data_6216 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of the third zinc finger domain of FOG-1 ; _BMRB_accession_number 6216 _BMRB_flat_file_name bmr6216.str _Entry_type original _Submission_date 2004-05-28 _Accession_date 2004-05-28 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Simpson R. J.Y. . 2 Lee S. H.Y. . 3 Bartle N. . . 4 Matthews J. M. . 5 Mackay J. P. . 6 Crossley M. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 coupling_constants 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 180 "coupling constants" 31 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2005-02-08 original author . stop_ _Original_release_date 2005-02-08 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3 ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15234987 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Simpson R. J. . 2 Lee S. H.Y. . 3 Bartle N. . . 4 Sum E. Y. . 5 Visvader J. E. . 6 Matthews J. M. . 7 Mackay J. P. . 8 Crossley M. . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 279 _Journal_issue 38 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 39789 _Page_last 39797 _Year 2004 _Details . loop_ _Keyword 'classical zinc finger' stop_ save_ ################################## # Molecular system description # ################################## save_system_ZFPM1 _Saveframe_category molecular_system _Mol_system_name 'Zinc finger protein ZFPM1' _Abbreviation_common 'Zinc finger protein ZFPM1' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Zinc finger protein ZFPM1' $ZFPM1 'ZINC (II) ION' $ZN stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'other bound and free' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_ZFPM1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common ZFPM1 _Name_variant FOG-1 _Abbreviation_common ZFPM1 _Molecular_mass . _Mol_thiol_state 'other bound and free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 35 _Mol_residue_sequence ; GSSGKRPFVCRICLSAFTTK ANCARHLKVHTDTLS ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 SER 3 SER 4 GLY 5 LYS 6 ARG 7 PRO 8 PHE 9 VAL 10 CYS 11 ARG 12 ILE 13 CYS 14 LEU 15 SER 16 ALA 17 PHE 18 THR 19 THR 20 LYS 21 ALA 22 ASN 23 CYS 24 ALA 25 ARG 26 HIS 27 LEU 28 LYS 29 VAL 30 HIS 31 THR 32 ASP 33 THR 34 LEU 35 SER stop_ _Sequence_homology_query_date 2008-08-19 _Sequence_homology_query_revised_last_date 2008-08-19 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1SRK 'Solution Structure Of The Third Zinc Finger Domain Of Fog-1' 100.00 35 100.00 100.00 9.06e-12 stop_ save_ ############# # Ligands # ############# save_ZN _Saveframe_category ligand _Mol_type non-polymer _Name_common "ZN (ZINC ION)" _BMRB_code . _PDB_code ZN _Molecular_mass 65.409 _Mol_charge 2 _Mol_paramagnetic . _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Thu Jun 2 09:48:05 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons ZN ZN ZN . 2 . ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $ZFPM1 Mouse 10090 Eukaryota Metazoa Mus musculus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $ZFPM1 'recombinant technology' 'E. coli' Escherichia coli . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $ZFPM1 0.27 mM . ZnSO4 1 mM . TCEP 2 mM . H2O 95 % . D2O 5 % . stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $ZFPM1 0.245 mM [U-15N] ZnSO4 1 mM . TCEP 2 mM . H2O 95 % . D2O 5 % . stop_ save_ ############################ # Computer software used # ############################ save_XWINNMR _Saveframe_category software _Name xwinnmr _Version 2.6 loop_ _Task processing stop_ _Details Bruker save_ save_XEASY _Saveframe_category software _Name XEASY _Version 1.3.13 loop_ _Task 'data analysis' stop_ _Details 'Bartels et al' save_ save_DYANA _Saveframe_category software _Name DYANA _Version 1.5 loop_ _Task refinement stop_ _Details 'Guntert et al' save_ save_ARIA _Saveframe_category software _Name ARIA _Version 1.2 loop_ _Task 'structure solution' stop_ _Details 'Linge et al' save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label . save_ save_2D_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _Sample_label . save_ save_DQF-COSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _Sample_label . save_ save_HNHA_4 _Saveframe_category NMR_applied_experiment _Experiment_name HNHA _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name HNHA _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0 . mM pH 5.6 . pH pressure 1 . atm temperature 275 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 $sample_2 stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'Zinc finger protein ZFPM1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 2 SER H H 8.851 0.000 1 2 . 2 SER HA H 4.554 0.001 1 3 . 2 SER HB2 H 3.906 0.002 1 4 . 2 SER HB3 H 3.853 0.001 1 5 . 3 SER H H 8.717 0.001 1 6 . 3 SER HA H 4.497 0.002 1 7 . 3 SER HB2 H 3.909 0.003 1 8 . 3 SER HB3 H 3.880 0.001 1 9 . 4 GLY H H 8.574 0.001 1 10 . 4 GLY HA2 H 3.932 0.000 2 11 . 5 LYS H H 8.314 0.001 1 12 . 5 LYS HA H 4.209 0.003 1 13 . 5 LYS HB2 H 1.737 0.000 1 14 . 5 LYS HB3 H 1.665 0.001 1 15 . 5 LYS HG2 H 1.360 0.000 2 16 . 5 LYS HD2 H 1.446 0.000 2 17 . 6 ARG H H 8.438 0.001 1 18 . 6 ARG HA H 4.531 0.003 1 19 . 6 ARG HB2 H 1.520 0.002 1 20 . 6 ARG HB3 H 1.460 0.004 1 21 . 6 ARG HG2 H 1.335 0.003 1 22 . 6 ARG HG3 H 1.286 0.001 1 23 . 6 ARG HD2 H 2.992 0.005 2 24 . 6 ARG HE H 7.091 0.001 1 25 . 7 PRO HA H 4.268 0.002 1 26 . 7 PRO HB2 H 1.970 0.003 1 27 . 7 PRO HB3 H 1.120 0.001 1 28 . 7 PRO HG2 H 1.711 0.003 1 29 . 7 PRO HG3 H 1.532 0.003 1 30 . 7 PRO HD2 H 3.615 0.003 1 31 . 7 PRO HD3 H 3.566 0.007 1 32 . 8 PHE H H 7.901 0.002 1 33 . 8 PHE HA H 4.664 0.004 1 34 . 8 PHE HB2 H 3.021 0.001 1 35 . 8 PHE HB3 H 2.737 0.007 1 36 . 8 PHE HZ H 7.389 0.000 1 37 . 8 PHE HD1 H 7.145 0.002 3 38 . 8 PHE HE1 H 7.419 0.003 3 39 . 9 VAL H H 8.644 0.001 1 40 . 9 VAL HA H 4.618 0.002 1 41 . 9 VAL HB H 1.854 0.002 1 42 . 9 VAL HG1 H 0.819 0.003 1 43 . 9 VAL HG2 H 0.758 0.002 1 44 . 10 CYS H H 9.404 0.001 1 45 . 10 CYS HA H 4.356 0.003 1 46 . 10 CYS HB2 H 3.424 0.002 1 47 . 10 CYS HB3 H 3.041 0.002 1 48 . 11 ARG H H 9.068 0.001 1 49 . 11 ARG HA H 4.209 0.003 1 50 . 11 ARG HB2 H 1.942 0.002 2 51 . 11 ARG HG2 H 1.756 0.004 1 52 . 11 ARG HG3 H 1.653 0.003 1 53 . 11 ARG HD2 H 3.232 0.004 2 54 . 11 ARG HE H 7.390 0.002 1 55 . 12 ILE H H 8.834 0.001 1 56 . 12 ILE HA H 3.865 0.003 1 57 . 12 ILE HB H 1.160 0.006 1 58 . 12 ILE HG2 H 0.280 0.004 1 59 . 12 ILE HG12 H 1.341 0.001 1 60 . 12 ILE HG13 H 1.068 0.002 1 61 . 12 ILE HD1 H 0.681 0.004 1 62 . 13 CYS H H 8.394 0.002 1 63 . 13 CYS HA H 5.130 0.002 1 64 . 13 CYS HB2 H 3.518 0.002 1 65 . 13 CYS HB3 H 2.836 0.003 1 66 . 14 LEU H H 8.267 0.001 1 67 . 14 LEU HA H 4.230 0.003 1 68 . 14 LEU HB2 H 2.219 0.005 1 69 . 14 LEU HB3 H 1.617 0.005 1 70 . 14 LEU HG H 1.394 0.005 1 71 . 14 LEU HD1 H 0.874 0.002 1 72 . 14 LEU HD2 H 0.819 0.003 1 73 . 15 SER H H 8.317 0.001 1 74 . 15 SER HA H 4.232 0.001 1 75 . 15 SER HB2 H 3.502 0.003 1 76 . 15 SER HB3 H 3.409 0.002 1 77 . 16 ALA H H 8.238 0.001 1 78 . 16 ALA HA H 5.113 0.003 1 79 . 16 ALA HB H 1.270 0.001 1 80 . 17 PHE H H 9.061 0.001 1 81 . 17 PHE HA H 4.774 0.003 1 82 . 17 PHE HB2 H 3.383 0.004 1 83 . 17 PHE HB3 H 2.851 0.005 1 84 . 17 PHE HZ H 6.177 0.004 1 85 . 17 PHE HD1 H 7.294 0.002 3 86 . 17 PHE HE1 H 6.860 0.004 3 87 . 18 THR H H 9.432 0.001 1 88 . 18 THR HA H 4.523 0.002 1 89 . 18 THR HB H 4.518 0.007 1 90 . 18 THR HG2 H 1.438 0.003 1 91 . 19 THR H H 7.222 0.001 1 92 . 19 THR HA H 4.714 0.001 1 93 . 19 THR HB H 4.419 0.001 1 94 . 19 THR HG2 H 1.251 0.000 1 95 . 19 THR HG1 H 5.509 0.002 1 96 . 20 LYS H H 8.195 0.001 1 97 . 20 LYS HA H 3.060 0.002 1 98 . 20 LYS HB2 H 1.395 0.004 1 99 . 20 LYS HB3 H 1.170 0.003 1 100 . 20 LYS HG2 H 1.093 0.002 1 101 . 20 LYS HD2 H 1.596 0.002 1 102 . 20 LYS HE2 H 2.962 0.001 2 103 . 20 LYS HZ H 7.673 0.001 1 104 . 21 ALA H H 8.558 0.001 1 105 . 21 ALA HA H 4.057 0.002 1 106 . 21 ALA HB H 1.302 0.002 1 107 . 22 ASN H H 8.062 0.001 1 108 . 22 ASN HA H 4.418 0.002 1 109 . 22 ASN HB2 H 2.912 0.002 1 110 . 22 ASN HB3 H 2.884 0.000 1 111 . 22 ASN HD21 H 7.520 0.001 1 112 . 22 ASN HD22 H 7.221 0.001 1 113 . 23 CYS H H 7.586 0.001 1 114 . 23 CYS HA H 3.020 0.004 1 115 . 23 CYS HB2 H 3.123 0.001 1 116 . 23 CYS HB3 H 2.542 0.001 1 117 . 23 CYS HG H 1.879 0.000 1 118 . 24 ALA H H 8.449 0.002 1 119 . 24 ALA HA H 4.058 0.003 1 120 . 24 ALA HB H 1.392 0.001 1 121 . 25 ARG H H 7.840 0.001 1 122 . 25 ARG HA H 3.966 0.004 1 123 . 25 ARG HB2 H 1.838 0.003 1 124 . 25 ARG HB3 H 1.775 0.006 1 125 . 25 ARG HG2 H 1.687 0.002 1 126 . 25 ARG HG3 H 1.564 0.002 1 127 . 25 ARG HD2 H 3.204 0.003 2 128 . 25 ARG HE H 7.292 0.003 1 129 . 26 HIS H H 7.277 0.002 1 130 . 26 HIS HA H 4.155 0.002 1 131 . 26 HIS HB2 H 2.928 0.004 1 132 . 26 HIS HB3 H 2.664 0.002 1 133 . 26 HIS HD2 H 6.985 0.001 1 134 . 26 HIS HE1 H 7.994 0.001 1 135 . 27 LEU H H 8.191 0.001 1 136 . 27 LEU HA H 3.867 0.001 1 137 . 27 LEU HB2 H 1.941 0.003 1 138 . 27 LEU HB3 H 1.761 0.003 1 139 . 27 LEU HG H 2.003 0.001 1 140 . 27 LEU HD1 H 1.158 0.004 2 141 . 28 LYS H H 7.035 0.002 1 142 . 28 LYS HA H 4.086 0.003 1 143 . 28 LYS HB2 H 1.893 0.004 1 144 . 28 LYS HB3 H 1.767 0.002 1 145 . 28 LYS HG2 H 1.581 0.004 1 146 . 28 LYS HG3 H 1.472 0.005 1 147 . 28 LYS HD2 H 1.678 0.001 2 148 . 28 LYS HE2 H 2.981 0.003 2 149 . 28 LYS HZ H 7.664 0.001 1 150 . 29 VAL H H 8.007 0.002 1 151 . 29 VAL HA H 3.897 0.004 1 152 . 29 VAL HB H 1.964 0.002 1 153 . 29 VAL HG1 H 0.681 0.002 1 154 . 29 VAL HG2 H 0.540 0.002 1 155 . 30 HIS H H 7.373 0.002 1 156 . 30 HIS HA H 4.791 0.003 1 157 . 30 HIS HB2 H 3.335 0.003 1 158 . 30 HIS HB3 H 3.244 0.002 1 159 . 30 HIS HD2 H 6.830 0.003 1 160 . 30 HIS HE1 H 8.132 0.001 1 161 . 31 THR H H 7.980 0.001 1 162 . 31 THR HA H 4.313 0.005 1 163 . 31 THR HB H 4.307 0.005 1 164 . 31 THR HG2 H 1.224 0.002 1 165 . 32 ASP H H 8.587 0.001 1 166 . 32 ASP HA H 4.676 0.000 1 167 . 32 ASP HB2 H 2.718 0.002 2 168 . 33 THR H H 8.098 0.001 1 169 . 33 THR HA H 4.344 0.002 1 170 . 33 THR HB H 4.260 0.001 1 171 . 33 THR HG2 H 1.224 0.001 1 172 . 34 LEU H H 8.414 0.000 1 173 . 34 LEU HA H 4.436 0.003 1 174 . 34 LEU HB2 H 1.653 0.003 2 175 . 34 LEU HG H 1.714 0.004 1 176 . 34 LEU HD1 H 0.934 0.002 1 177 . 34 LEU HD2 H 0.873 0.003 1 178 . 35 SER H H 8.023 0.001 1 179 . 35 SER HA H 4.260 0.004 1 180 . 35 SER HB2 H 3.839 0.003 2 stop_ save_ ######################## # Coupling constants # ######################## save_coupling_constants_set_1 _Saveframe_category coupling_constants _Details . loop_ _Sample_label $sample_1 $sample_2 stop_ _Sample_conditions_label $sample_cond_1 _Spectrometer_frequency_1H 600 _Mol_system_component_name 'Zinc finger protein ZFPM1' _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 3JHNHA 3 SER H 3 SER HA 7.9 . . . 2 3JHNHA 5 LYS H 5 LYS HA 7.5 . . . 3 3JHNHA 6 ARG H 6 ARG HA 10.4 . . . 4 3JHNHA 8 PHE H 8 PHE HA 10.1 . . . 5 3JHNHA 9 VAL H 9 VAL HA 9.7 . . . 6 3JHNHA 10 CYS H 10 CYS HA 5.4 . . . 7 3JHNHA 11 ARG H 11 ARG HA 3.8 . . . 8 3JHNHA 12 ILE H 12 ILE HA 9.8 . . . 9 3JHNHA 13 CYS H 13 CYS HA 11.0 . . . 10 3JHNHA 14 LEU H 14 LEU HA 8.9 . . . 11 3JHNHA 15 SER H 15 SER HA 6.6 . . . 12 3JHNHA 16 ALA H 16 ALA HA 10.1 . . . 13 3JHNHA 17 PHE H 17 PHE HA 11.2 . . . 14 3JHNHA 18 THR H 18 THR HA 6.0 . . . 15 3JHNHA 19 THR H 19 THR HA 10.2 . . . 16 3JHNHA 20 LYS H 20 LYS HA 5.9 . . . 17 3JHNHA 21 ALA H 21 ALA HA 3.4 . . . 18 3JHNHA 22 ASN H 22 ASN HA 6.1 . . . 19 3JHNHA 23 CYS H 23 CYS HA 5.3 . . . 20 3JHNHA 24 ALA H 24 ALA HA 4.9 . . . 21 3JHNHA 25 ARG H 25 ARG HA 5.1 . . . 22 3JHNHA 26 HIS H 26 HIS HA 6.5 . . . 23 3JHNHA 27 LEU H 27 LEU HA 2.3 . . . 24 3JHNHA 28 LYS H 28 LYS HA 4.8 . . . 25 3JHNHA 29 VAL H 29 VAL HA 6.0 . . . 26 3JHNHA 30 HIS H 30 HIS HA 8.9 . . . 27 3JHNHA 31 THR H 31 THR HA 7.8 . . . 28 3JHNHA 32 ASP H 32 ASP HA 7.3 . . . 29 3JHNHA 33 THR H 33 THR HA 8.8 . . . 30 3JHNHA 34 LEU H 34 LEU HA 8.3 . . . 31 3JHNHA 35 SER H 35 SER HA 8.8 . . . stop_ save_