data_5084 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H and 15N Chemical Shift Assignments for the Complex of C-terminal Domain of Human Poly(A)-Binding Protein and 22-residue Fragment of Paip1 ; _BMRB_accession_number 5084 _BMRB_flat_file_name bmr5084.str _Entry_type original _Submission_date 2001-07-18 _Accession_date 2001-07-23 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kozlov G. . . 2 Siddiqui N. . . 3 Coillet-Matillon S. . . 4 Ekiel I. . . 5 Gehring K. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 coupling_constants 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 406 "15N chemical shifts" 101 "coupling constants" 18 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2003-12-08 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 4915 'non-liganded C-terminal domain of PABP' 5085 'PABC-Paip2 complex' stop_ _Original_release_date 2003-12-08 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structure and function of the C-terminal PABC domain of human poly(A)-binding protein ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 11287632 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kozlov G. . . 2 Trempe J. F. . 3 Khaleghpour K. . . 4 Kahvejian A. . . 5 Ekiel I. . . 6 Gehring K. . . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U. S. A.' _Journal_volume 98 _Journal_issue 8 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 4409 _Page_last 4413 _Year 2001 _Details . loop_ _Keyword 'all-helical domain' 'protein-peptide complex' stop_ save_ ################################## # Molecular system description # ################################## save_system_PABC-Paip1_complex _Saveframe_category molecular_system _Mol_system_name 'PABC-Paip1 complex' _Abbreviation_common 'PABC-Paip1 complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'polyadenylate-binding protein 1 (544-636)' $PABC 'polyadenylate-binding protein-interacting protein-1 (126-147)' $Paip1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_PABC _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'C-terminal domain of PABP' _Abbreviation_common PABC _Molecular_mass 10337 _Mol_thiol_state 'not present' _Details 'N-terminal sequence GPLGS is a cloning artifact' ############################## # Polymer residue sequence # ############################## _Residue_count 98 _Mol_residue_sequence ; GPLGSPLTASMLASAPPQEQ KQMLGERLFPLIQAMHPTLA GKITGMLLEIDNSELLHMLE SPESLRSKVDEAVAVLQAHQ AKEAAQKAVNSATGVPTV ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 PRO 3 LEU 4 GLY 5 SER 6 PRO 7 LEU 8 THR 9 ALA 10 SER 11 MET 12 LEU 13 ALA 14 SER 15 ALA 16 PRO 17 PRO 18 GLN 19 GLU 20 GLN 21 LYS 22 GLN 23 MET 24 LEU 25 GLY 26 GLU 27 ARG 28 LEU 29 PHE 30 PRO 31 LEU 32 ILE 33 GLN 34 ALA 35 MET 36 HIS 37 PRO 38 THR 39 LEU 40 ALA 41 GLY 42 LYS 43 ILE 44 THR 45 GLY 46 MET 47 LEU 48 LEU 49 GLU 50 ILE 51 ASP 52 ASN 53 SER 54 GLU 55 LEU 56 LEU 57 HIS 58 MET 59 LEU 60 GLU 61 SER 62 PRO 63 GLU 64 SER 65 LEU 66 ARG 67 SER 68 LYS 69 VAL 70 ASP 71 GLU 72 ALA 73 VAL 74 ALA 75 VAL 76 LEU 77 GLN 78 ALA 79 HIS 80 GLN 81 ALA 82 LYS 83 GLU 84 ALA 85 ALA 86 GLN 87 LYS 88 ALA 89 VAL 90 ASN 91 SER 92 ALA 93 THR 94 GLY 95 VAL 96 PRO 97 THR 98 VAL stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1JH4 "Solution Structure Of The C-Terminal Pabc Domain Of Human Poly(A)-Binding Protein In Complex With The Peptide From Paip1" 95.45 22 100.00 100.00 3.74e-04 PDB 3NTW "Structure Of The Mlle Domain Of Edd In Complex With A Pam2 Peptide From Paip1" 100.00 22 100.00 100.00 6.74e-05 EMBL CAB66854 "hypothetical protein [Homo sapiens]" 100.00 479 100.00 100.00 3.23e-04 GB AAC39697 "polyadenylate binding protein-interacting protein-1 [Homo sapiens]" 100.00 479 100.00 100.00 3.23e-04 GB AAH05295 "Poly(A) binding protein interacting protein 1 [Homo sapiens]" 100.00 400 100.00 100.00 1.67e-04 GB AAH15937 "Poly(A) binding protein interacting protein 1 [Homo sapiens]" 100.00 479 100.00 100.00 3.20e-04 GB ABM82036 "poly(A) binding protein interacting protein 1 [synthetic construct]" 100.00 479 100.00 100.00 3.20e-04 GB ABW03359 "poly(A) binding protein interacting protein 1 [synthetic construct]" 100.00 479 100.00 100.00 3.20e-04 REF NP_006442 "polyadenylate-binding protein-interacting protein 1 isoform 1 [Homo sapiens]" 100.00 479 100.00 100.00 3.23e-04 REF NP_877590 "polyadenylate-binding protein-interacting protein 1 isoform 2 [Homo sapiens]" 100.00 400 100.00 100.00 1.67e-04 REF NP_899152 "polyadenylate-binding protein-interacting protein 1 isoform 3 [Homo sapiens]" 100.00 367 100.00 100.00 9.98e-05 REF XP_003811055 "PREDICTED: polyadenylate-binding protein-interacting protein 1 isoform X1 [Pan paniscus]" 100.00 479 100.00 100.00 2.91e-04 REF XP_003811056 "PREDICTED: polyadenylate-binding protein-interacting protein 1 isoform X2 [Pan paniscus]" 100.00 400 100.00 100.00 1.47e-04 SP Q9H074 "RecName: Full=Polyadenylate-binding protein-interacting protein 1; Short=PABP-interacting protein 1; Short=PAIP-1; Short=Poly(A" 100.00 479 100.00 100.00 3.23e-04 stop_ save_ save_Paip1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'fragment of Paip1' _Abbreviation_common 'Paip1 (126-147)' _Molecular_mass 2426 _Mol_thiol_state 'not present' _Details . _Residue_count 22 _Mol_residue_sequence ; VLMSKLSVNAPEFYPSGYSS SY ; loop_ _Residue_seq_code _Residue_label 1 VAL 2 LEU 3 MET 4 SER 5 LYS 6 LEU 7 SER 8 VAL 9 ASN 10 ALA 11 PRO 12 GLU 13 PHE 14 TYR 15 PRO 16 SER 17 GLY 18 TYR 19 SER 20 SER 21 SER 22 TYR stop_ _Sequence_homology_query_date 2008-08-19 _Sequence_homology_query_revised_last_date 2008-08-19 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value SWISS-PROT Q9H074 'Polyadenylate-binding protein-interacting protein 1 (Poly(A)-binding protein-interacting protein 1) (PABP-interacting protein 1) (PAIP-1)' 100.00 479 100.00 100.00 1.26e-03 REF NP_899152 'poly(A) binding protein interacting protein 1 isoform 3 [Homo sapiens]' 100.00 367 100.00 100.00 5.47e-04 REF NP_877590 'poly(A) binding protein interacting protein 1 isoform 2 [Homo sapiens]' 100.00 400 100.00 100.00 7.96e-04 REF NP_006442 'poly(A) binding protein interacting protein 1 isoform 1 [Homo sapiens]' 100.00 479 100.00 100.00 1.26e-03 GenBank ABW03359 'poly(A) binding protein interacting protein 1 [synthetic construct]' 100.00 479 100.00 100.00 1.25e-03 GenBank ABM82036 'poly(A) binding protein interacting protein 1 [synthetic construct]' 100.00 479 100.00 100.00 1.25e-03 GenBank AAH15937 'Poly(A) binding protein interacting protein 1 [Homo sapiens]' 100.00 479 100.00 100.00 1.25e-03 GenBank AAH05295 'Poly(A) binding protein interacting protein 1 [Homo sapiens]' 100.00 400 100.00 100.00 7.96e-04 GenBank AAC39697 'polyadenylate binding protein-interacting protein-1 [Homo sapiens]' 100.00 479 100.00 100.00 1.26e-03 EMBL CAB66854 'hypothetical protein [Homo sapiens]' 100.00 479 100.00 100.00 1.26e-03 PDB 1JH4 'Solution Structure Of The C-Terminal Pabc Domain Of Human Poly(A)-Binding Protein In Complex With The Peptide From Paip1' 95.45 22 100.00 100.00 1.28e-02 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $PABC human 9606 Eukaryota Metazoa Homo sapiens $Paip1 human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $PABC 'recombinant technology' . . . . . $Paip1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $PABC 3 mM [U-15N] $Paip1 3 mM [U-15N] 'phosphate buffer' 50 mM . NaCl 0.1 M . NaN3 1 mM . H2O 90 % . D2O 10 % . stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $PABC 3 mM . $Paip1 3 mM . 'phosphate buffer' 50 mM . NaCl 0.1 M . NaN3 1 mM . H2O 90 % . D2O 10 % . stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $PABC 3 mM . $Paip1 3 mM . 'phosphate buffer' 50 mM . NaCl 0.1 M . NaN3 1 mM . D2O 100 % . stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $PABC 3 mM . $Paip1 3 mM [U-15N] 'phosphate buffer' 50 mM . NaCl 0.1 M . NaN3 1 mM . H2O 90 % . D2O 10 % . stop_ save_ ############################ # Computer software used # ############################ save_XWINNMR _Saveframe_category software _Name XWINNMR _Version 2.1 loop_ _Task collection stop_ _Details 'Bruker Spectrospin' save_ save_GIFA _Saveframe_category software _Name GIFA _Version 4.31 loop_ _Task processing stop_ _Details Delsuc save_ save_XEASY _Saveframe_category software _Name XEASY _Version 1.3.13 loop_ _Task 'data analysis' stop_ _Details Wuthrich save_ save_ARIA _Saveframe_category software _Name ARIA _Version 0.9 loop_ _Task 'structure solution' stop_ _Details Nilges save_ save_CNS _Saveframe_category software _Name CNS _Version 0.9 loop_ _Task refinement stop_ _Details Brunger save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_15N-separated_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 15N-separated NOESY' _Sample_label . save_ save_2D_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label . save_ save_HNHA_3 _Saveframe_category NMR_applied_experiment _Experiment_name HNHA _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 15N-separated NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name HNHA _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.3 0.1 n/a temperature 303 1 K 'ionic strength' 0.1 . M pressure 1 . atm stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.0 . direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'polyadenylate-binding protein 1 (544-636)' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 3 LEU N N 121.4 0.25 1 2 . 3 LEU H H 8.54 0.01 1 3 . 4 GLY N N 108.6 0.25 1 4 . 4 GLY H H 8.40 0.01 1 5 . 5 SER N N 116.8 0.25 1 6 . 5 SER H H 8.22 0.01 1 7 . 7 LEU N N 121.9 0.25 1 8 . 7 LEU H H 8.38 0.01 1 9 . 7 LEU HA H 4.59 0.01 1 10 . 7 LEU HB2 H 1.55 0.01 2 11 . 7 LEU HB3 H 1.37 0.01 2 12 . 7 LEU HD1 H 0.91 0.01 2 13 . 7 LEU HD2 H 0.82 0.01 2 14 . 8 THR N N 113.2 0.25 1 15 . 8 THR H H 7.39 0.01 1 16 . 8 THR HA H 4.67 0.01 1 17 . 8 THR HG2 H 1.21 0.01 1 18 . 9 ALA N N 122.9 0.25 1 19 . 9 ALA H H 9.05 0.01 1 20 . 9 ALA HA H 3.99 0.01 1 21 . 9 ALA HB H 1.45 0.01 1 22 . 10 SER N N 112.7 0.25 1 23 . 10 SER H H 8.38 0.01 1 24 . 10 SER HA H 3.87 0.01 1 25 . 10 SER HB2 H 4.21 0.01 2 26 . 11 MET N N 121.4 0.25 1 27 . 11 MET H H 7.64 0.01 1 28 . 11 MET HA H 4.12 0.01 1 29 . 11 MET HB2 H 2.20 0.01 2 30 . 11 MET HB3 H 2.14 0.01 2 31 . 11 MET HG2 H 2.60 0.01 2 32 . 11 MET HG3 H 2.54 0.01 2 33 . 12 LEU N N 118.3 0.25 1 34 . 12 LEU H H 7.75 0.01 1 35 . 12 LEU HB2 H 1.70 0.01 2 36 . 12 LEU HG H 1.53 0.01 1 37 . 12 LEU HD1 H 0.93 0.01 2 38 . 12 LEU HD2 H 0.82 0.01 2 39 . 13 ALA N N 116.3 0.25 1 40 . 13 ALA H H 7.78 0.01 1 41 . 13 ALA HA H 4.07 0.01 1 42 . 13 ALA HB H 1.48 0.01 1 43 . 14 SER N N 110.0 0.25 1 44 . 14 SER H H 7.42 0.01 1 45 . 14 SER HA H 4.41 0.01 1 46 . 14 SER HB2 H 3.95 0.01 2 47 . 15 ALA N N 126.0 0.25 1 48 . 15 ALA H H 7.63 0.01 1 49 . 15 ALA HA H 4.85 0.01 1 50 . 15 ALA HB H 1.41 0.01 1 51 . 18 GLN N N 115.8 0.25 1 52 . 18 GLN H H 9.19 0.01 1 53 . 18 GLN HA H 4.20 0.01 1 54 . 18 GLN HB2 H 2.13 0.01 2 55 . 18 GLN HB3 H 2.03 0.01 2 56 . 18 GLN HG2 H 2.53 0.01 2 57 . 18 GLN HG3 H 2.43 0.01 2 58 . 19 GLU N N 119.9 0.25 1 59 . 19 GLU H H 7.61 0.01 1 60 . 19 GLU HA H 4.27 0.01 1 61 . 19 GLU HB2 H 2.19 0.01 2 62 . 19 GLU HB3 H 2.05 0.01 2 63 . 19 GLU HG2 H 2.33 0.01 2 64 . 20 GLN N N 118.3 0.25 1 65 . 20 GLN H H 8.05 0.01 1 66 . 20 GLN HA H 3.94 0.01 1 67 . 20 GLN HB2 H 2.50 0.01 2 68 . 20 GLN HB3 H 2.32 0.01 2 69 . 20 GLN HG2 H 2.61 0.01 2 70 . 20 GLN HG3 H 2.50 0.01 2 71 . 20 GLN HE21 H 6.70 0.01 2 72 . 20 GLN HE22 H 7.57 0.01 2 73 . 21 LYS N N 113.2 0.25 1 74 . 21 LYS H H 7.57 0.01 1 75 . 21 LYS HA H 4.06 0.01 1 76 . 21 LYS HB2 H 2.23 0.01 2 77 . 21 LYS HB3 H 1.68 0.01 2 78 . 21 LYS HG2 H 1.85 0.01 2 79 . 22 GLN N N 118.3 0.25 1 80 . 22 GLN H H 7.64 0.01 1 81 . 22 GLN HA H 3.96 0.01 1 82 . 22 GLN HB2 H 2.33 0.01 2 83 . 22 GLN HG2 H 2.56 0.01 2 84 . 23 MET N N 116.8 0.25 1 85 . 23 MET H H 8.39 0.01 1 86 . 23 MET HA H 4.06 0.01 1 87 . 23 MET HB2 H 2.22 0.01 2 88 . 23 MET HB3 H 2.02 0.01 2 89 . 23 MET HG2 H 2.79 0.01 2 90 . 23 MET HG3 H 2.60 0.01 2 91 . 24 LEU N N 118.3 0.25 1 92 . 24 LEU H H 7.75 0.01 1 93 . 24 LEU HA H 3.79 0.01 1 94 . 24 LEU HB2 H 1.96 0.01 2 95 . 24 LEU HB3 H 1.10 0.01 2 96 . 24 LEU HD1 H 0.77 0.01 2 97 . 24 LEU HD2 H 0.72 0.01 2 98 . 25 GLY N N 108.6 0.25 1 99 . 25 GLY H H 9.03 0.01 1 100 . 25 GLY HA2 H 2.96 0.01 2 101 . 25 GLY HA3 H 2.60 0.01 2 102 . 26 GLU N N 118.3 0.25 1 103 . 26 GLU H H 8.52 0.01 1 104 . 26 GLU HB2 H 2.19 0.01 2 105 . 26 GLU HB3 H 1.91 0.01 2 106 . 27 ARG N N 114.7 0.25 1 107 . 27 ARG H H 7.18 0.01 1 108 . 27 ARG HA H 4.18 0.01 1 109 . 27 ARG HB2 H 2.05 0.01 2 110 . 27 ARG HB3 H 1.88 0.01 2 111 . 27 ARG HD2 H 3.23 0.01 2 112 . 27 ARG HD3 H 3.19 0.01 2 113 . 27 ARG HE H 7.48 0.01 1 114 . 28 LEU N N 118.3 0.25 1 115 . 28 LEU H H 8.44 0.01 1 116 . 28 LEU HA H 3.85 0.01 1 117 . 28 LEU HB2 H 1.68 0.01 2 118 . 28 LEU HD1 H 0.82 0.01 2 119 . 29 PHE N N 116.8 0.25 1 120 . 29 PHE H H 8.45 0.01 1 121 . 29 PHE HA H 4.33 0.01 1 122 . 29 PHE HB2 H 3.30 0.01 2 123 . 29 PHE HB3 H 3.18 0.01 2 124 . 29 PHE HD1 H 7.12 0.01 3 125 . 29 PHE HE1 H 7.26 0.01 3 126 . 30 PRO HA H 4.21 0.01 1 127 . 31 LEU N N 115.8 0.25 1 128 . 31 LEU H H 6.81 0.01 1 129 . 31 LEU HA H 4.08 0.01 1 130 . 31 LEU HB2 H 2.04 0.01 2 131 . 31 LEU HB3 H 1.21 0.01 2 132 . 31 LEU HG H 1.77 0.01 1 133 . 31 LEU HD1 H 0.97 0.01 2 134 . 31 LEU HD2 H 0.88 0.01 2 135 . 32 ILE N N 119.4 0.25 1 136 . 32 ILE H H 7.89 0.01 1 137 . 32 ILE HA H 3.65 0.01 1 138 . 32 ILE HB H 1.70 0.01 1 139 . 32 ILE HG2 H 0.89 0.01 1 140 . 32 ILE HD1 H 0.82 0.01 1 141 . 33 GLN N N 122.4 0.25 1 142 . 33 GLN H H 8.94 0.01 1 143 . 33 GLN HA H 3.49 0.01 1 144 . 33 GLN HB2 H 1.75 0.01 2 145 . 33 GLN HG2 H 2.10 0.01 2 146 . 34 ALA N N 115.2 0.25 1 147 . 34 ALA H H 6.81 0.01 1 148 . 34 ALA HA H 4.08 0.01 1 149 . 34 ALA HB H 1.43 0.01 1 150 . 35 MET N N 114.2 0.25 1 151 . 35 MET H H 7.20 0.01 1 152 . 35 MET HA H 4.18 0.01 1 153 . 35 MET HB2 H 2.10 0.01 2 154 . 35 MET HB3 H 1.86 0.01 2 155 . 35 MET HG2 H 2.76 0.01 2 156 . 35 MET HG3 H 2.50 0.01 2 157 . 36 HIS N N 114.7 0.25 1 158 . 36 HIS H H 8.12 0.01 1 159 . 36 HIS HA H 4.87 0.01 1 160 . 36 HIS HB2 H 2.95 0.01 2 161 . 36 HIS HB3 H 2.50 0.01 2 162 . 36 HIS HD2 H 6.63 0.01 1 163 . 36 HIS HE1 H 7.96 0.01 1 164 . 38 THR N N 108.0 0.25 1 165 . 38 THR H H 8.27 0.01 1 166 . 38 THR HA H 4.43 0.01 1 167 . 38 THR HG2 H 1.32 0.01 1 168 . 39 LEU H H 7.57 0.01 1 169 . 39 LEU HA H 4.79 0.01 1 170 . 39 LEU HB2 H 1.64 0.01 2 171 . 39 LEU HB3 H 1.41 0.01 2 172 . 39 LEU HD1 H 0.90 0.01 2 173 . 40 ALA N N 121.4 0.25 1 174 . 40 ALA H H 7.42 0.01 1 175 . 40 ALA HA H 3.81 0.01 1 176 . 40 ALA HB H 1.20 0.01 1 177 . 41 GLY N N 109.1 0.25 1 178 . 41 GLY H H 9.30 0.01 1 179 . 41 GLY HA2 H 4.39 0.01 2 180 . 42 LYS N N 125.5 0.25 1 181 . 42 LYS H H 8.52 0.01 1 182 . 42 LYS HA H 4.07 0.01 1 183 . 42 LYS HB2 H 2.04 0.01 2 184 . 42 LYS HB3 H 1.83 0.01 2 185 . 42 LYS HG2 H 1.74 0.01 2 186 . 42 LYS HG3 H 1.49 0.01 2 187 . 43 ILE N N 117.3 0.25 1 188 . 43 ILE H H 8.67 0.01 1 189 . 43 ILE HA H 3.67 0.01 1 190 . 43 ILE HB H 1.67 0.01 1 191 . 43 ILE HG2 H 0.89 0.01 1 192 . 43 ILE HD1 H 0.57 0.01 1 193 . 44 THR N N 114.7 0.25 1 194 . 44 THR H H 8.44 0.01 1 195 . 44 THR HA H 4.64 0.01 1 196 . 44 THR HB H 3.58 0.01 1 197 . 44 THR HG2 H 0.81 0.01 1 198 . 45 GLY N N 106.0 0.25 1 199 . 45 GLY H H 7.86 0.01 1 200 . 45 GLY HA2 H 4.53 0.01 2 201 . 45 GLY HA3 H 3.69 0.01 2 202 . 46 MET H H 8.17 0.01 1 203 . 46 MET HA H 3.97 0.01 1 204 . 46 MET HB2 H 2.27 0.01 2 205 . 46 MET HB3 H 2.00 0.01 2 206 . 46 MET HG2 H 2.71 0.01 2 207 . 46 MET HG3 H 2.52 0.01 2 208 . 47 LEU N N 118.8 0.25 1 209 . 47 LEU H H 8.32 0.01 1 210 . 47 LEU HA H 3.93 0.01 1 211 . 47 LEU HB2 H 1.76 0.01 2 212 . 47 LEU HB3 H 1.53 0.01 2 213 . 47 LEU HG H 1.90 0.01 1 214 . 47 LEU HD1 H 0.82 0.01 2 215 . 47 LEU HD2 H 0.76 0.01 2 216 . 48 LEU N N 114.8 0.25 1 217 . 48 LEU H H 7.94 0.01 1 218 . 48 LEU HA H 4.12 0.01 1 219 . 48 LEU HB2 H 1.85 0.01 2 220 . 48 LEU HD1 H 0.60 0.01 2 221 . 48 LEU HD2 H 0.46 0.01 2 222 . 49 GLU N N 114.7 0.25 1 223 . 49 GLU H H 7.19 0.01 1 224 . 49 GLU HA H 4.23 0.01 1 225 . 49 GLU HB2 H 2.23 0.01 2 226 . 49 GLU HB3 H 2.04 0.01 2 227 . 49 GLU HG2 H 2.63 0.01 2 228 . 50 ILE N N 119.4 0.25 1 229 . 50 ILE H H 7.81 0.01 1 230 . 50 ILE HA H 3.97 0.01 1 231 . 50 ILE HB H 2.14 0.01 1 232 . 50 ILE HG2 H 1.00 0.01 1 233 . 50 ILE HG12 H 1.63 0.01 2 234 . 50 ILE HG13 H 1.51 0.01 2 235 . 50 ILE HD1 H 0.83 0.01 1 236 . 51 ASP N N 117.8 0.25 1 237 . 51 ASP H H 7.80 0.01 1 238 . 51 ASP HA H 4.27 0.01 1 239 . 51 ASP HB2 H 2.75 0.01 2 240 . 51 ASP HB3 H 2.65 0.01 2 241 . 52 ASN N N 122.4 0.25 1 242 . 52 ASN H H 8.69 0.01 1 243 . 52 ASN HA H 4.29 0.01 1 244 . 52 ASN HB2 H 2.90 0.01 2 245 . 52 ASN HB3 H 2.55 0.01 2 246 . 52 ASN HD21 H 7.32 0.01 2 247 . 52 ASN HD22 H 6.51 0.01 2 248 . 53 SER N N 114.2 0.25 1 249 . 53 SER H H 8.63 0.01 1 250 . 53 SER HA H 4.12 0.01 1 251 . 53 SER HB2 H 3.93 0.01 2 252 . 54 GLU N N 123.5 0.25 1 253 . 54 GLU H H 7.41 0.01 1 254 . 54 GLU HA H 4.29 0.01 1 255 . 54 GLU HB2 H 2.18 0.01 2 256 . 54 GLU HB3 H 2.10 0.01 2 257 . 54 GLU HG2 H 2.35 0.01 2 258 . 54 GLU HG3 H 2.30 0.01 2 259 . 55 LEU N N 120.3 0.25 1 260 . 55 LEU H H 7.81 0.01 1 261 . 55 LEU HA H 4.16 0.01 1 262 . 55 LEU HB2 H 2.14 0.01 2 263 . 55 LEU HB3 H 1.11 0.01 2 264 . 55 LEU HG H 1.69 0.01 1 265 . 55 LEU HD1 H 0.90 0.01 2 266 . 55 LEU HD2 H 0.74 0.01 2 267 . 56 LEU N N 117.3 0.25 1 268 . 56 LEU H H 8.39 0.01 1 269 . 56 LEU HA H 3.93 0.01 1 270 . 56 LEU HB2 H 1.75 0.01 2 271 . 56 LEU HD1 H 0.77 0.01 2 272 . 57 HIS N N 115.8 0.25 1 273 . 57 HIS H H 7.45 0.01 1 274 . 57 HIS HA H 4.54 0.01 1 275 . 57 HIS HB2 H 3.51 0.01 2 276 . 57 HIS HB3 H 3.34 0.01 2 277 . 57 HIS HD2 H 7.35 0.01 1 278 . 58 MET N N 118.4 0.25 1 279 . 58 MET H H 8.28 0.01 1 280 . 58 MET HA H 3.85 0.01 1 281 . 58 MET HB2 H 2.40 0.01 2 282 . 58 MET HG2 H 2.74 0.01 2 283 . 59 LEU N N 115.2 0.25 1 284 . 59 LEU H H 7.57 0.01 1 285 . 59 LEU HA H 3.94 0.01 1 286 . 59 LEU HB2 H 1.99 0.01 2 287 . 59 LEU HB3 H 1.54 0.01 2 288 . 59 LEU HD1 H 0.92 0.01 2 289 . 59 LEU HD2 H 0.77 0.01 2 290 . 60 GLU N N 115.7 0.25 1 291 . 60 GLU H H 7.28 0.01 1 292 . 60 GLU HA H 4.43 0.01 1 293 . 60 GLU HB2 H 2.12 0.01 2 294 . 60 GLU HG2 H 2.37 0.01 2 295 . 61 SER N N 113.2 0.25 1 296 . 61 SER H H 7.35 0.01 1 297 . 61 SER HA H 5.05 0.01 1 298 . 61 SER HB2 H 3.57 0.01 2 299 . 61 SER HB3 H 3.45 0.01 2 300 . 63 GLU N N 116.8 0.25 1 301 . 63 GLU H H 9.01 0.01 1 302 . 63 GLU HA H 4.11 0.01 1 303 . 63 GLU HB2 H 2.02 0.01 2 304 . 63 GLU HB3 H 1.96 0.01 2 305 . 63 GLU HG2 H 2.43 0.01 2 306 . 63 GLU HG3 H 2.35 0.01 2 307 . 64 SER N N 116.8 0.25 1 308 . 64 SER H H 7.82 0.01 1 309 . 64 SER HA H 4.37 0.01 1 310 . 64 SER HB2 H 3.96 0.01 2 311 . 65 LEU N N 122.4 0.25 1 312 . 65 LEU H H 7.92 0.01 1 313 . 65 LEU HA H 4.15 0.01 1 314 . 65 LEU HB2 H 1.94 0.01 2 315 . 65 LEU HB3 H 1.78 0.01 2 316 . 65 LEU HD1 H 0.76 0.01 2 317 . 66 ARG N N 117.2 0.25 1 318 . 66 ARG H H 8.38 0.01 1 319 . 66 ARG HA H 3.79 0.01 1 320 . 66 ARG HB2 H 1.96 0.01 2 321 . 66 ARG HG2 H 1.73 0.01 2 322 . 66 ARG HD2 H 3.27 0.01 2 323 . 67 SER N N 111.1 0.25 1 324 . 67 SER H H 7.75 0.01 1 325 . 67 SER HA H 4.31 0.01 1 326 . 67 SER HB2 H 4.04 0.01 2 327 . 68 LYS N N 121.9 0.25 1 328 . 68 LYS H H 7.59 0.01 1 329 . 68 LYS HA H 4.26 0.01 1 330 . 68 LYS HB2 H 2.07 0.01 2 331 . 68 LYS HB3 H 1.86 0.01 2 332 . 68 LYS HG2 H 1.60 0.01 2 333 . 69 VAL N N 120.9 0.25 1 334 . 69 VAL H H 8.75 0.01 1 335 . 69 VAL HA H 3.51 0.01 1 336 . 69 VAL HB H 2.30 0.01 1 337 . 69 VAL HG1 H 1.11 0.01 2 338 . 69 VAL HG2 H 1.02 0.01 2 339 . 70 ASP N N 118.8 0.25 1 340 . 70 ASP H H 8.66 0.01 1 341 . 70 ASP HA H 4.37 0.01 1 342 . 70 ASP HB2 H 2.87 0.01 2 343 . 70 ASP HB3 H 2.64 0.01 2 344 . 71 GLU N N 121.9 0.25 1 345 . 71 GLU H H 7.67 0.01 1 346 . 71 GLU HA H 4.08 0.01 1 347 . 71 GLU HB2 H 2.21 0.01 2 348 . 71 GLU HG2 H 2.48 0.01 2 349 . 71 GLU HG3 H 2.11 0.01 2 350 . 72 ALA N N 121.9 0.25 1 351 . 72 ALA H H 8.25 0.01 1 352 . 72 ALA HA H 3.84 0.01 1 353 . 72 ALA HB H 1.58 0.01 1 354 . 73 VAL N N 117.3 0.25 1 355 . 73 VAL H H 8.72 0.01 1 356 . 73 VAL HA H 3.30 0.01 1 357 . 73 VAL HB H 2.02 0.01 1 358 . 73 VAL HG1 H 0.72 0.01 2 359 . 73 VAL HG2 H 0.54 0.01 2 360 . 74 ALA N N 122.1 0.25 1 361 . 74 ALA H H 7.57 0.01 1 362 . 74 ALA HA H 4.15 0.01 1 363 . 74 ALA HB H 1.53 0.01 1 364 . 75 VAL N N 120.9 0.25 1 365 . 75 VAL H H 8.33 0.01 1 366 . 75 VAL HA H 3.73 0.01 1 367 . 75 VAL HB H 2.23 0.01 1 368 . 75 VAL HG1 H 1.10 0.01 2 369 . 75 VAL HG2 H 0.89 0.01 2 370 . 76 LEU N N 121.9 0.25 1 371 . 76 LEU H H 7.97 0.01 1 372 . 76 LEU HA H 3.90 0.01 1 373 . 76 LEU HB2 H 2.11 0.01 2 374 . 76 LEU HB3 H 1.63 0.01 2 375 . 76 LEU HD1 H 0.89 0.01 2 376 . 77 GLN N N 118.3 0.25 1 377 . 77 GLN H H 8.62 0.01 1 378 . 77 GLN HA H 4.13 0.01 1 379 . 77 GLN HB2 H 2.16 0.01 2 380 . 77 GLN HB3 H 2.01 0.01 2 381 . 77 GLN HG2 H 2.63 0.01 2 382 . 77 GLN HG3 H 2.36 0.01 2 383 . 78 ALA N N 121.9 0.25 1 384 . 78 ALA H H 7.81 0.01 1 385 . 78 ALA HA H 4.26 0.01 1 386 . 78 ALA HB H 1.52 0.01 1 387 . 79 HIS N N 118.4 0.25 1 388 . 79 HIS H H 8.17 0.01 1 389 . 79 HIS HA H 4.50 0.01 1 390 . 79 HIS HB2 H 3.34 0.01 2 391 . 79 HIS HB3 H 3.24 0.01 2 392 . 79 HIS HD2 H 7.18 0.01 1 393 . 81 ALA H H 8.01 0.01 1 394 . 81 ALA HA H 4.19 0.01 1 395 . 81 ALA HB H 1.48 0.01 1 396 . 82 LYS N N 119.6 0.25 1 397 . 82 LYS H H 7.91 0.01 1 398 . 82 LYS HA H 4.16 0.01 1 399 . 82 LYS HB2 H 1.88 0.01 2 400 . 82 LYS HG2 H 2.22 0.01 2 401 . 84 ALA N N 122.0 0.25 1 402 . 84 ALA H H 8.14 0.01 1 403 . 85 ALA N N 120.4 0.25 1 404 . 85 ALA H H 7.98 0.01 1 405 . 86 GLN N N 117.5 0.25 1 406 . 86 GLN H H 8.04 0.01 1 407 . 87 LYS N N 120.4 0.25 1 408 . 87 LYS H H 8.09 0.01 1 409 . 88 ALA N N 123.5 0.25 1 410 . 88 ALA H H 8.10 0.01 1 411 . 89 VAL N N 118.0 0.25 1 412 . 89 VAL H H 8.03 0.01 1 413 . 90 ASN N N 121.3 0.25 1 414 . 90 ASN H H 8.45 0.01 1 415 . 91 SER N N 116.0 0.25 1 416 . 91 SER H H 8.32 0.01 1 417 . 92 ALA N N 125.4 0.25 1 418 . 92 ALA H H 8.40 0.01 1 419 . 93 THR N N 111.1 0.25 1 420 . 93 THR H H 8.10 0.01 1 421 . 94 GLY N N 110.6 0.25 1 422 . 94 GLY H H 8.34 0.01 1 423 . 95 VAL N N 119.9 0.25 1 424 . 95 VAL H H 7.98 0.01 1 425 . 97 THR N N 115.2 0.25 1 426 . 97 THR H H 8.37 0.01 1 427 . 98 VAL N N 125.6 0.25 1 428 . 98 VAL H H 7.78 0.01 1 stop_ save_ save_chemical_shift_set_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'polyadenylate-binding protein-interacting protein-1 (126-147)' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 4 SER N N 114.8 0.25 1 2 . 4 SER H H 8.37 0.01 1 3 . 5 LYS N N 124.0 0.25 1 4 . 5 LYS H H 8.80 0.01 1 5 . 5 LYS HA H 4.39 0.01 1 6 . 5 LYS HB2 H 1.67 0.01 2 7 . 5 LYS HG2 H 1.94 0.01 2 8 . 5 LYS HG3 H 1.47 0.01 2 9 . 6 LEU N N 120.9 0.25 1 10 . 6 LEU H H 8.44 0.01 1 11 . 6 LEU HA H 4.45 0.01 1 12 . 6 LEU HB2 H 1.74 0.01 2 13 . 6 LEU HD1 H 0.77 0.01 2 14 . 7 SER N N 114.7 0.25 1 15 . 7 SER H H 9.03 0.01 1 16 . 7 SER HB2 H 3.91 0.01 2 17 . 8 VAL N N 121.4 0.25 1 18 . 8 VAL H H 8.52 0.01 1 19 . 8 VAL HA H 3.93 0.01 1 20 . 8 VAL HB H 2.04 0.01 1 21 . 8 VAL HG1 H 0.70 0.01 2 22 . 8 VAL HG2 H 0.46 0.01 2 23 . 9 ASN N N 117.3 0.25 1 24 . 9 ASN H H 7.94 0.01 1 25 . 9 ASN HA H 4.69 0.01 1 26 . 9 ASN HB2 H 2.89 0.01 2 27 . 9 ASN HB3 H 2.44 0.01 2 28 . 10 ALA N N 125.0 0.25 1 29 . 10 ALA H H 7.12 0.01 1 30 . 10 ALA HA H 4.33 0.01 1 31 . 10 ALA HB H 1.26 0.01 1 32 . 12 GLU N N 118.3 0.25 1 33 . 12 GLU H H 8.44 0.01 1 34 . 12 GLU HA H 3.89 0.01 1 35 . 12 GLU HB2 H 1.87 0.01 2 36 . 13 PHE N N 122.4 0.25 1 37 . 13 PHE HA H 4.42 0.01 1 38 . 13 PHE HB2 H 2.87 0.01 2 39 . 13 PHE HB3 H 2.64 0.01 2 40 . 13 PHE HD1 H 6.89 0.01 3 41 . 13 PHE HE1 H 6.95 0.01 3 42 . 13 PHE HZ H 7.18 0.01 1 43 . 14 TYR N N 121.4 0.25 1 44 . 14 TYR H H 7.04 0.01 1 45 . 14 TYR HA H 4.07 0.01 1 46 . 14 TYR HB2 H 2.32 0.01 2 47 . 14 TYR HB3 H 2.16 0.01 2 48 . 14 TYR HD1 H 6.64 0.01 3 49 . 14 TYR HE1 H 6.77 0.01 3 50 . 15 PRO HA H 4.08 0.01 1 51 . 16 SER N N 117.8 0.25 1 52 . 16 SER H H 9.35 0.01 1 53 . 16 SER HA H 4.17 0.01 1 54 . 16 SER HB2 H 3.84 0.01 2 55 . 17 GLY N N 111.6 0.25 1 56 . 17 GLY H H 8.73 0.01 1 57 . 17 GLY HA2 H 3.98 0.01 2 58 . 17 GLY HA3 H 3.82 0.01 2 59 . 18 TYR N N 120.4 0.25 1 60 . 18 TYR H H 7.77 0.01 1 61 . 18 TYR HA H 4.36 0.01 1 62 . 18 TYR HB2 H 2.93 0.01 2 63 . 18 TYR HB3 H 2.83 0.01 2 64 . 18 TYR HD1 H 6.96 0.01 3 65 . 18 TYR HE1 H 6.80 0.01 3 66 . 19 SER N N 117.8 0.25 1 67 . 19 SER H H 7.97 0.01 1 68 . 19 SER HA H 4.26 0.01 1 69 . 19 SER HB2 H 3.72 0.01 2 70 . 19 SER HB3 H 3.66 0.01 2 71 . 20 SER N N 116.9 0.25 1 72 . 20 SER H H 8.10 0.01 1 73 . 21 SER N N 117.2 0.25 1 74 . 21 SER H H 8.19 0.01 1 75 . 22 TYR N N 125.8 0.25 1 76 . 22 TYR H H 7.64 0.01 1 77 . 22 TYR HB2 H 3.06 0.01 2 78 . 22 TYR HB3 H 2.88 0.01 2 79 . 22 TYR HD1 H 7.09 0.01 3 stop_ save_ ######################## # Coupling constants # ######################## save_J_values_set_1 _Saveframe_category coupling_constants _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Spectrometer_frequency_1H 500 _Mol_system_component_name 'polyadenylate-binding protein-interacting protein-1 (126-147)' _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 3JHNHA 2 LEU H 2 LEU HA 7.1 . . 0.5 2 3JHNHA 3 MET H 3 MET HA 7.5 . . 0.5 3 3JHNHA 4 SER H 4 SER HA 6.6 . . 0.5 4 3JHNHA 5 LYS H 5 LYS HA 8.3 . . 0.5 5 3JHNHA 6 LEU H 6 LEU HA 6.2 . . 0.5 6 3JHNHA 7 SER H 7 SER HA 8.4 . . 0.5 7 3JHNHA 9 ASN H 9 ASN HA 4.6 . . 0.5 8 3JHNHA 10 ALA H 10 ALA HA 2.8 . . 0.5 9 3JHNHA 12 GLU H 12 GLU HA 4.5 . . 0.5 10 3JHNHA 13 PHE H 13 PHE HA 9.8 . . 0.5 11 3JHNHA 14 TYR H 14 TYR HA 8.6 . . 0.5 12 3JHNHA 16 SER H 16 SER HA 4.7 . . 0.5 13 3JHNHA 17 GLY H 17 GLY HA 5.2 . . 0.5 14 3JHNHA 18 TYR H 18 TYR HA 5.4 . . 0.5 15 3JHNHA 19 SER H 19 SER HA 7.4 . . 0.5 16 3JHNHA 20 SER H 20 SER HA 7.8 . . 0.5 17 3JHNHA 21 SER H 21 SER HA 8.9 . . 0.5 18 3JHNHA 22 TYR H 22 TYR HA 8.5 . . 0.5 stop_ save_