data_516 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Secondary Structure of the Human Growth Hormone Releasing Factor (GRF 1-29) by Two-Dimensional 1H-NMR Spectroscopy ; _BMRB_accession_number 516 _BMRB_flat_file_name bmr516.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Theriault Y. . . 2 Boulanger Y. . . 3 Saunders J. K. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 58 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-11 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Theriault, Y., Boulanger, Y., Saunders, J.K., "Secondary Structure of the Human Growth Hormone Releasing Factor (GRF 1-29) by Two-Dimensional 1H-NMR Spectroscopy," Biopolymers 27, 1897-1904 (1988). ; _Citation_title ; Secondary Structure of the Human Growth Hormone Releasing Factor (GRF 1-29) by Two-Dimensional 1H-NMR Spectroscopy ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Theriault Y. . . 2 Boulanger Y. . . 3 Saunders J. K. . stop_ _Journal_abbreviation Biopolymers _Journal_volume 27 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1897 _Page_last 1904 _Year 1988 _Details . save_ ################################## # Molecular system description # ################################## save_system_growth_hormone_releasing_factor _Saveframe_category molecular_system _Mol_system_name 'growth hormone releasing factor' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'growth hormone releasing factor' $growth_hormone_releasing_factor stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_growth_hormone_releasing_factor _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'growth hormone releasing factor' _Name_variant 'residues 1-29' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 29 _Mol_residue_sequence ; YADAIFTNSYRKVLGQLSAR KLLQDIMSR ; loop_ _Residue_seq_code _Residue_label 1 TYR 2 ALA 3 ASP 4 ALA 5 ILE 6 PHE 7 THR 8 ASN 9 SER 10 TYR 11 ARG 12 LYS 13 VAL 14 LEU 15 GLY 16 GLN 17 LEU 18 SER 19 ALA 20 ARG 21 LYS 22 LEU 23 LEU 24 GLN 25 ASP 26 ILE 27 MET 28 SER 29 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value EMBL CAA24955 "unnamed protein product [Homo sapiens]" 100.00 96 100.00 100.00 1.56e-10 EMBL CAA24956 "unnamed protein product [Homo sapiens]" 100.00 44 100.00 100.00 8.00e-11 GB AAA52608 "growth hormone releasing factor, partial [Homo sapiens]" 100.00 108 100.00 100.00 1.40e-10 GB AAA52609 "growth hormone releasing factor, partial [Homo sapiens]" 100.00 107 100.00 100.00 1.42e-10 GB AAA72764 "synthetic human growth hormone releasing factor (hGRF) [synthetic construct]" 100.00 45 100.00 100.00 7.46e-11 GB AAA72788 "fusion protein, partial [synthetic construct]" 93.10 32 100.00 100.00 2.51e-09 GB AAH98109 "Growth hormone releasing hormone [Homo sapiens]" 100.00 108 100.00 100.00 1.40e-10 PRF 0812170A "somatotropin releasing peptide" 100.00 40 100.00 100.00 6.92e-11 PRF 0901193A somatoliberin 100.00 40 100.00 100.00 6.92e-11 PRF 0912534A "somatoliberin precursor" 100.00 103 100.00 100.00 1.43e-10 REF NP_001171660 "somatoliberin isoform 2 preproprotein [Homo sapiens]" 100.00 107 100.00 100.00 1.42e-10 REF NP_001182047 "somatoliberin precursor [Sus scrofa]" 100.00 106 100.00 100.00 1.52e-10 REF NP_001277041 "somatoliberin precursor [Canis lupus familiaris]" 100.00 106 100.00 100.00 1.57e-10 REF NP_066567 "somatoliberin isoform 1 preproprotein [Homo sapiens]" 100.00 108 100.00 100.00 1.40e-10 REF XP_001093107 "PREDICTED: somatoliberin [Macaca mulatta]" 100.00 107 100.00 100.00 1.36e-10 SP P01286 "RecName: Full=Somatoliberin; AltName: Full=Growth hormone-releasing factor; Short=GRF; AltName: Full=Growth hormone-releasing h" 100.00 108 100.00 100.00 1.40e-10 SP P01287 "RecName: Full=Somatoliberin; AltName: Full=Growth hormone-releasing factor; Short=GRF; AltName: Full=Growth hormone-releasing h" 100.00 44 100.00 100.00 8.00e-11 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $growth_hormone_releasing_factor human 9606 Eukaryota Metazoa Homo sapiens generic stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $growth_hormone_releasing_factor 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.2 . na temperature 293 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'growth hormone releasing factor' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 TYR HA H 4.24 . 1 2 . 2 ALA H H 8.45 . 1 3 . 2 ALA HA H 4.37 . 1 4 . 3 ASP H H 8.33 . 1 5 . 3 ASP HA H 4.66 . 1 6 . 4 ALA H H 8.29 . 1 7 . 4 ALA HA H 4.29 . 1 8 . 5 ILE H H 7.75 . 1 9 . 5 ILE HA H 3.95 . 1 10 . 6 PHE H H 7.9 . 1 11 . 6 PHE HA H 4.51 . 1 12 . 7 THR H H 8.14 . 1 13 . 7 THR HA H 4.1 . 1 14 . 8 ASN H H 8.29 . 1 15 . 8 ASN HA H 4.63 . 1 16 . 9 SER H H 8.08 . 1 17 . 9 SER HA H 4.32 . 1 18 . 10 TYR H H 8.2 . 1 19 . 10 TYR HA H 4.29 . 1 20 . 11 ARG H H 8.02 . 1 21 . 11 ARG HA H 3.91 . 1 22 . 12 LYS H H 7.79 . 1 23 . 12 LYS HA H 4.14 . 1 24 . 13 VAL H H 7.85 . 1 25 . 13 VAL HA H 3.73 . 1 26 . 14 LEU H H 8.35 . 1 27 . 14 LEU HA H 4.09 . 1 28 . 15 GLY H H 8.34 . 1 29 . 15 GLY HA2 H 3.92 . 1 30 . 15 GLY HA3 H 3.92 . 1 31 . 16 GLN H H 7.93 . 1 32 . 16 GLN HA H 4.21 . 1 33 . 17 LEU H H 8.61 . 1 34 . 17 LEU HA H 4.15 . 1 35 . 18 SER H H 8.37 . 1 36 . 18 SER HA H 4.2 . 1 37 . 19 ALA H H 7.88 . 1 38 . 19 ALA HA H 4.18 . 1 39 . 20 ARG H H 8.09 . 1 40 . 20 ARG HA H 4.1 . 1 41 . 21 LYS H H 7.96 . 1 42 . 21 LYS HA H 4.05 . 1 43 . 22 LEU H H 8.02 . 1 44 . 22 LEU HA H 4.23 . 1 45 . 23 LEU H H 8.28 . 1 46 . 23 LEU HA H 4.12 . 1 47 . 24 GLN H H 8.22 . 1 48 . 24 GLN HA H 4.02 . 1 49 . 25 ASP H H 8.37 . 1 50 . 25 ASP HA H 4.47 . 1 51 . 26 ILE H H 8.59 . 1 52 . 26 ILE HA H 3.76 . 1 53 . 27 MET H H 8.65 . 1 54 . 27 MET HA H 4.31 . 1 55 . 28 SER H H 8.02 . 1 56 . 28 SER HA H 4.42 . 1 57 . 29 ARG H H 7.65 . 1 58 . 29 ARG HA H 4.33 . 1 stop_ save_