data_1135 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Antimicrobial Peptide, Tachyplesin I, Isolated from Hemocytes of the Horseshoe Crab (Tachypleus tridentatus) ; _BMRB_accession_number 1135 _BMRB_flat_file_name bmr1135.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kawano Keiichi . . 2 Yoneya Takashi . . 3 Miyata Toshiyuki . . 4 Yoshikawa Katsuhiro . . 5 Tokunaga Fuminori . . 6 Terada Yoshihiro . . 7 Iwanaga Sadaaki . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 111 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-14 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Kawano, Keiichi, Yoneya, Takashi, Miyata, Toshiyuki, Yoshikawa, Katsuhiro, Tokunaga, Fuminori, Terada, Yoshihiro, Iwanaga, Sadaaki, "Antimicrobial Peptide, Tachyplesin I, Isolated from Hemocytes of the Horseshoe Crab (Tachypleus tridentatus)," J. Biol. Chem. 265 (26), 15365-15367 (1990). ; _Citation_title ; Antimicrobial Peptide, Tachyplesin I, Isolated from Hemocytes of the Horseshoe Crab (Tachypleus tridentatus) ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kawano Keiichi . . 2 Yoneya Takashi . . 3 Miyata Toshiyuki . . 4 Yoshikawa Katsuhiro . . 5 Tokunaga Fuminori . . 6 Terada Yoshihiro . . 7 Iwanaga Sadaaki . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 265 _Journal_issue 26 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 15365 _Page_last 15367 _Year 1990 _Details . save_ ################################## # Molecular system description # ################################## save_system_tachyplesin_I _Saveframe_category molecular_system _Mol_system_name 'tachyplesin I' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'tachyplesin I' $tachyplesin_I stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_tachyplesin_I _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'tachyplesin I' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 16 _Mol_residue_sequence KWCFRVCYRGICYRRC loop_ _Residue_seq_code _Residue_label 1 LYS 2 TRP 3 CYS 4 PHE 5 ARG 6 VAL 7 CYS 8 TYR 9 ARG 10 GLY 11 ILE 12 CYS 13 TYR 14 ARG 15 ARG 16 CYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-06 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 11538 Tachyplesin_I 100.00 18 100.00 100.00 2.72e-01 BMRB 11539 Tachyplesin_I 100.00 18 100.00 100.00 2.72e-01 PDB 1MA2 "Tachyplesin I Wild Type Peptide Nmr Structure" 100.00 17 100.00 100.00 2.77e-01 PDB 1MA5 "Tachyplesin I Solution Structure In The Presence Of 300mm Dodecylphosphocholine Micelles" 100.00 17 100.00 100.00 2.77e-01 PDB 1WO0 "Solution Structure Of Tachyplesin I In H2o" 100.00 18 100.00 100.00 2.72e-01 PDB 1WO1 "Tachyplesin I In Dodecylphosphocholine Micelles" 100.00 18 100.00 100.00 2.72e-01 PDB 2MDB "Tachyplesin I In The Presence Of Lipopolysaccharide" 100.00 18 100.00 100.00 2.72e-01 PDB 2RTV "Tachyplesin I In Water" 100.00 18 100.00 100.00 2.72e-01 GB AAA63538 "tachyplesin [Tachypleus tridentatus]" 100.00 77 100.00 100.00 1.86e-02 SP P14213 "RecName: Full=Tachyplesin-1; AltName: Full=Tachyplesin I; Flags: Precursor [Tachypleus tridentatus]" 100.00 77 100.00 100.00 1.86e-02 SP P69135 "RecName: Full=Tachyplesin-1; AltName: Full=Tachyplesin I [Tachypleus gigas]" 100.00 17 100.00 100.00 2.77e-01 SP P69136 "RecName: Full=Tachyplesin-1; AltName: Full=Tachyplesin I [Carcinoscorpius rotundicauda]" 100.00 17 100.00 100.00 2.77e-01 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain _Tissue $tachyplesin_I 'horseshoe crab' 6853 Eukaryota Metazoa Tachyplaus tridentatus generic hemocytes stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $tachyplesin_I 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 3.8 . na temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'tachyplesin I' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 LYS HA H 4.17 . 1 2 . 1 LYS HB2 H 1.86 . 1 3 . 1 LYS HB3 H 1.86 . 1 4 . 1 LYS HG2 H 1.42 . 1 5 . 1 LYS HG3 H 1.42 . 1 6 . 1 LYS HD2 H 1.63 . 1 7 . 1 LYS HD3 H 1.63 . 1 8 . 1 LYS HE2 H 2.95 . 1 9 . 1 LYS HE3 H 2.95 . 1 10 . 2 TRP H H 8.88 . 1 11 . 2 TRP HA H 4.96 . 1 12 . 2 TRP HB2 H 3.33 . 1 13 . 2 TRP HB3 H 3.33 . 1 14 . 2 TRP HD1 H 7.26 . 1 15 . 2 TRP HE1 H 10.08 . 1 16 . 2 TRP HE3 H 7.66 . 1 17 . 2 TRP HZ2 H 7.43 . 1 18 . 2 TRP HZ3 H 7.08 . 1 19 . 2 TRP HH2 H 7.13 . 1 20 . 3 CYS H H 8.28 . 1 21 . 3 CYS HA H 5.46 . 1 22 . 3 CYS HB2 H 2.57 . 2 23 . 3 CYS HB3 H 2.98 . 2 24 . 4 PHE H H 8.72 . 1 25 . 4 PHE HA H 4.79 . 1 26 . 4 PHE HB2 H 2.98 . 1 27 . 4 PHE HB3 H 2.98 . 1 28 . 4 PHE HD1 H 7.16 . 1 29 . 4 PHE HD2 H 7.16 . 1 30 . 4 PHE HE1 H 7.06 . 1 31 . 4 PHE HE2 H 7.06 . 1 32 . 4 PHE HZ H 6.86 . 1 33 . 5 ARG H H 8.51 . 1 34 . 5 ARG HA H 4.95 . 1 35 . 5 ARG HB2 H 1.68 . 1 36 . 5 ARG HB3 H 1.68 . 1 37 . 5 ARG HG2 H 1.46 . 1 38 . 5 ARG HG3 H 1.46 . 1 39 . 5 ARG HD2 H 3.1 . 1 40 . 5 ARG HD3 H 3.1 . 1 41 . 5 ARG HE H 7.16 . 1 42 . 6 VAL H H 8.84 . 1 43 . 6 VAL HA H 4.37 . 1 44 . 6 VAL HB H .9 . 1 45 . 6 VAL HG1 H 1.71 . 1 46 . 6 VAL HG2 H 1.71 . 1 47 . 7 CYS H H 8.58 . 1 48 . 7 CYS HA H 5.62 . 1 49 . 7 CYS HB2 H 2.66 . 2 50 . 7 CYS HB3 H 3.04 . 2 51 . 8 TYR H H 9.09 . 1 52 . 8 TYR HA H 4.66 . 1 53 . 8 TYR HB2 H 3.03 . 1 54 . 8 TYR HB3 H 3.03 . 1 55 . 8 TYR HD1 H 7.21 . 1 56 . 8 TYR HD2 H 7.21 . 1 57 . 8 TYR HE1 H 6.84 . 1 58 . 8 TYR HE2 H 6.84 . 1 59 . 9 ARG H H 9.19 . 1 60 . 9 ARG HA H 3.71 . 1 61 . 9 ARG HB2 H 1.62 . 2 62 . 9 ARG HB3 H 1.91 . 2 63 . 9 ARG HG2 H .98 . 2 64 . 9 ARG HG3 H 1.25 . 2 65 . 9 ARG HD2 H 3.07 . 1 66 . 9 ARG HD3 H 3.07 . 1 67 . 9 ARG HE H 7.02 . 1 68 . 10 GLY H H 8.51 . 1 69 . 10 GLY HA2 H 3.56 . 2 70 . 10 GLY HA3 H 4.15 . 2 71 . 11 ILE H H 7.79 . 1 72 . 11 ILE HA H 4.31 . 1 73 . 11 ILE HB H 1.99 . 1 74 . 11 ILE HG12 H 1.16 . 2 75 . 11 ILE HG13 H 1.5 . 2 76 . 11 ILE HG2 H .77 . 1 77 . 11 ILE HD1 H .88 . 1 78 . 12 CYS H H 8.51 . 1 79 . 12 CYS HA H 5.68 . 1 80 . 12 CYS HB2 H 2.55 . 2 81 . 12 CYS HB3 H 2.95 . 2 82 . 13 TYR H H 9.15 . 1 83 . 13 TYR HA H 4.69 . 1 84 . 13 TYR HB2 H 2.86 . 2 85 . 13 TYR HB3 H 3.04 . 2 86 . 13 TYR HD1 H 7.01 . 1 87 . 13 TYR HD2 H 7.01 . 1 88 . 13 TYR HE1 H 6.71 . 1 89 . 13 TYR HE2 H 6.71 . 1 90 . 14 ARG H H 8.61 . 1 91 . 14 ARG HA H 4.8 . 1 92 . 14 ARG HB2 H 1.66 . 1 93 . 14 ARG HB3 H 1.66 . 1 94 . 14 ARG HG2 H 1.39 . 1 95 . 14 ARG HG3 H 1.39 . 1 96 . 14 ARG HD2 H 3.08 . 1 97 . 14 ARG HD3 H 3.08 . 1 98 . 14 ARG HE H 7.16 . 1 99 . 15 ARG H H 8.71 . 1 100 . 15 ARG HA H 4.55 . 1 101 . 15 ARG HB2 H 1.21 . 2 102 . 15 ARG HB3 H 1.42 . 2 103 . 15 ARG HG2 H 1.25 . 2 104 . 15 ARG HG3 H 1.53 . 2 105 . 15 ARG HD2 H 3.19 . 1 106 . 15 ARG HD3 H 3.19 . 1 107 . 15 ARG HE H 7.15 . 1 108 . 16 CYS H H 8.6 . 1 109 . 16 CYS HA H 5.43 . 1 110 . 16 CYS HB2 H 2.77 . 2 111 . 16 CYS HB3 H 2.99 . 2 stop_ save_