data_350 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Kringle-2 domain of the tissue-type plasminogen activator ; _BMRB_accession_number 350 _BMRB_flat_file_name bmr350.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-04-13 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Byeon In-Ja L. . 2 Kelley Robert F. . 3 Llinas M. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-11 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-04-13 revision BMRB 'Link to the Protein Data Bank added' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Byeon, In-Ja L., Kelley, Robert F., Llinas, M., "Kringle-2 domain of the tissue-type plasminogen activator," Eur. J. Biochem. 197, 155-165 (1991). ; _Citation_title 'Kringle-2 domain of the tissue-type plasminogen activator' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Byeon In-Ja L. . 2 Kelley Robert F. . 3 Llinas M. . . stop_ _Journal_abbreviation 'Eur. J. Biochem.' _Journal_volume 197 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 155 _Page_last 165 _Year 1991 _Details . save_ ################################## # Molecular system description # ################################## save_system_tissue_plasminogen_activator _Saveframe_category molecular_system _Mol_system_name 'tissue plasminogen activator' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'tissue plasminogen activator' $tissue_plasminogen_activator stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_tissue_plasminogen_activator _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'tissue plasminogen activator' _Name_variant 'kringle 2 domain' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 90 _Mol_residue_sequence ; SEGNSDCYFGNGSAYRGTHS LTESGASCLPWNSMILIGKV YTAQNPSAQALGLGKHNYCR NPDGDAKPWCHVLKNRRLTW EYCDVPSCST ; loop_ _Residue_seq_code _Residue_label 1 SER 2 GLU 3 GLY 4 ASN 5 SER 6 ASP 7 CYS 8 TYR 9 PHE 10 GLY 11 ASN 12 GLY 13 SER 14 ALA 15 TYR 16 ARG 17 GLY 18 THR 19 HIS 20 SER 21 LEU 22 THR 23 GLU 24 SER 25 GLY 26 ALA 27 SER 28 CYS 29 LEU 30 PRO 31 TRP 32 ASN 33 SER 34 MET 35 ILE 36 LEU 37 ILE 38 GLY 39 LYS 40 VAL 41 TYR 42 THR 43 ALA 44 GLN 45 ASN 46 PRO 47 SER 48 ALA 49 GLN 50 ALA 51 LEU 52 GLY 53 LEU 54 GLY 55 LYS 56 HIS 57 ASN 58 TYR 59 CYS 60 ARG 61 ASN 62 PRO 63 ASP 64 GLY 65 ASP 66 ALA 67 LYS 68 PRO 69 TRP 70 CYS 71 HIS 72 VAL 73 LEU 74 LYS 75 ASN 76 ARG 77 ARG 78 LEU 79 THR 80 TRP 81 GLU 82 TYR 83 CYS 84 ASP 85 VAL 86 PRO 87 SER 88 CYS 89 SER 90 THR stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-29 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1476 "tissue plasminogen activator" 100.00 90 100.00 100.00 5.96e-60 BMRB 1477 "tissue plasminogen activator" 100.00 90 100.00 100.00 5.96e-60 BMRB 2261 "t-PA kringle 2" 94.44 85 100.00 100.00 2.04e-56 BMRB 349 "tissue plasminogen activator" 100.00 90 100.00 100.00 5.96e-60 BMRB 351 "tissue plasminogen activator" 100.00 90 100.00 100.00 5.96e-60 PDB 1PK2 "Solution Structure Of The Tissue-Type Plasminogen Activator Kringle 2 Domain Complexed To 6-Aminohexanoic Acid An Antifibrinoly" 100.00 90 100.00 100.00 5.96e-60 PDB 1PML "Kringle-Kringle Interactions In Multimer Kringle Structures" 95.56 86 100.00 100.00 5.32e-57 PDB 1TPK "Crystal Structure Of The Kringle-2 Domain Of Tissue Plasminogen Activator At 2.4-Angstroms Resolution" 97.78 88 100.00 100.00 1.92e-58 DBJ BAG60088 "unnamed protein product [Homo sapiens]" 97.78 473 100.00 100.00 6.82e-55 DBJ BAG60253 "unnamed protein product [Homo sapiens]" 96.67 436 100.00 100.00 1.36e-54 DBJ BAG62604 "unnamed protein product [Homo sapiens]" 100.00 499 100.00 100.00 4.87e-56 EMBL CAA23833 "unnamed protein product [Homo sapiens]" 55.56 110 100.00 100.00 1.52e-28 GB AAB32090 "tissue plasminogen activator [synthetic construct]" 100.00 92 97.78 98.89 4.48e-57 GB AAK11956 "neonatal thrombolytic agent alpha-form [Homo sapiens]" 100.00 395 100.00 100.00 8.46e-57 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $tissue_plasminogen_activator Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $tissue_plasminogen_activator 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 4.9 . na temperature 336 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label TSP H . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'tissue plasminogen activator' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 17 GLY H H 4.52 . 1 stop_ save_