data_6112 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C and 15N Chemical Shift Assignments for p27-KID in Solution ; _BMRB_accession_number 6112 _BMRB_flat_file_name bmr6112.str _Entry_type original _Submission_date 2004-02-23 _Accession_date 2004-02-24 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lacy Eilyn R. . 2 Filippov Igor . . 3 Lewis William S. . 4 Otieno Steve . . 5 Xiao Limin . . 6 Weiss Sonja . . 7 Hengst Ludger . . 8 Kriwacki Richard W. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 74 "13C chemical shifts" 82 "15N chemical shifts" 74 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2004-05-15 original author . stop_ _Original_release_date 2004-05-15 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'p27 binds cyclin-CDK complexes through a sequential mechanism involving binding-induced protein folding' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15024385 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lacy Eilyn R. . 2 Filippov Igor . . 3 Lewis William S. . 4 Otieno Steve . . 5 Xiao Limin . . 6 Weiss Sonja . . 7 Hengst Ludger . . 8 Kriwacki Richard W. . stop_ _Journal_abbreviation 'Nat. Struct. Mol. Biol.' _Journal_volume 11 _Journal_issue 4 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 358 _Page_last 364 _Year 2004 _Details . loop_ _Keyword 'intrinsically disordered proteins' stop_ save_ ################################## # Molecular system description # ################################## save_system_p27 _Saveframe_category molecular_system _Mol_system_name 'p27 kinase inhibitory domain' _Abbreviation_common p27-KID _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label p27-KID $p27-KID stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'all free' loop_ _Biological_function 'inhibitor of cyclin dependent kinases' stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_p27-KID _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Cyclin dependent kinase inhibitor 1B' _Abbreviation_common p27 _Molecular_mass 10406 _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 88 _Mol_residue_sequence ; GSHMEHPKPSACRNLFGPVD HEELTRDLEKHCRDMEEASQ RKWNFDFQNHKPLEGKYEWQ EVEKGSLPEFYYRPPRPPKG ACKVPAQE ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 -5 GLY 2 -4 SER 3 -2 HIS 4 -1 MET 5 1 GLU 6 2 HIS 7 3 PRO 8 4 LYS 9 5 PRO 10 6 SER 11 7 ALA 12 8 CYS 13 9 ARG 14 10 ASN 15 11 LEU 16 12 PHE 17 13 GLY 18 14 PRO 19 15 VAL 20 16 ASP 21 17 HIS 22 18 GLU 23 19 GLU 24 20 LEU 25 21 THR 26 22 ARG 27 23 ASP 28 24 LEU 29 25 GLU 30 26 LYS 31 27 HIS 32 28 CYS 33 29 ARG 34 30 ASP 35 31 MET 36 32 GLU 37 33 GLU 38 34 ALA 39 35 SER 40 36 GLN 41 37 ARG 42 38 LYS 43 39 TRP 44 40 ASN 45 41 PHE 46 42 ASP 47 43 PHE 48 44 GLN 49 45 ASN 50 46 HIS 51 47 LYS 52 48 PRO 53 49 LEU 54 50 GLU 55 51 GLY 56 52 LYS 57 53 TYR 58 54 GLU 59 55 TRP 60 56 GLN 61 57 GLU 62 58 VAL 63 59 GLU 64 60 LYS 65 61 GLY 66 62 SER 67 63 LEU 68 64 PRO 69 65 GLU 70 66 PHE 71 67 TYR 72 68 TYR 73 69 ARG 74 70 PRO 75 71 PRO 76 72 ARG 77 73 PRO 78 74 PRO 79 75 LYS 80 76 GLY 81 77 ALA 82 78 CYS 83 79 LYS 84 80 VAL 85 81 PRO 86 82 ALA 87 83 GLN 88 84 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1JSU "P27(Kip1)CYCLIN ACDK2 COMPLEX" 94.32 84 100.00 100.00 7.49e-54 DBJ BAG35368 "unnamed protein product [Homo sapiens]" 97.73 198 97.67 97.67 7.40e-56 DBJ BAG60584 "unnamed protein product [Homo sapiens]" 61.36 147 100.00 100.00 2.38e-31 DBJ BAG70105 "cyclin-dependent kinase inhibitor 1B [Homo sapiens]" 97.73 198 97.67 97.67 7.40e-56 DBJ BAG70237 "cyclin-dependent kinase inhibitor 1B [Homo sapiens]" 97.73 198 97.67 97.67 7.40e-56 DBJ BAG73064 "cyclin-dependent kinase inhibitor 1B [synthetic construct]" 97.73 198 97.67 97.67 7.48e-56 EMBL CAA59284 "p27 kip1 [Homo sapiens]" 97.73 158 97.67 97.67 1.94e-55 EMBL CAE82383 "cyclin-dependent kinase inhibitor 1B [Homo sapiens]" 97.73 136 97.67 97.67 2.81e-55 EMBL CAG33680 "CDKN1B [Homo sapiens]" 97.73 198 97.67 97.67 7.40e-56 EMBL CAL37442 "hypothetical protein [synthetic construct]" 97.73 198 97.67 97.67 7.48e-56 EMBL CAL38417 "hypothetical protein [synthetic construct]" 97.73 198 97.67 97.67 7.48e-56 GB AAA20240 "p27kip1 [Homo sapiens]" 97.73 198 97.67 97.67 7.40e-56 GB AAF69497 "cyclin-dependent kinase inhibitor p27kip1 [Homo sapiens]" 97.73 198 97.67 97.67 8.24e-56 GB AAH01971 "Cyclin-dependent kinase inhibitor 1B (p27, Kip1) [Homo sapiens]" 97.73 198 97.67 97.67 7.48e-56 GB AAL78041 "cyclin-dependent kinase inhibitor 1B (p27, Kip1) [Homo sapiens]" 97.73 198 97.67 97.67 7.40e-56 GB AAV38358 "cyclin-dependent kinase inhibitor 1B (p27, Kip1) [synthetic construct]" 97.73 199 97.67 97.67 7.54e-56 REF NP_004055 "cyclin-dependent kinase inhibitor 1B [Homo sapiens]" 97.73 198 97.67 97.67 7.40e-56 REF XP_002822998 "PREDICTED: cyclin-dependent kinase inhibitor 1B [Pongo abelii]" 97.73 198 97.67 97.67 7.40e-56 REF XP_003808898 "PREDICTED: cyclin-dependent kinase inhibitor 1B [Pan paniscus]" 97.73 198 97.67 97.67 7.40e-56 REF XP_004052799 "PREDICTED: cyclin-dependent kinase inhibitor 1B [Gorilla gorilla gorilla]" 97.73 198 97.67 97.67 7.40e-56 REF XP_005680873 "PREDICTED: cyclin-dependent kinase inhibitor 1B [Capra hircus]" 61.36 147 98.15 100.00 1.64e-30 SP P46527 "RecName: Full=Cyclin-dependent kinase inhibitor 1B; AltName: Full=Cyclin-dependent kinase inhibitor p27; AltName: Full=p27Kip1 " 97.73 198 97.67 97.67 7.40e-56 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $p27-KID Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Cell_line _Vector_type _Vector_name $p27-KID 'recombinant technology' 'E. coli' Escherichia Coli . BL21(DE3) plasmid pET28a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_p27KID _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $p27-KID 1.0 mM 0.8 1.2 '[U-2H; U-13C; U-15N]' stop_ save_ ############################ # Computer software used # ############################ save_Felix _Saveframe_category software _Name Felix _Version . _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Inova _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_CT-HNCA_1 _Saveframe_category NMR_applied_experiment _Experiment_name CT-HNCA _Sample_label $p27KID save_ save_CT-HN(CO)CA_2 _Saveframe_category NMR_applied_experiment _Experiment_name CT-HN(CO)CA _Sample_label $p27KID save_ save_CT-HN(CA)CB_3 _Saveframe_category NMR_applied_experiment _Experiment_name CT-HN(CA)CB _Sample_label $p27KID save_ save_CT-HN(COCA)CB_4 _Saveframe_category NMR_applied_experiment _Experiment_name CT-HN(COCA)CB _Sample_label $p27KID save_ ####################### # Sample conditions # ####################### save_Cond_set_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 0.1 pH temperature 298 0.1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label TSP H 1 'methyl protons' ppm 0.0 external indirect spherical external parallel 1.0 $entry_citation $entry_citation TSP N 15 'methyl protons' ppm 0.0 external indirect spherical external parallel 0.101383 $entry_citation $entry_citation TSP C 13 'methyl protons' ppm 0.0 external indirect spherical external parallel 0.251449 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $p27KID stop_ _Sample_conditions_label $Cond_set_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name p27-KID _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 5 GLU H H 8.4 . 1 2 1 5 GLU CA C 56.36 . 1 3 1 5 GLU N N 123.53 . 1 4 2 6 HIS H H 8.46 . 1 5 2 6 HIS CA C 53.73 . 1 6 2 6 HIS N N 122.1 . 1 7 3 7 PRO CA C 63.37 . 1 8 4 8 LYS H H 8.55 . 1 9 4 8 LYS CA C 52.51 . 1 10 4 8 LYS N N 124.24 . 1 11 5 9 PRO CA C 63.41 . 1 12 6 10 SER H H 8.42 . 1 13 6 10 SER CA C 58.52 . 1 14 6 10 SER N N 117.06 . 1 15 7 11 ALA H H 8.39 . 1 16 7 11 ALA CA C 52.65 . 1 17 7 11 ALA N N 126.95 . 1 18 8 12 CYS H H 8.27 . 1 19 8 12 CYS CA C 58.61 . 1 20 8 12 CYS N N 119.22 . 1 21 9 13 ARG H H 8.38 . 1 22 9 13 ARG CA C 56.32 . 1 23 9 13 ARG N N 124.28 . 1 24 10 14 ASN H H 8.44 . 1 25 10 14 ASN CA C 53.23 . 1 26 10 14 ASN N N 120.66 . 1 27 11 15 LEU H H 8.13 . 1 28 11 15 LEU CA C 55.5 . 1 29 11 15 LEU N N 123.54 . 1 30 12 16 PHE H H 8.15 . 1 31 12 16 PHE CA C 57.31 . 1 32 12 16 PHE N N 120.29 . 1 33 13 17 GLY H H 8.08 . 1 34 13 17 GLY CA C 44.51 . 1 35 13 17 GLY N N 110.94 . 1 36 14 18 PRO CA C 63.14 . 1 37 15 19 VAL H H 8.24 . 1 38 15 19 VAL CA C 62.14 . 1 39 15 19 VAL N N 120.82 . 1 40 16 20 ASP H H 8.36 . 1 41 16 20 ASP CA C 54.24 . 1 42 16 20 ASP N N 124.61 . 1 43 17 21 HIS H H 8.39 . 1 44 17 21 HIS CA C 56.57 . 1 45 17 21 HIS N N 120.47 . 1 46 18 22 GLU H H 8.51 . 1 47 18 22 GLU CA C 57.44 . 1 48 18 22 GLU N N 122.95 . 1 49 19 23 GLU H H 8.51 . 1 50 19 23 GLU CA C 57.3 . 1 51 19 23 GLU N N 122.81 . 1 52 20 24 LEU H H 8.21 . 1 53 20 24 LEU CA C 55.97 . 1 54 20 24 LEU N N 123.17 . 1 55 21 25 THR H H 8.01 . 1 56 21 25 THR CA C 62.85 . 1 57 21 25 THR N N 114.9 . 1 58 22 26 ARG H H 8.17 . 1 59 22 26 ARG CA C 57.14 . 1 60 22 26 ARG N N 123.73 . 1 61 23 27 ASP H H 8.33 . 1 62 23 27 ASP CA C 55.13 . 1 63 23 27 ASP N N 121.74 . 1 64 24 28 LEU H H 8.17 . 1 65 24 28 LEU CA C 56.44 . 1 66 24 28 LEU N N 123.36 . 1 67 25 29 GLU H H 8.3 . 1 68 25 29 GLU CA C 57.69 . 1 69 25 29 GLU N N 121.19 . 1 70 26 30 LYS H H 8.04 . 1 71 26 30 LYS CA C 57.39 . 1 72 26 30 LYS N N 121.73 . 1 73 27 31 HIS H H 8.29 . 1 74 27 31 HIS CA C 56.68 . 1 75 27 31 HIS N N 119.75 . 1 76 28 32 CYS H H 8.3 . 1 77 28 32 CYS CA C 59.95 . 1 78 28 32 CYS N N 120.65 . 1 79 29 33 ARG H H 8.44 . 1 80 29 33 ARG CA C 57.31 . 1 81 29 33 ARG N N 123.9 . 1 82 30 34 ASP H H 8.37 . 1 83 30 34 ASP CA C 55.46 . 1 84 30 34 ASP N N 121.55 . 1 85 31 35 MET H H 8.21 . 1 86 31 35 MET CA C 56.43 . 1 87 31 35 MET N N 120.84 . 1 88 32 36 GLU H H 8.3 . 1 89 32 36 GLU CA C 57.73 . 1 90 32 36 GLU N N 122.81 . 1 91 33 37 GLU H H 8.4 . 1 92 33 37 GLU CA C 57.87 . 1 93 33 37 GLU N N 122.45 . 1 94 34 38 ALA H H 8.26 . 1 95 34 38 ALA CA C 53.9 . 1 96 34 38 ALA N N 124.25 . 1 97 35 39 SER H H 8.12 . 1 98 35 39 SER CA C 59.65 . 1 99 35 39 SER N N 114.73 . 1 100 36 40 GLN H H 8.08 . 1 101 36 40 GLN CA C 56.54 . 1 102 36 40 GLN N N 121.91 . 1 103 37 41 ARG H H 8.01 . 1 104 37 41 ARG CA C 56.76 . 1 105 37 41 ARG N N 121.54 . 1 106 38 42 LYS H H 8.05 . 1 107 38 42 LYS CA C 56.74 . 1 108 38 42 LYS N N 122.22 . 1 109 39 43 TRP H H 8.05 . 1 110 39 43 TRP CA C 57.31 . 1 111 39 43 TRP N N 121.92 . 1 112 40 44 ASN H H 8.13 . 1 113 40 44 ASN CA C 53.24 . 1 114 40 44 ASN N N 120.65 . 1 115 41 45 PHE H H 7.96 . 1 116 41 45 PHE CA C 58.28 . 1 117 41 45 PHE N N 121.37 . 1 118 42 46 ASP H H 8.16 . 1 119 42 46 ASP CA C 54.33 . 1 120 42 46 ASP N N 122.45 . 1 121 43 47 PHE H H 8.05 . 1 122 43 47 PHE CA C 58.65 . 1 123 43 47 PHE N N 121.55 . 1 124 44 48 GLN H H 8.18 . 1 125 44 48 GLN CA C 56.49 . 1 126 44 48 GLN N N 120.66 . 1 127 45 49 ASN H H 8.1 . 1 128 45 49 ASN CA C 53.28 . 1 129 45 49 ASN N N 118.85 . 1 130 46 50 HIS H H 8.14 . 1 131 46 50 HIS CA C 55.71 . 1 132 46 50 HIS N N 119.94 . 1 133 47 51 LYS H H 8.21 . 1 134 47 51 LYS CA C 54.36 . 1 135 47 51 LYS N N 124.48 . 1 136 48 52 PRO CA C 63.23 . 1 137 49 53 LEU H H 8.33 . 1 138 49 53 LEU CA C 55.32 . 1 139 49 53 LEU N N 123.15 . 1 140 50 54 GLU H H 8.36 . 1 141 50 54 GLU CA C 56.63 . 1 142 50 54 GLU N N 122.25 . 1 143 51 55 GLY H H 8.36 . 1 144 51 55 GLY CA C 45.36 . 1 145 51 55 GLY N N 110.94 . 1 146 52 56 LYS H H 7.97 . 1 147 52 56 LYS CA C 56.09 . 1 148 52 56 LYS N N 121.54 . 1 149 53 57 TYR H H 8.17 . 1 150 53 57 TYR CA C 57.57 . 1 151 53 57 TYR N N 122.09 . 1 152 54 58 GLU H H 8.4 . 1 153 54 58 GLU CA C 56.72 . 1 154 54 58 GLU N N 123.53 . 1 155 55 59 TRP H H 7.97 . 1 156 55 59 TRP CA C 57.48 . 1 157 55 59 TRP N N 122.27 . 1 158 56 60 GLN H H 7.96 . 1 159 56 60 GLN CA C 56.01 . 1 160 56 60 GLN N N 122.32 . 1 161 57 61 GLU H H 8.21 . 1 162 57 61 GLU CA C 56.78 . 1 163 57 61 GLU N N 122.63 . 1 164 58 62 VAL H H 8.01 . 1 165 58 62 VAL CA C 62.58 . 1 166 58 62 VAL N N 121.55 . 1 167 59 63 GLU H H 8.39 . 1 168 59 63 GLU CA C 56.79 . 1 169 59 63 GLU N N 124.81 . 1 170 60 64 LYS H H 8.33 . 1 171 60 64 LYS CA C 57.05 . 1 172 60 64 LYS N N 123.53 . 1 173 61 65 GLY H H 8.41 . 1 174 61 65 GLY CA C 45.38 . 1 175 61 65 GLY N N 110.58 . 1 176 62 66 SER H H 8.12 . 1 177 62 66 SER CA C 58.25 . 1 178 62 66 SER N N 116.34 . 1 179 63 67 LEU H H 8.13 . 1 180 63 67 LEU CA C 53.16 . 1 181 63 67 LEU N N 125.52 . 1 182 64 68 PRO CA C 63.41 . 1 183 65 69 GLU H H 8.5 . 1 184 65 69 GLU CA C 57.45 . 1 185 65 69 GLU N N 121.02 . 1 186 66 70 PHE H H 7.95 . 1 187 66 70 PHE CA C 57.78 . 1 188 66 70 PHE N N 119.58 . 1 189 67 71 TYR H H 7.83 . 1 190 67 71 TYR CA C 58.13 . 1 191 67 71 TYR N N 121.73 . 1 192 68 72 TYR H H 7.97 . 1 193 68 72 TYR CA C 57.89 . 1 194 68 72 TYR N N 122.63 . 1 195 69 73 ARG H H 7.87 . 1 196 69 73 ARG CA C 53.1 . 1 197 69 73 ARG N N 126.05 . 1 198 71 75 PRO CA C 63.39 . 1 199 72 76 ARG H H 8.35 . 1 200 72 76 ARG CA C 53.24 . 1 201 72 76 ARG N N 122.99 . 1 202 74 78 PRO CA C 62.83 . 1 203 75 79 LYS H H 8.43 . 1 204 75 79 LYS CA C 56.78 . 1 205 75 79 LYS N N 122.63 . 1 206 76 80 GLY H H 8.47 . 1 207 76 80 GLY CA C 45.16 . 1 208 76 80 GLY N N 111.48 . 1 209 77 81 ALA H H 8.18 . 1 210 77 81 ALA CA C 52.4 . 1 211 77 81 ALA N N 124.78 . 1 212 78 82 CYS H H 8.35 . 1 213 78 82 CYS CA C 58.41 . 1 214 78 82 CYS N N 119.76 . 1 215 79 83 LYS H H 8.47 . 1 216 79 83 LYS CA C 56.35 . 1 217 79 83 LYS N N 125.33 . 1 218 80 84 VAL H H 8.21 . 1 219 80 84 VAL CA C 59.81 . 1 220 80 84 VAL N N 124.08 . 1 221 81 85 PRO CA C 62.85 . 1 222 82 86 ALA H H 8.36 . 1 223 82 86 ALA CA C 52.86 . 1 224 82 86 ALA N N 125.33 . 1 225 83 87 GLN H H 8.35 . 1 226 83 87 GLN CA C 55.58 . 1 227 83 87 GLN N N 121.16 . 1 228 84 88 GLU H H 8.07 . 1 229 84 88 GLU CA C 55.33 . 1 230 84 88 GLU N N 128.92 . 1 stop_ save_