data_1517 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Complete assignment of the 1H NMR spectrum and secondary structure of the DNA binding domain of GAL4 ; _BMRB_accession_number 1517 _BMRB_flat_file_name bmr1517.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-04-13 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Gadhavi Paresh L. . 2 Raine Andrew R.C. . 3 Alefounder Peter R. . 4 Laue Ernest D. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 297 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-14 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-04-13 revision BMRB 'Link to the Protein Data Bank added' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Gadhavi, Paresh L., Raine, Andrew R.C., Alefounder, Peter R., Laue, Ernest D., "Complete assignment of the 1H NMR spectrum and secondary structure of the DNA binding domain of GAL4," FEBS Lett. 276 (1), 49-53 (1990). ; _Citation_title ; Complete assignment of the 1H NMR spectrum and secondary structure of the DNA binding domain of GAL4 ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Gadhavi Paresh L. . 2 Raine Andrew R.C. . 3 Alefounder Peter R. . 4 Laue Ernest D. . stop_ _Journal_abbreviation 'FEBS Lett.' _Journal_volume 276 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 49 _Page_last 53 _Year 1990 _Details . save_ ################################## # Molecular system description # ################################## save_system_transcriptional_activator_GAL4 _Saveframe_category molecular_system _Mol_system_name 'transcriptional activator GAL4' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'transcriptional activator GAL4' $transcriptional_activator_GAL4 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_transcriptional_activator_GAL4 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'transcriptional activator GAL4' _Name_variant 'residues 7-49, DNA-binding domain' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 43 _Mol_residue_sequence ; IEQACDICRLKKLKCSKEKP KCAKCLKNNWECRYSPKTKR SPL ; loop_ _Residue_seq_code _Residue_label 1 ILE 2 GLU 3 GLN 4 ALA 5 CYS 6 ASP 7 ILE 8 CYS 9 ARG 10 LEU 11 LYS 12 LYS 13 LEU 14 LYS 15 CYS 16 SER 17 LYS 18 GLU 19 LYS 20 PRO 21 LYS 22 CYS 23 ALA 24 LYS 25 CYS 26 LEU 27 LYS 28 ASN 29 ASN 30 TRP 31 GLU 32 CYS 33 ARG 34 TYR 35 SER 36 PRO 37 LYS 38 THR 39 LYS 40 ARG 41 SER 42 PRO 43 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1620 "transcriptional activator GAL4" 100.00 62 100.00 100.00 1.55e-20 BMRB 1638 "transcriptional activator GAL4" 100.00 43 100.00 100.00 7.25e-20 BMRB 1649 "transcriptional activator GAL4" 100.00 43 100.00 100.00 7.25e-20 BMRB 2051 "transcriptional activator GAL4" 100.00 62 100.00 100.00 1.55e-20 BMRB 2052 "transcriptional activator GAL4" 100.00 62 100.00 100.00 1.55e-20 BMRB 2053 "transcriptional activator GAL4" 100.00 62 100.00 100.00 1.55e-20 PDB 1AW6 "Gal4 (Cd), Nmr, 24 Structures" 86.05 43 100.00 100.00 7.42e-16 PDB 1D66 "Dna Recognition By Gal4: Structure Of A ProteinDNA COMPLEX" 100.00 66 100.00 100.00 1.40e-20 PDB 3COQ "Structural Basis For Dimerization In Dna Recognition By Gal4" 97.67 89 100.00 100.00 4.00e-20 DBJ BAA78208 "Gal4 [Dual-tagging gene trap vector pGT1]" 100.00 881 100.00 100.00 3.68e-22 DBJ BAD35035 "Gal4 [P1-specific P-element Gal4 driver pP{5'P1-Gal4-3'P1}]" 100.00 881 100.00 100.00 3.68e-22 DBJ BAG06943 "DNA binding domain of GAL4 transcriptional activator from yeast [synthetic construct]" 100.00 147 100.00 100.00 2.48e-21 DBJ GAA26731 "K7_Gal4ap [Saccharomyces cerevisiae Kyokai no. 7]" 100.00 658 100.00 100.00 8.29e-20 EMBL CAA59966 "Gal4, aa 1-147 [synthetic construct]" 100.00 147 100.00 100.00 2.48e-21 EMBL CAA91596 "GAL4 [Saccharomyces cerevisiae]" 100.00 881 100.00 100.00 3.68e-22 EMBL CAA97969 "GAL4 [Saccharomyces cerevisiae]" 100.00 881 100.00 100.00 3.68e-22 EMBL CAB54527 "hypothetical protein [Cloning vector pKG1M]" 100.00 177 100.00 100.00 6.07e-21 EMBL CAN84669 "yeast transcription factor [Transposon mutagenesis vector pMiET]" 100.00 881 100.00 100.00 3.68e-22 GB AAA34626 "GAL4 protein [Saccharomyces cerevisiae]" 100.00 881 100.00 100.00 3.68e-22 GB AAA83258 "GAL4, partial [Cloning vector pAS2]" 100.00 147 100.00 100.00 2.48e-21 GB AAA92919 "GAL4, partial [Cloning vector pAS1-tet]" 100.00 147 100.00 100.00 2.48e-21 GB AAA96383 "fusion protein [Cloning vector pAS2-1]" 100.00 187 100.00 100.00 4.95e-21 GB AAB02234 "GAL4 DNA-binding domain, partial [Cloning vector pODB8]" 100.00 147 100.00 100.00 2.48e-21 REF NP_015076 "galactose-responsive transcription factor GAL4 [Saccharomyces cerevisiae S288c]" 100.00 881 100.00 100.00 3.68e-22 SP P04386 "RecName: Full=Regulatory protein GAL4" 100.00 881 100.00 100.00 3.68e-22 TPG DAA11189 "TPA: galactose-responsive transcription factor GAL4 [Saccharomyces cerevisiae S288c]" 100.00 881 100.00 100.00 3.68e-22 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $transcriptional_activator_GAL4 yeast 4932 Eukaryota Fungi Saccharomyces cerevisiae generic stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $transcriptional_activator_GAL4 'not available' . Escherichia coli generic . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.4 . na temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label TSP H . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'transcriptional activator GAL4' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 ILE HA H 3.87 . 1 2 . 1 ILE HB H 1.96 . 1 3 . 1 ILE HG12 H 1.22 . 1 4 . 1 ILE HG13 H 1.22 . 1 5 . 1 ILE HG2 H .98 . 1 6 . 1 ILE HD1 H .91 . 1 7 . 2 GLU H H 8.91 . 1 8 . 2 GLU HA H 4.45 . 1 9 . 2 GLU HB2 H 1.91 . 2 10 . 2 GLU HB3 H 2.1 . 2 11 . 2 GLU HG2 H 2.26 . 2 12 . 2 GLU HG3 H 2.33 . 2 13 . 3 GLN H H 8.69 . 1 14 . 3 GLN HA H 4.51 . 1 15 . 3 GLN HB2 H 2.1 . 2 16 . 3 GLN HB3 H 2.2 . 2 17 . 3 GLN HG2 H 2.56 . 1 18 . 3 GLN HG3 H 2.56 . 1 19 . 3 GLN HE21 H 7.02 . 2 20 . 3 GLN HE22 H 7.77 . 2 21 . 4 ALA H H 8.44 . 1 22 . 4 ALA HA H 4.65 . 1 23 . 4 ALA HB H 1.56 . 1 24 . 5 CYS H H 9.42 . 1 25 . 5 CYS HA H 4.17 . 1 26 . 5 CYS HB2 H 2.21 . 2 27 . 5 CYS HB3 H 2.93 . 2 28 . 6 ASP H H 9.2 . 1 29 . 6 ASP HA H 4.14 . 1 30 . 6 ASP HB2 H 2.63 . 1 31 . 6 ASP HB3 H 2.63 . 1 32 . 7 ILE H H 8.1 . 1 33 . 7 ILE HA H 3.73 . 1 34 . 7 ILE HB H 1.78 . 1 35 . 7 ILE HG12 H .91 . 2 36 . 7 ILE HG13 H 1.55 . 2 37 . 7 ILE HG2 H .68 . 1 38 . 7 ILE HD1 H .68 . 1 39 . 8 CYS H H 7.86 . 1 40 . 8 CYS HA H 3.81 . 1 41 . 8 CYS HB2 H 2.82 . 2 42 . 8 CYS HB3 H 3.27 . 2 43 . 9 ARG H H 8.57 . 1 44 . 9 ARG HA H 4.12 . 1 45 . 9 ARG HB2 H 1.84 . 2 46 . 9 ARG HB3 H 1.96 . 2 47 . 9 ARG HG2 H 1.58 . 1 48 . 9 ARG HG3 H 1.58 . 1 49 . 9 ARG HD2 H 3.21 . 2 50 . 9 ARG HD3 H 3.36 . 2 51 . 9 ARG HE H 7.33 . 1 52 . 10 LEU H H 8.01 . 1 53 . 10 LEU HA H 4.08 . 1 54 . 10 LEU HB2 H 1.77 . 1 55 . 10 LEU HB3 H 1.77 . 1 56 . 10 LEU HG H 1.62 . 1 57 . 10 LEU HD1 H .86 . 1 58 . 10 LEU HD2 H .86 . 1 59 . 11 LYS H H 8.27 . 1 60 . 11 LYS HA H 4.14 . 1 61 . 11 LYS HB2 H 1.52 . 2 62 . 11 LYS HB3 H 1.62 . 2 63 . 11 LYS HG2 H 1.29 . 2 64 . 11 LYS HG3 H 1.35 . 2 65 . 11 LYS HD2 H 1.97 . 1 66 . 11 LYS HD3 H 1.97 . 1 67 . 11 LYS HE2 H 2.37 . 2 68 . 11 LYS HE3 H 2.49 . 2 69 . 12 LYS H H 7.73 . 1 70 . 12 LYS HA H 3.93 . 1 71 . 12 LYS HB2 H 1.88 . 2 72 . 12 LYS HB3 H 2.16 . 2 73 . 12 LYS HG2 H 1.37 . 1 74 . 12 LYS HG3 H 1.37 . 1 75 . 12 LYS HD2 H 1.69 . 2 76 . 12 LYS HD3 H 1.76 . 2 77 . 12 LYS HE2 H 3.04 . 1 78 . 12 LYS HE3 H 3.04 . 1 79 . 12 LYS HZ H 7.59 . 1 80 . 13 LEU H H 8.72 . 1 81 . 13 LEU HA H 4.67 . 1 82 . 13 LEU HB2 H 1.52 . 2 83 . 13 LEU HB3 H 1.86 . 2 84 . 13 LEU HG H 1.6 . 1 85 . 13 LEU HD1 H .9 . 2 86 . 13 LEU HD2 H 1.1 . 2 87 . 14 LYS H H 8.51 . 1 88 . 14 LYS HA H 4.37 . 1 89 . 14 LYS HB2 H 1.77 . 1 90 . 14 LYS HB3 H 1.77 . 1 91 . 14 LYS HG2 H 1.86 . 1 92 . 14 LYS HG3 H 1.86 . 1 93 . 14 LYS HD2 H 1.65 . 1 94 . 14 LYS HD3 H 1.65 . 1 95 . 14 LYS HE2 H 3.21 . 1 96 . 14 LYS HE3 H 3.21 . 1 97 . 14 LYS HZ H 7.25 . 1 98 . 15 CYS H H 8.95 . 1 99 . 15 CYS HA H 4.72 . 1 100 . 15 CYS HB2 H 2.92 . 1 101 . 15 CYS HB3 H 2.92 . 1 102 . 16 SER H H 9.26 . 1 103 . 16 SER HA H 4.52 . 1 104 . 16 SER HB2 H 4.03 . 2 105 . 16 SER HB3 H 4.33 . 2 106 . 17 LYS H H 9.57 . 1 107 . 17 LYS HA H 4.58 . 1 108 . 17 LYS HB2 H 1.97 . 2 109 . 17 LYS HB3 H 2.05 . 2 110 . 17 LYS HG2 H 1.47 . 2 111 . 17 LYS HG3 H 1.43 . 2 112 . 17 LYS HD2 H 1.58 . 1 113 . 17 LYS HD3 H 1.58 . 1 114 . 17 LYS HE2 H 2.92 . 2 115 . 17 LYS HE3 H 3 . 2 116 . 18 GLU H H 7.25 . 1 117 . 18 GLU HA H 4.11 . 1 118 . 18 GLU HB2 H 1.91 . 2 119 . 18 GLU HB3 H 2.05 . 2 120 . 18 GLU HG2 H 2.26 . 2 121 . 18 GLU HG3 H 2.33 . 2 122 . 19 LYS H H 8.52 . 1 123 . 19 LYS HA H 4.54 . 1 124 . 19 LYS HB2 H 1.58 . 2 125 . 19 LYS HB3 H 1.75 . 2 126 . 19 LYS HG2 H 1.33 . 2 127 . 19 LYS HG3 H 1.24 . 2 128 . 19 LYS HD2 H 2.03 . 1 129 . 19 LYS HD3 H 2.03 . 1 130 . 19 LYS HE2 H 2.39 . 1 131 . 19 LYS HE3 H 2.39 . 1 132 . 20 PRO HA H 4.49 . 1 133 . 20 PRO HB2 H 2.23 . 2 134 . 20 PRO HB3 H 2.52 . 2 135 . 20 PRO HG2 H 1.89 . 2 136 . 20 PRO HG3 H 2.08 . 2 137 . 20 PRO HD2 H 3.46 . 2 138 . 20 PRO HD3 H 3.8 . 2 139 . 21 LYS H H 7.92 . 1 140 . 21 LYS HA H 5.49 . 1 141 . 21 LYS HB2 H 1.66 . 1 142 . 21 LYS HB3 H 1.66 . 1 143 . 21 LYS HG2 H 1.565 . 1 144 . 21 LYS HG3 H 1.565 . 1 145 . 21 LYS HD2 H 1.565 . 1 146 . 21 LYS HD3 H 1.565 . 1 147 . 21 LYS HE2 H 3.05 . 2 148 . 21 LYS HE3 H 3.11 . 2 149 . 22 CYS H H 10.1 . 1 150 . 22 CYS HA H 4.67 . 1 151 . 22 CYS HB2 H 3.58 . 2 152 . 22 CYS HB3 H 3.7 . 2 153 . 23 ALA H H 8.37 . 1 154 . 23 ALA HA H 4.03 . 1 155 . 23 ALA HB H 1.52 . 1 156 . 24 LYS H H 8.34 . 1 157 . 24 LYS HA H 3.99 . 1 158 . 24 LYS HB2 H 2.03 . 1 159 . 24 LYS HB3 H 2.03 . 1 160 . 24 LYS HG2 H 1.55 . 2 161 . 24 LYS HG3 H 1.47 . 2 162 . 24 LYS HD2 H 1.7 . 1 163 . 24 LYS HD3 H 1.7 . 1 164 . 24 LYS HE2 H 2.97 . 2 165 . 24 LYS HE3 H 3.07 . 2 166 . 25 CYS H H 8.01 . 1 167 . 25 CYS HA H 4.02 . 1 168 . 25 CYS HB2 H 2.8 . 2 169 . 25 CYS HB3 H 3.32 . 2 170 . 26 LEU H H 8.71 . 1 171 . 26 LEU HA H 4.03 . 1 172 . 26 LEU HB2 H 1.52 . 2 173 . 26 LEU HB3 H 1.78 . 2 174 . 26 LEU HG H 1.52 . 1 175 . 26 LEU HD1 H .91 . 1 176 . 26 LEU HD2 H .91 . 1 177 . 27 LYS H H 7.87 . 1 178 . 27 LYS HA H 3.93 . 1 179 . 27 LYS HB2 H 1.69 . 2 180 . 27 LYS HB3 H 1.84 . 2 181 . 27 LYS HG2 H 1.33 . 1 182 . 27 LYS HG3 H 1.33 . 1 183 . 27 LYS HD2 H 1.58 . 1 184 . 27 LYS HD3 H 1.58 . 1 185 . 27 LYS HE2 H 2.92 . 1 186 . 27 LYS HE3 H 2.92 . 1 187 . 28 ASN H H 7.02 . 1 188 . 28 ASN HA H 4.19 . 1 189 . 28 ASN HB2 H .57 . 2 190 . 28 ASN HB3 H 1.18 . 2 191 . 28 ASN HD21 H 6.48 . 2 192 . 28 ASN HD22 H 7.09 . 2 193 . 29 ASN H H 7.51 . 1 194 . 29 ASN HA H 4.43 . 1 195 . 29 ASN HB2 H 2.68 . 2 196 . 29 ASN HB3 H 3.18 . 2 197 . 29 ASN HD21 H 7.61 . 2 198 . 29 ASN HD22 H 6.85 . 2 199 . 30 TRP H H 8.54 . 1 200 . 30 TRP HA H 5.23 . 1 201 . 30 TRP HB2 H 3.2 . 2 202 . 30 TRP HB3 H 3.4 . 2 203 . 30 TRP HD1 H 7 . 1 204 . 30 TRP HE1 H 10.15 . 1 205 . 30 TRP HE3 H 8.05 . 1 206 . 30 TRP HZ2 H 6.86 . 4 207 . 30 TRP HZ3 H 7.4 . 4 208 . 30 TRP HH2 H 7.07 . 1 209 . 31 GLU H H 8.83 . 1 210 . 31 GLU HA H 4.3 . 1 211 . 31 GLU HB2 H 1.94 . 2 212 . 31 GLU HB3 H 1.98 . 2 213 . 31 GLU HG2 H 2.19 . 2 214 . 31 GLU HG3 H 2.3 . 2 215 . 32 CYS H H 8.25 . 1 216 . 32 CYS HA H 4.41 . 1 217 . 32 CYS HB2 H 2.58 . 2 218 . 32 CYS HB3 H 3.18 . 2 219 . 33 ARG H H 8.15 . 1 220 . 33 ARG HA H 4.74 . 1 221 . 33 ARG HB2 H 1.69 . 2 222 . 33 ARG HB3 H 1.75 . 2 223 . 33 ARG HG2 H 1.53 . 2 224 . 33 ARG HG3 H 1.6 . 2 225 . 33 ARG HD2 H 3.17 . 2 226 . 33 ARG HD3 H 3.25 . 2 227 . 33 ARG HE H 7.31 . 1 228 . 34 TYR H H 9.04 . 1 229 . 34 TYR HA H 4.76 . 1 230 . 34 TYR HB2 H 2.78 . 2 231 . 34 TYR HB3 H 2.81 . 2 232 . 34 TYR HD1 H 6.94 . 1 233 . 34 TYR HD2 H 6.94 . 1 234 . 34 TYR HE1 H 6.9 . 1 235 . 34 TYR HE2 H 6.9 . 1 236 . 35 SER H H 9.13 . 1 237 . 35 SER HA H 4.74 . 1 238 . 35 SER HB2 H 3.87 . 2 239 . 35 SER HB3 H 3.94 . 2 240 . 36 PRO HA H 4.55 . 1 241 . 36 PRO HB2 H 1.97 . 2 242 . 36 PRO HB3 H 2.34 . 2 243 . 36 PRO HG2 H 2.07 . 1 244 . 36 PRO HG3 H 2.07 . 1 245 . 36 PRO HD2 H 3.93 . 2 246 . 36 PRO HD3 H 3.78 . 2 247 . 37 LYS H H 8.65 . 1 248 . 37 LYS HA H 4.31 . 1 249 . 37 LYS HB2 H 1.78 . 2 250 . 37 LYS HB3 H 1.85 . 2 251 . 37 LYS HG2 H 1.47 . 1 252 . 37 LYS HG3 H 1.47 . 1 253 . 37 LYS HD2 H 1.72 . 1 254 . 37 LYS HD3 H 1.72 . 1 255 . 37 LYS HE2 H 3.02 . 1 256 . 37 LYS HE3 H 3.02 . 1 257 . 38 THR H H 8.26 . 1 258 . 38 THR HA H 4.33 . 1 259 . 38 THR HB H 4.21 . 1 260 . 38 THR HG2 H 1.22 . 1 261 . 39 LYS H H 8.51 . 1 262 . 39 LYS HA H 4.33 . 1 263 . 39 LYS HB2 H 1.75 . 2 264 . 39 LYS HB3 H 1.84 . 2 265 . 39 LYS HG2 H 1.46 . 1 266 . 39 LYS HG3 H 1.46 . 1 267 . 39 LYS HD2 H 1.7 . 1 268 . 39 LYS HD3 H 1.7 . 1 269 . 39 LYS HE2 H 3.01 . 1 270 . 39 LYS HE3 H 3.01 . 1 271 . 40 ARG H H 8.42 . 1 272 . 40 ARG HA H 4.33 . 1 273 . 40 ARG HB2 H 1.83 . 2 274 . 40 ARG HB3 H 1.73 . 2 275 . 40 ARG HG2 H 1.63 . 1 276 . 40 ARG HG3 H 1.63 . 1 277 . 40 ARG HD2 H 3.21 . 1 278 . 40 ARG HD3 H 3.21 . 1 279 . 40 ARG HE H 7.29 . 1 280 . 41 SER H H 8.15 . 1 281 . 41 SER HA H 4.56 . 1 282 . 41 SER HB2 H 3.75 . 1 283 . 41 SER HB3 H 3.75 . 1 284 . 42 PRO HA H 4.47 . 1 285 . 42 PRO HB2 H 2.01 . 2 286 . 42 PRO HB3 H 2.29 . 2 287 . 42 PRO HG2 H 2.04 . 1 288 . 42 PRO HG3 H 2.04 . 1 289 . 42 PRO HD2 H 3.84 . 2 290 . 42 PRO HD3 H 3.77 . 2 291 . 43 LEU H H 7.93 . 1 292 . 43 LEU HA H 4.16 . 1 293 . 43 LEU HB2 H 1.58 . 1 294 . 43 LEU HB3 H 1.58 . 1 295 . 43 LEU HG H 1.58 . 1 296 . 43 LEU HD1 H .89 . 2 297 . 43 LEU HD2 H .93 . 2 stop_ save_