527 . . 373 517 155 SER H H 7.878 . . 374 517 155 SER CA C 56.858 . . 375 517 155 SER N N 109.477 . . 376 518 156 ILE H H 7.558 . . 377 518 156 ILE C C 174.746 . . 378 518 156 ILE CA C 62.293 . . 379 518 156 ILE N N 121.867 . . 380 519 157 GLY H H 8.632 . . 381 519 157 GLY C C 172.591 . . 382 519 157 GLY CA C 45.607 . . 383 519 157 GLY N N 113.219 . . 384 520 158 ILE H H 7.683 . . 385 520 158 ILE C C 174.867 . . 386 520 158 ILE CA C 59.502 . . 387 520 158 ILE N N 125.475 . . 388 521 159 MET H H 8.992 . . 389 521 159 MET C C 173.516 . . 390 521 159 MET CA C 53.538 . . 391 521 159 MET N N 127.612 . . 392 522 160 GLU H H 8.242 . . 393 522 160 GLU C C 175.895 . . 394 522 160 GLU CA C 54.443 . . 395 522 160 GLU N N 123.534 . . 396 523 161 VAL H H 8.893 . . 397 523 161 VAL C C 174.654 . . 398 523 161 VAL CA C 59.768 . . 399 523 161 VAL N N 123.189 . . 400 524 162 LYS H H 8.529 . . 401 524 162 LYS C C 175.787 . . 402 524 162 LYS CA C 56.301 . . 403 524 162 LYS N N 124.221 . . 404 525 163 VAL H H 8.757 . . 405 525 163 VAL C C 174.112 . . 406 525 163 VAL CA C 62.302 . . 407 525 163 VAL N N 122.087 . . 408 526 164 LEU H H 8.704 . . 409 526 164 LEU CA C 53.661 . . 410 526 164 LEU N N 125.345 . . 411 527 165 ARG H H 7.861 . . 412 527 165 ARG N N 123.662 . . 413 528 166 THR H H 8.492 . . 414 528 166 THR CA C 59.765 . . 415 528 166 THR N N 118.165 . . 416 529 167 SER H H 8.539 . . 417 529 167 SER CA C 62.228 . . 418 529 167 SER N N 112.768 . . 419 530 168 GLY H H 7.624 . . 420 530 168 GLY C C 171.056 . . 421 530 168 GLY CA C 45.079 . . 422 530 168 GLY N N 109.692 . . 423 531 169 ALA H H 8.447 . . 424 531 169 ALA N N 123.056 . . 425 533 171 GLY H H 9.037 . . 426 533 171 GLY C C 172.291 . . 427 533 171 GLY CA C 42.907 . . 428 533 171 GLY N N 115.727 . . 429 534 172 ASN H H 8.384 . . 430 534 172 ASN C C 174.080 . . 431 534 172 ASN CA C 51.488 . . 432 534 172 ASN N N 119.597 . . 433 535 173 VAL H H 8.932 . . 434 535 173 VAL CA C 59.978 . . 435 535 173 VAL N N 124.634 . . 436 536 174 ILE H H 8.806 . . 437 536 174 ILE CA C 58.384 . . 438 536 174 ILE N N 126.848 . . 439 537 175 VAL H H 8.918 . . 440 537 175 VAL N N 126.777 . . 441 539 177 TYR H H 8.288 . . 442 539 177 TYR N N 115.419 . . 443 540 178 LYS H H 8.598 . . 444 540 178 LYS C C 174.884 . . 445 540 178 LYS CA C 55.510 . . 446 540 178 LYS N N 115.586 . . 447 541 179 THR H H 7.876 . . 448 541 179 THR C C 175.374 . . 449 541 179 THR CA C 59.778 . . 450 541 179 THR N N 110.448 . . 451 542 180 ILE H H 9.068 . . 452 542 180 ILE C C 176.053 . . 453 542 180 ILE CA C 60.483 . . 454 542 180 ILE N N 121.292 . . 455 543 181 GLU H H 8.884 . . 456 543 181 GLU C C 176.377 . . 457 543 181 GLU CA C 58.149 . . 458 543 181 GLU N N 125.424 . . 459 544 182 GLY H H 7.420 . . 460 544 182 GLY C C 174.709 . . 461 544 182 GLY CA C 45.870 . . 462 544 182 GLY N N 111.377 . . 463 545 183 THR H H 8.298 . . 464 545 183 THR C C 176.526 . . 465 545 183 THR CA C 62.617 . . 466 545 183 THR N N 119.860 . . 467 546 184 ALA H H 9.201 . . 468 546 184 ALA C C 175.830 . . 469 546 184 ALA CA C 50.695 . . 470 546 184 ALA N N 124.202 . . 471 547 185 ARG H H 9.897 . . 472 547 185 ARG C C 176.561 . . 473 547 185 ARG CA C 53.962 . . 474 547 185 ARG N N 123.140 . . 475 548 186 GLY H H 9.164 . . 476 548 186 GLY C C 177.099 . . 477 548 186 GLY CA C 42.287 . . 478 548 186 GLY N N 113.167 . . 479 549 187 GLY H H 9.018 . . 480 549 187 GLY C C 175.445 . . 481 549 187 GLY CA C 46.363 . . 482 549 187 GLY N N 111.940 . . 483 550 188 GLY H H 7.709 . . 484 550 188 GLY C C 173.184 . . 485 550 188 GLY CA C 44.203 . . 486 550 188 GLY N N 104.643 . . 487 551 189 GLU H H 7.048 . . 488 551 189 GLU C C 174.714 . . 489 551 189 GLU CA C 57.895 . . 490 551 189 GLU N N 121.526 . . 491 552 190 ASP H H 8.387 . . 492 552 190 ASP CA C 54.897 . . 493 552 190 ASP N N 114.327 . . 494 553 191 PHE H H 7.603 . . 495 553 191 PHE C C 172.630 . . 496 553 191 PHE CA C 56.107 . . 497 553 191 PHE N N 117.785 . . 498 554 192 GLU H H 7.977 . . 499 554 192 GLU CA C 54.555 . . 500 554 192 GLU N N 120.120 . . 501 555 193 ASP H H 8.199 . . 502 555 193 ASP C C 176.171 . . 503 555 193 ASP CA C 54.713 . . 504 555 193 ASP N N 125.747 . . 505 556 194 THR H H 8.514 . . 506 556 194 THR C C 171.201 . . 507 556 194 THR CA C 61.566 . . 508 556 194 THR N N 118.803 . . 509 557 195 CYS H H 8.017 . . 510 557 195 CYS C C 172.725 . . 511 557 195 CYS CA C 55.603 . . 512 557 195 CYS N N 121.814 . . 513 558 196 GLY H H 7.062 . . 514 558 196 GLY C C 171.273 . . 515 558 196 GLY N N 109.055 . . 516 559 197 GLU H H 8.420 . . 517 559 197 GLU C C 175.487 . . 518 559 197 GLU CA C 54.664 . . 519 559 197 GLU N N 120.214 . . 520 560 198 LEU H H 9.070 . . 521 560 198 LEU C C 175.426 . . 522 560 198 LEU CA C 52.982 . . 523 560 198 LEU N N 125.517 . . 524 561 199 GLU H H 8.565 . . 525 561 199 GLU C C 175.076 . . 526 561 199 GLU CA C 54.632 . . 527 561 199 GLU N N 124.345 . . 528 562 200 PHE H H 9.513 . . 529 562 200 PHE CA C 56.350 . . 530 562 200 PHE N N 127.230 . . 531 563 201 GLN H H 9.380 . . 532 563 201 GLN N N 121.904 . . 533 565 203 ASP H H 9.734 . . 534 565 203 ASP C C 174.706 . . 535 565 203 ASP CA C 57.302 . . 536 565 203 ASP N N 120.694 . . 537 566 204 GLU H H 8.119 . . 538 566 204 GLU C C 177.793 . . 539 566 204 GLU CA C 56.520 . . 540 566 204 GLU N N 122.027 . . 541 567 205 ILE H H 8.510 . . 542 567 205 ILE C C 174.469 . . 543 567 205 ILE CA C 61.876 . . 544 567 205 ILE N N 115.909 . . 545 568 206 VAL H H 7.354 . . 546 568 206 VAL C C 173.397 . . 547 568 206 VAL CA C 60.598 . . 548 568 206 VAL N N 119.627 . . 549 569 207 LYS H H 8.120 . . 550 569 207 LYS C C 174.672 . . 551 569 207 LYS CA C 53.345 . . 552 569 207 LYS N N 124.989 . . 553 570 208 THR H H 7.767 . . 554 570 208 THR C C 173.844 . . 555 570 208 THR CA C 59.624 . . 556 570 208 THR N N 110.878 . . 557 571 209 ILE H H 8.796 . . 558 571 209 ILE N N 123.482 . . 559 573 211 VAL H H 8.613 . . 560 573 211 VAL C C 174.596 . . 561 573 211 VAL CA C 60.173 . . 562 573 211 VAL N N 123.459 . . 563 574 212 LYS H H 9.054 . . 564 574 212 LYS C C 174.092 . . 565 574 212 LYS CA C 56.477 . . 566 574 212 LYS N N 129.291 . . 567 575 213 VAL H H 8.139 . . 568 575 213 VAL C C 175.990 . . 569 575 213 VAL CA C 61.787 . . 570 575 213 VAL N N 128.824 . . 571 576 214 ILE H H 7.870 . . 572 576 214 ILE C C 175.247 . . 573 576 214 ILE CA C 59.369 . . 574 576 214 ILE N N 130.927 . . 575 577 215 ASP H H 8.716 . . 576 577 215 ASP CA C 52.864 . . 577 577 215 ASP N N 131.466 . . 578 578 216 ASP H H 8.110 . . 579 578 216 ASP C C 177.206 . . 580 578 216 ASP N N 120.958 . . 581 579 217 GLU H H 8.324 . . 582 579 217 GLU CA C 54.452 . . 583 579 217 GLU N N 119.375 . . 584 580 218 GLU H H 7.714 . . 585 580 218 GLU C C 174.384 . . 586 580 218 GLU CA C 54.008 . . 587 580 218 GLU N N 119.113 . . 588 581 219 TYR H H 8.780 . . 589 581 219 TYR C C 174.003 . . 590 581 219 TYR CA C 57.533 . . 591 581 219 TYR N N 127.307 . . 592 582 220 GLU H H 7.980 . . 593 582 220 GLU C C 174.618 . . 594 582 220 GLU CA C 53.992 . . 595 582 220 GLU N N 129.524 . . 596 583 221 LYS H H 7.667 . . 597 583 221 LYS C C 176.319 . . 598 583 221 LYS CA C 55.848 . . 599 583 221 LYS N N 117.859 . . 600 584 222 ASN H H 8.267 . . 601 584 222 ASN CA C 53.683 . . 602 584 222 ASN N N 120.563 . . 603 585 223 LYS H H 7.996 . . 604 585 223 LYS C C 174.614 . . 605 585 223 LYS CA C 54.963 . . 606 585 223 LYS N N 122.688 . . 607 586 224 THR H H 7.950 . . 608 586 224 THR CA C 59.352 . . 609 586 224 THR N N 111.572 . . 610 587 225 PHE H H 8.444 . . 611 587 225 PHE C C 169.976 . . 612 587 225 PHE N N 121.327 . . 613 588 226 PHE H H 8.974 . . 614 588 226 PHE C C 174.912 . . 615 588 226 PHE CA C 54.853 . . 616 588 226 PHE N N 114.871 . . 617 589 227 LEU H H 8.645 . . 618 589 227 LEU C C 174.358 . . 619 589 227 LEU CA C 53.379 . . 620 589 227 LEU N N 121.912 . . 621 590 228 GLU H H 9.059 . . 622 590 228 GLU C C 176.790 . . 623 590 228 GLU CA C 54.449 . . 624 590 228 GLU N N 123.776 . . 625 591 229 ILE H H 8.255 . . 626 591 229 ILE C C 175.029 . . 627 591 229 ILE CA C 59.063 . . 628 591 229 ILE N N 119.007 . . 629 592 230 GLY H H 8.108 . . 630 592 230 GLY C C 171.548 . . 631 592 230 GLY CA C 42.058 . . 632 592 230 GLY N N 108.938 . . 633 593 231 GLU H H 7.653 . . 634 593 231 GLU N N 115.796 . . 635 595 233 ARG H H 8.599 . . 636 595 233 ARG C C 174.432 . . 637 595 233 ARG CA C 53.770 . . 638 595 233 ARG N N 117.682 . . 639 596 234 LEU H H 8.576 . . 640 596 234 LEU C C 177.063 . . 641 596 234 LEU CA C 54.483 . . 642 596 234 LEU N N 125.682 . . 643 597 235 VAL H H 8.412 . . 644 597 235 VAL C C 175.744 . . 645 597 235 VAL CA C 62.379 . . 646 597 235 VAL N N 126.220 . . 647 598 236 GLU H H 8.378 . . 648 598 236 GLU CA C 55.770 . . 649 598 236 GLU N N 126.208 . . 650 599 237 MET H H 8.414 . . 651 599 237 MET C C 176.335 . . 652 599 237 MET CA C 55.094 . . 653 599 237 MET N N 122.906 . . 654 600 238 SER H H 8.209 . . 655 600 238 SER C C 174.595 . . 656 600 238 SER CA C 58.328 . . 657 600 238 SER N N 117.246 . . 658 601 239 GLU H H 8.326 . . 659 601 239 GLU C C 176.405 . . 660 601 239 GLU CA C 56.344 . . 661 601 239 GLU N N 122.937 . . 662 602 240 LYS H H 8.075 . . 663 602 240 LYS C C 176.531 . . 664 602 240 LYS N N 122.288 . . 665 603 241 LYS H H 8.167 . . 666 603 241 LYS C C 177.012 . . 667 603 241 LYS CA C 55.861 . . 668 603 241 LYS N N 122.865 . . 669 604 242 GLY H H 8.243 . . 670 604 242 GLY C C 174.383 . . 671 604 242 GLY CA C 44.789 . . 672 604 242 GLY N N 110.416 . . 673 605 243 GLY H H 8.053 . . 674 605 243 GLY C C 173.725 . . 675 605 243 GLY CA C 44.670 . . 676 605 243 GLY N N 108.836 . . 677 606 244 PHE H H 7.919 . . 678 606 244 PHE C C 175.668 . . 679 606 244 PHE CA C 57.232 . . 680 606 244 PHE N N 120.132 . . 681 607 245 THR H H 8.009 . . 682 607 245 THR C C 174.010 . . 683 607 245 THR CA C 61.337 . . 684 607 245 THR N N 117.164 . . 685 608 246 ILE H H 8.097 . . 686 608 246 ILE C C 176.217 . . 687 608 246 ILE CA C 60.836 . . 688 608 246 ILE N N 123.816 . . 689 609 247 THR H H 7.990 . . 690 609 247 THR C C 174.357 . . 691 609 247 THR CA C 61.313 . . 692 609 247 THR N N 118.257 . . 693 610 248 GLU H H 8.192 . . 694 610 248 GLU C C 176.084 . . 695 610 248 GLU CA C 56.124 . . 696 610 248 GLU N N 123.444 . . 697 611 249 GLU H H 8.162 . . 698 611 249 GLU CA C 56.042 . . 699 611 249 GLU N N 121.720 . . 700 612 250 TYR H H 7.987 . . 701 612 250 TYR C C 175.331 . . 702 612 250 TYR CA C 57.430 . . 703 612 250 TYR N N 121.381 . . 704 613 251 ASP H H 8.057 . . 705 613 251 ASP C C 175.675 . . 706 613 251 ASP CA C 53.657 . . 707 613 251 ASP N N 122.892 . . 708 614 252 ASP H H 8.039 . . 709 614 252 ASP C C 176.187 . . 710 614 252 ASP CA C 53.911 . . 711 614 252 ASP N N 121.617 . . 712 615 253 LYS H H 8.017 . . 713 615 253 LYS C C 176.482 . . 714 615 253 LYS CA C 55.742 . . 715 615 253 LYS N N 120.548 . . 716 616 254 GLN H H 7.967 . . 717 616 254 GLN N N 122.156 . . 718 621 259 LYS H H 8.038 . . 719 621 259 LYS C C 178.271 . . 720 621 259 LYS CA C 58.191 . . 721 621 259 LYS N N 123.187 . . 722 622 260 GLU H H 7.724 . . 723 622 260 GLU C C 179.448 . . 724 622 260 GLU CA C 58.457 . . 725 622 260 GLU N N 119.802 . . 726 623 261 GLU H H 8.325 . . 727 623 261 GLU C C 179.080 . . 728 623 261 GLU CA C 58.973 . . 729 623 261 GLU N N 120.680 . . 730 624 262 GLU H H 7.904 . . 731 624 262 GLU C C 178.365 . . 732 624 262 GLU CA C 58.375 . . 733 624 262 GLU N N 121.609 . . 734 625 263 GLU H H 8.146 . . 735 625 263 GLU C C 180.055 . . 736 625 263 GLU CA C 58.997 . . 737 625 263 GLU N N 119.082 . . 738 626 264 ARG H H 7.936 . . 739 626 264 ARG C C 177.968 . . 740 626 264 ARG CA C 58.473 . . 741 626 264 ARG N N 120.736 . . 742 627 265 ARG H H 7.859 . . 743 627 265 ARG C C 179.560 . . 744 627 265 ARG CA C 58.870 . . 745 627 265 ARG N N 121.559 . . 746 628 266 ILE H H 8.126 . . 747 628 266 ILE C C 178.345 . . 748 628 266 ILE CA C 63.360 . . 749 628 266 ILE N N 120.026 . . 750 629 267 ALA H H 7.982 . . 751 629 267 ALA C C 181.835 . . 752 629 267 ALA CA C 54.614 . . 753 629 267 ALA N N 121.839 . . 754 630 268 GLU H H 7.649 . . 755 630 268 GLU C C 178.603 . . 756 630 268 GLU CA C 58.028 . . 757 630 268 GLU N N 117.815 . . 758 631 269 MET H H 7.869 . . 759 631 269 MET C C 176.432 . . 760 631 269 MET CA C 56.680 . . 761 631 269 MET N N 118.115 . . 762 632 270 GLY H H 7.349 . . 763 632 270 GLY C C 172.714 . . 764 632 270 GLY CA C 43.535 . . 765 632 270 GLY N N 108.697 . . 766 633 271 ARG H H 6.396 . . 767 633 271 ARG CA C 54.186 . . 768 633 271 ARG N N 119.880 . . 769 635 273 ILE H H 8.383 . . 770 635 273 ILE CA C 59.160 . . 771 635 273 ILE N N 113.998 . . 772 636 274 LEU H H 8.443 . . 773 636 274 LEU C C 177.934 . . 774 636 274 LEU CA C 54.145 . . 775 636 274 LEU N N 120.739 . . 776 637 275 GLY H H 8.864 . . 777 637 275 GLY C C 173.522 . . 778 637 275 GLY CA C 43.462 . . 779 637 275 GLY N N 109.835 . . 780 638 276 GLU H H 8.205 . . 781 638 276 GLU C C 176.770 . . 782 638 276 GLU CA C 59.285 . . 783 638 276 GLU N N 121.173 . . 784 639 277 HIS H H 8.027 . . 785 639 277 HIS C C 174.436 . . 786 639 277 HIS CA C 53.099 . . 787 639 277 HIS N N 115.559 . . 788 640 278 THR H H 8.390 . . 789 640 278 THR C C 172.757 . . 790 640 278 THR CA C 53.115 . . 791 640 278 THR N N 113.922 . . 792 641 279 LYS H H 7.898 . . 793 641 279 LYS C C 173.659 . . 794 641 279 LYS CA C 55.079 . . 795 641 279 LYS N N 123.759 . . 796 642 280 LEU H H 9.053 . . 797 642 280 LEU C C 174.498 . . 798 642 280 LEU CA C 52.812 . . 799 642 280 LEU N N 131.045 . . 800 643 281 GLU H H 8.096 . . 801 643 281 GLU C C 172.578 . . 802 643 281 GLU CA C 54.813 . . 803 643 281 GLU N N 129.537 . . 804 644 282 VAL H H 8.431 . . 805 644 282 VAL C C 173.860 . . 806 644 282 VAL CA C 60.245 . . 807 644 282 VAL N N 128.453 . . 808 645 283 ILE H H 8.073 . . 809 645 283 ILE C C 174.784 . . 810 645 283 ILE N N 126.620 . . 811 646 284 ILE H H 8.892 . . 812 646 284 ILE C C 177.131 . . 813 646 284 ILE CA C 59.981 . . 814 646 284 ILE N N 127.729 . . 815 647 285 GLU H H 9.337 . . 816 647 285 GLU C C 175.750 . . 817 647 285 GLU CA C 55.195 . . 818 647 285 GLU N N 128.883 . . 819 648 286 GLU H H 8.021 . . 820 648 286 GLU N N 128.220 . . 821 651 289 GLU H H 7.784 . . 822 651 289 GLU CA C 55.866 . . 823 651 289 GLU N N 121.950 . . 824 652 290 PHE H H 7.788 . . 825 652 290 PHE C C 175.617 . . 826 652 290 PHE CA C 57.551 . . 827 652 290 PHE N N 121.562 . . 828 653 291 LYS H H 7.728 . . 829 653 291 LYS N N 123.295 . . 830 655 293 THR H H 7.956 . . 831 655 293 THR N N 116.119 . . 832 656 294 VAL H H 7.915 . . 833 656 294 VAL C C 174.936 . . 834 656 294 VAL CA C 61.588 . . 835 656 294 VAL N N 121.549 . . 836 657 295 ASP H H 7.779 . . 837 657 295 ASP N N 129.319 . . stop_ save_