data_25621 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N chemical shift assignments for the ground state fold of the single mutant D91R of dimeric KaiB from the Thermosynechococcus elongatus BP-1 cyanobacterial species ; _BMRB_accession_number 25621 _BMRB_flat_file_name bmr25621.str _Entry_type original _Submission_date 2015-05-16 _Accession_date 2015-05-16 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Chang Yonggang . . 2 Cohen Susan . . 3 Phong Connie . . 4 Myers William . . 5 Kim Yong-Ick . . 6 Tseng Roger . . 7 Lin Jenny . . 8 Zhang Li . . 9 Boyd Joseph . . 10 Lee Yvonne . . 11 Kang Shannon . . 12 Lee David . . 13 Li Sheng . . 14 Britt R. . . 15 Rust Michael . . 16 Golden Susan . . 17 LiWang Andy . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 51 "13C chemical shifts" 168 "15N chemical shifts" 51 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2016-08-24 update BMRB 'update entry citation' 2015-07-14 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 25616 'KaiB dimer' 25617 'dimeric KaiB bound to CI' 25618 N-SasA 25619 'N-SasA bound to CI' 25620 'G89A single mutant of dimeric KaiB' 25622 'D91R single mutant of dimeric KaiB (thioredoxin-like fold)' 25623 'G89A,D91R double mutant of dimeric KaiB' 25624 'G89A,D91R double mutant of KaiB' 25625 'G89A,D91R double mutant of KaiB bound to CI' 25626 'G88A,D90R double mutant of KaiB' stop_ _Original_release_date 2015-07-14 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; A Protein Fold Switch Joins the Circadian Oscillator to Clock Output in Cyanobacteria ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 26113641 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Chang Yonggang . . 2 Cohen Susan . . 3 Phong Connie . . 4 Myers William . . 5 Kim Yong-Ick . . 6 Tseng Roger . . 7 Lin Jenny . . 8 Zhang Li . . 9 Boyd Joseph . . 10 Lee Yvonne . . 11 Kang Shannon . . 12 Lee David . . 13 Li Sheng . . 14 Britt R. . . 15 Rust Michael . . 16 Golden Susan . . 17 LiWang Andy . . stop_ _Journal_abbreviation Science _Journal_volume 349 _Journal_issue 6245 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 324 _Page_last 328 _Year 2015 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'D91R single mutant of dimeric KaiB' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'KaiB D91R mutant, chain 1' $FTBN94YYD91RF_gs 'KaiB D91R mutant, chain 2' $FTBN94YYD91RF_gs stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_FTBN94YYD91RF_gs _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common FTBN94YYD91RF_gs _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 110 _Mol_residue_sequence ; DYKDDDDKMAPLRKTAVLKL YVAGNTPNSVRALKTLNNIL EKEFKGVYALKVIDVLKNPQ LAEEDKILATPTLAKVLPPP VRRIIGDLSNREKVLIGLRL LADYKDDDDK ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 ASP 2 2 TYR 3 3 LYS 4 4 ASP 5 5 ASP 6 6 ASP 7 7 ASP 8 8 LYS 9 9 MET 10 10 ALA 11 11 PRO 12 12 LEU 13 13 ARG 14 14 LYS 15 15 THR 16 16 ALA 17 17 VAL 18 18 LEU 19 19 LYS 20 20 LEU 21 21 TYR 22 22 VAL 23 23 ALA 24 24 GLY 25 25 ASN 26 26 THR 27 27 PRO 28 28 ASN 29 29 SER 30 30 VAL 31 31 ARG 32 32 ALA 33 33 LEU 34 34 LYS 35 35 THR 36 36 LEU 37 37 ASN 38 38 ASN 39 39 ILE 40 40 LEU 41 41 GLU 42 42 LYS 43 43 GLU 44 44 PHE 45 45 LYS 46 46 GLY 47 47 VAL 48 48 TYR 49 49 ALA 50 50 LEU 51 51 LYS 52 52 VAL 53 53 ILE 54 54 ASP 55 55 VAL 56 56 LEU 57 57 LYS 58 58 ASN 59 59 PRO 60 60 GLN 61 61 LEU 62 62 ALA 63 63 GLU 64 64 GLU 65 65 ASP 66 66 LYS 67 67 ILE 68 68 LEU 69 69 ALA 70 70 THR 71 71 PRO 72 72 THR 73 73 LEU 74 74 ALA 75 75 LYS 76 76 VAL 77 77 LEU 78 78 PRO 79 79 PRO 80 80 PRO 81 81 VAL 82 82 ARG 83 83 ARG 84 84 ILE 85 85 ILE 86 86 GLY 87 87 ASP 88 88 LEU 89 89 SER 90 90 ASN 91 91 ARG 92 92 GLU 93 93 LYS 94 94 VAL 95 95 LEU 96 96 ILE 97 97 GLY 98 98 LEU 99 99 ARG 100 100 LEU 101 101 LEU 102 102 ALA 103 103 ASP 104 104 TYR 105 105 LYS 106 106 ASP 107 107 ASP 108 108 ASP 109 109 ASP 110 110 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $FTBN94YYD91RF_gs cyanobacteria 146786 Bacteria . Thermosynechococcus elongatus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $FTBN94YYD91RF_gs 'recombinant technology' . Escherichia coli . pET-28b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details ; Sample: [N15,C13]-FLAG-TeKaiB-1-94-Y8A-Y94A-D91R-FLAG (1819 uM); Buffer; 20 mM Tris, 100 mM NaCl, pH 7.0, 10 uM DSS, 0.02% NaN3, 95%H2O/5%D2O; Volume: 320 uL; Tube: shaped ; loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $FTBN94YYD91RF_gs 1819 uM '[U-99% 13C; U-98% 15N]' Tris 20 mM 'natural abundance' NaCl 100 mM 'natural abundance' DSS 10 uM 'natural abundance' NaNa3 0.02 % 'natural abundance' H2O 95 % 'natural abundance' D2O 5 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_Mars _Saveframe_category software _Name Mars _Version . loop_ _Vendor _Address _Electronic_address 'Young-Sang Jung and Markus Zweckstetter' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details 'robust automatic backbone assignment of proteins' save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details 'Bruker 600 MHz AVANCE III spectrometer equipped with a TCI cryoprobe and z-axis pulsed-field gradient capability' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CO)CACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CACB' _Sample_label $sample_1 save_ save_3D_HN(CA)CO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)CO' _Sample_label $sample_1 save_ save_3D_HNCO_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 7.0 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl carbons' MHz 601.1299449 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCACB' '3D HN(CO)CACB' '3D HN(CA)CO' '3D HNCO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'KaiB D91R mutant, chain 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 15 15 THR C C 173.200 . 1 2 15 15 THR CA C 62.790 . 1 3 15 15 THR CB C 70.250 . 1 4 16 16 ALA H H 8.074 . 1 5 16 16 ALA C C 176.300 . 1 6 16 16 ALA CA C 51.420 . 1 7 16 16 ALA CB C 20.790 . 1 8 16 16 ALA N N 127.900 . 1 9 17 17 VAL H H 8.657 . 1 10 17 17 VAL C C 174.100 . 1 11 17 17 VAL CA C 61.600 . 1 12 17 17 VAL CB C 34.090 . 1 13 17 17 VAL N N 121.400 . 1 14 18 18 LEU H H 9.031 . 1 15 18 18 LEU C C 173.200 . 1 16 18 18 LEU CA C 53.360 . 1 17 18 18 LEU CB C 43.130 . 1 18 18 18 LEU N N 130.300 . 1 19 19 19 LYS H H 9.091 . 1 20 19 19 LYS C C 174.500 . 1 21 19 19 LYS CA C 54.920 . 1 22 19 19 LYS CB C 34.230 . 1 23 19 19 LYS N N 126.500 . 1 24 20 20 LEU H H 8.632 . 1 25 20 20 LEU C C 174.200 . 1 26 20 20 LEU N N 123.700 . 1 27 21 21 TYR C C 175.200 . 1 28 21 21 TYR CA C 56.750 . 1 29 21 21 TYR CB C 38.250 . 1 30 22 22 VAL H H 9.308 . 1 31 22 22 VAL C C 174.400 . 1 32 22 22 VAL CA C 58.360 . 1 33 22 22 VAL CB C 34.850 . 1 34 22 22 VAL N N 118.500 . 1 35 23 23 ALA H H 9.256 . 1 36 23 23 ALA C C 175.800 . 1 37 23 23 ALA CA C 50.230 . 1 38 23 23 ALA CB C 18.180 . 1 39 23 23 ALA N N 127.800 . 1 40 24 24 GLY H H 7.996 . 1 41 24 24 GLY C C 171.400 . 1 42 24 24 GLY CA C 44.860 . 1 43 24 24 GLY N N 110.400 . 1 44 25 25 ASN H H 8.012 . 1 45 25 25 ASN C C 174.000 . 1 46 25 25 ASN CA C 52.540 . 1 47 25 25 ASN CB C 42.730 . 1 48 25 25 ASN N N 112.400 . 1 49 26 26 THR H H 9.102 . 1 50 26 26 THR C C 173.300 . 1 51 26 26 THR CA C 58.700 . 1 52 26 26 THR CB C 69.800 . 1 53 26 26 THR N N 113.400 . 1 54 27 27 PRO C C 179.900 . 1 55 27 27 PRO CA C 66.240 . 1 56 27 27 PRO CB C 31.560 . 1 57 28 28 ASN H H 8.522 . 1 58 28 28 ASN C C 178.800 . 1 59 28 28 ASN CA C 55.120 . 1 60 28 28 ASN CB C 37.740 . 1 61 28 28 ASN N N 113.000 . 1 62 29 29 SER H H 8.970 . 1 63 29 29 SER C C 175.900 . 1 64 29 29 SER N N 123.300 . 1 65 30 30 VAL H H 8.375 . 1 66 30 30 VAL C C 179.400 . 1 67 30 30 VAL CA C 67.050 . 1 68 30 30 VAL CB C 31.760 . 1 69 30 30 VAL N N 124.600 . 1 70 31 31 ARG H H 7.339 . 1 71 31 31 ARG C C 178.900 . 1 72 31 31 ARG CA C 59.720 . 1 73 31 31 ARG CB C 30.390 . 1 74 31 31 ARG N N 119.300 . 1 75 32 32 ALA C C 179.800 . 1 76 32 32 ALA CA C 55.210 . 1 77 32 32 ALA CB C 18.550 . 1 78 33 33 LEU H H 8.499 . 1 79 33 33 LEU C C 179.800 . 1 80 33 33 LEU CA C 58.200 . 1 81 33 33 LEU CB C 41.600 . 1 82 33 33 LEU N N 118.300 . 1 83 34 34 LYS H H 7.848 . 1 84 34 34 LYS C C 179.300 . 1 85 34 34 LYS CA C 59.920 . 1 86 34 34 LYS CB C 31.930 . 1 87 34 34 LYS N N 122.100 . 1 88 35 35 THR H H 8.293 . 1 89 35 35 THR C C 176.300 . 1 90 35 35 THR CA C 67.120 . 1 91 35 35 THR CB C 68.550 . 1 92 35 35 THR N N 118.600 . 1 93 36 36 LEU H H 8.836 . 1 94 36 36 LEU C C 177.600 . 1 95 36 36 LEU CA C 58.260 . 1 96 36 36 LEU CB C 41.380 . 1 97 36 36 LEU N N 122.000 . 1 98 37 37 ASN H H 8.217 . 1 99 37 37 ASN C C 177.000 . 1 100 37 37 ASN CA C 57.890 . 1 101 37 37 ASN CB C 39.500 . 1 102 37 37 ASN N N 116.900 . 1 103 38 38 ASN H H 8.075 . 1 104 38 38 ASN C C 177.800 . 1 105 38 38 ASN CA C 56.490 . 1 106 38 38 ASN CB C 38.470 . 1 107 38 38 ASN N N 117.000 . 1 108 39 39 ILE H H 8.520 . 1 109 39 39 ILE C C 178.700 . 1 110 39 39 ILE CA C 65.580 . 1 111 39 39 ILE CB C 38.580 . 1 112 39 39 ILE N N 120.800 . 1 113 40 40 LEU H H 8.428 . 1 114 40 40 LEU C C 179.500 . 1 115 40 40 LEU CA C 58.740 . 1 116 40 40 LEU CB C 40.480 . 1 117 40 40 LEU N N 119.700 . 1 118 41 41 GLU H H 8.198 . 1 119 41 41 GLU C C 177.700 . 1 120 41 41 GLU CA C 59.060 . 1 121 41 41 GLU CB C 30.250 . 1 122 41 41 GLU N N 117.100 . 1 123 42 42 LYS H H 8.086 . 1 124 42 42 LYS C C 178.600 . 1 125 42 42 LYS CA C 58.300 . 1 126 42 42 LYS CB C 33.520 . 1 127 42 42 LYS N N 116.100 . 1 128 43 43 GLU H H 8.540 . 1 129 43 43 GLU C C 177.000 . 1 130 43 43 GLU CA C 57.720 . 1 131 43 43 GLU CB C 31.010 . 1 132 43 43 GLU N N 117.100 . 1 133 44 44 PHE H H 7.660 . 1 134 44 44 PHE C C 175.700 . 1 135 44 44 PHE CA C 56.960 . 1 136 44 44 PHE CB C 39.980 . 1 137 44 44 PHE N N 115.900 . 1 138 45 45 LYS H H 7.721 . 1 139 45 45 LYS C C 178.300 . 1 140 45 45 LYS CA C 58.630 . 1 141 45 45 LYS CB C 31.180 . 1 142 45 45 LYS N N 121.400 . 1 143 46 46 GLY H H 9.179 . 1 144 46 46 GLY C C 174.400 . 1 145 46 46 GLY CA C 45.810 . 1 146 46 46 GLY N N 113.200 . 1 147 47 47 VAL C C 175.900 . 1 148 47 47 VAL CA C 65.080 . 1 149 47 47 VAL CB C 33.490 . 1 150 48 48 TYR H H 7.894 . 1 151 48 48 TYR C C 174.900 . 1 152 48 48 TYR CA C 56.190 . 1 153 48 48 TYR CB C 43.140 . 1 154 48 48 TYR N N 115.000 . 1 155 49 49 ALA H H 8.614 . 1 156 49 49 ALA C C 175.200 . 1 157 49 49 ALA CA C 50.790 . 1 158 49 49 ALA CB C 21.330 . 1 159 49 49 ALA N N 124.400 . 1 160 50 50 LEU H H 8.811 . 1 161 50 50 LEU C C 176.400 . 1 162 50 50 LEU CA C 54.370 . 1 163 50 50 LEU CB C 44.680 . 1 164 50 50 LEU N N 123.500 . 1 165 51 51 LYS H H 8.945 . 1 166 51 51 LYS C C 173.600 . 1 167 51 51 LYS CA C 54.910 . 1 168 51 51 LYS CB C 35.600 . 1 169 51 51 LYS N N 127.800 . 1 170 52 52 VAL H H 8.653 . 1 171 52 52 VAL C C 175.300 . 1 172 52 52 VAL CA C 62.080 . 1 173 52 52 VAL CB C 32.990 . 1 174 52 52 VAL N N 125.100 . 1 175 59 59 PRO C C 178.400 . 1 176 59 59 PRO CA C 64.880 . 1 177 59 59 PRO CB C 31.680 . 1 178 60 60 GLN H H 8.718 . 1 179 60 60 GLN C C 176.700 . 1 180 60 60 GLN CA C 57.020 . 1 181 60 60 GLN CB C 27.670 . 1 182 60 60 GLN N N 119.000 . 1 183 61 61 LEU H H 7.870 . 1 184 61 61 LEU C C 177.200 . 1 185 61 61 LEU CA C 55.220 . 1 186 61 61 LEU CB C 42.110 . 1 187 61 61 LEU N N 121.300 . 1 188 83 83 ARG C C 178.400 . 1 189 83 83 ARG CA C 59.550 . 1 190 83 83 ARG CB C 30.000 . 1 191 84 84 ILE H H 6.973 . 1 192 84 84 ILE C C 178.200 . 1 193 84 84 ILE CA C 64.840 . 1 194 84 84 ILE CB C 38.180 . 1 195 84 84 ILE N N 119.300 . 1 196 85 85 ILE H H 8.014 . 1 197 85 85 ILE C C 178.300 . 1 198 85 85 ILE CA C 65.040 . 1 199 85 85 ILE CB C 36.660 . 1 200 85 85 ILE N N 119.600 . 1 201 86 86 GLY H H 8.326 . 1 202 86 86 GLY C C 176.000 . 1 203 86 86 GLY CA C 47.340 . 1 204 86 86 GLY N N 108.100 . 1 205 87 87 ASP H H 7.987 . 1 206 87 87 ASP C C 180.000 . 1 207 87 87 ASP CA C 57.970 . 1 208 87 87 ASP CB C 40.220 . 1 209 87 87 ASP N N 123.700 . 1 210 88 88 LEU H H 8.554 . 1 211 88 88 LEU C C 177.900 . 1 212 88 88 LEU CA C 58.050 . 1 213 88 88 LEU CB C 43.280 . 1 214 88 88 LEU N N 120.000 . 1 215 89 89 SER H H 8.232 . 1 216 89 89 SER C C 175.600 . 1 217 89 89 SER CA C 60.430 . 1 218 89 89 SER CB C 63.970 . 1 219 89 89 SER N N 113.000 . 1 220 90 90 ASN H H 8.781 . 1 221 90 90 ASN C C 175.800 . 1 222 90 90 ASN CA C 55.550 . 1 223 90 90 ASN CB C 39.810 . 1 224 90 90 ASN N N 116.600 . 1 225 91 91 ARG H H 7.975 . 1 226 91 91 ARG C C 177.200 . 1 227 91 91 ARG CA C 58.240 . 1 228 91 91 ARG CB C 30.830 . 1 229 91 91 ARG N N 118.400 . 1 230 92 92 GLU H H 8.259 . 1 231 92 92 GLU C C 175.900 . 1 232 92 92 GLU CA C 56.420 . 1 233 92 92 GLU CB C 30.000 . 1 234 92 92 GLU N N 114.400 . 1 235 93 93 LYS H H 6.821 . 1 236 93 93 LYS CA C 57.670 . 1 237 93 93 LYS CB C 29.400 . 1 238 93 93 LYS N N 109.900 . 1 239 96 96 ILE C C 176.300 . 1 240 96 96 ILE CA C 61.160 . 1 241 96 96 ILE CB C 38.930 . 1 242 97 97 GLY H H 7.729 . 1 243 97 97 GLY C C 170.200 . 1 244 97 97 GLY CA C 45.760 . 1 245 97 97 GLY N N 105.800 . 1 246 98 98 LEU H H 8.729 . 1 247 98 98 LEU C C 174.900 . 1 248 98 98 LEU CA C 54.710 . 1 249 98 98 LEU CB C 46.720 . 1 250 98 98 LEU N N 119.400 . 1 251 99 99 ARG H H 9.183 . 1 252 99 99 ARG C C 173.200 . 1 253 99 99 ARG CA C 55.840 . 1 254 99 99 ARG CB C 35.280 . 1 255 99 99 ARG N N 119.900 . 1 256 100 100 LEU H H 8.648 . 1 257 100 100 LEU C C 175.600 . 1 258 100 100 LEU CA C 53.820 . 1 259 100 100 LEU CB C 44.380 . 1 260 100 100 LEU N N 125.800 . 1 261 101 101 LEU H H 9.371 . 1 262 101 101 LEU C C 175.600 . 1 263 101 101 LEU CA C 54.060 . 1 264 101 101 LEU CB C 42.850 . 1 265 101 101 LEU N N 128.700 . 1 266 102 102 ALA H H 8.566 . 1 267 102 102 ALA C C 176.600 . 1 268 102 102 ALA CA C 52.430 . 1 269 102 102 ALA CB C 19.800 . 1 270 102 102 ALA N N 126.200 . 1 stop_ save_