data_6556 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of 1-26 fragment of human programmed cell death 5 protein ; _BMRB_accession_number 6556 _BMRB_flat_file_name bmr6556.str _Entry_type original _Submission_date 2005-03-18 _Accession_date 2005-03-31 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Liu D. S. . 2 Feng Y. G. . 3 Yao H. W. . 4 Wang J. F. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 135 "13C chemical shifts" 43 "15N chemical shifts" 24 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2006-08-11 original author . stop_ _Original_release_date 2006-08-11 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; The N-terminal 26-residue fragment of human programmed cell death 5 protein can form a stable alpha-helix having unique electrostatic potential character ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16083422 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Liu D. S. . 2 Yao H. W. . 3 Chen Y. . . 4 Feng Y. G. . 5 Chen Y. . . 6 Wang J. F. . stop_ _Journal_abbreviation 'Biochem. J.' _Journal_volume 392 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 47 _Page_last 54 _Year 2005 _Details . loop_ _Keyword PDCD5(1-26) 'solution structure' stop_ save_ ################################## # Molecular system description # ################################## save_system_PDCD5 _Saveframe_category molecular_system _Mol_system_name 'Programmed cell death protein 5' _Abbreviation_common 'Programmed cell death protein 5' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Programmed cell death protein 5' $PDCD5 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_PDCD5 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Programmed cell death protein 5' _Abbreviation_common 'Programmed cell death protein 5' _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 27 _Mol_residue_sequence ; GSADEELEALRRQRLAELQA KHGDPGD ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 0 GLY 2 1 SER 3 2 ALA 4 3 ASP 5 4 GLU 6 5 GLU 7 6 LEU 8 7 GLU 9 8 ALA 10 9 LEU 11 10 ARG 12 11 ARG 13 12 GLN 14 13 ARG 15 14 LEU 16 15 ALA 17 16 GLU 18 17 LEU 19 18 GLN 20 19 ALA 21 20 LYS 22 21 HIS 23 22 GLY 24 23 ASP 25 24 PRO 26 25 GLY 27 26 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15864 pdcd5 100.00 113 100.00 100.00 4.90e-09 PDB 1YYB "Solution Structure Of 1-26 Fragment Of Human Programmed Cell Death 5 Protein" 100.00 27 100.00 100.00 2.05e-08 PDB 2CRU "Solution Structure Of Programmed Cell Death 5" 66.67 118 100.00 100.00 2.72e-03 PDB 2K6B "Solution Structure Of 1-112 Fragment Of Human Programmed Cell Death 5 Protein" 100.00 113 100.00 100.00 4.90e-09 DBJ BAG56975 "unnamed protein product [Homo sapiens]" 92.59 129 100.00 100.00 2.87e-07 DBJ BAJ20402 "programmed cell death 5 [synthetic construct]" 92.59 125 100.00 100.00 1.76e-07 EMBL CAG33215 "PDCD5 [Homo sapiens]" 92.59 125 100.00 100.00 1.77e-07 EMBL CAH90780 "hypothetical protein [Pongo abelii]" 92.59 125 100.00 100.00 1.76e-07 GB AAD11579 "TFAR19 [Homo sapiens]" 92.59 125 100.00 100.00 1.76e-07 GB AAH15519 "Programmed cell death 5 [Homo sapiens]" 92.59 125 100.00 100.00 1.76e-07 GB AAP35340 "programmed cell death 5 [Homo sapiens]" 92.59 125 100.00 100.00 1.76e-07 GB AAP36696 "Homo sapiens programmed cell death 5 [synthetic construct]" 92.59 126 100.00 100.00 1.84e-07 GB AAX36432 "programmed cell death 5 [synthetic construct]" 92.59 125 100.00 100.00 1.76e-07 REF NP_001125439 "programmed cell death protein 5 [Pongo abelii]" 92.59 125 100.00 100.00 1.76e-07 REF NP_001252621 "programmed cell death protein 5 [Macaca mulatta]" 92.59 125 100.00 100.00 1.76e-07 REF NP_004699 "programmed cell death protein 5 [Homo sapiens]" 92.59 125 100.00 100.00 1.76e-07 REF XP_003253507 "PREDICTED: programmed cell death protein 5-like [Nomascus leucogenys]" 88.89 194 100.00 100.00 1.64e-06 REF XP_003275098 "PREDICTED: programmed cell death protein 5 [Nomascus leucogenys]" 92.59 125 100.00 100.00 1.76e-07 SP O14737 "RecName: Full=Programmed cell death protein 5; AltName: Full=TF-1 cell apoptosis-related protein 19; Short=Protein TFAR19 [Homo" 92.59 125 100.00 100.00 1.76e-07 SP Q5RBT0 "RecName: Full=Programmed cell death protein 5 [Pongo abelii]" 92.59 125 100.00 100.00 1.76e-07 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $PDCD5 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $PDCD5 'recombinant technology' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $PDCD5 . mM 2 4 [U-15N] 'Phosphate buffer' 50 mM . . . NaCl 200 mM . . . DSS 0.02 mM . . . NaN3 0.01 mM . . . H2O 90 % . . . D2O 10 % . . . stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $PDCD5 2 mM [U-13C] 'Phosphate buffer' 50 mM . NaCl 200 mM . DSS 0.02 mM . NaN3 0.01 mM . D2O 100 % . stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $PDCD5 . mM 2 4 . 'Phosphate buffer' 50 mM . . . NaCl 200 mM . . . DSS 0.02 mM . . . NaN3 0.01 mM . . . H2O 90 % . . . D2O 10 % . . . stop_ save_ ############################ # Computer software used # ############################ save_XWINNMR _Saveframe_category software _Name XWINNMR _Version 3.5 loop_ _Task collection stop_ _Details Bruker save_ save_FELIX _Saveframe_category software _Name FELIX _Version 98 loop_ _Task 'data analysis' stop_ _Details MSI save_ save_CNS _Saveframe_category software _Name CNS _Version 1.1 loop_ _Task 'structure solution' refinement stop_ _Details Brunger save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_15N_NOESY-HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 15N NOESY-HSQC' _Sample_label . save_ save_3D_15N_TOCSY-HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 15N TOCSY-HSQC' _Sample_label . save_ save_2D_15N_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 15N HSQC' _Sample_label . save_ save_2D_13C_HMQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 13C HMQC' _Sample_label . save_ save_HMQC-NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name HMQC-NOESY _Sample_label . save_ save_HMQC-TOCSY_6 _Saveframe_category NMR_applied_experiment _Experiment_name HMQC-TOCSY _Sample_label . save_ save_2D_TOCSY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _Sample_label . save_ save_2D_NOESY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 15N NOESY-HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 15N TOCSY-HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 15N HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 13C HMQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_5 _Saveframe_category NMR_applied_experiment _Experiment_name HMQC-NOESY _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_6 _Saveframe_category NMR_applied_experiment _Experiment_name HMQC-TOCSY _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH temperature 298 . K 'ionic strength' 0.25 . M pressure 1 . atm stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 DSS C 13 'methyl protons' ppm 0.0 . indirect . . . 0.251449530 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'Programmed cell death protein 5' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 2 SER HA H 4.476 0.02 1 2 . 2 SER HB2 H 4.021 0.02 2 3 . 2 SER HB3 H 3.934 0.02 2 4 . 2 SER CA C 58.903 0.5 1 5 . 2 SER CB C 63.840 0.5 1 6 . 3 ALA H H 8.777 0.02 1 7 . 3 ALA HA H 4.318 0.02 1 8 . 3 ALA HB H 1.455 0.02 1 9 . 3 ALA CA C 54.018 0.5 1 10 . 3 ALA CB C 18.549 0.5 1 11 . 3 ALA N N 125.641 0.3 1 12 . 4 ASP H H 8.209 0.02 1 13 . 4 ASP HA H 4.496 0.02 1 14 . 4 ASP HB2 H 2.645 0.02 2 15 . 4 ASP HB3 H 2.750 0.02 2 16 . 4 ASP CA C 56.004 0.5 1 17 . 4 ASP CB C 40.731 0.5 1 18 . 4 ASP N N 118.092 0.3 1 19 . 5 GLU H H 8.182 0.02 1 20 . 5 GLU HA H 4.128 0.02 1 21 . 5 GLU HB2 H 2.104 0.02 1 22 . 5 GLU HB3 H 2.104 0.02 1 23 . 5 GLU HG2 H 2.363 0.02 1 24 . 5 GLU HG3 H 2.363 0.02 1 25 . 5 GLU CA C 58.706 0.5 1 26 . 5 GLU CB C 29.555 0.5 1 27 . 5 GLU CG C 36.448 0.5 1 28 . 5 GLU N N 121.240 0.3 1 29 . 6 GLU H H 8.281 0.02 1 30 . 6 GLU HA H 4.176 0.02 1 31 . 6 GLU HB2 H 2.104 0.02 1 32 . 6 GLU HB3 H 2.104 0.02 1 33 . 6 GLU HG2 H 2.366 0.02 2 34 . 6 GLU HG3 H 2.262 0.02 2 35 . 6 GLU CA C 58.573 0.5 1 36 . 6 GLU N N 121.238 0.3 1 37 . 7 LEU H H 8.011 0.02 1 38 . 7 LEU HA H 4.197 0.02 1 39 . 7 LEU HB2 H 1.677 0.02 2 40 . 7 LEU HB3 H 1.852 0.02 2 41 . 7 LEU HD1 H 0.966 0.02 2 42 . 7 LEU CA C 57.573 0.5 1 43 . 7 LEU N N 121.169 0.3 1 44 . 8 GLU H H 8.145 0.02 1 45 . 8 GLU HA H 4.207 0.02 1 46 . 8 GLU HB2 H 2.069 0.02 1 47 . 8 GLU HB3 H 2.069 0.02 1 48 . 8 GLU HG2 H 2.352 0.02 1 49 . 8 GLU HG3 H 2.352 0.02 1 50 . 8 GLU CA C 58.658 0.5 1 51 . 8 GLU N N 120.643 0.3 1 52 . 9 ALA H H 8.063 0.02 1 53 . 9 ALA HA H 4.105 0.02 1 54 . 9 ALA HB H 1.518 0.02 1 55 . 9 ALA CA C 55.148 0.5 1 56 . 9 ALA CB C 17.880 0.5 1 57 . 9 ALA N N 122.218 0.3 1 58 . 10 LEU H H 7.891 0.02 1 59 . 10 LEU HA H 4.164 0.02 1 60 . 10 LEU HB2 H 1.713 0.02 2 61 . 10 LEU HB3 H 1.859 0.02 2 62 . 10 LEU HD1 H 0.942 0.02 2 63 . 10 LEU CA C 57.624 0.5 1 64 . 10 LEU N N 119.857 0.3 1 65 . 11 ARG H H 8.118 0.02 1 66 . 11 ARG HA H 4.012 0.02 1 67 . 11 ARG HB2 H 1.967 0.02 1 68 . 11 ARG HB3 H 1.967 0.02 1 69 . 11 ARG HG2 H 1.572 0.02 2 70 . 11 ARG HG3 H 1.791 0.02 2 71 . 11 ARG HD2 H 3.226 0.02 1 72 . 11 ARG HD3 H 3.226 0.02 1 73 . 11 ARG CA C 59.448 0.5 1 74 . 11 ARG N N 120.832 0.3 1 75 . 12 ARG H H 8.226 0.02 1 76 . 12 ARG HA H 4.029 0.02 1 77 . 12 ARG HB2 H 1.922 0.02 1 78 . 12 ARG HB3 H 1.922 0.02 1 79 . 12 ARG HG2 H 1.628 0.02 1 80 . 12 ARG HG3 H 1.628 0.02 1 81 . 12 ARG HD2 H 3.236 0.02 1 82 . 12 ARG HD3 H 3.236 0.02 1 83 . 12 ARG CA C 59.342 0.5 1 84 . 12 ARG N N 118.536 0.3 1 85 . 13 GLN H H 8.093 0.02 1 86 . 13 GLN HA H 4.158 0.02 1 87 . 13 GLN HB2 H 2.222 0.02 1 88 . 13 GLN HB3 H 2.222 0.02 1 89 . 13 GLN HG2 H 2.451 0.02 1 90 . 13 GLN HG3 H 2.451 0.02 1 91 . 13 GLN CA C 58.452 0.5 1 92 . 13 GLN N N 120.796 0.3 1 93 . 14 ARG H H 8.216 0.02 1 94 . 14 ARG HA H 4.180 0.02 1 95 . 14 ARG HB2 H 1.960 0.02 1 96 . 14 ARG HB3 H 1.960 0.02 1 97 . 14 ARG HG2 H 1.750 0.02 1 98 . 14 ARG HG3 H 1.750 0.02 1 99 . 14 ARG HD2 H 3.223 0.02 1 100 . 14 ARG HD3 H 3.223 0.02 1 101 . 14 ARG CA C 58.573 0.5 1 102 . 14 ARG N N 119.987 0.3 1 103 . 15 LEU H H 7.973 0.02 1 104 . 15 LEU HA H 4.182 0.02 1 105 . 15 LEU HB2 H 1.788 0.02 2 106 . 15 LEU HB3 H 1.644 0.02 2 107 . 15 LEU HD1 H 0.908 0.02 2 108 . 15 LEU CA C 56.845 0.5 1 109 . 15 LEU N N 120.046 0.3 1 110 . 16 ALA H H 7.935 0.02 1 111 . 16 ALA HA H 4.205 0.02 1 112 . 16 ALA HB H 1.516 0.02 1 113 . 16 ALA CA C 54.401 0.5 1 114 . 16 ALA CB C 18.090 0.5 1 115 . 16 ALA N N 122.150 0.3 1 116 . 17 GLU H H 8.045 0.02 1 117 . 17 GLU HA H 4.134 0.02 1 118 . 17 GLU HB2 H 2.136 0.02 1 119 . 17 GLU HB3 H 2.136 0.02 1 120 . 17 GLU HG2 H 2.470 0.02 2 121 . 17 GLU HG3 H 2.277 0.02 2 122 . 17 GLU CA C 58.273 0.5 1 123 . 17 GLU N N 118.925 0.3 1 124 . 18 LEU H H 7.909 0.02 1 125 . 18 LEU HA H 4.176 0.02 1 126 . 18 LEU HB2 H 1.831 0.02 2 127 . 18 LEU HB3 H 1.679 0.02 2 128 . 18 LEU HD1 H 0.909 0.02 2 129 . 18 LEU CA C 57.477 0.5 1 130 . 18 LEU N N 120.628 0.3 1 131 . 19 GLN H H 8.071 0.02 1 132 . 19 GLN HA H 4.182 0.02 1 133 . 19 GLN HB2 H 2.114 0.02 1 134 . 19 GLN HB3 H 2.114 0.02 1 135 . 19 GLN HG2 H 2.476 0.02 1 136 . 19 GLN HG3 H 2.476 0.02 1 137 . 19 GLN CA C 56.854 0.5 1 138 . 19 GLN N N 118.908 0.3 1 139 . 20 ALA H H 7.913 0.02 1 140 . 20 ALA HA H 4.250 0.02 1 141 . 20 ALA HB H 1.431 0.02 1 142 . 20 ALA CA C 53.094 0.5 1 143 . 20 ALA CB C 18.796 0.5 1 144 . 20 ALA N N 122.990 0.3 1 145 . 21 LYS H H 7.966 0.02 1 146 . 21 LYS HA H 4.251 0.02 1 147 . 21 LYS HB2 H 1.785 0.02 1 148 . 21 LYS HB3 H 1.785 0.02 1 149 . 21 LYS HG2 H 1.453 0.02 2 150 . 21 LYS HG3 H 1.377 0.02 2 151 . 21 LYS HD2 H 1.668 0.02 1 152 . 21 LYS HD3 H 1.668 0.02 1 153 . 21 LYS HE2 H 2.980 0.02 1 154 . 21 LYS HE3 H 2.980 0.02 1 155 . 21 LYS CA C 56.523 0.5 1 156 . 21 LYS CB C 32.820 0.5 1 157 . 21 LYS CG C 24.785 0.5 1 158 . 21 LYS CD C 28.971 0.5 1 159 . 21 LYS CE C 41.900 0.5 1 160 . 21 LYS N N 119.221 0.3 1 161 . 22 HIS H H 8.212 0.02 1 162 . 22 HIS HA H 4.659 0.02 1 163 . 22 HIS HB2 H 3.127 0.02 2 164 . 22 HIS HB3 H 3.233 0.02 2 165 . 22 HIS HE1 H 7.963 0.02 1 166 . 22 HIS CA C 56.154 0.5 1 167 . 22 HIS CB C 30.392 0.5 1 168 . 22 HIS N N 119.726 0.3 1 169 . 23 GLY H H 8.291 0.02 1 170 . 23 GLY HA2 H 3.948 0.02 1 171 . 23 GLY HA3 H 3.948 0.02 1 172 . 23 GLY CA C 44.932 0.5 1 173 . 23 GLY N N 109.967 0.3 1 174 . 24 ASP H H 8.321 0.02 1 175 . 24 ASP HA H 4.916 0.02 1 176 . 24 ASP HB2 H 2.546 0.02 2 177 . 24 ASP HB3 H 2.742 0.02 2 178 . 24 ASP CA C 52.428 0.5 1 179 . 24 ASP CB C 41.284 0.5 1 180 . 24 ASP N N 122.189 0.3 1 181 . 25 PRO HA H 4.440 0.02 1 182 . 25 PRO HB2 H 2.298 0.02 2 183 . 25 PRO HB3 H 2.026 0.02 2 184 . 25 PRO HG2 H 2.055 0.02 1 185 . 25 PRO HG3 H 2.055 0.02 1 186 . 25 PRO HD2 H 3.865 0.02 2 187 . 25 PRO HD3 H 3.784 0.02 2 188 . 25 PRO CA C 63.769 0.5 1 189 . 25 PRO CB C 32.154 0.5 1 190 . 25 PRO CG C 27.240 0.5 1 191 . 25 PRO CD C 50.754 0.5 1 192 . 26 GLY H H 8.490 0.02 1 193 . 26 GLY HA2 H 3.934 0.02 1 194 . 26 GLY HA3 H 3.934 0.02 1 195 . 26 GLY CA C 45.104 0.5 1 196 . 26 GLY N N 109.540 0.3 1 197 . 27 ASP H H 7.868 0.02 1 198 . 27 ASP HA H 4.404 0.02 1 199 . 27 ASP HB2 H 2.566 0.02 2 200 . 27 ASP HB3 H 2.675 0.02 2 201 . 27 ASP CA C 55.850 0.5 1 202 . 27 ASP N N 126.090 0.3 1 stop_ save_