data_1414 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H Resonance Assignment and Secondary Structure Determination of the Dimerization Domain of Transcription Factor LFB1 ; _BMRB_accession_number 1414 _BMRB_flat_file_name bmr1414.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Pastore Annalisa . . 2 'De Francesco' Raffaele . . 3 Barbato Gaetano . . 4 'Castiglione Morelli' Maria Antoinetta . 5 Motta Andrea . . 6 Cortese Riccardo . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 181 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Pastore, Annalisa, De Francesco, Raffaele, Barbato, Gaetano, Castiglione Morelli, Maria Antoinetta, Motta, Andrea, Cortese, Riccardo, "1H Resonance Assignment and Secondary Structure Determination of the Dimerization Domain of Transcription Factor LFB1," Biochemistry 30 (1), 148-153 (1991). ; _Citation_title ; 1H Resonance Assignment and Secondary Structure Determination of the Dimerization Domain of Transcription Factor LFB1 ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Pastore Annalisa . . 2 'De Francesco' Raffaele . . 3 Barbato Gaetano . . 4 'Castiglione Morelli' Maria Antoinetta . 5 Motta Andrea . . 6 Cortese Riccardo . . stop_ _Journal_abbreviation Biochemistry _Journal_volume 30 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 148 _Page_last 153 _Year 1991 _Details . save_ ################################## # Molecular system description # ################################## save_system_transcription_factor_LFB1 _Saveframe_category molecular_system _Mol_system_name 'transcription factor LFB1' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'transcription factor LFB1' $transcription_factor_LFB1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_transcription_factor_LFB1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'transcription factor LFB1' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 32 _Mol_residue_sequence ; MVSKLSQLQTELLAALLESG LSKEALIQALGG ; loop_ _Residue_seq_code _Residue_label 1 MET 2 VAL 3 SER 4 LYS 5 LEU 6 SER 7 GLN 8 LEU 9 GLN 10 THR 11 GLU 12 LEU 13 LEU 14 ALA 15 ALA 16 LEU 17 LEU 18 GLU 19 SER 20 GLY 21 LEU 22 SER 23 LYS 24 GLU 25 ALA 26 LEU 27 ILE 28 GLN 29 ALA 30 LEU 31 GLY 32 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-06-13 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1F93 "Crystal Structure Of A Complex Between The Dimerization Domain Of Hnf-1 Alpha And The Coactivator Dcoh" 96.88 32 100.00 100.00 7.15e-09 PDB 1G39 "Wild-Type Hnf-1alpha Dimerization Domain" 96.88 32 100.00 100.00 7.15e-09 EMBL CAA38295 "unnamed protein product [Rattus sp.]" 96.88 628 100.00 100.00 4.13e-08 EMBL CAB59201 "Hepatocyte nuclear factor 1 [Homo sapiens]" 96.88 500 100.00 100.00 4.49e-08 GB AAA37821 "HNF-3/fork-head homolog-1 [Mus musculus]" 96.88 628 100.00 100.00 4.02e-08 GB AAA41524 "LF-B1 protein [Rattus norvegicus]" 96.88 628 100.00 100.00 4.13e-08 GB AAA88077 "hepatic nuclear factor 1 [Homo sapiens]" 96.88 631 100.00 100.00 4.75e-08 GB AAC51137 "hepatic nuclear factor 1-alpha [Homo sapiens]" 96.88 631 100.00 100.00 4.57e-08 GB AAH80698 "HNF1 homeobox A [Mus musculus]" 96.88 628 100.00 100.00 4.17e-08 PIR S39607 "transcription factor HNF-1A, splice form C - human" 96.88 494 100.00 100.00 5.62e-08 PIR S39608 "transcription factor HNF-1A, splice form B - human" 96.88 542 100.00 100.00 5.30e-08 REF NP_000536 "hepatocyte nuclear factor 1-alpha isoform 2 [Homo sapiens]" 96.88 631 100.00 100.00 4.75e-08 REF NP_001027560 "hepatocyte nuclear factor 1-alpha [Sus scrofa]" 96.88 631 100.00 100.00 4.93e-08 REF NP_001179453 "hepatocyte nuclear factor 1-alpha [Bos taurus]" 96.88 633 100.00 100.00 4.45e-08 REF NP_001293108 "hepatocyte nuclear factor 1-alpha isoform 1 [Homo sapiens]" 96.88 638 100.00 100.00 4.78e-08 REF NP_033353 "hepatocyte nuclear factor 1-alpha [Mus musculus]" 96.88 628 100.00 100.00 4.17e-08 SP P15257 "RecName: Full=Hepatocyte nuclear factor 1-alpha; Short=HNF-1-alpha; Short=HNF-1A; AltName: Full=Liver-specific transcription fa" 96.88 628 100.00 100.00 4.13e-08 SP P20823 "RecName: Full=Hepatocyte nuclear factor 1-alpha; Short=HNF-1-alpha; Short=HNF-1A; AltName: Full=Liver-specific transcription fa" 96.88 631 100.00 100.00 4.75e-08 SP P22361 "RecName: Full=Hepatocyte nuclear factor 1-alpha; Short=HNF-1-alpha; Short=HNF-1A; AltName: Full=Liver-specific transcription fa" 96.88 628 100.00 100.00 4.17e-08 TPG DAA20711 "TPA: HNF1 homeobox A [Bos taurus]" 96.88 633 100.00 100.00 4.45e-08 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $transcription_factor_LFB1 . . . . . . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $transcription_factor_LFB1 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 2.7 . na temperature 299 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis DSS H . . ppm 0 . . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'transcription factor LFB1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 MET HA H 4.18 . 1 2 . 1 MET HB2 H 2.14 . 1 3 . 1 MET HB3 H 2.14 . 1 4 . 1 MET HG2 H 2.55 . 1 5 . 1 MET HG3 H 2.55 . 1 6 . 2 VAL H H 8.57 . 1 7 . 2 VAL HA H 4.15 . 1 8 . 2 VAL HB H 2.04 . 1 9 . 2 VAL HG1 H .94 . 1 10 . 2 VAL HG2 H .94 . 1 11 . 3 SER H H 8.44 . 1 12 . 3 SER HA H 4.43 . 1 13 . 3 SER HB2 H 3.78 . 1 14 . 3 SER HB3 H 3.78 . 1 15 . 4 LYS H H 8.4 . 1 16 . 4 LYS HA H 4.36 . 1 17 . 4 LYS HB2 H 1.81 . 2 18 . 4 LYS HB3 H 1.68 . 2 19 . 4 LYS HG2 H 1.43 . 2 20 . 4 LYS HG3 H 1.38 . 2 21 . 4 LYS HD2 H 1.64 . 1 22 . 4 LYS HD3 H 1.64 . 1 23 . 4 LYS HE2 H 2.97 . 1 24 . 4 LYS HE3 H 2.97 . 1 25 . 4 LYS HZ H 7.52 . 1 26 . 5 LEU H H 8.22 . 1 27 . 5 LEU HA H 4.5 . 1 28 . 5 LEU HB2 H 1.57 . 1 29 . 5 LEU HB3 H 1.57 . 1 30 . 5 LEU HG H 1.62 . 1 31 . 5 LEU HD1 H .72 . 2 32 . 5 LEU HD2 H .66 . 2 33 . 6 SER H H 8.82 . 1 34 . 6 SER HA H 4.48 . 1 35 . 6 SER HB2 H 4.35 . 2 36 . 6 SER HB3 H 3.94 . 2 37 . 7 GLN H H 9.15 . 1 38 . 7 GLN HA H 4.04 . 1 39 . 7 GLN HB2 H 2.1 . 2 40 . 7 GLN HB3 H 1.98 . 2 41 . 7 GLN HG2 H 2.31 . 1 42 . 7 GLN HG3 H 2.31 . 1 43 . 7 GLN HE21 H 7.27 . 2 44 . 7 GLN HE22 H 6.84 . 2 45 . 8 LEU H H 8.38 . 1 46 . 8 LEU HA H 4.09 . 1 47 . 8 LEU HB2 H 1.6 . 1 48 . 8 LEU HB3 H 1.6 . 1 49 . 8 LEU HG H 1.48 . 1 50 . 8 LEU HD1 H .83 . 2 51 . 8 LEU HD2 H .71 . 2 52 . 9 GLN H H 7.68 . 1 53 . 9 GLN HA H 3.63 . 1 54 . 9 GLN HB2 H 2.35 . 2 55 . 9 GLN HB3 H 1.6 . 2 56 . 9 GLN HG2 H 2.53 . 1 57 . 9 GLN HG3 H 2.53 . 1 58 . 9 GLN HE21 H 7.66 . 2 59 . 9 GLN HE22 H 6.7 . 2 60 . 10 THR H H 8.61 . 1 61 . 10 THR HA H 3.7 . 1 62 . 10 THR HB H 4.37 . 1 63 . 10 THR HG2 H 1.21 . 1 64 . 11 GLU H H 8.04 . 1 65 . 11 GLU HA H 4.08 . 1 66 . 11 GLU HB2 H 2.32 . 2 67 . 11 GLU HB3 H 2.1 . 2 68 . 11 GLU HG2 H 2.52 . 1 69 . 11 GLU HG3 H 2.52 . 1 70 . 12 LEU H H 8.2 . 1 71 . 12 LEU HA H 3.94 . 1 72 . 12 LEU HB2 H 1.97 . 2 73 . 12 LEU HB3 H 1.9 . 2 74 . 12 LEU HG H 1.22 . 1 75 . 12 LEU HD1 H .83 . 2 76 . 12 LEU HD2 H .72 . 2 77 . 13 LEU H H 8.32 . 1 78 . 13 LEU HA H 3.97 . 1 79 . 13 LEU HB2 H 1.88 . 2 80 . 13 LEU HB3 H 1.81 . 2 81 . 13 LEU HG H 1.63 . 1 82 . 13 LEU HD1 H .86 . 2 83 . 13 LEU HD2 H .75 . 2 84 . 14 ALA H H 8.03 . 1 85 . 14 ALA HA H 4 . 1 86 . 14 ALA HB H 1.42 . 1 87 . 15 ALA H H 8.04 . 1 88 . 15 ALA HA H 4.08 . 1 89 . 15 ALA HB H 1.42 . 1 90 . 16 LEU H H 8.15 . 1 91 . 16 LEU HA H 3.86 . 1 92 . 16 LEU HB2 H 1.86 . 2 93 . 16 LEU HB3 H 1.72 . 2 94 . 16 LEU HG H 1.57 . 1 95 . 16 LEU HD1 H .86 . 2 96 . 16 LEU HD2 H .75 . 2 97 . 17 LEU H H 8.32 . 1 98 . 17 LEU HA H 4.52 . 1 99 . 17 LEU HB2 H 1.9 . 2 100 . 17 LEU HB3 H 1.77 . 2 101 . 17 LEU HG H 1.58 . 1 102 . 17 LEU HD1 H .86 . 2 103 . 17 LEU HD2 H .75 . 2 104 . 18 GLU H H 8.53 . 1 105 . 18 GLU HA H 4.22 . 1 106 . 18 GLU HB2 H 2.26 . 2 107 . 18 GLU HB3 H 2.18 . 2 108 . 18 GLU HG2 H 2.51 . 1 109 . 18 GLU HG3 H 2.51 . 1 110 . 19 SER H H 8.01 . 1 111 . 19 SER HA H 4.3 . 1 112 . 19 SER HB2 H 4.12 . 2 113 . 19 SER HB3 H 4 . 2 114 . 20 GLY H H 7.9 . 1 115 . 20 GLY HA2 H 3.68 . 2 116 . 20 GLY HA3 H 4.5 . 2 117 . 21 LEU H H 7.02 . 1 118 . 21 LEU HA H 4.51 . 1 119 . 21 LEU HB2 H 1.47 . 2 120 . 21 LEU HB3 H 1.39 . 2 121 . 21 LEU HG H 1.57 . 1 122 . 21 LEU HD1 H .73 . 1 123 . 21 LEU HD2 H .73 . 1 124 . 22 SER H H 8.02 . 1 125 . 22 SER HA H 4.52 . 1 126 . 22 SER HB2 H 4.3 . 2 127 . 22 SER HB3 H 4.03 . 2 128 . 23 LYS H H 8.85 . 1 129 . 23 LYS HA H 3.82 . 1 130 . 23 LYS HB2 H 1.86 . 1 131 . 23 LYS HB3 H 1.86 . 1 132 . 23 LYS HG2 H 1.34 . 1 133 . 23 LYS HG3 H 1.34 . 1 134 . 23 LYS HD2 H 1.76 . 2 135 . 23 LYS HD3 H 1.66 . 2 136 . 23 LYS HE2 H 2.97 . 1 137 . 23 LYS HE3 H 2.97 . 1 138 . 23 LYS HZ H 7.52 . 1 139 . 24 GLU H H 8.36 . 1 140 . 24 GLU HA H 3.97 . 1 141 . 24 GLU HB2 H 2.07 . 2 142 . 24 GLU HB3 H 1.92 . 2 143 . 24 GLU HG2 H 2.53 . 2 144 . 24 GLU HG3 H 2.45 . 2 145 . 25 ALA H H 8 . 1 146 . 25 ALA HA H 4.07 . 1 147 . 25 ALA HB H 1.5 . 1 148 . 26 LEU H H 8.08 . 1 149 . 26 LEU HA H 3.99 . 1 150 . 26 LEU HB2 H 1.74 . 2 151 . 26 LEU HB3 H 1.57 . 2 152 . 26 LEU HD1 H .8 . 2 153 . 26 LEU HD2 H .71 . 2 154 . 27 ILE H H 8.27 . 1 155 . 27 ILE HA H 3.37 . 1 156 . 27 ILE HB H 1.87 . 1 157 . 27 ILE HD1 H .73 . 1 158 . 28 GLN H H 8.05 . 1 159 . 28 GLN HA H 3.99 . 1 160 . 28 GLN HB2 H 2.1 . 1 161 . 28 GLN HB3 H 2.1 . 1 162 . 28 GLN HG2 H 2.5 . 2 163 . 28 GLN HG3 H 2.34 . 2 164 . 28 GLN HE21 H 7.35 . 2 165 . 28 GLN HE22 H 6.75 . 2 166 . 29 ALA H H 8.13 . 1 167 . 29 ALA HA H 4.15 . 1 168 . 29 ALA HB H 1.48 . 1 169 . 30 LEU H H 7.7 . 1 170 . 30 LEU HA H 4.2 . 1 171 . 30 LEU HB2 H 1.86 . 1 172 . 30 LEU HB3 H 1.86 . 1 173 . 30 LEU HG H 1.52 . 1 174 . 30 LEU HD1 H .88 . 2 175 . 30 LEU HD2 H .72 . 2 176 . 31 GLY H H 7.73 . 1 177 . 31 GLY HA2 H 3.85 . 2 178 . 31 GLY HA3 H 4.15 . 2 179 . 32 GLY H H 8.12 . 1 180 . 32 GLY HA2 H 3.68 . 2 181 . 32 GLY HA3 H 4.03 . 2 stop_ save_