data_2053 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structure of the Binuclear Metal-Binding Site in the GAL4 Transcription Factor ; _BMRB_accession_number 2053 _BMRB_flat_file_name bmr2053.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Gardner Kevin H. . 2 Pan Tao . . 3 Narula Surinder S. . 4 Rivera Edwin . . 5 Coleman Joseph E. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 18 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Gardner, Kevin H., Pan, Tao, Narula, Surinder S., Rivera, Edwin, Coleman, Joseph E., "Structure of the Binuclear Metal-Binding Site in the GAL4 Transcription Factor," Biochemistry 30 (47), 11292-11302 (1991). ; _Citation_title 'Structure of the Binuclear Metal-Binding Site in the GAL4 Transcription Factor' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Gardner Kevin H. . 2 Pan Tao . . 3 Narula Surinder S. . 4 Rivera Edwin . . 5 Coleman Joseph E. . stop_ _Journal_abbreviation Biochemistry _Journal_volume 30 _Journal_issue 47 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 11292 _Page_last 11302 _Year 1991 _Details . save_ ################################## # Molecular system description # ################################## save_system_transcriptional_activator_GAL4 _Saveframe_category molecular_system _Mol_system_name 'transcriptional activator GAL4' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'transcriptional activator GAL4' $transcriptional_activator_GAL4 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_transcriptional_activator_GAL4 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'transcriptional activator GAL4' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 62 _Mol_residue_sequence ; MKLLSSIEQACDICRLKKLK CSKEKPKCAKCLKNNWECRY SPKTKRSPLTRAHLTEVESR LD ; loop_ _Residue_seq_code _Residue_label 1 MET 2 LYS 3 LEU 4 LEU 5 SER 6 SER 7 ILE 8 GLU 9 GLN 10 ALA 11 CYS 12 ASP 13 ILE 14 CYS 15 ARG 16 LEU 17 LYS 18 LYS 19 LEU 20 LYS 21 CYS 22 SER 23 LYS 24 GLU 25 LYS 26 PRO 27 LYS 28 CYS 29 ALA 30 LYS 31 CYS 32 LEU 33 LYS 34 ASN 35 ASN 36 TRP 37 GLU 38 CYS 39 ARG 40 TYR 41 SER 42 PRO 43 LYS 44 THR 45 LYS 46 ARG 47 SER 48 PRO 49 LEU 50 THR 51 ARG 52 ALA 53 HIS 54 LEU 55 THR 56 GLU 57 VAL 58 GLU 59 SER 60 ARG 61 LEU 62 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-08-05 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1517 "transcriptional activator GAL4" 69.35 43 100.00 100.00 2.50e-20 BMRB 1620 "transcriptional activator GAL4" 100.00 62 100.00 100.00 2.13e-34 BMRB 1638 "transcriptional activator GAL4" 69.35 43 100.00 100.00 2.50e-20 BMRB 1649 "transcriptional activator GAL4" 69.35 43 100.00 100.00 2.50e-20 BMRB 2051 "transcriptional activator GAL4" 100.00 62 100.00 100.00 2.13e-34 BMRB 2052 "transcriptional activator GAL4" 100.00 62 100.00 100.00 2.13e-34 PDB 1AW6 "Gal4 (Cd), Nmr, 24 Structures" 69.35 43 100.00 100.00 5.87e-20 PDB 1D66 "Dna Recognition By Gal4: Structure Of A ProteinDNA COMPLEX" 100.00 66 98.39 100.00 9.87e-34 PDB 3COQ "Structural Basis For Dimerization In Dna Recognition By Gal4" 88.71 89 98.18 100.00 2.01e-29 DBJ BAA78208 "Gal4 [Dual-tagging gene trap vector pGT1]" 100.00 881 98.39 100.00 3.97e-32 DBJ BAD35035 "Gal4 [P1-specific P-element Gal4 driver pP{5'P1-Gal4-3'P1}]" 100.00 881 98.39 100.00 3.97e-32 DBJ BAG06943 "DNA binding domain of GAL4 transcriptional activator from yeast [synthetic construct]" 100.00 147 98.39 100.00 1.50e-34 DBJ GAA26731 "K7_Gal4ap [Saccharomyces cerevisiae Kyokai no. 7]" 100.00 658 98.39 100.00 3.25e-32 EMBL CAA59966 "Gal4, aa 1-147 [synthetic construct]" 100.00 147 98.39 100.00 1.50e-34 EMBL CAA91596 "GAL4 [Saccharomyces cerevisiae]" 100.00 881 98.39 100.00 3.97e-32 EMBL CAA97969 "GAL4 [Saccharomyces cerevisiae]" 100.00 881 98.39 100.00 3.97e-32 EMBL CAB54527 "hypothetical protein [Cloning vector pKG1M]" 100.00 177 98.39 100.00 3.56e-34 EMBL CAN84669 "yeast transcription factor [Transposon mutagenesis vector pMiET]" 100.00 881 98.39 100.00 3.97e-32 GB AAA34626 "GAL4 protein [Saccharomyces cerevisiae]" 100.00 881 98.39 100.00 3.97e-32 GB AAA83258 "GAL4, partial [Cloning vector pAS2]" 100.00 147 98.39 100.00 1.50e-34 GB AAA92919 "GAL4, partial [Cloning vector pAS1-tet]" 100.00 147 98.39 100.00 1.50e-34 GB AAA96383 "fusion protein [Cloning vector pAS2-1]" 100.00 187 98.39 100.00 3.45e-34 GB AAB02234 "GAL4 DNA-binding domain, partial [Cloning vector pODB8]" 100.00 147 98.39 100.00 1.50e-34 REF NP_015076 "galactose-responsive transcription factor GAL4 [Saccharomyces cerevisiae S288c]" 100.00 881 98.39 100.00 3.97e-32 SP P04386 "RecName: Full=Regulatory protein GAL4" 100.00 881 98.39 100.00 3.97e-32 TPG DAA11189 "TPA: galactose-responsive transcription factor GAL4 [Saccharomyces cerevisiae S288c]" 100.00 881 98.39 100.00 3.97e-32 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $transcriptional_activator_GAL4 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.4 . na temperature 308 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis TSP H . . ppm 0 . . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'transcriptional activator GAL4' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 11 CYS HA H 4.12 . 1 2 . 11 CYS HB2 H 2.88 . 2 3 . 11 CYS HB3 H 2.15 . 2 4 . 14 CYS HA H 3.73 . 1 5 . 14 CYS HB2 H 3.17 . 2 6 . 14 CYS HB3 H 2.73 . 2 7 . 21 CYS HA H 4.65 . 1 8 . 21 CYS HB2 H 2.84 . 1 9 . 21 CYS HB3 H 2.84 . 1 10 . 28 CYS HA H 4.67 . 1 11 . 28 CYS HB2 H 3.6 . 2 12 . 28 CYS HB3 H 3.5 . 2 13 . 31 CYS HA H 3.98 . 1 14 . 31 CYS HB2 H 3.25 . 2 15 . 31 CYS HB3 H 2.75 . 2 16 . 38 CYS HA H 4.29 . 1 17 . 38 CYS HB2 H 3.04 . 2 18 . 38 CYS HB3 H 2.55 . 2 stop_ save_