data_21 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural and dynamical comparison of alpha, beta, and gamma forms of murine epidermal growth factor ; _BMRB_accession_number 21 _BMRB_flat_file_name bmr21.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-04-13 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Mayo Kevin H. . 2 Burke Carl . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 35 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-09 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-04-13 revision BMRB 'Link to the Protein Data Bank added' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Mayo, Kevin H., Burke, Carl, "Structural and dynamical comparison of alpha, beta, and gamma forms of murine epidermal growth factor," Eur. J. Biochem. 169, 201-207 (1987). ; _Citation_title ; Structural and dynamical comparison of alpha, beta, and gamma forms of murine epidermal growth factor ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Mayo Kevin H. . 2 Burke Carl . . stop_ _Journal_abbreviation 'Eur. J. Biochem.' _Journal_volume 169 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 201 _Page_last 207 _Year 1987 _Details . save_ ################################## # Molecular system description # ################################## save_system_epidermal_growth_factor _Saveframe_category molecular_system _Mol_system_name 'epidermal growth factor' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'epidermal growth factor' $epidermal_growth_factor stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_epidermal_growth_factor _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'epidermal growth factor' _Name_variant alpha _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 53 _Mol_residue_sequence ; NSYPGCPSSYDGYCLNGGVC MHIESLDSYTCNCVIGYSGD RCQTRDLRWWELR ; loop_ _Residue_seq_code _Residue_label 1 ASN 2 SER 3 TYR 4 PRO 5 GLY 6 CYS 7 PRO 8 SER 9 SER 10 TYR 11 ASP 12 GLY 13 TYR 14 CYS 15 LEU 16 ASN 17 GLY 18 GLY 19 VAL 20 CYS 21 MET 22 HIS 23 ILE 24 GLU 25 SER 26 LEU 27 ASP 28 SER 29 TYR 30 THR 31 CYS 32 ASN 33 CYS 34 VAL 35 ILE 36 GLY 37 TYR 38 SER 39 GLY 40 ASP 41 ARG 42 CYS 43 GLN 44 THR 45 ARG 46 ASP 47 LEU 48 ARG 49 TRP 50 TRP 51 GLU 52 LEU 53 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-08-05 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1674 "epidermal growth factor" 100.00 53 100.00 100.00 5.88e-30 BMRB 1729 "epidermal growth factor" 100.00 53 100.00 100.00 5.88e-30 BMRB 2201 "epidermal growth factor" 100.00 53 100.00 100.00 5.88e-30 BMRB 2249 "epidermal growth factor" 100.00 53 100.00 100.00 5.88e-30 PDB 1EGF "Solution Structure Of Murine Epidermal Growth Factor Determined By Nmr Spectroscopy And Refined By Energy Minimization With Res" 100.00 53 100.00 100.00 5.88e-30 PDB 1EPG "Three-Dimensional Nuclear Magnetic Resonance Structures Of Mouse Epidermal Growth Factor In Acidic And Physiological Ph Solutio" 100.00 53 100.00 100.00 5.88e-30 PDB 1EPH "Three-Dimensional Nuclear Magnetic Resonance Structures Of Mouse Epidermal Growth Factor In Acidic And Physiological Ph Solutio" 100.00 53 100.00 100.00 5.88e-30 PDB 1EPI "Three-Dimensional Nuclear Magnetic Resonance Structures Of Mouse Epidermal Growth Factor In Acidic And Physiological Ph Solutio" 100.00 53 100.00 100.00 5.88e-30 PDB 1EPJ "Three-Dimensional Nuclear Magnetic Resonance Structures Of Mouse Epidermal Growth Factor In Acidic And Physiological Ph Solutio" 100.00 53 100.00 100.00 5.88e-30 PDB 3EGF "Solution Structure Of Murine Epidermal Growth Factor Determined By Nmr Spectroscopy And Refined By Energy Minimization With Res" 100.00 53 100.00 100.00 5.88e-30 DBJ BAE22978 "unnamed protein product [Mus musculus]" 100.00 716 100.00 100.00 3.79e-28 EMBL CAA24115 "unnamed protein product [Mus musculus]" 100.00 1217 100.00 100.00 6.99e-28 EMBL CAA24116 "unnamed protein product [Mus musculus]" 67.92 36 100.00 100.00 6.96e-17 GB AAA37539 "prepro-egf [Mus musculus]" 100.00 1217 100.00 100.00 6.79e-28 GB AAA72791 "epidermal growth factor protein, partial [synthetic construct]" 100.00 56 100.00 100.00 3.70e-30 GB AAA72815 "epidermal growth factor, partial [synthetic construct]" 100.00 54 100.00 100.00 5.46e-30 GB AAH17681 "Egf protein [Mus musculus]" 100.00 1200 100.00 100.00 6.46e-28 GB AAH60741 "Epidermal growth factor [Mus musculus]" 100.00 1217 100.00 100.00 6.99e-28 PRF 0907234A "epidermal growth factor precursor" 100.00 1168 100.00 100.00 5.91e-28 REF NP_001297666 "pro-epidermal growth factor isoform 2 [Mus musculus]" 100.00 716 100.00 100.00 3.79e-28 REF NP_034243 "pro-epidermal growth factor isoform 1 precursor [Mus musculus]" 100.00 1217 100.00 100.00 6.86e-28 REF XP_006501053 "PREDICTED: pro-epidermal growth factor isoform X4 [Mus musculus]" 100.00 1176 100.00 100.00 5.39e-28 REF XP_011238312 "PREDICTED: pro-epidermal growth factor isoform X1 [Mus musculus]" 100.00 1221 100.00 100.00 6.81e-28 REF XP_011238313 "PREDICTED: pro-epidermal growth factor isoform X2 [Mus musculus]" 100.00 1180 100.00 100.00 5.35e-28 SP P01132 "RecName: Full=Pro-epidermal growth factor; Short=EGF; Contains: RecName: Full=Epidermal growth factor; Flags: Precursor" 100.00 1217 100.00 100.00 6.86e-28 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain _Tissue $epidermal_growth_factor mouse 10090 Eukaryota Metazoa Mus musculus generic 'salivary gland' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $epidermal_growth_factor 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 3.5 . na temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'epidermal growth factor' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 3 TYR HD1 H 6.98 . 1 2 . 3 TYR HD2 H 6.98 . 1 3 . 3 TYR HE1 H 6.65 . 1 4 . 3 TYR HE2 H 6.65 . 1 5 . 10 TYR HD1 H 6.27 . 3 6 . 10 TYR HD2 H 6.9 . 3 7 . 10 TYR HE1 H 6.61 . 3 8 . 10 TYR HE2 H 5.81 . 3 9 . 13 TYR HD1 H 6.9 . 3 10 . 13 TYR HD2 H 6.27 . 3 11 . 13 TYR HE1 H 6.61 . 3 12 . 13 TYR HE2 H 5.81 . 3 13 . 15 LEU H H 8.14 . 1 14 . 18 GLY H H 7.3 . 1 15 . 19 VAL H H 7.94 . 1 16 . 21 MET H H 9.4 . 1 17 . 22 HIS HD2 H 6.41 . 1 18 . 22 HIS HE1 H 8.41 . 1 19 . 29 TYR HD1 H 6.63 . 1 20 . 29 TYR HD2 H 6.63 . 1 21 . 29 TYR HE1 H 6.09 . 1 22 . 29 TYR HE2 H 6.09 . 1 23 . 30 THR H H 8.83 . 1 24 . 32 ASN H H 9.24 . 1 25 . 34 VAL H H 8.2 . 1 26 . 35 ILE H H 7.76 . 1 27 . 37 TYR H H 8.03 . 1 28 . 37 TYR HD1 H 6.71 . 1 29 . 37 TYR HD2 H 6.71 . 1 30 . 37 TYR HE1 H 6.48 . 1 31 . 37 TYR HE2 H 6.48 . 1 32 . 38 SER H H 9.17 . 1 33 . 42 CYS H H 7.61 . 1 34 . 43 GLN H H 9.82 . 1 35 . 44 THR H H 8.67 . 1 stop_ save_