data_5173 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR structure of human Epiregulin ; _BMRB_accession_number 5173 _BMRB_flat_file_name bmr5173.str _Entry_type original _Submission_date 2001-10-01 _Accession_date 2001-10-01 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Sato Katsuharu . . 2 Miura Kazunori . . 3 Tada Masahito . . 4 Aizawa Tomoyasu . . 5 Miyamoto Kaoru . . 6 Kawano Keiichi . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 248 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2003-12-08 original author . stop_ _Original_release_date 2003-12-08 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Solution structure of epiregulin and the effect of its C-terminal domain for receptor binding affinity ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 14572630 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Sato Katsuharu . . 2 Nakamura T. . . 3 Mizuguchi M. . . 4 Miura Kazunori . . 5 Tada Masahito . . 6 Aizawa Tomoyasu . . 7 Gomi T. . . 8 Miyamoto Kaoru . . 9 Kawano Keiichi . . stop_ _Journal_abbreviation 'FEBS Lett.' _Journal_volume 553 _Journal_issue 3 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 232 _Page_last 238 _Year 2003 _Details . loop_ _Keyword Epiregulin growth-factor EGF-family NMR structure stop_ save_ ################################## # Molecular system description # ################################## save_system_Epiregulin _Saveframe_category molecular_system _Mol_system_name 'Epiregulin monomer' _Abbreviation_common Epiregulin _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Epiregulin $Epiregulin stop_ _System_molecular_weight . _System_physical_state 'molten globule' _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'all disulfide bound' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Epiregulin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Epiregulin _Abbreviation_common EPR _Molecular_mass . _Mol_thiol_state 'all disulfide bound' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 46 _Mol_residue_sequence ; VSITKCSSDMNGYCLHGQCI YLVDMSQNYCRCEVGYTGVR CEHFFL ; loop_ _Residue_seq_code _Residue_label 1 VAL 2 SER 3 ILE 4 THR 5 LYS 6 CYS 7 SER 8 SER 9 ASP 10 MET 11 ASN 12 GLY 13 TYR 14 CYS 15 LEU 16 HIS 17 GLY 18 GLN 19 CYS 20 ILE 21 TYR 22 LEU 23 VAL 24 ASP 25 MET 26 SER 27 GLN 28 ASN 29 TYR 30 CYS 31 ARG 32 CYS 33 GLU 34 VAL 35 GLY 36 TYR 37 THR 38 GLY 39 VAL 40 ARG 41 CYS 42 GLU 43 HIS 44 PHE 45 PHE 46 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1K36 "Nmr Structure Of Human Epiregulin" 100.00 46 100.00 100.00 1.18e-24 PDB 1K37 "Nmr Structure Of Human Epiregulin" 100.00 46 100.00 100.00 1.18e-24 DBJ BAA22146 "epiregulin [Homo sapiens]" 100.00 169 100.00 100.00 5.50e-25 DBJ BAG37463 "unnamed protein product [Homo sapiens]" 100.00 169 100.00 100.00 5.50e-25 EMBL CAG46685 "EREG [Homo sapiens]" 100.00 169 100.00 100.00 5.50e-25 GB AAI36405 "Epiregulin [Homo sapiens]" 100.00 169 100.00 100.00 5.50e-25 GB AAI36406 "Epiregulin [Homo sapiens]" 100.00 169 100.00 100.00 5.50e-25 GB AAX36706 "epiregulin [synthetic construct]" 100.00 170 100.00 100.00 5.99e-25 GB AIC54356 "EREG, partial [synthetic construct]" 100.00 169 100.00 100.00 5.50e-25 GB EAX05709 "epiregulin [Homo sapiens]" 100.00 169 100.00 100.00 5.50e-25 REF NP_001423 "proepiregulin preproprotein [Homo sapiens]" 100.00 169 100.00 100.00 5.50e-25 REF XP_001155238 "PREDICTED: proepiregulin [Pan troglodytes]" 100.00 169 100.00 100.00 5.50e-25 REF XP_002814919 "PREDICTED: proepiregulin [Pongo abelii]" 100.00 169 100.00 100.00 5.38e-25 REF XP_002919193 "PREDICTED: proepiregulin-like [Ailuropoda melanoleuca]" 100.00 171 97.83 97.83 4.08e-24 REF XP_003265794 "PREDICTED: proepiregulin [Nomascus leucogenys]" 100.00 169 100.00 100.00 5.38e-25 SP O14944 "RecName: Full=Proepiregulin; Contains: RecName: Full=Epiregulin; Short=EPR; Flags: Precursor [Homo sapiens]" 100.00 169 100.00 100.00 5.50e-25 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Epiregulin Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Epiregulin 'recombinant technology' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Epiregulin 1.5 mM . stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 500 _Details . save_ ####################### # Sample conditions # ####################### save_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 3.4 0.1 n/a temperature 303 1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name Epiregulin _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 2 SER H H 8.81 0.03 1 2 . 2 SER HA H 4.65 0.03 1 3 . 2 SER HB2 H 3.81 0.03 1 4 . 2 SER HB3 H 3.81 0.03 1 5 . 3 ILE H H 8.19 0.03 1 6 . 3 ILE HA H 5.08 0.03 1 7 . 3 ILE HB H 1.78 0.03 1 8 . 3 ILE HG12 H 1.04 0.03 4 9 . 3 ILE HG13 H 1.04 0.03 4 10 . 3 ILE HG2 H 0.89 0.03 4 11 . 3 ILE HD1 H 0.81 0.03 4 12 . 4 THR H H 8.85 0.03 1 13 . 4 THR HA H 4.73 0.03 1 14 . 4 THR HB H 4.37 0.03 1 15 . 4 THR HG2 H 1.22 0.03 1 16 . 5 LYS H H 8.18 0.03 1 17 . 5 LYS HA H 4.50 0.03 1 18 . 5 LYS HB2 H 1.78 0.03 4 19 . 5 LYS HB3 H 1.89 0.03 4 20 . 5 LYS HG2 H 1.47 0.03 4 21 . 5 LYS HG3 H 1.47 0.03 4 22 . 5 LYS HD2 H 1.69 0.03 4 23 . 5 LYS HD3 H 1.69 0.03 4 24 . 5 LYS HE2 H 3.00 0.03 4 25 . 5 LYS HE3 H 3.00 0.03 4 26 . 6 CYS H H 8.61 0.03 1 27 . 6 CYS HA H 4.24 0.03 1 28 . 6 CYS HB2 H 3.03 0.03 2 29 . 6 CYS HB3 H 3.14 0.03 2 30 . 7 SER H H 8.54 0.03 1 31 . 7 SER HA H 4.28 0.03 1 32 . 7 SER HB2 H 3.72 0.03 2 33 . 7 SER HB3 H 3.94 0.03 2 34 . 8 SER H H 8.80 0.03 1 35 . 8 SER HA H 4.12 0.03 1 36 . 8 SER HB2 H 3.90 0.03 1 37 . 8 SER HB3 H 3.90 0.03 1 38 . 9 ASP H H 8.30 0.03 1 39 . 9 ASP HA H 4.47 0.03 1 40 . 9 ASP HB2 H 2.76 0.03 1 41 . 9 ASP HB3 H 2.76 0.03 1 42 . 10 MET H H 7.71 0.03 1 43 . 10 MET HA H 4.67 0.03 1 44 . 10 MET HB2 H 1.98 0.03 4 45 . 10 MET HB3 H 2.24 0.03 4 46 . 10 MET HG2 H 2.52 0.03 4 47 . 10 MET HG3 H 2.52 0.03 4 48 . 11 ASN H H 7.74 0.03 1 49 . 11 ASN HA H 4.43 0.03 1 50 . 11 ASN HB2 H 2.85 0.03 1 51 . 11 ASN HB3 H 2.85 0.03 1 52 . 12 GLY H H 8.63 0.03 1 53 . 12 GLY HA2 H 3.89 0.03 1 54 . 12 GLY HA3 H 3.89 0.03 1 55 . 13 TYR H H 7.88 0.03 1 56 . 13 TYR HA H 4.14 0.03 1 57 . 13 TYR HB2 H 2.80 0.03 2 58 . 13 TYR HB3 H 3.14 0.03 2 59 . 13 TYR HD1 H 6.96 0.03 1 60 . 13 TYR HD2 H 6.96 0.03 1 61 . 13 TYR HE1 H 6.64 0.03 1 62 . 13 TYR HE2 H 6.64 0.03 1 63 . 14 CYS H H 8.65 0.03 1 64 . 14 CYS HA H 4.25 0.03 1 65 . 14 CYS HB2 H 1.93 0.03 1 66 . 14 CYS HB3 H 2.54 0.03 2 67 . 15 LEU H H 8.31 0.03 2 68 . 15 LEU HA H 3.89 0.03 1 69 . 15 LEU HB2 H 1.35 0.03 1 70 . 15 LEU HB3 H 1.35 0.03 1 71 . 15 LEU HG H 1.00 0.03 4 72 . 15 LEU HD1 H 0.75 0.03 4 73 . 15 LEU HD2 H 0.75 0.03 4 74 . 16 HIS H H 6.08 0.03 1 75 . 16 HIS HA H 4.49 0.03 1 76 . 16 HIS HB2 H 1.99 0.03 2 77 . 16 HIS HB3 H 2.59 0.03 2 78 . 16 HIS HD2 H 8.46 0.03 1 79 . 16 HIS HE1 H 6.99 0.03 1 80 . 17 GLY H H 7.37 0.03 1 81 . 17 GLY HA2 H 4.04 0.03 2 82 . 17 GLY HA3 H 4.24 0.03 2 83 . 18 GLN H H 8.25 0.03 1 84 . 18 GLN HA H 4.58 0.03 1 85 . 18 GLN HB2 H 1.94 0.03 4 86 . 18 GLN HB3 H 2.01 0.03 4 87 . 18 GLN HG2 H 2.30 0.03 4 88 . 18 GLN HG3 H 2.30 0.03 4 89 . 19 CYS H H 8.94 0.03 1 90 . 19 CYS HA H 5.01 0.03 1 91 . 19 CYS HB2 H 3.14 0.03 1 92 . 19 CYS HB3 H 3.14 0.03 1 93 . 20 ILE H H 9.78 0.03 1 94 . 20 ILE HA H 4.58 0.03 1 95 . 20 ILE HB H 1.78 0.03 1 96 . 20 ILE HG12 H 1.27 0.03 4 97 . 20 ILE HG13 H 1.51 0.03 4 98 . 20 ILE HG2 H 0.92 0.03 4 99 . 20 ILE HD1 H 0.82 0.03 4 100 . 21 TYR H H 8.77 0.03 1 101 . 21 TYR HA H 5.06 0.03 1 102 . 21 TYR HB2 H 2.80 0.03 2 103 . 21 TYR HB3 H 2.49 0.03 2 104 . 21 TYR HD1 H 6.93 0.03 1 105 . 21 TYR HD2 H 6.93 0.03 1 106 . 21 TYR HE1 H 6.55 0.03 1 107 . 21 TYR HE2 H 6.55 0.03 1 108 . 22 LEU H H 8.37 0.03 1 109 . 22 LEU HA H 4.50 0.03 1 110 . 22 LEU HB2 H 1.56 0.03 2 111 . 22 LEU HB3 H 1.67 0.03 2 112 . 22 LEU HG H 1.40 0.03 4 113 . 22 LEU HD1 H 0.67 0.03 4 114 . 22 LEU HD2 H 0.67 0.03 4 115 . 23 VAL H H 7.78 0.03 1 116 . 23 VAL HA H 3.32 0.03 1 117 . 23 VAL HB H 1.89 0.03 1 118 . 23 VAL HG1 H 1.03 0.03 2 119 . 23 VAL HG2 H 0.94 0.03 2 120 . 24 ASP H H 8.92 0.03 1 121 . 24 ASP HA H 4.40 0.03 1 122 . 24 ASP HB2 H 2.68 0.03 2 123 . 24 ASP HB3 H 2.80 0.03 2 124 . 25 MET H H 7.02 0.03 1 125 . 25 MET HA H 4.55 0.03 1 126 . 25 MET HB2 H 1.62 0.03 4 127 . 25 MET HB3 H 2.17 0.03 4 128 . 25 MET HG2 H 2.39 0.03 4 129 . 25 MET HG3 H 2.46 0.03 4 130 . 26 SER H H 7.75 0.03 1 131 . 26 SER HA H 4.00 0.03 1 132 . 26 SER HB2 H 3.84 0.03 1 133 . 26 SER HB3 H 3.84 0.03 1 134 . 27 GLN H H 6.78 0.03 1 135 . 27 GLN HA H 4.68 0.03 1 136 . 27 GLN HB2 H 1.66 0.03 4 137 . 27 GLN HB3 H 1.66 0.03 4 138 . 27 GLN HG2 H 1.95 0.03 4 139 . 27 GLN HG3 H 2.24 0.03 4 140 . 28 ASN H H 8.55 0.03 1 141 . 28 ASN HA H 5.35 0.03 1 142 . 28 ASN HB2 H 2.45 0.03 1 143 . 28 ASN HB3 H 2.45 0.03 1 144 . 29 TYR H H 9.49 0.03 1 145 . 29 TYR HA H 4.84 0.03 1 146 . 29 TYR HB2 H 2.85 0.03 1 147 . 29 TYR HB3 H 2.85 0.03 1 148 . 29 TYR HD1 H 6.93 0.03 1 149 . 29 TYR HD2 H 6.93 0.03 1 150 . 29 TYR HE1 H 6.68 0.03 1 151 . 29 TYR HE2 H 6.68 0.03 1 152 . 30 CYS H H 8.45 0.03 1 153 . 30 CYS HA H 5.08 0.03 1 154 . 30 CYS HB2 H 2.69 0.03 2 155 . 30 CYS HB3 H 2.44 0.03 2 156 . 31 ARG H H 9.01 0.03 1 157 . 31 ARG HA H 4.50 0.03 1 158 . 31 ARG HB2 H 1.83 0.03 2 159 . 31 ARG HB3 H 2.01 0.03 2 160 . 31 ARG HG2 H 1.64 0.03 4 161 . 31 ARG HG3 H 1.70 0.03 4 162 . 31 ARG HD2 H 3.25 0.03 4 163 . 31 ARG HD3 H 3.25 0.03 4 164 . 32 CYS H H 9.02 0.03 1 165 . 32 CYS HA H 5.04 0.03 1 166 . 32 CYS HB2 H 2.75 0.03 2 167 . 32 CYS HB3 H 3.45 0.03 2 168 . 33 GLU H H 8.41 0.03 1 169 . 33 GLU HA H 4.35 0.03 1 170 . 33 GLU HB2 H 1.88 0.03 4 171 . 33 GLU HB3 H 2.22 0.03 4 172 . 33 GLU HG2 H 2.33 0.03 4 173 . 33 GLU HG3 H 2.57 0.03 4 174 . 34 VAL H H 8.33 0.03 1 175 . 34 VAL HA H 3.71 0.03 1 176 . 34 VAL HB H 1.96 0.03 1 177 . 34 VAL HG1 H 1.05 0.03 2 178 . 34 VAL HG2 H 0.86 0.03 2 179 . 35 GLY H H 8.66 0.03 1 180 . 35 GLY HA2 H 3.79 0.03 2 181 . 35 GLY HA3 H 4.58 0.03 2 182 . 36 TYR H H 8.05 0.03 1 183 . 36 TYR HA H 5.58 0.03 1 184 . 36 TYR HB2 H 2.99 0.03 2 185 . 36 TYR HB3 H 2.74 0.03 2 186 . 36 TYR HD1 H 6.68 0.03 1 187 . 36 TYR HD2 H 6.68 0.03 1 188 . 36 TYR HE1 H 6.27 0.03 1 189 . 36 TYR HE2 H 6.27 0.03 1 190 . 37 THR H H 9.26 0.03 1 191 . 37 THR HA H 4.94 0.03 1 192 . 37 THR HB H 4.14 0.03 1 193 . 37 THR HG2 H 1.11 0.03 1 194 . 38 GLY H H 8.10 0.03 1 195 . 38 GLY HA2 H 3.92 0.03 2 196 . 38 GLY HA3 H 5.10 0.03 2 197 . 39 VAL H H 9.13 0.03 1 198 . 39 VAL HA H 3.81 0.03 1 199 . 39 VAL HB H 2.11 0.03 1 200 . 39 VAL HG1 H 1.11 0.03 2 201 . 39 VAL HG2 H 1.05 0.03 2 202 . 40 ARG H H 8.26 0.03 1 203 . 40 ARG HA H 4.58 0.03 1 204 . 40 ARG HB2 H 1.13 0.03 2 205 . 40 ARG HB3 H 1.31 0.03 2 206 . 40 ARG HG2 H 0.44 0.03 4 207 . 40 ARG HG3 H 0.44 0.03 4 208 . 40 ARG HD2 H 2.83 0.03 4 209 . 40 ARG HD3 H 2.92 0.03 4 210 . 41 CYS H H 8.05 0.03 1 211 . 41 CYS HA H 3.86 0.03 1 212 . 41 CYS HB2 H 2.39 0.03 2 213 . 41 CYS HB3 H 2.92 0.03 2 214 . 42 GLU H H 9.67 0.03 1 215 . 42 GLU HA H 4.38 0.03 1 216 . 42 GLU HB2 H 1.80 0.03 4 217 . 42 GLU HB3 H 1.80 0.03 4 218 . 42 GLU HG2 H 2.04 0.03 4 219 . 42 GLU HG3 H 2.04 0.03 4 220 . 43 HIS H H 8.86 0.03 1 221 . 43 HIS HA H 5.10 0.03 1 222 . 43 HIS HB2 H 3.13 0.03 1 223 . 43 HIS HB3 H 3.13 0.03 1 224 . 43 HIS HD2 H 8.67 0.03 1 225 . 43 HIS HE1 H 7.13 0.03 1 226 . 44 PHE H H 8.83 0.03 1 227 . 44 PHE HA H 4.53 0.03 1 228 . 44 PHE HB2 H 2.68 0.03 2 229 . 44 PHE HB3 H 2.77 0.03 2 230 . 44 PHE HD1 H 6.35 0.03 1 231 . 44 PHE HD2 H 6.35 0.03 1 232 . 44 PHE HE1 H 7.04 0.03 3 233 . 44 PHE HE2 H 7.10 0.03 3 234 . 45 PHE H H 8.13 0.03 1 235 . 45 PHE HA H 4.42 0.03 1 236 . 45 PHE HB2 H 2.59 0.03 2 237 . 45 PHE HB3 H 3.00 0.03 2 238 . 45 PHE HD1 H 6.91 0.03 1 239 . 45 PHE HD2 H 6.91 0.03 1 240 . 45 PHE HE1 H 7.15 0.03 1 241 . 45 PHE HE2 H 7.15 0.03 1 242 . 46 LEU H H 6.19 0.03 1 243 . 46 LEU HA H 4.01 0.03 1 244 . 46 LEU HB2 H 1.09 0.03 2 245 . 46 LEU HB3 H 1.31 0.03 2 246 . 46 LEU HG H 0.88 0.03 4 247 . 46 LEU HD1 H 0.65 0.03 4 248 . 46 LEU HD2 H 0.65 0.03 4 stop_ loop_ _Atom_shift_assign_ID_ambiguity 11 '11,11,10,10,10,9,8' '25,24,23,22,21,20,19,18' '47,46,45,44' '73,73,73,72,72,72,71' '88,87,86,85' '99,99,99,98,98,98,97,96' '114,114,114,113,113,113,112' '129,128,127,126' '139,138,137,136' '163,162,161,160' '173,172,171,170' '209,208,207,206' '219,218,217,216' '248,248,248,247,247,247,246' stop_ save_