data_6415 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Three-disulfide variant of hen lysozyme: C30A/C115A ; _BMRB_accession_number 6415 _BMRB_flat_file_name bmr6415.str _Entry_type original _Submission_date 2004-12-07 _Accession_date 2004-12-08 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Yokota Atsushi . . 2 Hirai Kenichi . . 3 Miyauchi Hiroyo . . 4 Iimura Satoshi . . 5 Noda Yasuo . . 6 Inoue Kyouko . . 7 Akasaka Kazuyuki . . 8 Tachibana Hideki . . 9 Segawa Shin-ichi . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 632 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2005-04-04 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 5068 'Recombinant hen lysozyme with extra N-terminal Met' 5069 'Two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]' 5803 'Three-disulfide variant of hen lysozyme: C64A/C80A' 5804 'Three-disulfide variant of hen lysozyme: C76A/C94A' stop_ _Original_release_date 2005-04-04 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; NMR Characterization of Three-Disulfide Variants of Lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A -A Marginally Stable State in Folded Proteins ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15157100 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Yokota Atsushi . . 2 Hirai Kenichi . . 3 Miyauchi Hiroyo . . 4 Iimura Satoshi . . 5 Noda Yasuo . . 6 Inoue Kyouko . . 7 Akasaka Kazuyuki . . 8 Tachibana Hideki . . 9 Segawa Shin-ichi . . stop_ _Journal_abbreviation Biochemistry _Journal_volume 43 _Journal_issue 21 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 6663 _Page_last 6669 _Year 2004 _Details . loop_ _Keyword 'Lysozyme Variants' NMR 'Protein Folding' 'Three-Disulfide Intermediate' stop_ save_ ####################################### # Cited references within the entry # ####################################### save_ref_1 _Saveframe_category citation _Citation_full ; van den Berg B, Chung EW, Robinson CV, Dobson CM. Characterisation of the dominant oxidative folding intermediate of hen lysozyme. J Mol Biol. 1999 Jul 16;290(3):781-96. ; _Citation_title . _Citation_status . _Citation_type . _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 10395829 _Journal_abbreviation . _Journal_name_full . _Journal_volume . _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first . _Page_last . _Year . _Details . save_ save_ref_2 _Saveframe_category citation _Citation_full ; Noda Y, Yokota A, Horii D, Tominaga T, Tanisaka Y, Tachibana H, Segawa S. NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure. Biochemistry. 2002 Feb 19;41(7):2130-9. ; _Citation_title . _Citation_status . _Citation_type . _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 11841203 _Journal_abbreviation . _Journal_name_full . _Journal_volume . _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_HEWL _Saveframe_category molecular_system _Mol_system_name 'hen egg white lysozyme' _Abbreviation_common HEWL _Enzyme_commission_number 3.2.1.17 loop_ _Mol_system_component_name _Mol_label 'hen lysozyme' $C30A_C115A stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'all disulfide bound' loop_ _Biological_function Hydrolase(O-Glycosyl) stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_C30A_C115A _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'hen egg white lysozyme variant' _Name_variant C30A/C115A _Abbreviation_common C30A/C115A _Molecular_mass 14374 _Mol_thiol_state 'all disulfide bound' _Details ; hen lysozyme with Cys30 and Cys115 replaced by Ala and three disulfide bridges between Cys6 and Cys127, between Cys64 and Cys80, and between Cys76 and Cys94 ; ############################## # Polymer residue sequence # ############################## _Residue_count 130 _Mol_residue_sequence ; MKVFGRCELAAAMKRHGLDN YRGYSLGNWVAAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWCNDGRTPGSRNLCNIP CSALLSSDITASVNCAKKIV SDGNGMNAWVAWRNRAKGTD VQAWIRGCRL ; loop_ _Residue_seq_code _Residue_label 1 MET 2 LYS 3 VAL 4 PHE 5 GLY 6 ARG 7 CYS 8 GLU 9 LEU 10 ALA 11 ALA 12 ALA 13 MET 14 LYS 15 ARG 16 HIS 17 GLY 18 LEU 19 ASP 20 ASN 21 TYR 22 ARG 23 GLY 24 TYR 25 SER 26 LEU 27 GLY 28 ASN 29 TRP 30 VAL 31 ALA 32 ALA 33 ALA 34 LYS 35 PHE 36 GLU 37 SER 38 ASN 39 PHE 40 ASN 41 THR 42 GLN 43 ALA 44 THR 45 ASN 46 ARG 47 ASN 48 THR 49 ASP 50 GLY 51 SER 52 THR 53 ASP 54 TYR 55 GLY 56 ILE 57 LEU 58 GLN 59 ILE 60 ASN 61 SER 62 ARG 63 TRP 64 TRP 65 CYS 66 ASN 67 ASP 68 GLY 69 ARG 70 THR 71 PRO 72 GLY 73 SER 74 ARG 75 ASN 76 LEU 77 CYS 78 ASN 79 ILE 80 PRO 81 CYS 82 SER 83 ALA 84 LEU 85 LEU 86 SER 87 SER 88 ASP 89 ILE 90 THR 91 ALA 92 SER 93 VAL 94 ASN 95 CYS 96 ALA 97 LYS 98 LYS 99 ILE 100 VAL 101 SER 102 ASP 103 GLY 104 ASN 105 GLY 106 MET 107 ASN 108 ALA 109 TRP 110 VAL 111 ALA 112 TRP 113 ARG 114 ASN 115 ARG 116 ALA 117 LYS 118 GLY 119 THR 120 ASP 121 VAL 122 GLN 123 ALA 124 TRP 125 ILE 126 ARG 127 GLY 128 CYS 129 ARG 130 LEU stop_ _Sequence_homology_query_date 2008-08-19 _Sequence_homology_query_revised_last_date 2008-08-19 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value GenBank AAL69327 'egg white lysozyme [Gallus gallus]' 99.23 147 98.45 98.45 2.46e-68 SWISS-PROT P00698 'Lysozyme C precursor (1,4-beta-N-acetylmuramidase C) (Allergen Gal d 4) (Allergen Gal d IV)' 99.23 147 98.45 98.45 2.46e-68 PDB 8LYZ 'An X-Ray Study Of The Structure And Binding Properties Of Iodine-Inactivated Lysozyme' 99.23 129 98.45 98.45 5.95e-68 GenBank AAA48943 'lysozyme (' 99.23 147 98.45 98.45 2.46e-68 PDB 6LYZ 'Real-Space Refinement Of The Structure Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 7LYZ 'Protein Model Building By The Use Of A Constrained- Restrained Least-Squares Procedure' 99.23 129 98.45 98.45 5.95e-68 PDB 5LYZ 'Real-Space Refinement Of The Structure Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 6LYT 'Comparison Of Radiation-Induced Decay And Structure Refinement From X-Ray Data Collected From Lysozyme Crystals At Low And Ambient Temperatures' 99.23 129 98.45 98.45 5.95e-68 PDB 5LYM 'Studies Of Monoclinic Hen Egg White Lysozyme. Iv. X-Ray Refinement At 1.8 Angstrom Resolution And A Comparison Of The Variable Regions In The Polymorphic Forms' 99.23 129 98.45 98.45 5.95e-68 PDB 5LYT 'Comparison Of Radiation-Induced Decay And Structure Refinement From X-Ray Data Collected From Lysozyme Crystals At Low And Ambient Temperatures' 99.23 129 98.45 98.45 5.95e-68 PDB 4LYZ 'Real-Space Refinement Of The Structure Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 4LZT 'Atomic Resolution Refinement Of Triclinic Hew Lysozyme At 295k' 99.23 129 98.45 98.45 5.95e-68 PDB 4LYO 'Cross-Linked Chicken Lysozyme Crystal In Neat Acetonitrile, Then Back-Soaked In Water' 99.23 129 98.45 98.45 5.95e-68 PDB 4LYT 'Comparison Of Radiation-Induced Decay And Structure Refinement From X-Ray Data Collected From Lysozyme Crystals At Low And Ambient Temperatures' 99.23 129 98.45 98.45 5.95e-68 PDB 3LZT 'Refinement Of Triclinic Lysozyme At Atomic Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 4LYM 'Crystal Structure Of Low Humidity Tetragonal Lysozyme At 2.1-Angstroms Resolution. Variability In Hydration Shell And Its Structural Consequences' 99.23 129 98.45 98.45 5.95e-68 PDB 3LYT 'Comparison Of Radiation-Induced Decay And Structure Refinement From X-Ray Data Collected From Lysozyme Crystals At Low And Ambient Temperatures' 99.23 129 98.45 98.45 5.95e-68 PDB 3LYZ 'Real-Space Refinement Of The Structure Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 3LYM 'Crystal Structure Of Hen Egg-White Lysozyme At A Hydrostatic Pressure Of 1000 Atmospheres' 99.23 129 98.45 98.45 5.95e-68 PDB 3LYO 'Cross-Linked Chicken Lysozyme Crystal In 95% Acetonitrile- Water' 99.23 129 98.45 98.45 5.95e-68 PDB 3D9A 'High Resolution Crystal Structure Structure Of Hyhel10 Fab Complexed To Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 3HFM 'Structure Of An Antibody-Antigen Complex. Crystal Structure Of The HyHEL-10 Fab-Lysozyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 2Z19 'Phase Transition Of Monoclinic Lysozyme Crystal Soaked In A Saturated Nacl Solution' 99.23 129 98.45 98.45 5.95e-68 PDB 3B6L 'Crystal Structure Of Lysozyme Folded In Sds And 2-Methyl-2, 4-Pentanediol' 99.23 147 98.45 98.45 2.46e-68 PDB 2Z12 'Structure Of The Transformed Monoclinic Lysozyme By Controlled Dehydration' 99.23 129 98.45 98.45 5.95e-68 PDB 2Z18 'Phase Transition Of Monoclinic Lysozyme Crystal Soaked In A 10% Nacl Solution' 99.23 129 98.45 98.45 5.95e-68 PDB 2YSS 'Crystal Structure Of Humanized Hyhel-10 Fv Mutant(Hq39kw47y)-Hen Lysozyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 2YVB 'High Resolution X-Ray Crystal Structure Of Tetragonal Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2Q0M 'Tricarbonylmanganese(I)-Lysozyme Complex : A Structurally Characterized Organometallic Protein' 99.23 129 98.45 98.45 5.95e-68 PDB 2VB1 'Hewl At 0.65 Angstrom Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 2LZT 'Refinement Of Triclinic Lysozyme. Ii. The Method Of Stereochemically Restrained Least-Squares' 99.23 129 98.45 98.45 5.95e-68 PDB 2PC2 'Lysozyme Cocrystallized With Tris-Dipicolinate Eu Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 2LYZ 'Real-Space Refinement Of The Structure Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2LZH 'The Structures Of The Monoclinic And Orthorhombic Forms Of Hen Egg-White Lysozyme At 6 Angstroms Resolution.' 99.23 129 98.45 98.45 5.95e-68 PDB 2LYM 'Crystal Structure Of Hen Egg-White Lysozyme At A Hydrostatic Pressure Of 1000 Atmospheres' 99.23 129 98.45 98.45 5.95e-68 PDB 2LYO 'Cross-Linked Chicken Lysozyme Crystal In 90% Acetonitrile- Water' 99.23 129 98.45 98.45 5.95e-68 PDB 2I26 'Crystal Structure Analysis Of The Nurse Shark New Antigen Receptor Ancestral Variable Domain In Complex With Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2I6Z 'X-Ray Diffraction Studies Of Adducts Between Anticancer Platinum Drugs And Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2HUB 'Structure Of Hen Egg-White Lysozyme Determined From Crystals Grown In Ph 7.5' 99.23 129 98.45 98.45 5.95e-68 PDB 2I25 'Crystal Structure Analysis Of The Nurse Shark New Antigen Receptor Pbla8 Variable Domain In Complex With Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2HU1 'Crystal Structure Analysis Of Hen Egg White Lyszoyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2HU3 'Parent Structure Of Hen Egg White Lysozyme Grown In Acidic Ph 4.8. Refinement For Comparison With Crosslinked Molecules Of Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2HSO 'Multipattern Rietveld Refinement With Protein Powder Data: An Approach To Higher Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 2HTX 'Crystal Structure Analysis Of Hen Egg White Lysozyme Crosslinked By Polymerized Glutaraldehyde In Acidic Environment' 99.23 129 98.45 98.45 5.95e-68 PDB 2HS7 'Multipattern Rietveld Refinement With Protein Powder Data: An Approach To Higher Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 2HS9 'Multipattern Rietveld Refinement With Protein Powder Data: An Approach To Higher Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 2H9J 'Structure Of Hen Egg White Lysozyme Soaked With Ni2- Xylylbicyclam' 99.23 129 98.45 98.45 5.95e-68 PDB 2H9K 'Structure Of Hen Egg White Lysozyme Soaked With Ni-Cyclam' 99.23 129 98.45 98.45 5.95e-68 PDB 2G4P 'Anomalous Substructure Of Lysozyme At Ph 4.5' 99.23 129 98.45 98.45 5.95e-68 PDB 2G4Q 'Anomalous Substructure Of Lysozyme At Ph 8.0' 99.23 129 98.45 98.45 5.95e-68 PDB 2F4G 'Triclinic Cross-Linked Lysozyme Soaked In Bromoethanol 1m' 99.23 129 98.45 98.45 5.95e-68 PDB 2FBB 'Crystal Structure Analysis Of Hexagonal Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2F30 'Triclinic Cross-Linked Lysozyme Soaked With 4.5m Urea' 99.23 129 98.45 98.45 5.95e-68 PDB 2F4A 'Triclinic Cross-Linked Lysozyme Soaked With Thiourea 1.5m' 99.23 129 98.45 98.45 5.95e-68 PDB 2EPE 'Crystal Structure Analysis Of Hen Egg White Lysozyme Grown By Capillary Method' 99.23 129 98.45 98.45 5.95e-68 PDB 2F2N 'Triclinic Hen Egg Lysozyme Cross-Linked By Glutaraldehyde' 99.23 129 98.45 98.45 5.95e-68 PDB 2EIZ 'Crystal Structure Of Humanized Hyhel-10 Fv Mutant(Hw47y)- Hen Lysozyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 2EKS 'Crystal Structure Of Humanized Hyhel-10 Fv-Hen Lysozyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 2DQI 'Crystal Structure Of Hyhel-10 Fv Mutant (Ly50a) Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2DQJ 'Crystal Structure Of Hyhel-10 Fv (Wild-Type) Complexed With Hen Egg Lysozyme At 1.8a Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 2DQG 'Crystal Structure Of Hyhel-10 Fv Mutant (Hy53f) Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2DQH 'Crystal Structure Of Hyhel-10 Fv Mutant (Hy58a) Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2DQE 'Crystal Structure Of Hyhel-10 Fv Mutant (Hy53a) Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2DQF 'Crystal Structure Of Hyhel-10 Fv Mutant (Y33ay53a) Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2DQC 'Crystal Structure Of Hyhel-10 Fv Mutant(Hy33f) Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2DQD 'Crystal Structure Of Hyhel-10 Fv Mutant (Hy50f) Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2D6B 'Novel Bromate Species Trapped Within A Protein Crystal' 99.23 129 98.45 98.45 5.95e-68 PDB 2D91 'Structure Of Hyper-Vil-Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2D4J 'Transformed Monoclinic Crystal Of Hen Egg-White Lysozyme From A Heavy Water Solution' 99.23 129 98.45 98.45 5.95e-68 PDB 2D4K 'Monoclinic Hen Egg-White Lysozyme Crystallized At 313k' 99.23 129 98.45 98.45 5.95e-68 PDB 2CGI 'Siras Structure Of Tetragonal Lysosyme Using Derivative Data Collected At The High Energy Remote Holmium Kedge' 99.23 129 98.45 98.45 5.95e-68 PDB 2D4I 'Monoclinic Hen Egg-White Lysozyme Crystallized At Ph4.5 Form Heavy Water Solution' 99.23 129 98.45 98.45 5.95e-68 PDB 2C8P 'Lysozyme (60sec) And Uv Laser Excited Fluorescence' 99.23 129 98.45 98.45 5.95e-68 PDB 2CDS Lysozyme 99.23 129 98.45 98.45 5.95e-68 PDB 2BPU 'The Kedge Holmium Derivative Of Hen Egg-White Lysozyme At High Resolution From Single Wavelength Anomalous Diffraction' 99.23 129 98.45 98.45 5.95e-68 PDB 2C8O 'Lysozyme (1sec) And Uv Lasr Excited Fluorescence' 99.23 129 98.45 98.45 5.95e-68 PDB 2BLX 'Hewl Before A High Dose X-Ray "burn"' 99.23 129 98.45 98.45 5.95e-68 PDB 2BLY 'Hewl After A High Dose X-Ray "burn"' 99.23 129 98.45 98.45 5.95e-68 PDB 2AUB 'Lysozyme Structure Derived From Thin-Film-Based Crystals' 99.23 129 98.45 98.45 5.95e-68 PDB 2B5Z 'Hen Lysozyme Chemically Glycosylated' 99.23 129 98.45 98.45 5.95e-68 PDB 2A7D 'On The Routine Use Of Soft X-Rays In Macromolecular Crystallography, Part Iii- The Optimal Data Collection Wavelength' 99.23 129 98.45 98.45 5.95e-68 PDB 2A7F 'On The Routine Use Of Soft X-Rays In Macromolecular Crystallography, Part Iii- The Optimal Data Collection Wavelength' 99.23 129 98.45 98.45 5.95e-68 PDB 1ZVY 'Crystal Structure Of The Vhh D3-L11 In Complex With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 2A6U 'Ph Evolution Of Tetragonal Hewl At 4 Degrees Celcius.' 99.23 129 98.45 98.45 5.95e-68 PDB 1ZV5 'Crystal Structure Of The Variable Domain Of The Camelid Heavy-Chain Antibody D2-L29 In Complex With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1ZVH 'Crystal Stucture Of The Vhh Domain D2-L24 In Complex With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1Z55 'Effect Of Alcohols On Protein Hydration' 99.23 129 98.45 98.45 5.95e-68 PDB 1ZMY 'Cabbcii-10 Vhh Framework With Cdr Loops Of Cablys3 Grafted On It And In Complex With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1YL1 'Effect Of Alcohols On Protein Hydration' 99.23 129 98.45 98.45 5.95e-68 PDB 1YQV 'The Crystal Structure Of The Antibody Fab Hyhel5 Complex With Lysozyme At 1.7a Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 1YKZ 'Effect Of Alcohols On Protein Hydration' 99.23 129 98.45 98.45 5.95e-68 PDB 1YL0 'Effect Of Alcohols On Protein Hydration' 99.23 129 98.45 98.45 5.95e-68 PDB 1YKX 'Effect Of Alcohols On Protein Hydration' 99.23 129 98.45 98.45 5.95e-68 PDB 1YKY 'Effect Of Alcohols On Protein Hydration' 99.23 129 98.45 98.45 5.95e-68 PDB 1YIK 'Structure Of Hen Egg White Lysozyme Soaked With Cu-Cyclam' 99.23 129 98.45 98.45 5.95e-68 PDB 1YIL 'Structure Of Hen Egg White Lysozyme Soaked With Cu2- Xylylbicyclam' 99.23 129 98.45 98.45 5.95e-68 PDB 1XGT 'Structure For Antibody Hyhel-63 Y33l Mutant Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1XGU 'Structure For Antibody Hyhel-63 Y33f Mutant Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1XGQ 'Structure For Antibody Hyhel-63 Y33v Mutant Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1XGR 'Structure For Antibody Hyhel-63 Y33i Mutant Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1XFP 'Crystal Structure Of The Cdr2 Germline Reversion Mutant Of Cab-Lys3 In Complex With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1XGP 'Structure For Antibody Hyhel-63 Y33a Mutant Complexed With Hen Egg Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1XEJ 'The Crystal Structures Of Lysozyme At Very Low Levels Of Hydration' 99.23 129 98.45 98.45 5.95e-68 PDB 1XEK 'The Crystal Structures Of Lysozyme At Very Low Levels Of Hydration' 99.23 129 98.45 98.45 5.95e-68 PDB 1WTN 'The Structure Of Hew Lysozyme Orthorhombic Crystal Growth Under A High Magnetic Field' 99.23 129 98.45 98.45 5.95e-68 PDB 1XEI 'The Crystal Structures Of Lysozyme At Very Low Levels Of Hydration' 99.23 129 98.45 98.45 5.95e-68 PDB 1W6Z 'High Energy Tetragonal Lysozyme X-Ray Structure' 99.23 129 98.45 98.45 5.95e-68 PDB 1WTM 'X-Ray Structure Of Hew Lysozyme Orthorhombic Crystal Formed In The Earth's Magnetic Field' 99.23 129 98.45 98.45 5.95e-68 PDB 1VED 'The Crystal Structure Of The Orthorhombic Form Of Hen Egg White Lysozyme At 1.9 Angstroms Resolution In Space' 99.23 129 98.45 98.45 5.95e-68 PDB 1VFB 'Bound Water Molecules And Conformational Stabilization Help Mediate An Antigen-Antibody Association' 99.23 129 98.45 98.45 5.95e-68 PDB 1VDS 'The Crystal Structure Of The Tetragonal Form Of Hen Egg White Lysozyme At 1.6 Angstroms Resolution In Space' 99.23 129 98.45 98.45 5.95e-68 PDB 1VDT 'The Crystal Structure Of The Tetragonal Form Of Hen Egg White Lysozyme At 1.7 Angstroms Resolution Under Basic Conditions In Space' 99.23 129 98.45 98.45 5.95e-68 PDB 1VDP 'The Crystal Structure Of The Monoclinic Form Of Hen Egg White Lysozyme At 1.7 Angstroms Resolution In Space' 99.23 129 98.45 98.45 5.95e-68 PDB 1VDQ 'The Crystal Structure Of The Orthorhombic Form Of Hen Egg White Lysozyme At 1.5 Angstroms Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 1VAT 'Iodine Derivative Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1VAU 'Xenon Derivative Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1V7S 'Triclinic Hen Lysozyme Crystallized At 313k From A D2o Solution' 98.46 129 98.44 98.44 2.03e-67 PDB 1V7T 'Triclinic Lysozyme With Low Solvent Content Obtained By Phase Transition' 99.23 129 98.45 98.45 5.95e-68 PDB 1UIH 'Analysis Of The Stabilization Of Hen Lysozyme With The Helix Dipole And Charged Side Chains' 99.23 129 98.45 98.45 5.95e-68 PDB 1UUZ 'Ivy:a New Family Of Protein' 99.23 129 98.45 98.45 5.95e-68 PDB 1UCO 'Hen Egg-White Lysozyme, Low Humidity Form' 99.23 129 98.45 98.45 5.95e-68 PDB 1UIG 'Analysis Of The Stabilization Of Hen Lysozyme With The Helix Dipole And Charged Side Chains' 99.23 129 98.45 98.45 5.95e-68 PDB 1UA6 'Crystal Structure Of Hyhel-10 Fv Mutant Sfsf Complexed With Hen Egg White Lysozyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 1UC0 'Crystal Structure Of Wild-Type Hen-Egg White Lysozyme Singly Labeled With 2',3'-Epoxypropyl Beta-Glycoside Of N- Acetyllactosamine' 99.23 129 98.45 98.45 5.95e-68 PDB 1T3P 'Half-Sandwich Arene Ruthenium(Ii)-Enzyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 1T6V 'Crystal Structure Analysis Of The Nurse Shark New Antigen Receptor (Nar) Variable Domain In Complex With Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1SFG 'Binding Of Hexa-N-Acetylchitohexaose: A Powder Diffraction Study' 99.23 129 98.45 98.45 5.95e-68 PDB 1SQ2 'Crystal Structure Analysis Of The Nurse Shark New Antigen Receptor (Nar) Variable Domain In Complex With Lyxozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1SF7 'Binding Of Tetra-N-Acetylchitotetraose To Hew Lysozyme: A Powder Diffraction Study' 99.23 129 98.45 98.45 5.95e-68 PDB 1SFB 'Binding Of Penta-N-Acetylchitopentaose To Hew Lysozyme: A Powder Diffraction Study' 99.23 129 98.45 98.45 5.95e-68 PDB 1SF4 'Binding Of N,N'-Diacetylchitobiose To Hew Lysozyme: A Powder Diffraction Study' 99.23 129 98.45 98.45 5.95e-68 PDB 1SF6 'Binding Of N,N',N"-Triacetylchitotriose To Hew Lysozyme: A Powder Diffraction Study' 99.23 129 98.45 98.45 5.95e-68 PDB 1RI8 'Crystal Structure Of The Camelid Single Domain Antibody 1d2l19 In Complex With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1RJC 'Crystal Structure Of The Camelid Single Domain Antibody Cab- Lys2 In Complex With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1RCM 'Crystal Structure Of A Ubiquitin-Dependent Degradation Substrate: A Three-Disulfide Form Of Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1RFP 'Analysis Of The Stabilization Of Hen Lysozyme With The Helix Dipole And Charged Side Chains' 99.23 129 98.45 98.45 5.95e-68 PDB 1QIO 'Specific Chemical And Structural Damage Caused By Intense Synchrotron Radiation To Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1QTK 'Crystal Structure Of Hew Lysozyme Under Pressure Of Krypton (55 Bar)' 99.23 129 98.45 98.45 5.95e-68 PDB 1P2C 'Crystal Structure Analysis Of An Anti-Lysozyme Antibody' 99.23 129 98.45 98.45 5.95e-68 PDB 1PS5 'Structure Of The Monoclinic C2 Form Of Hen Egg-White Lysozyme At 2.0 Angstroms Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 1N4F 'Para-Arsanilate Derivative Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1NDM 'Crystal Structure Of Fab Fragment Of Antibody Hyhel-26 Complexed With Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1MEL 'Crystal Structure Of A Camel Single-Domain Vh Antibody Fragment In Complex With Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1MLC 'Monoclonal Antibody Fab D44.1 Raised Against Chicken Egg- White Lysozyme Complexed With Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1LZN 'Neutron Structure Of Hen Egg-White Lysozyme' 98.46 129 98.44 98.44 2.13e-67 PDB 1LZT 'Refinement Of Triclinic Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1LZC 'Dissection Of Protein-Carbohydrate Interactions In Mutant Hen Egg-White Lysozyme Complexes And Their Hydrolytic Activity' 99.23 129 98.45 98.45 5.95e-68 PDB 1LZH 'The Structures Of The Monoclinic And Orthorhombic Forms Of Hen Egg-White Lysozyme At 6 Angstroms Resolution.' 99.23 129 98.45 98.45 5.95e-68 PDB 1LZA 'Dissection Of Protein-Carbohydrate Interactions In Mutant Hen Egg-White Lysozyme Complexes And Their Hydrolytic Activity' 99.23 129 98.45 98.45 5.95e-68 PDB 1LZB 'Dissection Of Protein-Carbohydrate Interactions In Mutant Hen Egg-White Lysozyme Complexes And Their Hydrolytic Activity' 99.23 129 98.45 98.45 5.95e-68 PDB 1LZ8 'Lysozyme Phased On Anomalous Signal Of Sulfurs And Chlorines' 99.23 129 98.45 98.45 5.95e-68 PDB 1LZ9 'Anomalous Signal Of Solvent Bromines Used For Phasing Of Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1LYS 'X-Ray Structure Of A Monoclinic Form Of Hen Egg-White Lysozyme Crystallized At 313k. Comparison Of Two Independent Molecules' 99.23 129 98.45 98.45 5.95e-68 PDB 1LYZ 'Real-Space Refinement Of The Structure Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1LSG 'Three-Dimensional Structure Of The Platelet Integrin Recognition Segment Of The Fibrinogen Gamma Chain Obtained By Carrier Protein-Driven Crystallization' 100.00 144 98.46 98.46 4.48e-69 PDB 1LYO 'Cross-Linked Lysozyme Crystal In Neat Water' 99.23 129 98.45 98.45 5.95e-68 PDB 1LSE 'The Influence Of Temperature On Lysozyme Crystals. Structure And Dynamics Of Protein And Water' 99.23 129 98.45 98.45 5.95e-68 PDB 1LSF 'The Influence Of Temperature On Lysozyme Crystals. Structure And Dynamics Of Protein And Water' 99.23 129 98.45 98.45 5.95e-68 PDB 1LSC 'The Influence Of Temperature On Lysozyme Crystals. Structure And Dynamics Of Protein And Water' 99.23 129 98.45 98.45 5.95e-68 PDB 1LSD 'The Influence Of Temperature On Lysozyme Crystals. Structure And Dynamics Of Protein And Water' 99.23 129 98.45 98.45 5.95e-68 PDB 1LSA 'The Influence Of Temperature On Lysozyme Crystals. Structure And Dynamics Of Protein And Water' 99.23 129 98.45 98.45 5.95e-68 PDB 1LSB 'The Influence Of Temperature On Lysozyme Crystals. Structure And Dynamics Of Protein And Water' 99.23 129 98.45 98.45 5.95e-68 PDB 1LMA 'Protein Hydration And Water Structure: X-Ray Analysis Of A Closely Packed Protein Crystal With Very Low Solvent Content' 99.23 129 98.45 98.45 5.95e-68 PDB 1LPI 'Hew Lysozyme: Trp...Na Cation-Pi Interaction' 99.23 129 98.45 98.45 5.95e-68 PDB 1LKR 'Monoclinic Hen Egg White Lysozyme Iodide' 99.23 129 98.45 98.45 5.95e-68 PDB 1LKS 'Hen Egg White Lysozyme Nitrate' 99.23 129 98.45 98.45 5.95e-68 PDB 1LJJ 'Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 10% Trehalose' 99.23 129 98.45 98.45 5.95e-68 PDB 1LJK 'Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 15% Trehalose' 99.23 129 98.45 98.45 5.95e-68 PDB 1LJH 'Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 5% Glycerol' 99.23 129 98.45 98.45 5.95e-68 PDB 1LJI 'Crystal Structure Of Monoclinic Lysozyme Grown In Presence 10% Sorbitol' 99.23 129 98.45 98.45 5.95e-68 PDB 1LJF 'Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 10% Sucrose' 99.23 129 98.45 98.45 5.95e-68 PDB 1LJG 'Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 5% Glycerol' 99.23 129 98.45 98.45 5.95e-68 PDB 1LJ4 'Crystal Structure Of Monoclinic Lysozyme Grown At Ph 4.6' 99.23 129 98.45 98.45 5.95e-68 PDB 1LJE 'Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 10% Sucrose' 99.23 129 98.45 98.45 5.95e-68 PDB 1LCN 'Monoclinic Hen Egg White Lysozyme, Thiocyanate Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 1LJ3 'Crystal Structure Of Monoclinic Lysozyme Grown At Ph 4.6' 99.23 129 98.45 98.45 5.95e-68 PDB 1KIQ 'Fv Mutant Y(B 101)f (Vh Domain) Of Mouse Monoclonal Antibody D1.3 Complexed With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1KIR 'Fv Mutant Y(A 50)s (Vl Domain) Of Mouse Monoclonal Antibody D1.3 Complexed With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1JTT 'Degenerate Interfaces In Antigen-Antibody Complexes' 99.23 129 98.45 98.45 5.95e-68 PDB 1KIP 'Fv Mutant Y(B 32)a (Vh Domain) Of Mouse Monoclonal Antibody D1.3 Complexed With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1JPO 'Low Temperature Orthorhombic Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1JTO 'Degenerate Interfaces In Antigen-Antibody Complexes' 99.23 129 98.45 98.45 5.95e-68 PDB 1JJ1 'Crystal Structure Of Orthorhombic Lysozyme Grown At Ph 4.6 In Presence Of 5% Sorbitol' 99.23 129 98.45 98.45 5.95e-68 PDB 1JJ3 'Crystal Structure Of Monoclinic Lysozyme Grown At Ph 4.6' 99.23 129 98.45 98.45 5.95e-68 PDB 1JIY 'Crystal Structure Of Tetragonal Lysozyme Grown In Presence 20% Sorbitol' 99.23 129 98.45 98.45 5.95e-68 PDB 1JJ0 'Crystal Structure Of Tetragonal Lysozyme Grown In Presence Of 30% Sucrose' 99.23 129 98.45 98.45 5.95e-68 PDB 1JIS 'Crystal Structure Of Tetragonal Lysozyme Grown At Ph 4.6' 99.23 129 98.45 98.45 5.95e-68 PDB 1JIT 'Crystal Structure Of Tetragonal Lysozyme Grown In Presence 30% Trehalose' 99.23 129 98.45 98.45 5.95e-68 PDB 1JA6 'Binding Of N-Acetylglucosamine To Chicken Egg Lysozyme: A Powder Diffraction Study' 99.23 129 98.45 98.45 5.95e-68 PDB 1JA7 'Binding Of N-Acetylglucosamine To Chicken Egg Lysozyme: A Powder Diffraction Study' 99.23 129 98.45 98.45 5.95e-68 PDB 1JA2 'Binding Of N-Acetylglucosamine To Chicken Egg Lysozyme: A Powder Diffraction Study' 99.23 129 98.45 98.45 5.95e-68 PDB 1JA4 'Binding Of N-Acetylglucosamine To Chicken Egg Lysozyme: A Powder Diffraction Study' 99.23 129 98.45 98.45 5.95e-68 PDB 1J1P 'Crystal Structure Of Hyhel-10 Fv Mutant Ls91a Complexed With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1J1X 'Crystal Structure Of Hyhel-10 Fv Mutant Ls93a Complexed With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1IO5 'Hydrogen And Hydration Of Hen Egg-White Lysozyme Determined By Neutron Diffraction' 98.46 129 98.44 98.44 2.13e-67 PDB 1J1O 'Crystal Structure Of Hyhel-10 Fv Mutant Ly50f Complexed With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1IC7 'Crystal Structure Of Hyhel-10 Fv Mutant(Hd32a99a)-Hen Lysozyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 1IEE 'Structure Of Tetragonal Hen Egg White Lysozyme At 0.94 A From Crystals Grown By The Counter-Diffusion Method' 99.23 129 98.45 98.45 5.95e-68 PDB 1IC4 'Crystal Structure Of Hyhel-10 Fv Mutant(Hd32a)-Hen Lysozyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 1IC5 'Crystal Structure Of Hyhel-10 Fv Mutant(Hd99a)-Hen Lysozyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 1HSW 'Lysozyme (Mucopeptide N-Acetylmuramyl Hydrolase)' 99.23 129 98.45 98.45 5.95e-68 PDB 1HSX 'Lysozyme Grown At Basic Ph And Its Low Humidity Variant' 99.23 129 98.45 98.45 5.95e-68 PDB 1HEW 'Refinement Of An Enzyme Complex With Inhibitor Bound At Partial Occupancy. Hen Egg-White Lysozyme And Tri-N- Acetylchitotriose At 1.75 Angstroms Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 1HF4 'Structural Effects Of Monovalent Anions On Polymorphic Lysozyme Crystals' 99.23 129 98.45 98.45 5.95e-68 PDB 1HC0 'Structure Of Lysozyme With Periodate' 99.23 129 98.45 98.45 5.95e-68 PDB 1HEL 'Structural And Thermodynamic Analysis Of Compensating Mutations Within The Core Of Chicken Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1GXX 'Solution Structure Of Lysozyme At Low And High Pressure' 99.23 129 98.45 98.45 5.95e-68 PDB 1H87 'Gadolinium Derivative Of Tetragonal Hen Egg-White Lysozyme At 1.7 A Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 1GWD 'Tri-Iodide Derivative Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1GXV 'Solution Structure Of Lysozyme At Low And High Pressure' 99.23 129 98.45 98.45 5.95e-68 PDB 1G7M 'Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3 (Vlw92v)' 99.23 129 98.45 98.45 5.95e-68 PDB 1GPQ 'Structure Of Ivy Complexed With Its Target, Hewl' 99.23 129 98.45 98.45 5.95e-68 PDB 1G7J 'Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3 (Vlw92h)' 99.23 129 98.45 98.45 5.95e-68 PDB 1G7L 'Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3 (Vlw92s)' 99.23 129 98.45 98.45 5.95e-68 PDB 1G7H 'Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3(Vlw92a)' 99.23 129 98.45 98.45 5.95e-68 PDB 1G7I 'Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3 (Vlw92f)' 99.23 129 98.45 98.45 5.95e-68 PDB 1F10 'Crystal Structure Of Orthorhombic Lysozyme Grown At Ph 6.5 At 88% Relative Humidity' 99.23 129 98.45 98.45 5.95e-68 PDB 1FDL 'Crystallographic Refinement Of The Three-Dimensional Structure Of The Fab D1.3-Lysozyme Complex At 2.5- Angstroms Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 1E8L 'Nmr Solution Structure Of Hen Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1F0W 'Crystal Structure Of Orthorhombic Lysozyme Grown At Ph 6.5' 99.23 129 98.45 98.45 5.95e-68 PDB 1DPX 'Structure Of Hen Egg-White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1DQJ 'Crystal Structure Of The Anti-Lysozyme Antibody Hyhel-63 Complexed With Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1C10 'Crystal Structure Of Hew Lysozyme Under Pressure Of Xenon (8 Bar)' 99.23 129 98.45 98.45 5.95e-68 PDB 1DPW 'Structure Of Hen Egg-White Lysozyme In Complex With Mpd' 99.23 129 98.45 98.45 5.95e-68 PDB 1BWJ 'The 1.8 A Structure Of Microgravity Grown Tetragonal Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1C08 'Crystal Structure Of Hyhel-10 Fv-Hen Lysozyme Complex' 99.23 129 98.45 98.45 5.95e-68 PDB 1BWH 'The 1.8 A Structure Of Ground Control Grown Tetragonal Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1BWI 'The 1.8 A Structure Of Microbatch Oil Drop Grown Tetragonal Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1BVK 'Humanized Anti-Lysozyme Fv Complexed With Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1BVX 'The 1.8 A Structure Of Gel Grown Tetragonal Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 1BGI 'Orthorhombic Lysozyme Crystallized At High Temperature (310k)' 99.23 129 98.45 98.45 5.95e-68 PDB 1BHZ 'Low Temperature Middle Resolution Structure Of Hen Egg White Lysozyme From Masc Data' 99.23 129 98.45 98.45 5.95e-68 PDB 1B0D 'Structural Effects Of Monovalent Anions On Polymorphic Lysozyme Crystals' 99.23 129 98.45 98.45 5.95e-68 PDB 1B2K 'Structural Effects Of Monovalent Anions On Polymorphic Lysozyme Crystals' 99.23 129 98.45 98.45 5.95e-68 PDB 1AKI 'The Structure Of The Orthorhombic Form Of Hen Egg-White Lysozyme At 1.5 Angstroms Resolution' 99.23 129 98.45 98.45 5.95e-68 PDB 1AZF 'Chicken Egg White Lysozyme Crystal Grown In Bromide Solution' 99.23 129 98.45 98.45 5.95e-68 PDB 193L 'The 1.33 A Structure Of Tetragonal Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 PDB 194L 'The 1.40 A Structure Of Spacehab-01 Hen Egg White Lysozyme' 99.23 129 98.45 98.45 5.95e-68 BMRB 4943 'Chicken Egg White Lysozyme' 99.23 133 98.45 98.45 5.52e-68 BMRB 5068 'hen egg white lysozyme' 100.00 130 98.46 98.46 9.56e-69 BMRB 1093 lysozyme 99.23 129 98.45 98.45 5.95e-68 BMRB 4562 'hen egg white lysozyme' 99.23 129 98.45 98.45 5.95e-68 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Tissue $C30A_C115A chicken 9031 Eukaryota Metazoa Gallus gallus 'egg white' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $C30A_C115A 'recombinant technology' 'E. coli' Escherichia coli AD18 plasmid . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $C30A_C115A . mM 0.2 1.0 [U-15N] stop_ save_ ############################ # Computer software used # ############################ save_XWINNMR _Saveframe_category software _Name XWINNMR _Version 2.6 _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_DQF-COSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _Sample_label $sample_1 save_ save_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name NOESY _Sample_label $sample_1 save_ save_HOHAHA_3 _Saveframe_category NMR_applied_experiment _Experiment_name HOHAHA _Sample_label $sample_1 save_ save_15N_3D-NOESY-HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '15N 3D-NOESY-HSQC' _Sample_label $sample_1 save_ save_15N_3D-TOCSY-HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '15N 3D-TOCSY-HSQC' _Sample_label $sample_1 save_ save_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '1H-15N HSQC' _Sample_label $sample_1 save_ save_15N_3D-HSQC-NOESY-HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '15N 3D-HSQC-NOESY-HSQC' _Sample_label $sample_1 save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name NOESY _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name HOHAHA _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name '15N 3D-NOESY-HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_5 _Saveframe_category NMR_applied_experiment _Experiment_name '15N 3D-TOCSY-HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_6 _Saveframe_category NMR_applied_experiment _Experiment_name '1H-15N HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_7 _Saveframe_category NMR_applied_experiment _Experiment_name '15N 3D-HSQC-NOESY-HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_EX-cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 3.8 0.1 na temperature 298 0.1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.0 external direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label DQF-COSY NOESY HOHAHA '15N 3D-NOESY-HSQC' '15N 3D-TOCSY-HSQC' '1H-15N HSQC' '15N 3D-HSQC-NOESY-HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $EX-cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'hen lysozyme' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 2 LYS H H 8.80 0.02 1 2 1 2 LYS HA H 4.36 0.02 1 3 1 2 LYS HB2 H 1.76 0.02 2 4 1 2 LYS HB3 H 1.58 0.02 2 5 2 3 VAL H H 8.81 0.02 1 6 2 3 VAL HA H 4.83 0.02 1 7 2 3 VAL HB H 2.01 0.02 1 8 2 3 VAL HG1 H 1.04 0.02 2 9 2 3 VAL HG2 H 0.94 0.02 2 10 3 4 PHE H H 8.63 0.02 1 11 3 4 PHE HA H 4.26 0.02 1 12 3 4 PHE HB2 H 3.17 0.02 2 13 3 4 PHE HB3 H 2.63 0.02 2 14 3 4 PHE HD1 H 6.97 0.02 1 15 3 4 PHE HD2 H 6.97 0.02 1 16 3 4 PHE HE1 H 7.22 0.02 1 17 3 4 PHE HE2 H 7.22 0.02 1 18 3 4 PHE HZ H 7.48 0.02 1 19 4 5 GLY H H 8.45 0.02 1 20 4 5 GLY HA2 H 4.31 0.02 2 21 4 5 GLY HA3 H 3.94 0.02 2 22 5 6 ARG H H 8.59 0.02 1 23 5 6 ARG HA H 3.42 0.02 1 24 5 6 ARG HB2 H 2.08 0.02 2 25 5 6 ARG HG2 H 1.70 0.02 2 26 5 6 ARG HD2 H 3.78 0.02 2 27 5 6 ARG HD3 H 3.63 0.02 2 28 5 6 ARG HE H 7.55 0.02 1 29 6 7 CYS H H 8.67 0.02 1 30 6 7 CYS HA H 4.75 0.02 1 31 6 7 CYS HB2 H 3.23 0.02 2 32 6 7 CYS HB3 H 2.77 0.02 2 33 7 8 GLU H H 8.28 0.02 1 34 7 8 GLU HA H 4.10 0.02 1 35 7 8 GLU HB2 H 2.29 0.02 2 36 7 8 GLU HG2 H 2.34 0.02 2 37 7 8 GLU HG3 H 2.50 0.02 2 38 8 9 LEU H H 8.60 0.02 1 39 8 9 LEU HA H 3.76 0.02 1 40 8 9 LEU HB2 H 1.61 0.02 2 41 8 9 LEU HB3 H 0.94 0.02 2 42 8 9 LEU HG H 1.53 0.02 1 43 8 9 LEU HD1 H 0.58 0.02 2 44 8 9 LEU HD2 H -0.01 0.02 2 45 9 10 ALA H H 8.43 0.02 1 46 9 10 ALA HA H 3.62 0.02 1 47 9 10 ALA HB H 1.57 0.02 1 48 10 11 ALA H H 8.16 0.02 1 49 10 11 ALA HA H 3.98 0.02 1 50 10 11 ALA HB H 1.55 0.02 1 51 11 12 ALA H H 7.79 0.02 1 52 11 12 ALA HA H 4.26 0.02 1 53 11 12 ALA HB H 1.51 0.02 1 54 12 13 MET H H 9.16 0.02 1 55 12 13 MET HA H 3.46 0.02 1 56 12 13 MET HB2 H 1.91 0.02 2 57 12 13 MET HB3 H 1.55 0.02 2 58 12 13 MET HG2 H 1.36 0.02 2 59 12 13 MET HG3 H 2.75 0.02 2 60 12 13 MET HE H 1.69 0.02 1 61 13 14 LYS H H 8.55 0.02 1 62 13 14 LYS HA H 3.96 0.02 1 63 13 14 LYS HB2 H 2.21 0.02 2 64 13 14 LYS HD2 H 1.74 0.02 2 65 13 14 LYS HD3 H 3.03 0.02 2 66 14 15 ARG H H 8.20 0.02 1 67 14 15 ARG HA H 4.14 0.02 1 68 14 15 ARG HB2 H 1.96 0.02 2 69 14 15 ARG HD2 H 3.14 0.02 2 70 14 15 ARG HE H 7.16 0.02 1 71 15 16 HIS H H 7.36 0.02 1 72 15 16 HIS HA H 4.56 0.02 1 73 15 16 HIS HB2 H 3.70 0.02 2 74 15 16 HIS HB3 H 2.58 0.02 2 75 15 16 HIS HD2 H 7.51 0.02 1 76 15 16 HIS HE1 H 8.80 0.02 1 77 16 17 GLY H H 7.65 0.02 1 78 16 17 GLY HA2 H 4.12 0.02 2 79 16 17 GLY HA3 H 3.92 0.02 2 80 17 18 LEU H H 7.16 0.02 1 81 17 18 LEU HA H 3.94 0.02 1 82 17 18 LEU HB2 H 0.75 0.02 2 83 17 18 LEU HB3 H 0.35 0.02 2 84 17 18 LEU HG H 0.72 0.02 1 85 17 18 LEU HD1 H -0.68 0.02 2 86 17 18 LEU HD2 H -0.07 0.02 2 87 18 19 ASP H H 8.79 0.02 1 88 18 19 ASP HA H 4.28 0.02 1 89 18 19 ASP HB2 H 2.98 0.02 2 90 18 19 ASP HB3 H 2.41 0.02 2 91 19 20 ASN H H 8.37 0.02 1 92 19 20 ASN HA H 4.07 0.02 1 93 19 20 ASN HB2 H 2.83 0.02 2 94 19 20 ASN HB3 H 3.06 0.02 2 95 19 20 ASN HD21 H 6.66 0.02 2 96 19 20 ASN HD22 H 7.48 0.02 2 97 20 21 TYR H H 8.19 0.02 1 98 20 21 TYR HA H 4.28 0.02 1 99 20 21 TYR HB2 H 3.08 0.02 2 100 20 21 TYR HB3 H 3.22 0.02 2 101 20 21 TYR HD1 H 7.22 0.02 1 102 20 21 TYR HD2 H 7.22 0.02 1 103 20 21 TYR HE1 H 7.06 0.02 1 104 20 21 TYR HE2 H 7.06 0.02 1 105 21 22 ARG H H 9.01 0.02 1 106 21 22 ARG HA H 3.63 0.02 1 107 21 22 ARG HB2 H 1.87 0.02 2 108 21 22 ARG HB3 H 2.20 0.02 2 109 21 22 ARG HG2 H 1.30 0.02 2 110 21 22 ARG HG3 H 1.16 0.02 2 111 21 22 ARG HD2 H 3.06 0.02 2 112 21 22 ARG HE H 7.13 0.02 1 113 22 23 GLY H H 7.66 0.02 1 114 22 23 GLY HA2 H 3.96 0.02 2 115 22 23 GLY HA3 H 3.48 0.02 2 116 23 24 TYR H H 7.76 0.02 1 117 23 24 TYR HA H 4.58 0.02 1 118 23 24 TYR HB2 H 3.36 0.02 2 119 23 24 TYR HB3 H 2.47 0.02 2 120 23 24 TYR HD1 H 7.05 0.02 1 121 23 24 TYR HD2 H 7.05 0.02 1 122 23 24 TYR HE1 H 6.74 0.02 1 123 23 24 TYR HE2 H 6.74 0.02 1 124 24 25 SER H H 8.98 0.02 1 125 24 25 SER HA H 4.55 0.02 1 126 25 26 LEU H H 9.04 0.02 1 127 25 26 LEU HA H 4.39 0.02 1 128 25 26 LEU HB2 H 1.90 0.02 2 129 25 26 LEU HG H 1.67 0.02 1 130 25 26 LEU HD1 H 0.96 0.02 2 131 25 26 LEU HD2 H 1.04 0.02 2 132 26 27 GLY H H 9.44 0.02 1 133 26 27 GLY HA2 H 4.06 0.02 2 134 26 27 GLY HA3 H 3.67 0.02 2 135 27 28 ASN H H 7.92 0.02 1 136 27 28 ASN HA H 4.16 0.02 1 137 27 28 ASN HB2 H 2.75 0.02 2 138 27 28 ASN HB3 H 2.18 0.02 2 139 27 28 ASN HD21 H 7.43 0.02 2 140 27 28 ASN HD22 H 7.54 0.02 2 141 28 29 TRP H H 7.26 0.02 1 142 28 29 TRP HA H 3.81 0.02 1 143 28 29 TRP HB2 H 3.25 0.02 2 144 28 29 TRP HD1 H 7.27 0.02 1 145 28 29 TRP HE1 H 9.29 0.02 1 146 28 29 TRP HE3 H 6.86 0.02 1 147 28 29 TRP HZ2 H 7.49 0.02 1 148 28 29 TRP HZ3 H 6.31 0.02 1 149 28 29 TRP HH2 H 6.77 0.02 1 150 29 30 VAL H H 7.71 0.02 1 151 29 30 VAL HA H 3.40 0.02 1 152 29 30 VAL HB H 1.97 0.02 1 153 29 30 VAL HG1 H 1.20 0.02 2 154 29 30 VAL HG2 H 0.94 0.02 2 155 30 31 ALA H H 8.00 0.02 1 156 30 31 ALA HA H 2.49 0.02 1 157 30 31 ALA HB H 0.90 0.02 1 158 31 32 ALA H H 7.86 0.02 1 159 31 32 ALA HA H 4.26 0.02 1 160 31 32 ALA HB H 1.11 0.02 1 161 32 33 ALA H H 7.37 0.02 1 162 32 33 ALA HA H 4.11 0.02 1 163 32 33 ALA HB H 1.33 0.02 1 164 33 34 LYS H H 7.93 0.02 1 165 33 34 LYS HA H 2.78 0.02 1 166 33 34 LYS HE2 H 2.62 0.02 2 167 33 34 LYS HE3 H 2.80 0.02 2 168 33 34 LYS HZ H 7.42 0.02 1 169 34 35 PHE H H 7.38 0.02 1 170 34 35 PHE HE1 H 7.46 0.02 1 171 34 35 PHE HE2 H 7.46 0.02 1 172 34 35 PHE HZ H 7.55 0.02 1 173 35 36 GLU H H 8.48 0.02 1 174 35 36 GLU HA H 4.30 0.02 1 175 35 36 GLU HB2 H 2.06 0.02 2 176 35 36 GLU HB3 H 1.87 0.02 2 177 36 37 SER H H 8.10 0.02 1 178 36 37 SER HA H 4.55 0.02 1 179 36 37 SER HB2 H 3.77 0.02 2 180 36 37 SER HB3 H 4.59 0.02 2 181 37 38 ASN H H 8.34 0.02 1 182 37 38 ASN HA H 4.44 0.02 1 183 37 38 ASN HB2 H 3.31 0.02 2 184 37 38 ASN HB3 H 2.50 0.02 2 185 37 38 ASN HD21 H 6.77 0.02 2 186 37 38 ASN HD22 H 7.43 0.02 2 187 38 39 PHE H H 7.47 0.02 1 188 38 39 PHE HA H 3.91 0.02 1 189 38 39 PHE HB2 H 3.82 0.02 2 190 38 39 PHE HD1 H 6.96 0.02 1 191 38 39 PHE HD2 H 6.96 0.02 1 192 38 39 PHE HE1 H 7.41 0.02 1 193 38 39 PHE HE2 H 7.41 0.02 1 194 38 39 PHE HZ H 6.93 0.02 1 195 39 40 ASN H H 7.64 0.02 1 196 39 40 ASN HA H 4.96 0.02 1 197 39 40 ASN HB2 H 3.00 0.02 2 198 39 40 ASN HB3 H 3.44 0.02 2 199 39 40 ASN HD21 H 7.03 0.02 2 200 39 40 ASN HD22 H 7.62 0.02 2 201 41 42 GLN H H 8.06 0.02 1 202 41 42 GLN HA H 4.51 0.02 1 203 41 42 GLN HB2 H 2.46 0.02 2 204 41 42 GLN HB3 H 1.90 0.02 2 205 41 42 GLN HE21 H 6.82 0.02 2 206 41 42 GLN HE22 H 7.66 0.02 2 207 42 43 ALA H H 6.85 0.02 1 208 42 43 ALA HA H 4.12 0.02 1 209 42 43 ALA HB H 1.34 0.02 1 210 43 44 THR H H 8.34 0.02 1 211 43 44 THR HA H 5.11 0.02 1 212 43 44 THR HB H 3.73 0.02 1 213 43 44 THR HG2 H 1.05 0.02 1 214 44 45 ASN H H 8.16 0.02 1 215 44 45 ASN HA H 5.01 0.02 1 216 44 45 ASN HB2 H 2.74 0.02 2 217 44 45 ASN HB3 H 2.69 0.02 2 218 44 45 ASN HD21 H 6.92 0.02 2 219 44 45 ASN HD22 H 7.39 0.02 2 220 45 46 ARG H H 8.93 0.02 1 221 45 46 ARG HA H 4.49 0.02 1 222 45 46 ARG HB2 H 1.82 0.02 2 223 45 46 ARG HB3 H 1.70 0.02 2 224 45 46 ARG HG2 H 1.63 0.02 2 225 45 46 ARG HD2 H 3.13 0.02 2 226 45 46 ARG HE H 7.10 0.02 1 227 46 47 ASN H H 8.96 0.02 1 228 46 47 ASN HA H 5.11 0.02 1 229 46 47 ASN HB2 H 2.83 0.02 2 230 46 47 ASN HD21 H 6.85 0.02 2 231 46 47 ASN HD22 H 6.99 0.02 2 232 47 48 THR H H 8.88 0.02 1 233 47 48 THR HA H 4.10 0.02 1 234 47 48 THR HB H 4.37 0.02 1 235 47 48 THR HG2 H 1.34 0.02 1 236 48 49 ASP H H 7.87 0.02 1 237 48 49 ASP HA H 4.57 0.02 1 238 48 49 ASP HB2 H 3.10 0.02 2 239 48 49 ASP HB3 H 2.67 0.02 2 240 49 50 GLY H H 7.91 0.02 1 241 49 50 GLY HA2 H 4.42 0.02 2 242 49 50 GLY HA3 H 3.75 0.02 2 243 50 51 SER H H 8.30 0.02 1 244 50 51 SER HA H 4.58 0.02 1 245 50 51 SER HB2 H 3.86 0.02 2 246 51 52 THR H H 9.22 0.02 1 247 51 52 THR HA H 4.90 0.02 1 248 51 52 THR HB H 3.77 0.02 1 249 51 52 THR HG2 H 0.29 0.02 1 250 52 53 ASP H H 8.78 0.02 1 251 52 53 ASP HA H 5.20 0.02 1 252 52 53 ASP HB2 H 2.60 0.02 2 253 52 53 ASP HB3 H 1.98 0.02 2 254 53 54 TYR H H 9.03 0.02 1 255 53 54 TYR HA H 4.76 0.02 1 256 53 54 TYR HB2 H 2.96 0.02 2 257 53 54 TYR HB3 H 2.77 0.02 2 258 53 54 TYR HD1 H 6.83 0.02 1 259 53 54 TYR HD2 H 6.83 0.02 1 260 53 54 TYR HE1 H 6.57 0.02 1 261 53 54 TYR HE2 H 6.57 0.02 1 262 54 55 GLY H H 9.12 0.02 1 263 54 55 GLY HA2 H 4.46 0.02 2 264 54 55 GLY HA3 H 4.06 0.02 2 265 55 56 ILE H H 9.44 0.02 1 266 55 56 ILE HB H 1.67 0.02 1 267 55 56 ILE HG12 H 1.12 0.02 2 268 55 56 ILE HG13 H 1.49 0.02 2 269 55 56 ILE HG2 H 0.93 0.02 1 270 55 56 ILE HD1 H 0.76 0.02 1 271 56 57 LEU H H 8.95 0.02 1 272 56 57 LEU HA H 4.45 0.02 1 273 56 57 LEU HB2 H 1.54 0.02 2 274 56 57 LEU HG H 1.23 0.02 1 275 56 57 LEU HD1 H 0.40 0.02 2 276 56 57 LEU HD2 H 0.55 0.02 2 277 57 58 GLN H H 7.95 0.02 1 278 57 58 GLN HA H 3.38 0.02 1 279 57 58 GLN HB2 H 2.20 0.02 2 280 57 58 GLN HB3 H 2.25 0.02 2 281 57 58 GLN HE21 H 8.29 0.02 2 282 57 58 GLN HE22 H 5.24 0.02 2 283 58 59 ILE H H 7.65 0.02 1 284 58 59 ILE HA H 3.99 0.02 1 285 58 59 ILE HB H 1.82 0.02 1 286 58 59 ILE HG12 H 1.82 0.02 2 287 58 59 ILE HG13 H 1.46 0.02 2 288 58 59 ILE HG2 H 1.04 0.02 1 289 58 59 ILE HD1 H 0.87 0.02 1 290 59 60 ASN H H 8.60 0.02 1 291 59 60 ASN HA H 5.64 0.02 1 292 59 60 ASN HB2 H 3.45 0.02 2 293 59 60 ASN HB3 H 3.04 0.02 2 294 60 61 SER H H 9.18 0.02 1 295 60 61 SER HA H 5.17 0.02 1 296 60 61 SER HB2 H 4.42 0.02 2 297 61 62 ARG H H 8.78 0.02 1 298 61 62 ARG HA H 4.10 0.02 1 299 61 62 ARG HB2 H 1.75 0.02 2 300 61 62 ARG HB3 H 1.48 0.02 2 301 61 62 ARG HG2 H 0.66 0.02 2 302 61 62 ARG HG3 H 1.01 0.02 2 303 61 62 ARG HD2 H 2.86 0.02 2 304 61 62 ARG HE H 6.78 0.02 1 305 62 63 TRP H H 7.20 0.02 1 306 62 63 TRP HA H 4.37 0.02 1 307 62 63 TRP HB2 H 1.84 0.02 2 308 62 63 TRP HD1 H 7.10 0.02 1 309 62 63 TRP HE1 H 10.22 0.02 1 310 62 63 TRP HE3 H 7.19 0.02 1 311 62 63 TRP HZ2 H 7.44 0.02 1 312 62 63 TRP HZ3 H 7.03 0.02 1 313 62 63 TRP HH2 H 7.19 0.02 1 314 63 64 TRP H H 7.41 0.02 1 315 63 64 TRP HA H 4.98 0.02 1 316 63 64 TRP HB2 H 3.42 0.02 2 317 63 64 TRP HD1 H 7.66 0.02 1 318 63 64 TRP HE1 H 10.25 0.02 1 319 63 64 TRP HE3 H 7.76 0.02 1 320 63 64 TRP HZ2 H 7.20 0.02 1 321 63 64 TRP HZ3 H 6.83 0.02 1 322 63 64 TRP HH2 H 7.10 0.02 1 323 64 65 CYS H H 7.58 0.02 1 324 64 65 CYS HA H 5.83 0.02 1 325 64 65 CYS HB2 H 3.16 0.02 2 326 64 65 CYS HB3 H 2.49 0.02 2 327 65 66 ASN H H 8.36 0.02 1 328 65 66 ASN HA H 5.55 0.02 1 329 65 66 ASN HB2 H 2.83 0.02 2 330 65 66 ASN HB3 H 2.46 0.02 2 331 65 66 ASN HD21 H 7.08 0.02 2 332 65 66 ASN HD22 H 7.64 0.02 2 333 66 67 ASP H H 9.84 0.02 1 334 66 67 ASP HA H 4.98 0.02 1 335 66 67 ASP HB2 H 3.28 0.02 2 336 66 67 ASP HB3 H 2.23 0.02 2 337 67 68 GLY H H 8.33 0.02 1 338 67 68 GLY HA2 H 4.14 0.02 2 339 67 68 GLY HA3 H 3.87 0.02 2 340 68 69 ARG H H 8.09 0.02 1 341 68 69 ARG HA H 4.78 0.02 1 342 68 69 ARG HB2 H 1.83 0.02 2 343 68 69 ARG HG2 H 1.63 0.02 2 344 68 69 ARG HG3 H 1.70 0.02 2 345 68 69 ARG HD2 H 3.18 0.02 2 346 68 69 ARG HD3 H 3.11 0.02 2 347 68 69 ARG HE H 7.06 0.02 1 348 69 70 THR H H 8.27 0.02 1 349 69 70 THR HA H 4.60 0.02 1 350 69 70 THR HB H 4.19 0.02 1 351 69 70 THR HG2 H 0.90 0.02 1 352 70 71 PRO HA H 4.43 0.02 1 353 70 71 PRO HB2 H 1.87 0.02 2 354 70 71 PRO HB3 H 2.29 0.02 2 355 70 71 PRO HG2 H 1.97 0.02 2 356 70 71 PRO HD2 H 4.05 0.02 2 357 70 71 PRO HD3 H 3.62 0.02 2 358 71 72 GLY H H 8.77 0.02 1 359 71 72 GLY HA2 H 3.81 0.02 2 360 71 72 GLY HA3 H 3.66 0.02 2 361 72 73 SER H H 7.31 0.02 1 362 72 73 SER HA H 4.58 0.02 1 363 72 73 SER HB2 H 4.25 0.02 2 364 72 73 SER HB3 H 3.78 0.02 2 365 73 74 ARG H H 8.04 0.02 1 366 73 74 ARG HA H 4.25 0.02 1 367 73 74 ARG HB2 H 1.83 0.02 2 368 73 74 ARG HG2 H 1.51 0.02 2 369 73 74 ARG HG3 H 1.67 0.02 2 370 73 74 ARG HD2 H 2.87 0.02 2 371 73 74 ARG HE H 7.03 0.02 1 372 74 75 ASN H H 8.37 0.02 1 373 74 75 ASN HA H 3.76 0.02 1 374 74 75 ASN HB2 H 3.06 0.02 2 375 74 75 ASN HB3 H 2.01 0.02 2 376 74 75 ASN HD21 H 7.33 0.02 2 377 74 75 ASN HD22 H 6.25 0.02 2 378 75 76 LEU H H 9.10 0.02 1 379 75 76 LEU HA H 4.09 0.02 1 380 75 76 LEU HB2 H 2.12 0.02 2 381 75 76 LEU HB3 H 1.48 0.02 2 382 75 76 LEU HG H 1.65 0.02 1 383 75 76 LEU HD1 H 0.51 0.02 2 384 75 76 LEU HD2 H 0.86 0.02 2 385 76 77 CYS H H 9.52 0.02 1 386 76 77 CYS HA H 4.44 0.02 1 387 76 77 CYS HB2 H 3.64 0.02 2 388 77 78 ASN H H 8.04 0.02 1 389 77 78 ASN HA H 4.21 0.02 1 390 77 78 ASN HB2 H 3.16 0.02 2 391 77 78 ASN HB3 H 2.50 0.02 2 392 77 78 ASN HD21 H 6.80 0.02 2 393 77 78 ASN HD22 H 7.46 0.02 2 394 78 79 ILE H H 8.84 0.02 1 395 78 79 ILE HA H 4.98 0.02 1 396 78 79 ILE HB H 1.68 0.02 1 397 78 79 ILE HG12 H 1.05 0.02 2 398 78 79 ILE HG13 H 1.46 0.02 2 399 78 79 ILE HG2 H 0.86 0.02 1 400 78 79 ILE HD1 H 0.87 0.02 1 401 79 80 PRO HA H 5.16 0.02 1 402 79 80 PRO HB2 H 2.35 0.02 2 403 79 80 PRO HB3 H 2.02 0.02 2 404 79 80 PRO HG2 H 2.10 0.02 2 405 79 80 PRO HG3 H 2.03 0.02 2 406 79 80 PRO HD2 H 3.66 0.02 2 407 79 80 PRO HD3 H 3.83 0.02 2 408 80 81 CYS H H 8.27 0.02 1 409 80 81 CYS HA H 3.87 0.02 1 410 80 81 CYS HB2 H 1.72 0.02 2 411 80 81 CYS HB3 H 1.26 0.02 2 412 81 82 SER H H 8.61 0.02 1 413 81 82 SER HA H 3.80 0.02 1 414 82 83 ALA H H 7.62 0.02 1 415 82 83 ALA HA H 4.23 0.02 1 416 82 83 ALA HB H 1.49 0.02 1 417 83 84 LEU H H 7.85 0.02 1 418 83 84 LEU HA H 4.26 0.02 1 419 83 84 LEU HB2 H 2.05 0.02 2 420 83 84 LEU HG H 1.63 0.02 1 421 83 84 LEU HD1 H 0.71 0.02 2 422 83 84 LEU HD2 H 1.01 0.02 2 423 84 85 LEU H H 7.11 0.02 1 424 84 85 LEU HA H 4.97 0.02 1 425 84 85 LEU HB2 H 1.92 0.02 2 426 84 85 LEU HB3 H 1.72 0.02 2 427 84 85 LEU HG H 1.60 0.02 1 428 84 85 LEU HD1 H 0.99 0.02 2 429 84 85 LEU HD2 H 0.88 0.02 2 430 85 86 SER H H 6.89 0.02 1 431 85 86 SER HA H 4.53 0.02 1 432 85 86 SER HB2 H 4.12 0.02 2 433 85 86 SER HB3 H 3.81 0.02 2 434 86 87 SER H H 8.48 0.02 1 435 86 87 SER HA H 4.23 0.02 1 436 86 87 SER HB2 H 3.91 0.02 2 437 87 88 ASP H H 8.24 0.02 1 438 87 88 ASP HA H 4.92 0.02 1 439 87 88 ASP HB2 H 2.95 0.02 2 440 87 88 ASP HB3 H 2.63 0.02 2 441 88 89 ILE H H 8.09 0.02 1 442 88 89 ILE HA H 4.68 0.02 1 443 88 89 ILE HB H 1.83 0.02 1 444 88 89 ILE HG12 H 0.35 0.02 2 445 88 89 ILE HG13 H 1.15 0.02 2 446 88 89 ILE HG2 H 0.77 0.02 1 447 88 89 ILE HD1 H 0.21 0.02 1 448 89 90 THR H H 8.33 0.02 1 449 89 90 THR HA H 3.05 0.02 1 450 89 90 THR HB H 4.05 0.02 1 451 89 90 THR HG2 H 1.15 0.02 1 452 90 91 ALA H H 9.18 0.02 1 453 90 91 ALA HA H 4.06 0.02 1 454 90 91 ALA HB H 1.33 0.02 1 455 91 92 SER H H 7.73 0.02 1 456 91 92 SER HA H 4.08 0.02 1 457 91 92 SER HB2 H 3.48 0.02 2 458 91 92 SER HB3 H 3.89 0.02 2 459 91 92 SER HG H 5.97 0.02 1 460 92 93 VAL H H 8.39 0.02 1 461 92 93 VAL HA H 3.11 0.02 1 462 92 93 VAL HB H 1.88 0.02 1 463 92 93 VAL HG1 H 0.60 0.02 2 464 92 93 VAL HG2 H 0.47 0.02 2 465 93 94 ASN H H 8.66 0.02 1 466 93 94 ASN HA H 4.26 0.02 1 467 93 94 ASN HB2 H 2.74 0.02 2 468 93 94 ASN HB3 H 2.88 0.02 2 469 93 94 ASN HD21 H 7.67 0.02 2 470 93 94 ASN HD22 H 6.98 0.02 2 471 94 95 CYS H H 7.83 0.02 1 472 94 95 CYS HA H 4.97 0.02 1 473 94 95 CYS HB2 H 3.34 0.02 2 474 94 95 CYS HB3 H 2.67 0.02 2 475 95 96 ALA H H 8.70 0.02 1 476 95 96 ALA HA H 3.97 0.02 1 477 95 96 ALA HB H 1.47 0.02 1 478 96 97 LYS H H 8.02 0.02 1 479 96 97 LYS HA H 3.71 0.02 1 480 96 97 LYS HB2 H 1.12 0.02 2 481 96 97 LYS HB3 H 1.66 0.02 2 482 96 97 LYS HG2 H -0.40 0.02 2 483 96 97 LYS HD2 H 1.26 0.02 2 484 96 97 LYS HE2 H 2.05 0.02 2 485 96 97 LYS HE3 H 2.15 0.02 2 486 97 98 LYS H H 7.22 0.02 1 487 97 98 LYS HA H 4.13 0.02 1 488 97 98 LYS HB2 H 2.14 0.02 2 489 98 99 ILE H H 7.97 0.02 1 490 98 99 ILE HA H 2.80 0.02 1 491 98 99 ILE HB H 1.42 0.02 1 492 98 99 ILE HG12 H -2.12 0.02 2 493 98 99 ILE HG13 H 0.66 0.02 2 494 98 99 ILE HG2 H -0.15 0.02 1 495 99 100 VAL H H 8.29 0.02 1 496 99 100 VAL HA H 3.98 0.02 1 497 99 100 VAL HB H 2.41 0.02 1 498 99 100 VAL HG1 H 1.14 0.02 2 499 99 100 VAL HG2 H 1.18 0.02 2 500 100 101 SER H H 7.74 0.02 1 501 100 101 SER HA H 4.45 0.02 1 502 100 101 SER HB2 H 4.19 0.02 2 503 100 101 SER HB3 H 4.37 0.02 2 504 100 101 SER HG H 6.07 0.02 1 505 101 102 ASP H H 8.03 0.02 1 506 101 102 ASP HA H 4.77 0.02 1 507 101 102 ASP HB2 H 3.03 0.02 2 508 102 103 GLY H H 8.30 0.02 1 509 102 103 GLY HA2 H 4.16 0.02 2 510 102 103 GLY HA3 H 3.98 0.02 2 511 103 104 ASN H H 8.29 0.02 1 512 103 104 ASN HA H 4.90 0.02 1 513 103 104 ASN HB2 H 2.83 0.02 2 514 103 104 ASN HB3 H 2.74 0.02 2 515 103 104 ASN HD21 H 7.56 0.02 2 516 103 104 ASN HD22 H 6.95 0.02 2 517 104 105 GLY H H 8.45 0.02 1 518 104 105 GLY HA2 H 4.08 0.02 2 519 104 105 GLY HA3 H 3.88 0.02 2 520 106 107 ASN H H 7.88 0.02 1 521 106 107 ASN HA H 4.48 0.02 1 522 106 107 ASN HB2 H 2.95 0.02 2 523 106 107 ASN HD21 H 7.02 0.02 2 524 106 107 ASN HD22 H 7.51 0.02 2 525 107 108 ALA H H 7.19 0.02 1 526 107 108 ALA HA H 3.98 0.02 1 527 107 108 ALA HB H 0.78 0.02 1 528 108 109 TRP H H 7.84 0.02 1 529 108 109 TRP HA H 4.71 0.02 1 530 108 109 TRP HB2 H 3.48 0.02 2 531 108 109 TRP HB3 H 3.34 0.02 2 532 108 109 TRP HD1 H 7.14 0.02 1 533 108 109 TRP HE1 H 9.96 0.02 1 534 108 109 TRP HE3 H 7.48 0.02 1 535 108 109 TRP HZ2 H 7.09 0.02 1 536 108 109 TRP HZ3 H 6.66 0.02 1 537 108 109 TRP HH2 H 7.20 0.02 1 538 109 110 VAL HA H 3.55 0.02 1 539 109 110 VAL HB H 2.09 0.02 1 540 109 110 VAL HG1 H 1.07 0.02 2 541 109 110 VAL HG2 H 0.95 0.02 2 542 110 111 ALA HA H 4.35 0.02 1 543 110 111 ALA HB H 1.35 0.02 1 544 111 112 TRP HD1 H 6.81 0.02 1 545 111 112 TRP HE1 H 9.76 0.02 1 546 112 113 ARG H H 8.42 0.02 1 547 112 113 ARG HA H 3.51 0.02 1 548 112 113 ARG HB2 H 2.12 0.02 2 549 112 113 ARG HB3 H 1.96 0.02 2 550 113 114 ASN H H 8.06 0.02 1 551 113 114 ASN HA H 4.48 0.02 1 552 113 114 ASN HB2 H 2.78 0.02 2 553 113 114 ASN HD21 H 7.64 0.02 2 554 113 114 ASN HD22 H 6.87 0.02 2 555 114 115 ARG HA H 4.12 0.02 1 556 114 115 ARG HB2 H 1.03 0.02 2 557 114 115 ARG HB3 H 0.66 0.02 2 558 114 115 ARG HG2 H 1.58 0.02 2 559 114 115 ARG HG3 H 1.65 0.02 2 560 114 115 ARG HD2 H 3.22 0.02 2 561 114 115 ARG HE H 7.10 0.02 1 562 116 117 LYS H H 7.49 0.02 1 563 116 117 LYS HA H 3.83 0.02 1 564 117 118 GLY H H 8.64 0.02 1 565 117 118 GLY HA2 H 4.08 0.02 2 566 117 118 GLY HA3 H 3.89 0.02 2 567 118 119 THR H H 7.74 0.02 1 568 118 119 THR HA H 4.51 0.02 1 569 118 119 THR HB H 4.43 0.02 1 570 118 119 THR HG2 H 0.91 0.02 1 571 119 120 ASP H H 8.61 0.02 1 572 119 120 ASP HA H 4.90 0.02 1 573 119 120 ASP HB2 H 2.90 0.02 2 574 119 120 ASP HB3 H 2.72 0.02 2 575 120 121 VAL H H 8.34 0.02 1 576 120 121 VAL HA H 4.26 0.02 1 577 120 121 VAL HB H 2.19 0.02 1 578 120 121 VAL HG1 H 1.03 0.02 2 579 120 121 VAL HG2 H 1.06 0.02 2 580 121 122 GLN H H 8.36 0.02 1 581 121 122 GLN HA H 4.26 0.02 1 582 121 122 GLN HB2 H 2.18 0.02 2 583 121 122 GLN HE21 H 6.90 0.02 2 584 121 122 GLN HE22 H 7.70 0.02 2 585 122 123 ALA H H 7.77 0.02 1 586 122 123 ALA HA H 3.83 0.02 1 587 122 123 ALA HB H 1.16 0.02 1 588 123 124 TRP H H 7.51 0.02 1 589 123 124 TRP HA H 4.20 0.02 1 590 123 124 TRP HB2 H 3.35 0.02 2 591 123 124 TRP HD1 H 7.54 0.02 1 592 123 124 TRP HE1 H 10.48 0.02 1 593 123 124 TRP HE3 H 7.58 0.02 1 594 123 124 TRP HZ2 H 7.54 0.02 1 595 123 124 TRP HZ3 H 7.12 0.02 1 596 123 124 TRP HH2 H 7.14 0.02 1 597 124 125 ILE H H 7.42 0.02 1 598 124 125 ILE HA H 4.74 0.02 1 599 124 125 ILE HB H 2.19 0.02 1 600 124 125 ILE HG12 H 1.20 0.02 2 601 124 125 ILE HG13 H 1.41 0.02 2 602 124 125 ILE HG2 H 0.83 0.02 1 603 124 125 ILE HD1 H 0.93 0.02 1 604 125 126 ARG H H 7.36 0.02 1 605 125 126 ARG HA H 4.15 0.02 1 606 125 126 ARG HB2 H 1.97 0.02 2 607 125 126 ARG HB3 H 1.85 0.02 2 608 125 126 ARG HG2 H 1.58 0.02 2 609 125 126 ARG HG3 H 1.65 0.02 2 610 125 126 ARG HD2 H 3.14 0.02 2 611 125 126 ARG HD3 H 3.20 0.02 2 612 125 126 ARG HE H 7.34 0.02 1 613 126 127 GLY H H 9.18 0.02 1 614 126 127 GLY HA2 H 4.29 0.02 2 615 126 127 GLY HA3 H 3.76 0.02 2 616 127 128 CYS H H 7.49 0.02 1 617 127 128 CYS HA H 4.90 0.02 1 618 127 128 CYS HB2 H 3.08 0.02 2 619 127 128 CYS HB3 H 2.63 0.02 2 620 128 129 ARG H H 8.96 0.02 1 621 128 129 ARG HA H 4.35 0.02 1 622 128 129 ARG HB2 H 1.80 0.02 2 623 128 129 ARG HG2 H 1.58 0.02 2 624 128 129 ARG HG3 H 1.65 0.02 2 625 128 129 ARG HD2 H 3.21 0.02 2 626 128 129 ARG HE H 7.17 0.02 1 627 129 130 LEU H H 8.02 0.02 1 628 129 130 LEU HA H 4.28 0.02 1 629 129 130 LEU HB2 H 1.64 0.02 2 630 129 130 LEU HG H 1.40 0.02 1 631 129 130 LEU HD1 H 0.74 0.02 2 632 129 130 LEU HD2 H 0.85 0.02 2 stop_ save_