data_18959 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR structure of Rsa1p238-259 from S. Cerevisiae ; _BMRB_accession_number 18959 _BMRB_flat_file_name bmr18959.str _Entry_type original _Submission_date 2013-01-17 _Accession_date 2013-01-17 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Quinternet Marc . . 2 Manival Xavier . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 312 "13C chemical shifts" 90 "15N chemical shifts" 26 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-02-17 update BMRB 'update entry citation' 2013-11-26 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Characterization of the interaction between protein Snu13p/15.5K and the Rsa1p/NUFIP factor and demonstration of its functional importance for snoRNP assembly.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 24234454 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Rothe Benjamin . . 2 Back Regis . . 3 Quinternet Marc . . 4 Bizarro Jonathan . . 5 Robert Marie-Cecile . . 6 Blaud Magali . . 7 Romier Christophe . . 8 Manival Xavier . . 9 Charpentier Bruno . . 10 Bertrand Edouard . . 11 Branlant Christiane . . stop_ _Journal_abbreviation 'Nucleic Acids Res.' _Journal_name_full 'Nucleic acids research' _Journal_volume 42 _Journal_issue 3 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2015 _Page_last 2036 _Year 2014 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name Rsa1p238-259 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Rsa1p238-259 $Rsa1p238-259 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Rsa1p238-259 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Rsa1p238-259 _Molecular_mass 2840.349 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 22 _Mol_residue_sequence ; TDEDVKKWREERKKMWLLKI SN ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 THR 2 2 ASP 3 3 GLU 4 4 ASP 5 5 VAL 6 6 LYS 7 7 LYS 8 8 TRP 9 9 ARG 10 10 GLU 11 11 GLU 12 12 ARG 13 13 LYS 14 14 LYS 15 15 MET 16 16 TRP 17 17 LEU 18 18 LEU 19 19 LYS 20 20 ILE 21 21 SER 22 22 ASN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-08-19 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2M3F "Nmr Structure Of Rsa1p238-259 From S. Cerevisiae" 100.00 22 100.00 100.00 8.84e-05 PDB 4NUT "Crystal Structure Of The Complex Between Snu13p And The Pep Domain Of Rsa1" 100.00 57 100.00 100.00 3.44e-05 DBJ GAA26783 "K7_Rsa1p [Saccharomyces cerevisiae Kyokai no. 7]" 100.00 381 100.00 100.00 9.35e-04 EMBL CAA97906 "unnamed protein product [Saccharomyces cerevisiae]" 100.00 381 100.00 100.00 6.28e-04 EMBL CAY86767 "Rsa1p [Saccharomyces cerevisiae EC1118]" 100.00 381 100.00 100.00 6.28e-04 GB AHY77988 "Rsa1p [Saccharomyces cerevisiae YJM993]" 100.00 381 100.00 100.00 6.28e-04 GB AJP41956 "Rsa1p [Saccharomyces cerevisiae YJM1078]" 100.00 381 100.00 100.00 6.28e-04 GB AJU23400 "Rsa1p [Saccharomyces cerevisiae YJM1526]" 100.00 381 100.00 100.00 6.28e-04 GB AJU24088 "Rsa1p [Saccharomyces cerevisiae YJM1549]" 100.00 381 100.00 100.00 5.37e-04 GB AJU24761 "Rsa1p [Saccharomyces cerevisiae YJM1574]" 100.00 381 100.00 100.00 6.28e-04 REF NP_015131 "Rsa1p [Saccharomyces cerevisiae S288c]" 100.00 381 100.00 100.00 6.28e-04 SP Q08932 "RecName: Full=Ribosome assembly 1 protein" 100.00 381 100.00 100.00 6.28e-04 TPG DAA11241 "TPA: Rsa1p [Saccharomyces cerevisiae S288c]" 100.00 381 100.00 100.00 6.28e-04 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Rsa1p238-259 'Baker's yeast' 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Rsa1p238-259 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Rsa1p238-259 2 mM 'natural abundance' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 150 mM 'natural abundance' H2O 95 % 'natural abundance' D2O 5 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CNS _Saveframe_category software _Name CNS _Version . loop_ _Vendor _Address _Electronic_address 'Brunger, Adams, Clore, Gros, Nilges and Read' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_CYANA _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_PREDITOR _Saveframe_category software _Name PREDITOR _Version . loop_ _Vendor _Address _Electronic_address 'Wishart DS.' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_TOCSY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ save_2D_1H-1H_TOCSY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_2D_1H-1H_COSY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H COSY' _Sample_label $sample_1 save_ save_2D_1H-1H_COSY_10 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H COSY' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC-TOCSY_11 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC-TOCSY' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC-TOCSY_12 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC-TOCSY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 150 . mM pH 3.7 . pH pressure 1 . atm temperature 293 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 150 . mM pH 3.7 . pH pressure 1 . atm temperature 305 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0 external indirect cylindrical 'separate tube (no insert) not similar to the experimental sample tube' . 0.251449530 DSS H 1 'methyl protons' ppm 0 external direct cylindrical 'separate tube (no insert) not similar to the experimental sample tube' . 1.0 DSS N 15 'methyl protons' ppm 0 external indirect cylindrical 'separate tube (no insert) not similar to the experimental sample tube' . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '2D 1H-1H NOESY' '2D 1H-1H TOCSY' '2D 1H-13C HSQC' '2D 1H-1H COSY' '2D 1H-13C HSQC-TOCSY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Rsa1p238-259 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 THR HA H 3.813 0.020 . 2 1 1 THR HB H 4.106 0.020 . 3 1 1 THR HG2 H 1.175 0.020 . 4 1 1 THR CA C 58.460 0.300 . 5 1 1 THR CB C 66.426 0.300 . 6 1 1 THR CG2 C 18.620 0.300 . 7 2 2 ASP H H 8.880 0.020 . 8 2 2 ASP HA H 4.534 0.020 . 9 2 2 ASP HB2 H 2.691 0.020 . 10 2 2 ASP HB3 H 2.617 0.020 . 11 2 2 ASP CA C 51.548 0.300 . 12 2 2 ASP CB C 36.798 0.300 . 13 2 2 ASP N N 123.509 0.300 . 14 3 3 GLU H H 8.445 0.020 . 15 3 3 GLU HA H 4.102 0.020 . 16 3 3 GLU HB2 H 1.912 0.020 . 17 3 3 GLU HB3 H 1.836 0.020 . 18 3 3 GLU HG2 H 2.270 0.020 . 19 3 3 GLU HG3 H 2.270 0.020 . 20 3 3 GLU CA C 54.167 0.300 . 21 3 3 GLU CB C 26.028 0.300 . 22 3 3 GLU CG C 30.833 0.300 . 23 3 3 GLU N N 121.439 0.300 . 24 4 4 ASP H H 8.187 0.020 . 25 4 4 ASP HA H 4.413 0.020 . 26 4 4 ASP HB2 H 2.605 0.020 . 27 4 4 ASP HB3 H 2.605 0.020 . 28 4 4 ASP CA C 51.773 0.300 . 29 4 4 ASP CB C 36.728 0.300 . 30 4 4 ASP N N 120.428 0.300 . 31 5 5 VAL H H 7.801 0.020 . 32 5 5 VAL HA H 3.769 0.020 . 33 5 5 VAL HB H 1.972 0.020 . 34 5 5 VAL HG1 H 0.799 0.020 . 35 5 5 VAL HG2 H 0.840 0.020 . 36 5 5 VAL CA C 61.399 0.300 . 37 5 5 VAL CB C 29.347 0.300 . 38 5 5 VAL CG1 C 18.229 0.300 . 39 5 5 VAL CG2 C 18.344 0.300 . 40 5 5 VAL N N 121.028 0.300 . 41 6 6 LYS H H 8.029 0.020 . 42 6 6 LYS HA H 3.962 0.020 . 43 6 6 LYS HB2 H 1.651 0.020 . 44 6 6 LYS HB3 H 1.651 0.020 . 45 6 6 LYS HG2 H 1.333 0.020 . 46 6 6 LYS HG3 H 1.222 0.020 . 47 6 6 LYS HD2 H 1.517 0.020 . 48 6 6 LYS HD3 H 1.517 0.020 . 49 6 6 LYS HE2 H 2.598 0.020 . 50 6 6 LYS HE3 H 2.598 0.020 . 51 6 6 LYS CA C 55.492 0.300 . 52 6 6 LYS CB C 29.679 0.300 . 53 6 6 LYS CG C 22.308 0.300 . 54 6 6 LYS CD C 26.405 0.300 . 55 6 6 LYS CE C 37.144 0.300 . 56 6 6 LYS N N 122.306 0.300 . 57 7 7 LYS H H 7.885 0.020 . 58 7 7 LYS HA H 4.010 0.020 . 59 7 7 LYS HB2 H 1.648 0.020 . 60 7 7 LYS HB3 H 1.648 0.020 . 61 7 7 LYS HG2 H 1.271 0.020 . 62 7 7 LYS HG3 H 1.166 0.020 . 63 7 7 LYS HD2 H 1.498 0.020 . 64 7 7 LYS HD3 H 1.498 0.020 . 65 7 7 LYS HE2 H 2.799 0.020 . 66 7 7 LYS HE3 H 2.799 0.020 . 67 7 7 LYS CA C 55.117 0.300 . 68 7 7 LYS CB C 29.650 0.300 . 69 7 7 LYS CG C 22.108 0.300 . 70 7 7 LYS CD C 26.359 0.300 . 71 7 7 LYS CE C 39.301 0.300 . 72 7 7 LYS N N 120.339 0.300 . 73 8 8 TRP H H 7.928 0.020 . 74 8 8 TRP HA H 4.388 0.020 . 75 8 8 TRP HB2 H 3.226 0.020 . 76 8 8 TRP HB3 H 3.179 0.020 . 77 8 8 TRP HD1 H 7.122 0.020 . 78 8 8 TRP HE1 H 10.018 0.020 . 79 8 8 TRP HE3 H 7.420 0.020 . 80 8 8 TRP HZ2 H 7.328 0.020 . 81 8 8 TRP HZ3 H 6.964 0.020 . 82 8 8 TRP HH2 H 7.057 0.020 . 83 8 8 TRP CA C 56.174 0.300 . 84 8 8 TRP CB C 26.354 0.300 . 85 8 8 TRP CD1 C 124.394 0.300 . 86 8 8 TRP CE3 C 117.996 0.300 . 87 8 8 TRP CZ2 C 111.761 0.300 . 88 8 8 TRP CZ3 C 119.100 0.300 . 89 8 8 TRP CH2 C 121.783 0.300 . 90 8 8 TRP N N 120.854 0.300 . 91 8 8 TRP NE1 N 129.296 0.300 . 92 9 9 ARG H H 8.088 0.020 . 93 9 9 ARG HA H 3.807 0.020 . 94 9 9 ARG HB2 H 1.667 0.020 . 95 9 9 ARG HB3 H 1.667 0.020 . 96 9 9 ARG HG2 H 1.519 0.020 . 97 9 9 ARG HG3 H 1.379 0.020 . 98 9 9 ARG HD2 H 3.004 0.020 . 99 9 9 ARG HD3 H 3.004 0.020 . 100 9 9 ARG HE H 7.093 0.020 . 101 9 9 ARG CA C 55.395 0.300 . 102 9 9 ARG CB C 27.633 0.300 . 103 9 9 ARG CG C 24.726 0.300 . 104 9 9 ARG CD C 40.765 0.300 . 105 9 9 ARG N N 120.280 0.300 . 106 9 9 ARG NE N 120.778 0.300 . 107 10 10 GLU H H 7.958 0.020 . 108 10 10 GLU HA H 4.000 0.020 . 109 10 10 GLU HB2 H 1.958 0.020 . 110 10 10 GLU HB3 H 1.958 0.020 . 111 10 10 GLU HG2 H 2.334 0.020 . 112 10 10 GLU HG3 H 2.306 0.020 . 113 10 10 GLU CA C 54.782 0.300 . 114 10 10 GLU CB C 25.907 0.300 . 115 10 10 GLU CG C 31.222 0.300 . 116 10 10 GLU N N 119.696 0.300 . 117 11 11 GLU H H 8.094 0.020 . 118 11 11 GLU HA H 4.038 0.020 . 119 11 11 GLU HB2 H 1.943 0.020 . 120 11 11 GLU HB3 H 1.943 0.020 . 121 11 11 GLU HG2 H 2.342 0.020 . 122 11 11 GLU HG3 H 2.305 0.020 . 123 11 11 GLU CA C 54.432 0.300 . 124 11 11 GLU CB C 25.862 0.300 . 125 11 11 GLU CG C 30.960 0.300 . 126 11 11 GLU N N 120.334 0.300 . 127 12 12 ARG H H 7.955 0.020 . 128 12 12 ARG HA H 3.948 0.020 . 129 12 12 ARG HB2 H 1.590 0.020 . 130 12 12 ARG HB3 H 1.507 0.020 . 131 12 12 ARG HG2 H 1.315 0.020 . 132 12 12 ARG HG3 H 1.315 0.020 . 133 12 12 ARG HD2 H 2.803 0.020 . 134 12 12 ARG HD3 H 2.803 0.020 . 135 12 12 ARG HE H 6.940 0.020 . 136 12 12 ARG CA C 54.512 0.300 . 137 12 12 ARG CB C 27.227 0.300 . 138 12 12 ARG CG C 24.082 0.300 . 139 12 12 ARG CD C 40.369 0.300 . 140 12 12 ARG N N 120.480 0.300 . 141 12 12 ARG NE N 120.592 0.300 . 142 13 13 LYS H H 7.818 0.020 . 143 13 13 LYS HA H 3.989 0.020 . 144 13 13 LYS HB2 H 1.699 0.020 . 145 13 13 LYS HB3 H 1.635 0.020 . 146 13 13 LYS HG2 H 1.315 0.020 . 147 13 13 LYS HG3 H 1.244 0.020 . 148 13 13 LYS HD2 H 1.507 0.020 . 149 13 13 LYS HD3 H 1.507 0.020 . 150 13 13 LYS HE2 H 2.796 0.020 . 151 13 13 LYS HE3 H 2.796 0.020 . 152 13 13 LYS CA C 54.843 0.300 . 153 13 13 LYS CB C 29.866 0.300 . 154 13 13 LYS CG C 22.150 0.300 . 155 13 13 LYS CD C 26.373 0.300 . 156 13 13 LYS CE C 39.301 0.300 . 157 13 13 LYS N N 120.644 0.300 . 158 14 14 LYS H H 7.901 0.020 . 159 14 14 LYS HA H 3.945 0.020 . 160 14 14 LYS HB2 H 1.612 0.020 . 161 14 14 LYS HB3 H 1.539 0.020 . 162 14 14 LYS HG2 H 1.283 0.020 . 163 14 14 LYS HG3 H 1.196 0.020 . 164 14 14 LYS HD2 H 1.492 0.020 . 165 14 14 LYS HD3 H 1.492 0.020 . 166 14 14 LYS HE2 H 2.798 0.020 . 167 14 14 LYS HE3 H 2.798 0.020 . 168 14 14 LYS CA C 54.679 0.300 . 169 14 14 LYS CB C 29.688 0.300 . 170 14 14 LYS CG C 21.921 0.300 . 171 14 14 LYS CD C 26.339 0.300 . 172 14 14 LYS CE C 39.305 0.300 . 173 14 14 LYS N N 120.297 0.300 . 174 15 15 MET H H 7.954 0.020 . 175 15 15 MET HA H 4.165 0.020 . 176 15 15 MET HB2 H 1.871 0.020 . 177 15 15 MET HB3 H 1.871 0.020 . 178 15 15 MET HG2 H 2.414 0.020 . 179 15 15 MET HG3 H 2.330 0.020 . 180 15 15 MET HE H 1.894 0.020 . 181 15 15 MET CA C 53.765 0.300 . 182 15 15 MET CB C 29.593 0.300 . 183 15 15 MET CG C 29.162 0.300 . 184 15 15 MET CE C 14.085 0.300 . 185 15 15 MET N N 119.294 0.300 . 186 16 16 TRP H H 7.876 0.020 . 187 16 16 TRP HA H 4.455 0.020 . 188 16 16 TRP HB2 H 3.206 0.020 . 189 16 16 TRP HB3 H 3.150 0.020 . 190 16 16 TRP HD1 H 7.088 0.020 . 191 16 16 TRP HE1 H 10.047 0.020 . 192 16 16 TRP HE3 H 7.476 0.020 . 193 16 16 TRP HZ2 H 7.346 0.020 . 194 16 16 TRP HZ3 H 7.002 0.020 . 195 16 16 TRP HH2 H 7.090 0.020 . 196 16 16 TRP CA C 55.259 0.300 . 197 16 16 TRP CB C 26.358 0.300 . 198 16 16 TRP CD1 C 124.256 0.300 . 199 16 16 TRP CE3 C 117.996 0.300 . 200 16 16 TRP CZ2 C 111.874 0.300 . 201 16 16 TRP CZ3 C 119.277 0.300 . 202 16 16 TRP CH2 C 121.864 0.300 . 203 16 16 TRP N N 121.134 0.300 . 204 16 16 TRP NE1 N 129.352 0.300 . 205 17 17 LEU H H 7.771 0.020 . 206 17 17 LEU HA H 4.027 0.020 . 207 17 17 LEU HB2 H 1.464 0.020 . 208 17 17 LEU HB3 H 1.368 0.020 . 209 17 17 LEU HG H 1.364 0.020 . 210 17 17 LEU HD1 H 0.695 0.020 . 211 17 17 LEU HD2 H 0.737 0.020 . 212 17 17 LEU CA C 52.928 0.300 . 213 17 17 LEU CB C 39.534 0.300 . 214 17 17 LEU CG C 24.026 0.300 . 215 17 17 LEU CD1 C 20.671 0.300 . 216 17 17 LEU CD2 C 22.251 0.300 . 217 17 17 LEU N N 121.626 0.300 . 218 18 18 LEU H H 7.707 0.020 . 219 18 18 LEU HA H 4.103 0.020 . 220 18 18 LEU HB2 H 1.506 0.020 . 221 18 18 LEU HB3 H 1.439 0.020 . 222 18 18 LEU HG H 1.486 0.020 . 223 18 18 LEU HD1 H 0.726 0.020 . 224 18 18 LEU HD2 H 0.780 0.020 . 225 18 18 LEU CA C 52.662 0.300 . 226 18 18 LEU CB C 39.514 0.300 . 227 18 18 LEU CG C 24.160 0.300 . 228 18 18 LEU CD1 C 20.747 0.300 . 229 18 18 LEU CD2 C 22.106 0.300 . 230 18 18 LEU N N 121.161 0.300 . 231 19 19 LYS H H 7.906 0.020 . 232 19 19 LYS HA H 4.124 0.020 . 233 19 19 LYS HB2 H 1.670 0.020 . 234 19 19 LYS HB3 H 1.614 0.020 . 235 19 19 LYS HG2 H 1.269 0.020 . 236 19 19 LYS HG3 H 1.180 0.020 . 237 19 19 LYS HD2 H 1.492 0.020 . 238 19 19 LYS HD3 H 1.492 0.020 . 239 19 19 LYS HE2 H 2.799 0.020 . 240 19 19 LYS HE3 H 2.799 0.020 . 241 19 19 LYS CA C 53.666 0.300 . 242 19 19 LYS CB C 29.930 0.300 . 243 19 19 LYS CG C 21.924 0.300 . 244 19 19 LYS CD C 26.340 0.300 . 245 19 19 LYS CE C 39.326 0.300 . 246 19 19 LYS N N 121.494 0.300 . 247 20 20 ILE H H 7.918 0.020 . 248 20 20 ILE HA H 4.044 0.020 . 249 20 20 ILE HB H 1.703 0.020 . 250 20 20 ILE HG12 H 1.265 0.020 . 251 20 20 ILE HG13 H 1.004 0.020 . 252 20 20 ILE HG2 H 0.709 0.020 . 253 20 20 ILE HD1 H 0.672 0.020 . 254 20 20 ILE CA C 58.349 0.300 . 255 20 20 ILE CB C 35.926 0.300 . 256 20 20 ILE CG1 C 24.289 0.300 . 257 20 20 ILE CG2 C 14.692 0.300 . 258 20 20 ILE CD1 C 10.153 0.300 . 259 20 20 ILE N N 121.282 0.300 . 260 21 21 SER H H 8.125 0.020 . 261 21 21 SER HA H 4.341 0.020 . 262 21 21 SER HB2 H 3.705 0.020 . 263 21 21 SER HB3 H 3.705 0.020 . 264 21 21 SER CA C 55.333 0.300 . 265 21 21 SER CB C 61.220 0.300 . 266 21 21 SER N N 119.658 0.300 . 267 22 22 ASN H H 8.030 0.020 . 268 22 22 ASN HA H 4.419 0.020 . 269 22 22 ASN HB2 H 2.606 0.020 . 270 22 22 ASN HB3 H 2.606 0.020 . 271 22 22 ASN HD21 H 7.415 0.020 . 272 22 22 ASN HD22 H 6.691 0.020 . 273 22 22 ASN CA C 51.346 0.300 . 274 22 22 ASN CB C 37.162 0.300 . 275 22 22 ASN N N 124.885 0.300 . 276 22 22 ASN ND2 N 124.607 0.300 . stop_ save_ save_assigned_chem_shift_list_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '2D 1H-1H NOESY' '2D 1H-1H TOCSY' '2D 1H-13C HSQC' '2D 1H-1H COSY' '2D 1H-13C HSQC-TOCSY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Rsa1p238-259 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 THR HA H 3.944 0.020 . 2 1 1 THR HB H 4.235 0.020 . 3 1 1 THR HG2 H 1.296 0.020 . 4 2 2 ASP H H 8.920 0.020 . 5 2 2 ASP HA H 4.675 0.020 . 6 2 2 ASP HB2 H 2.819 0.020 . 7 2 2 ASP HB3 H 2.745 0.020 . 8 3 3 GLU H H 8.496 0.020 . 9 3 3 GLU HA H 4.244 0.020 . 10 3 3 GLU HB2 H 2.050 0.020 . 11 3 3 GLU HB3 H 1.960 0.020 . 12 3 3 GLU HG2 H 2.389 0.020 . 13 3 3 GLU HG3 H 2.389 0.020 . 14 4 4 ASP H H 8.284 0.020 . 15 4 4 ASP HA H 4.560 0.020 . 16 4 4 ASP HB2 H 2.740 0.020 . 17 4 4 ASP HB3 H 2.740 0.020 . 18 5 5 VAL H H 7.871 0.020 . 19 5 5 VAL HA H 3.913 0.020 . 20 5 5 VAL HB H 2.098 0.020 . 21 5 5 VAL HG1 H 0.921 0.020 . 22 5 5 VAL HG2 H 0.957 0.020 . 23 6 6 LYS H H 8.101 0.020 . 24 6 6 LYS HA H 4.105 0.020 . 25 6 6 LYS HB2 H 1.758 0.020 . 26 6 6 LYS HB3 H 1.758 0.020 . 27 6 6 LYS HG2 H 1.449 0.020 . 28 6 6 LYS HG3 H 1.351 0.020 . 29 6 6 LYS HD2 H 1.649 0.020 . 30 6 6 LYS HD3 H 1.649 0.020 . 31 6 6 LYS HE2 H 2.598 0.020 . 32 6 6 LYS HE3 H 2.598 0.020 . 33 7 7 LYS H H 7.950 0.020 . 34 7 7 LYS HA H 4.144 0.020 . 35 7 7 LYS HB2 H 1.648 0.020 . 36 7 7 LYS HB3 H 1.648 0.020 . 37 7 7 LYS HG2 H 1.371 0.020 . 38 7 7 LYS HG3 H 1.277 0.020 . 39 7 7 LYS HD2 H 1.498 0.020 . 40 7 7 LYS HD3 H 1.498 0.020 . 41 7 7 LYS HE2 H 2.947 0.020 . 42 7 7 LYS HE3 H 2.947 0.020 . 43 8 8 TRP H H 7.978 0.020 . 44 8 8 TRP HA H 4.541 0.020 . 45 8 8 TRP HB2 H 3.337 0.020 . 46 8 8 TRP HB3 H 3.298 0.020 . 47 8 8 TRP HD1 H 7.245 0.020 . 48 8 8 TRP HE1 H 10.107 0.020 . 49 8 8 TRP HE3 H 7.553 0.020 . 50 8 8 TRP HZ2 H 7.463 0.020 . 51 8 8 TRP HZ3 H 7.093 0.020 . 52 8 8 TRP HH2 H 7.189 0.020 . 53 9 9 ARG H H 8.123 0.020 . 54 9 9 ARG HA H 3.981 0.020 . 55 9 9 ARG HB2 H 1.792 0.020 . 56 9 9 ARG HB3 H 1.792 0.020 . 57 9 9 ARG HG2 H 1.620 0.020 . 58 9 9 ARG HG3 H 1.497 0.020 . 59 9 9 ARG HD2 H 3.132 0.020 . 60 9 9 ARG HD3 H 3.132 0.020 . 61 9 9 ARG HE H 7.181 0.020 . 62 10 10 GLU H H 8.037 0.020 . 63 10 10 GLU HA H 4.149 0.020 . 64 10 10 GLU HB2 H 2.075 0.020 . 65 10 10 GLU HB3 H 2.075 0.020 . 66 10 10 GLU HG2 H 2.444 0.020 . 67 10 10 GLU HG3 H 2.421 0.020 . 68 11 11 GLU H H 8.182 0.020 . 69 11 11 GLU HA H 4.193 0.020 . 70 11 11 GLU HB2 H 2.064 0.020 . 71 11 11 GLU HB3 H 2.064 0.020 . 72 11 11 GLU HG2 H 2.456 0.020 . 73 11 11 GLU HG3 H 2.420 0.020 . 74 12 12 ARG H H 8.058 0.020 . 75 12 12 ARG HA H 4.115 0.020 . 76 12 12 ARG HB2 H 1.743 0.020 . 77 12 12 ARG HB3 H 1.657 0.020 . 78 12 12 ARG HG2 H 1.486 0.020 . 79 12 12 ARG HG3 H 1.486 0.020 . 80 12 12 ARG HD2 H 2.978 0.020 . 81 12 12 ARG HD3 H 2.978 0.020 . 82 12 12 ARG HE H 7.063 0.020 . 83 13 13 LYS H H 7.934 0.020 . 84 13 13 LYS HA H 4.130 0.020 . 85 13 13 LYS HB2 H 1.815 0.020 . 86 13 13 LYS HB3 H 1.753 0.020 . 87 13 13 LYS HG2 H 1.443 0.020 . 88 13 13 LYS HG3 H 1.370 0.020 . 89 13 13 LYS HD2 H 1.618 0.020 . 90 13 13 LYS HD3 H 1.618 0.020 . 91 13 13 LYS HE2 H 2.947 0.020 . 92 13 13 LYS HE3 H 2.947 0.020 . 93 14 14 LYS H H 7.999 0.020 . 94 14 14 LYS HA H 4.085 0.020 . 95 14 14 LYS HB2 H 1.809 0.020 . 96 14 14 LYS HB3 H 1.724 0.020 . 97 14 14 LYS HG2 H 1.399 0.020 . 98 14 14 LYS HG3 H 1.399 0.020 . 99 14 14 LYS HD2 H 1.641 0.020 . 100 14 14 LYS HD3 H 1.641 0.020 . 101 14 14 LYS HE2 H 2.946 0.020 . 102 14 14 LYS HE3 H 2.946 0.020 . 103 15 15 MET H H 8.044 0.020 . 104 15 15 MET HA H 4.300 0.020 . 105 15 15 MET HB2 H 1.987 0.020 . 106 15 15 MET HB3 H 1.987 0.020 . 107 15 15 MET HG2 H 2.526 0.020 . 108 15 15 MET HG3 H 2.446 0.020 . 109 15 15 MET HE H 2.021 0.020 . 110 16 16 TRP H H 7.939 0.020 . 111 16 16 TRP HA H 4.602 0.020 . 112 16 16 TRP HB2 H 3.323 0.020 . 113 16 16 TRP HB3 H 3.271 0.020 . 114 16 16 TRP HD1 H 7.218 0.020 . 115 16 16 TRP HE1 H 10.133 0.020 . 116 16 16 TRP HE3 H 7.606 0.020 . 117 16 16 TRP HZ2 H 7.477 0.020 . 118 16 16 TRP HZ3 H 7.130 0.020 . 119 16 16 TRP HH2 H 7.210 0.020 . 120 17 17 LEU H H 7.829 0.020 . 121 17 17 LEU HA H 4.176 0.020 . 122 17 17 LEU HB2 H 1.561 0.020 . 123 17 17 LEU HB3 H 1.505 0.020 . 124 17 17 LEU HG H 1.459 0.020 . 125 17 17 LEU HD1 H 0.814 0.020 . 126 17 17 LEU HD2 H 0.857 0.020 . 127 18 18 LEU H H 7.798 0.020 . 128 18 18 LEU HA H 4.242 0.020 . 129 18 18 LEU HB2 H 1.621 0.020 . 130 18 18 LEU HB3 H 1.579 0.020 . 131 18 18 LEU HG H 1.621 0.020 . 132 18 18 LEU HD1 H 0.852 0.020 . 133 18 18 LEU HD2 H 0.905 0.020 . 134 19 19 LYS H H 7.998 0.020 . 135 19 19 LYS HA H 4.275 0.020 . 136 20 20 ILE H H 7.985 0.020 . 137 20 20 ILE HA H 4.188 0.020 . 138 20 20 ILE HB H 1.836 0.020 . 139 20 20 ILE HG12 H 1.404 0.020 . 140 20 20 ILE HG13 H 1.136 0.020 . 141 20 20 ILE HG2 H 0.850 0.020 . 142 20 20 ILE HD1 H 0.814 0.020 . 143 21 21 SER H H 8.191 0.020 . 144 21 21 SER HA H 4.479 0.020 . 145 21 21 SER HB2 H 3.831 0.020 . 146 21 21 SER HB3 H 3.831 0.020 . 147 22 22 ASN H H 8.094 0.020 . 148 22 22 ASN HA H 4.540 0.020 . 149 22 22 ASN HB2 H 2.708 0.020 . 150 22 22 ASN HB3 H 2.708 0.020 . 151 22 22 ASN HD21 H 7.475 0.020 . 152 22 22 ASN HD22 H 6.764 0.020 . stop_ save_