data_5464 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution Structure of a CCHC Zinc Finger from MOZ ; _BMRB_accession_number 5464 _BMRB_flat_file_name bmr5464.str _Entry_type original _Submission_date 2002-07-16 _Accession_date 2002-07-16 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kwan A. H.Y. . 2 Gell D. A. . 3 Liew C. K. . 4 Mackay J. P. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 coupling_constants 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 192 "coupling constants" 5 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2004-09-14 original author . stop_ _Original_release_date 2004-09-14 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Solution Structure of a CCHC Zinc Finger from MOZ' _Citation_status published _Citation_type 'BMRB only' _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kwan A. H.Y. . 2 Gell D. A. . 3 Liew C. K. . 4 Mackay J. P. . stop_ _Journal_abbreviation . _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . loop_ _Keyword 'zinc finger' 'acetyl transferase' stop_ save_ ################################## # Molecular system description # ################################## save_system_zinc_finger _Saveframe_category molecular_system _Mol_system_name 'Monocytic leukemia zinc finger protein' _Abbreviation_common MOZ _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Monocytic leukemia zinc finger protein' $MOZ 'Zinc ion' $ZN stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'all other bound' loop_ _Biological_function 'DNA binding protein' stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_MOZ _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Zinc finger protein' _Abbreviation_common MOZ _Molecular_mass . _Mol_thiol_state 'all other bound' _Details ; In the sample, a GLY residue and a SER residue were present at the amino end of the MOZ protein as a result of a thrombin cleavage used in the purification process and are not part of the MOZ protein. ; ############################## # Polymer residue sequence # ############################## _Residue_count 31 _Mol_residue_sequence ; RLPKLYLCEFCLKYMKSRTI LQQHMKKCGWF ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 533 ARG 2 534 LEU 3 535 PRO 4 536 LYS 5 537 LEU 6 538 TYR 7 539 LEU 8 540 CYS 9 541 GLU 10 542 PHE 11 543 CYS 12 544 LEU 13 545 LYS 14 546 TYR 15 547 MET 16 548 LYS 17 549 SER 18 550 ARG 19 551 THR 20 552 ILE 21 553 LEU 22 554 GLN 23 555 GLN 24 556 HIS 25 557 MET 26 558 LYS 27 559 LYS 28 560 CYS 29 561 GLY 30 562 TRP 31 563 PHE stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1M36 "Solution Structure Of A Cchc Zinc Finger From Moz" 100.00 33 100.00 100.00 3.91e-13 PDB 2OZU "Crystal Structure Of Human Myst Histone Acetyltransferase 3 In Complex With Acetylcoenzyme A" 100.00 284 100.00 100.00 1.66e-12 PDB 2RC4 "Crystal Structure Of The Hat Domain Of The Human Moz Protein" 100.00 287 100.00 100.00 1.65e-12 DBJ BAC25728 "unnamed protein product [Mus musculus]" 100.00 803 100.00 100.00 3.77e-12 DBJ BAC29621 "unnamed protein product [Mus musculus]" 100.00 1010 100.00 100.00 2.51e-12 DBJ BAD00088 "chimeric MOZ-ASXH2 fusion protein [Homo sapiens]" 100.00 2228 100.00 100.00 1.22e-14 DBJ BAD72833 "monocytic leukemia zinc finger protein [Rattus norvegicus]" 100.00 1991 100.00 100.00 1.19e-14 DBJ BAE21185 "unnamed protein product [Mus musculus]" 100.00 1148 100.00 100.00 1.88e-12 EMBL CAH89880 "hypothetical protein [Pongo abelii]" 100.00 1275 100.00 100.00 1.68e-13 GB AAC50662 "monocytic leukaemia zinc finger protein [Homo sapiens]" 100.00 2004 100.00 100.00 1.52e-14 GB AAH24786 "Myst3 protein, partial [Mus musculus]" 100.00 693 100.00 100.00 1.03e-12 GB AAI42660 "MYST3 protein, partial [Homo sapiens]" 100.00 1149 100.00 100.00 1.74e-12 GB AAI42960 "MYST3 protein [Homo sapiens]" 100.00 815 100.00 100.00 3.59e-12 GB AAI72379 "MYST histone acetyltransferase (monocytic leukemia) 3 [synthetic construct]" 100.00 2004 100.00 100.00 1.52e-14 REF NP_001074618 "histone acetyltransferase KAT6A [Mus musculus]" 100.00 2003 100.00 100.00 1.62e-14 REF NP_001092882 "histone acetyltransferase KAT6A [Homo sapiens]" 100.00 2004 100.00 100.00 1.52e-14 REF NP_001092883 "histone acetyltransferase KAT6A [Homo sapiens]" 100.00 2004 100.00 100.00 1.52e-14 REF NP_001094040 "histone acetyltransferase KAT6A [Rattus norvegicus]" 100.00 1998 100.00 100.00 1.19e-14 REF NP_006757 "histone acetyltransferase KAT6A [Homo sapiens]" 100.00 2004 100.00 100.00 1.52e-14 SP Q5TKR9 "RecName: Full=Histone acetyltransferase KAT6A; AltName: Full=MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3; Short=MYST-3; AltName: F" 100.00 1998 100.00 100.00 1.19e-14 SP Q8BZ21 "RecName: Full=Histone acetyltransferase KAT6A; AltName: Full=MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3; Short=MYST-3; AltName: F" 100.00 2003 100.00 100.00 1.64e-14 SP Q92794 "RecName: Full=Histone acetyltransferase KAT6A; AltName: Full=MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3; Short=MYST-3; AltName: F" 100.00 2004 100.00 100.00 1.52e-14 TPG DAA14459 "TPA: MYST histone acetyltransferase (monocytic leukemia) 3 [Bos taurus]" 100.00 2018 100.00 100.00 1.19e-14 stop_ save_ ############# # Ligands # ############# save_ZN _Saveframe_category ligand _Mol_type non-polymer _Name_common "ZN (ZINC ION)" _BMRB_code . _PDB_code ZN _Molecular_mass 65.409 _Mol_charge 2 _Mol_paramagnetic . _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Thu Jun 2 09:48:05 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons ZN ZN ZN . 2 . ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $MOZ Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $MOZ 'recombinant technology' 'E. coli' Escherichia coli BL21(DE3) plasmid PGEX-2T stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $MOZ 0.5 mM . $ZN 0.7 mM . TCEP 1 mM . H2O 95 % . D2O 5 % . stop_ save_ ############################ # Computer software used # ############################ save_XWINNMR _Saveframe_category software _Name XWINNMR _Version 2.5 loop_ _Task processing stop_ _Details Bruker save_ save_XEASY _Saveframe_category software _Name XEASY _Version 1.3.13 loop_ _Task 'data analysis' stop_ _Details 'Bartels et al' save_ save_DYANA _Saveframe_category software _Name DYANA _Version 1.5 loop_ _Task refinement stop_ _Details 'Guntert et al' save_ save_ARIA _Saveframe_category software _Name ARIA _Version 1.1.2 loop_ _Task 'structure solution' stop_ _Details 'Linge et al' save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_1 save_ save_2D_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _Sample_label $sample_1 save_ save_DQF-COSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _Sample_label $sample_1 save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.6 0.1 n/a temperature 298 0.2 K pressure 1 . atm stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label . H 1 . . . . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'Monocytic leukemia zinc finger protein' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 ARG H H 8.542 0.001 . 2 . 1 ARG HA H 4.373 0.002 . 3 . 1 ARG HB2 H 1.632 0.005 . 4 . 1 ARG HG2 H 1.750 0.003 . 5 . 1 ARG HG3 H 1.842 0.008 . 6 . 1 ARG HD2 H 3.194 0.004 . 7 . 1 ARG HE H 7.204 0.001 . 8 . 2 LEU H H 8.292 0.004 . 9 . 2 LEU HA H 4.591 0.004 . 10 . 2 LEU HB2 H 1.557 0.010 . 11 . 2 LEU HB3 H 1.651 0.003 . 12 . 2 LEU HG H 1.533 0.006 . 13 . 2 LEU HD2 H 0.901 0.003 . 14 . 3 PRO HA H 4.385 0.003 . 15 . 3 PRO HB2 H 1.800 0.009 . 16 . 3 PRO HB3 H 2.254 0.004 . 17 . 3 PRO HG2 H 1.982 0.007 . 18 . 3 PRO HD2 H 3.627 0.003 . 19 . 3 PRO HD3 H 3.825 0.003 . 20 . 4 LYS H H 8.261 0.001 . 21 . 4 LYS HA H 4.111 0.003 . 22 . 4 LYS HB2 H 1.599 0.003 . 23 . 4 LYS HG2 H 1.179 0.004 . 24 . 4 LYS HG3 H 1.260 0.007 . 25 . 4 LYS HD2 H 1.576 0.005 . 26 . 4 LYS HE2 H 2.887 0.002 . 27 . 5 LEU H H 7.626 0.003 . 28 . 5 LEU HA H 4.476 0.002 . 29 . 5 LEU HB2 H 1.233 0.003 . 30 . 5 LEU HB3 H 1.321 0.003 . 31 . 5 LEU HG H 1.389 0.009 . 32 . 5 LEU HD1 H 0.635 0.003 . 33 . 5 LEU HD2 H 0.711 0.004 . 34 . 6 TYR H H 8.559 0.003 . 35 . 6 TYR HA H 4.549 0.002 . 36 . 6 TYR HB2 H 2.713 0.006 . 37 . 6 TYR HB3 H 2.825 0.003 . 38 . 6 TYR HD1 H 6.952 0.007 . 39 . 6 TYR HE1 H 6.782 0.003 . 40 . 7 LEU H H 8.313 0.003 . 41 . 7 LEU HA H 4.023 0.003 . 42 . 7 LEU HB2 H 1.091 0.004 . 43 . 7 LEU HB3 H 1.561 0.005 . 44 . 7 LEU HG H 0.875 0.005 . 45 . 7 LEU HD1 H -0.136 0.004 . 46 . 7 LEU HD2 H 0.623 0.007 . 47 . 8 CYS H H 8.509 0.002 . 48 . 8 CYS HA H 4.539 0.005 . 49 . 8 CYS HB2 H 2.430 0.005 . 50 . 8 CYS HB3 H 3.306 0.005 . 51 . 9 GLU H H 8.404 0.004 . 52 . 9 GLU HA H 3.908 0.004 . 53 . 9 GLU HB2 H 1.679 0.005 . 54 . 9 GLU HB3 H 1.774 0.004 . 55 . 9 GLU HG2 H 1.842 0.007 . 56 . 9 GLU HG3 H 1.914 0.001 . 57 . 10 PHE H H 9.024 0.001 . 58 . 10 PHE HA H 4.549 0.004 . 59 . 10 PHE HB2 H 3.042 0.003 . 60 . 10 PHE HB3 H 3.484 0.004 . 61 . 10 PHE HD1 H 7.069 0.003 . 62 . 10 PHE HE1 H 7.208 0.005 . 63 . 11 CYS H H 8.196 0.002 . 64 . 11 CYS HA H 4.871 0.002 . 65 . 11 CYS HB2 H 2.668 0.003 . 66 . 11 CYS HB3 H 3.208 0.003 . 67 . 12 LEU H H 8.061 0.002 . 68 . 12 LEU HA H 3.971 0.003 . 69 . 12 LEU HB2 H 1.561 0.006 . 70 . 12 LEU HB3 H 2.016 0.004 . 71 . 12 LEU HG H 1.342 0.007 . 72 . 12 LEU HD1 H 0.768 0.003 . 73 . 12 LEU HD2 H 0.854 0.004 . 74 . 13 LYS H H 7.621 0.003 . 75 . 13 LYS HA H 4.473 0.005 . 76 . 13 LYS HB2 H 1.561 0.003 . 77 . 13 LYS HB3 H 1.692 0.003 . 78 . 13 LYS HG2 H 1.396 0.003 . 79 . 13 LYS HD2 H 1.631 0.004 . 80 . 14 TYR H H 8.418 0.002 . 81 . 14 TYR HA H 4.843 0.004 . 82 . 14 TYR HB2 H 2.790 0.008 . 83 . 14 TYR HB3 H 2.846 0.009 . 84 . 14 TYR HD1 H 6.970 0.004 . 85 . 14 TYR HE1 H 6.735 0.003 . 86 . 15 MET H H 9.202 0.001 . 87 . 15 MET HA H 5.104 0.001 . 88 . 15 MET HB2 H 1.895 0.007 . 89 . 15 MET HB3 H 2.038 0.004 . 90 . 15 MET HG2 H 2.200 0.005 . 91 . 15 MET HG3 H 2.606 0.004 . 92 . 16 LYS H H 8.625 0.003 . 93 . 16 LYS HA H 4.300 0.002 . 94 . 16 LYS HB2 H 1.856 0.005 . 95 . 16 LYS HB3 H 1.947 0.002 . 96 . 16 LYS HG2 H 1.496 0.003 . 97 . 16 LYS HG3 H 1.558 0.003 . 98 . 16 LYS HD2 H 1.704 0.003 . 99 . 16 LYS HE2 H 3.027 0.002 . 100 . 17 SER H H 7.243 0.003 . 101 . 17 SER HA H 4.553 0.002 . 102 . 17 SER HB2 H 3.842 0.004 . 103 . 17 SER HB3 H 4.179 0.003 . 104 . 18 ARG H H 8.517 0.001 . 105 . 18 ARG HA H 3.355 0.005 . 106 . 18 ARG HB2 H 1.545 0.008 . 107 . 18 ARG HB3 H 1.654 0.007 . 108 . 18 ARG HG2 H 1.400 0.003 . 109 . 18 ARG HD2 H 3.146 0.007 . 110 . 18 ARG HE H 7.268 0.001 . 111 . 19 THR H H 7.995 0.001 . 112 . 19 THR HA H 3.985 0.001 . 113 . 19 THR HG2 H 1.223 0.005 . 114 . 19 THR HB H 4.013 0.009 . 115 . 20 ILE H H 7.571 0.002 . 116 . 20 ILE HA H 3.796 0.002 . 117 . 20 ILE HB H 1.691 0.004 . 118 . 20 ILE HG2 H 1.070 0.004 . 119 . 20 ILE HG12 H 1.158 0.003 . 120 . 20 ILE HG13 H 1.512 0.007 . 121 . 20 ILE HD1 H 0.902 0.004 . 122 . 21 LEU H H 8.004 0.003 . 123 . 21 LEU HA H 4.178 0.002 . 124 . 21 LEU HB2 H 1.345 0.004 . 125 . 21 LEU HB3 H 2.130 0.003 . 126 . 21 LEU HG H 1.682 0.008 . 127 . 21 LEU HD2 H 1.013 0.004 . 128 . 21 LEU HD1 H 0.986 0.005 . 129 . 22 GLN H H 8.379 0.002 . 130 . 22 GLN HA H 4.015 0.002 . 131 . 22 GLN HB2 H 2.140 0.005 . 132 . 22 GLN HB3 H 2.256 0.002 . 133 . 22 GLN HG2 H 2.371 0.003 . 134 . 22 GLN HG3 H 2.540 0.006 . 135 . 22 GLN HE21 H 7.374 0.005 . 136 . 22 GLN HE22 H 6.849 0.003 . 137 . 23 GLN H H 7.742 0.002 . 138 . 23 GLN HA H 4.060 0.001 . 139 . 23 GLN HB2 H 2.161 0.005 . 140 . 23 GLN HB3 H 2.219 0.004 . 141 . 23 GLN HG2 H 2.474 0.003 . 142 . 23 GLN HG3 H 2.578 0.003 . 143 . 23 GLN HE21 H 7.422 0.003 . 144 . 23 GLN HE22 H 6.851 0.003 . 145 . 24 HIS H H 8.856 0.002 . 146 . 24 HIS HA H 4.265 0.005 . 147 . 24 HIS HB2 H 3.376 0.004 . 148 . 24 HIS HB3 H 3.711 0.004 . 149 . 24 HIS HD2 H 7.166 0.003 . 150 . 24 HIS HE1 H 7.993 0.001 . 151 . 25 MET H H 8.843 0.002 . 152 . 25 MET HA H 4.036 0.003 . 153 . 25 MET HB2 H 2.245 0.006 . 154 . 25 MET HB3 H 2.406 0.007 . 155 . 25 MET HG2 H 3.084 0.004 . 156 . 25 MET HG3 H 3.204 0.002 . 157 . 26 LYS H H 7.206 0.003 . 158 . 26 LYS HA H 4.086 0.004 . 159 . 26 LYS HB2 H 1.892 0.003 . 160 . 26 LYS HG2 H 1.505 0.003 . 161 . 26 LYS HD2 H 1.689 0.006 . 162 . 26 LYS HE2 H 2.962 0.003 . 163 . 27 LYS H H 7.161 0.002 . 164 . 27 LYS HA H 4.398 0.002 . 165 . 27 LYS HB2 H 1.609 0.004 . 166 . 27 LYS HG2 H 1.266 0.011 . 167 . 27 LYS HG3 H 1.364 0.004 . 168 . 27 LYS HD2 H 1.874 0.003 . 169 . 27 LYS HE2 H 2.933 0.003 . 170 . 28 CYS H H 7.368 0.002 . 171 . 28 CYS HA H 4.008 0.003 . 172 . 28 CYS HB2 H 1.984 0.003 . 173 . 28 CYS HB3 H 2.519 0.004 . 174 . 29 GLY H H 7.930 0.001 . 175 . 29 GLY HA2 H 2.967 0.004 . 176 . 29 GLY HA3 H 3.119 0.004 . 177 . 30 TRP H H 7.354 0.006 . 178 . 30 TRP HA H 4.504 0.003 . 179 . 30 TRP HB2 H 3.039 0.005 . 180 . 30 TRP HB3 H 3.158 0.009 . 181 . 30 TRP HD1 H 7.059 0.006 . 182 . 30 TRP HE3 H 7.600 0.003 . 183 . 30 TRP HE1 H 10.015 0.001 . 184 . 30 TRP HZ3 H 7.153 0.002 . 185 . 30 TRP HZ2 H 7.488 0.003 . 186 . 30 TRP HH2 H 7.223 0.010 . 187 . 31 PHE H H 7.472 0.001 . 188 . 31 PHE HA H 4.373 0.001 . 189 . 31 PHE HB2 H 2.886 0.004 . 190 . 31 PHE HB3 H 3.079 0.002 . 191 . 31 PHE HD1 H 7.123 0.004 . 192 . 31 PHE HE1 H 7.255 0.000 . stop_ save_ ######################## # Coupling constants # ######################## save_MOZ_coupling_constants _Saveframe_category coupling_constants _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Spectrometer_frequency_1H 600 _Mol_system_component_name 'Monocytic leukemia zinc finger protein' _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 3JHNHA 20 ILE HA 20 ILE H 4 . . . 2 3JHNHA 21 LEU HA 21 LEU H 4 . . . 3 3JHNHA 22 GLN HA 22 GLN H 4 . . . 4 3JHNHA 23 GLN HA 23 GLN H 4 . . . 5 3JHNHA 24 HIS HA 24 HIS H 4 . . . stop_ save_