data_1997 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H NMR and CD Secondary Structure Analysis of Cell Adhesion Promoting Peptide F-9 from Laminin ; _BMRB_accession_number 1997 _BMRB_flat_file_name bmr1997.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Burke Carl . . 2 Mayo Kevin H. . 3 Skubitz Amy P.N. . 4 Furcht Leo T. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 132 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-16 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Burke, Carl, Mayo, Kevin H., Skubitz, Amy P. N., Furcht, Leo T., "1H NMR and CD Secondary Structure Analysis of Cell Adhesion Promoting Peptide F-9 from Laminin," J. Biol. Chem. 266 (29), 19407-19412 (1991). ; _Citation_title ; 1H NMR and CD Secondary Structure Analysis of Cell Adhesion Promoting Peptide F-9 from Laminin ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Burke Carl . . 2 Mayo Kevin H. . 3 Skubitz Amy P.N. . 4 Furcht Leo T. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 266 _Journal_issue 29 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 19407 _Page_last 19412 _Year 1991 _Details . save_ ################################## # Molecular system description # ################################## save_system_cell_adhesion_promoting_peptide _Saveframe_category molecular_system _Mol_system_name 'cell adhesion promoting peptide' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'cell adhesion promoting peptide' $cell_adhesion_promoting_peptide stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_cell_adhesion_promoting_peptide _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'cell adhesion promoting peptide' _Name_variant 'laminin (641-660), peptide F-9' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 20 _Mol_residue_sequence ; RYVVLPRPVCFEKGMNYTVR ; loop_ _Residue_seq_code _Residue_label 1 ARG 2 TYR 3 VAL 4 VAL 5 LEU 6 PRO 7 ARG 8 PRO 9 VAL 10 CYS 11 PHE 12 GLU 13 LYS 14 GLY 15 MET 16 ASN 17 TYR 18 THR 19 VAL 20 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-11-25 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAE27840 "unnamed protein product [Mus musculus]" 100.00 1834 100.00 100.00 5.46e-05 GB AAA39407 "laminin B1 [Mus musculus]" 100.00 1834 100.00 100.00 5.36e-05 GB AAI50810 "Laminin B1 subunit 1 [Mus musculus]" 100.00 1834 100.00 100.00 5.62e-05 GB ALA21271 "laminin subunit beta-1 [Castor fiber]" 100.00 1693 100.00 100.00 5.21e-05 GB EDL36918 "laminin B1 subunit 1, partial [Mus musculus]" 100.00 1849 100.00 100.00 6.01e-05 GB EDM03247 "laminin, beta 1 (predicted) [Rattus norvegicus]" 100.00 1010 100.00 100.00 2.89e-04 REF NP_032508 "laminin subunit beta-1 [Mus musculus]" 100.00 1834 100.00 100.00 5.67e-05 REF XP_003750185 "PREDICTED: laminin subunit beta-1 isoform X2 [Rattus norvegicus]" 100.00 1834 100.00 100.00 6.01e-05 REF XP_004598639 "PREDICTED: laminin subunit beta-1 [Ochotona princeps]" 100.00 1786 100.00 100.00 4.90e-05 REF XP_005076286 "PREDICTED: laminin subunit beta-1 [Mesocricetus auratus]" 100.00 1834 100.00 100.00 5.46e-05 REF XP_005319496 "PREDICTED: laminin subunit beta-1 [Ictidomys tridecemlineatus]" 100.00 1785 100.00 100.00 4.37e-05 SP P02469 "RecName: Full=Laminin subunit beta-1; AltName: Full=Laminin B1 chain; AltName: Full=Laminin-1 subunit beta; AltName: Full=Lamin" 100.00 1786 100.00 100.00 4.86e-05 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $cell_adhesion_promoting_peptide human 9606 Eukaryota Metazoa Homo sapiens generic stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $cell_adhesion_promoting_peptide 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 3.5 . na temperature 288 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label TSP H . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'cell adhesion promoting peptide' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 ARG HA H 4.02 . 1 2 . 1 ARG HB2 H 1.91 . 1 3 . 1 ARG HB3 H 1.91 . 1 4 . 1 ARG HG2 H 1.6 . 1 5 . 1 ARG HG3 H 1.6 . 1 6 . 1 ARG HD2 H 3.2 . 2 7 . 1 ARG HD3 H 3.21 . 2 8 . 1 ARG HE H 7.21 . 1 9 . 2 TYR H H 8.76 . 1 10 . 2 TYR HA H 4.67 . 1 11 . 2 TYR HB2 H 3 . 1 12 . 2 TYR HB3 H 3 . 1 13 . 2 TYR HD1 H 6.79 . 1 14 . 2 TYR HD2 H 6.79 . 1 15 . 2 TYR HE1 H 7.1 . 1 16 . 2 TYR HE2 H 7.1 . 1 17 . 3 VAL H H 8.16 . 1 18 . 3 VAL HA H 4.02 . 1 19 . 3 VAL HB H 2.02 . 1 20 . 3 VAL HG1 H .85 . 2 21 . 3 VAL HG2 H .88 . 2 22 . 4 VAL H H 8.21 . 1 23 . 4 VAL HA H 3.96 . 1 24 . 4 VAL HB H 1.98 . 1 25 . 4 VAL HG1 H .9 . 2 26 . 4 VAL HG2 H .95 . 2 27 . 5 LEU H H 8.4 . 1 28 . 5 LEU HA H 4.61 . 1 29 . 5 LEU HB2 H 1.54 . 2 30 . 5 LEU HB3 H 1.6 . 2 31 . 5 LEU HG H 1.6 . 1 32 . 5 LEU HD1 H .88 . 2 33 . 5 LEU HD2 H .94 . 2 34 . 6 PRO HA H 4.4 . 1 35 . 6 PRO HB2 H 2.27 . 1 36 . 6 PRO HB3 H 2.27 . 1 37 . 6 PRO HG2 H 1.88 . 1 38 . 6 PRO HG3 H 1.88 . 1 39 . 6 PRO HD2 H 3.66 . 2 40 . 6 PRO HD3 H 3.84 . 2 41 . 7 ARG H H 8.36 . 1 42 . 7 ARG HA H 4.58 . 1 43 . 7 ARG HB2 H 1.7 . 1 44 . 7 ARG HB3 H 1.7 . 1 45 . 7 ARG HG2 H 1.72 . 1 46 . 7 ARG HG3 H 1.72 . 1 47 . 7 ARG HD2 H 3.19 . 1 48 . 7 ARG HD3 H 3.19 . 1 49 . 7 ARG HE H 7.16 . 1 50 . 8 PRO HA H 4.44 . 1 51 . 8 PRO HB2 H 2.31 . 1 52 . 8 PRO HB3 H 2.31 . 1 53 . 8 PRO HG2 H 1.88 . 1 54 . 8 PRO HG3 H 1.88 . 1 55 . 8 PRO HD2 H 3.64 . 2 56 . 8 PRO HD3 H 3.84 . 2 57 . 9 VAL H H 8.31 . 1 58 . 9 VAL HA H 4.01 . 1 59 . 9 VAL HB H 1.9 . 1 60 . 9 VAL HG1 H .84 . 2 61 . 9 VAL HG2 H .94 . 2 62 . 10 CYS H H 8.33 . 1 63 . 10 CYS HA H 4.47 . 1 64 . 10 CYS HB2 H 2.84 . 2 65 . 10 CYS HB3 H 2.82 . 2 66 . 11 PHE H H 8.38 . 1 67 . 11 PHE HA H 4.61 . 1 68 . 11 PHE HB2 H 3.02 . 2 69 . 11 PHE HB3 H 3.11 . 2 70 . 11 PHE HD1 H 7.21 . 1 71 . 11 PHE HD2 H 7.21 . 1 72 . 11 PHE HE1 H 7.31 . 1 73 . 11 PHE HE2 H 7.31 . 1 74 . 11 PHE HZ H 7.25 . 1 75 . 12 GLU H H 8.19 . 1 76 . 12 GLU HA H 4.31 . 1 77 . 12 GLU HB2 H 1.9 . 2 78 . 12 GLU HB3 H 2.04 . 2 79 . 12 GLU HG2 H 2.37 . 2 80 . 12 GLU HG3 H 2.39 . 2 81 . 13 LYS H H 8.36 . 1 82 . 13 LYS HA H 4.18 . 1 83 . 13 LYS HB2 H 1.78 . 2 84 . 13 LYS HB3 H 1.82 . 2 85 . 13 LYS HG2 H 1.41 . 2 86 . 13 LYS HG3 H 1.45 . 2 87 . 13 LYS HD2 H 1.67 . 2 88 . 13 LYS HD3 H 1.68 . 2 89 . 13 LYS HE2 H 2.98 . 2 90 . 13 LYS HE3 H 3 . 2 91 . 13 LYS HZ H 6.96 . 1 92 . 14 GLY H H 8.49 . 1 93 . 14 GLY HA2 H 3.89 . 2 94 . 14 GLY HA3 H 4 . 2 95 . 15 MET H H 8.13 . 1 96 . 15 MET HA H 4.43 . 1 97 . 15 MET HB2 H 1.92 . 2 98 . 15 MET HB3 H 1.96 . 2 99 . 15 MET HG2 H 2.45 . 2 100 . 15 MET HG3 H 2.49 . 2 101 . 16 ASN H H 8.47 . 1 102 . 16 ASN HA H 4.68 . 1 103 . 16 ASN HB2 H 2.68 . 2 104 . 16 ASN HB3 H 2.78 . 2 105 . 16 ASN HD21 H 7.6 . 2 106 . 16 ASN HD22 H 6.91 . 2 107 . 17 TYR H H 8.13 . 1 108 . 17 TYR HA H 4.6 . 1 109 . 17 TYR HB2 H 2.94 . 2 110 . 17 TYR HB3 H 3.08 . 2 111 . 17 TYR HD1 H 7.08 . 1 112 . 17 TYR HD2 H 7.08 . 1 113 . 17 TYR HE1 H 6.79 . 1 114 . 17 TYR HE2 H 6.79 . 1 115 . 18 THR H H 8.11 . 1 116 . 18 THR HA H 4.3 . 1 117 . 18 THR HB H 4.09 . 1 118 . 18 THR HG2 H 1.14 . 1 119 . 19 VAL H H 8.17 . 1 120 . 19 VAL HA H 4.08 . 1 121 . 19 VAL HB H 2.06 . 1 122 . 19 VAL HG1 H .96 . 1 123 . 19 VAL HG2 H .96 . 1 124 . 20 ARG H H 8.35 . 1 125 . 20 ARG HA H 4.34 . 1 126 . 20 ARG HB2 H 1.76 . 2 127 . 20 ARG HB3 H 1.91 . 2 128 . 20 ARG HG2 H 1.61 . 2 129 . 20 ARG HG3 H 1.64 . 2 130 . 20 ARG HD2 H 3.19 . 2 131 . 20 ARG HD3 H 3.2 . 2 132 . 20 ARG HE H 7.14 . 1 stop_ save_