data_6879 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignment for aKv1.1N ; _BMRB_accession_number 6879 _BMRB_flat_file_name bmr6879.str _Entry_type original _Submission_date 2005-10-27 _Accession_date 2005-10-27 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Backbone assignment for N-terminus of the Shaker Potassium channel from Aplysia.' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Baker Kent A. . 2 Hilty Christian . . 3 Peti Wolfgang . . 4 Pfaffinger Paul . . 5 Wider Gerhard . . 6 Wuthrich Kurt . . 7 Choe Senyon . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 142 "13C chemical shifts" 142 "15N chemical shifts" 142 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2006-02-17 original author . stop_ _Original_release_date 2006-02-17 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; NMR-Derived Dynamic Aspects of N-Type Inactivation of a Kv Channel Suggest a Transient Interaction with the T1 Domain ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16460013 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Baker Kent A. . 2 Hilty Christian . . 3 Peti Wolfgang . . 4 Pfaffinger Paul . . 5 Wider Gerhard . . 6 Wuthrich Kurt . . 7 Choe Senyon . . stop_ _Journal_abbreviation Biochemistry _Journal_volume 45 _Journal_issue 6 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1663 _Page_last 1672 _Year 2006 _Details . loop_ _Keyword 'Ion channel' Shaker stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name aKv1.1N _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'aKv1.1N, chain 1' $Aplysia_Kv1_1_N-terminus 'aKv1.1N, chain 2' $Aplysia_Kv1_1_N-terminus 'aKv1.1N, chain 3' $Aplysia_Kv1_1_N-terminus 'aKv1.1N, chain 4' $Aplysia_Kv1_1_N-terminus stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state 'not present' loop_ _Magnetic_equivalence_ID _Magnetically_equivalent_system_component 1 'aKv1.1N, chain 1' 1 'aKv1.1N, chain 2' 1 'aKv1.1N, chain 3' 1 'aKv1.1N, chain 4' stop_ loop_ _Biological_function 'Potassium channel' stop_ _Database_query_date . _Details 'Symmetric Homotetramer' save_ ######################## # Monomeric polymers # ######################## save_Aplysia_Kv1_1_N-terminus _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common aKv1.1 _Molecular_mass 21012 _Mol_thiol_state 'not present' loop_ _Biological_function 'Potassium Channel Tetramerization Domain' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 181 _Mol_residue_sequence ; MEVAMAGIEGNGGPAGYRDS YHSSQRPLLRSSNLPNSRSF PKLSEEDNANENGMGVPGSD YDCSCERVVINVSGLRFETQ LKTLNQFPDTLLGNPQKRNR YYDPLRNEYFFDRNRPSFDA ILYFYQSGGRLRRPVNVPLD VFSEEIKFYELGENAFERYR EDEGFIKEEEKPLPQNEFQR R ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLU 3 VAL 4 ALA 5 MET 6 ALA 7 GLY 8 ILE 9 GLU 10 GLY 11 ASN 12 GLY 13 GLY 14 PRO 15 ALA 16 GLY 17 TYR 18 ARG 19 ASP 20 SER 21 TYR 22 HIS 23 SER 24 SER 25 GLN 26 ARG 27 PRO 28 LEU 29 LEU 30 ARG 31 SER 32 SER 33 ASN 34 LEU 35 PRO 36 ASN 37 SER 38 ARG 39 SER 40 PHE 41 PRO 42 LYS 43 LEU 44 SER 45 GLU 46 GLU 47 ASP 48 ASN 49 ALA 50 ASN 51 GLU 52 ASN 53 GLY 54 MET 55 GLY 56 VAL 57 PRO 58 GLY 59 SER 60 ASP 61 TYR 62 ASP 63 CYS 64 SER 65 CYS 66 GLU 67 ARG 68 VAL 69 VAL 70 ILE 71 ASN 72 VAL 73 SER 74 GLY 75 LEU 76 ARG 77 PHE 78 GLU 79 THR 80 GLN 81 LEU 82 LYS 83 THR 84 LEU 85 ASN 86 GLN 87 PHE 88 PRO 89 ASP 90 THR 91 LEU 92 LEU 93 GLY 94 ASN 95 PRO 96 GLN 97 LYS 98 ARG 99 ASN 100 ARG 101 TYR 102 TYR 103 ASP 104 PRO 105 LEU 106 ARG 107 ASN 108 GLU 109 TYR 110 PHE 111 PHE 112 ASP 113 ARG 114 ASN 115 ARG 116 PRO 117 SER 118 PHE 119 ASP 120 ALA 121 ILE 122 LEU 123 TYR 124 PHE 125 TYR 126 GLN 127 SER 128 GLY 129 GLY 130 ARG 131 LEU 132 ARG 133 ARG 134 PRO 135 VAL 136 ASN 137 VAL 138 PRO 139 LEU 140 ASP 141 VAL 142 PHE 143 SER 144 GLU 145 GLU 146 ILE 147 LYS 148 PHE 149 TYR 150 GLU 151 LEU 152 GLY 153 GLU 154 ASN 155 ALA 156 PHE 157 GLU 158 ARG 159 TYR 160 ARG 161 GLU 162 ASP 163 GLU 164 GLY 165 PHE 166 ILE 167 LYS 168 GLU 169 GLU 170 GLU 171 LYS 172 PRO 173 LEU 174 PRO 175 GLN 176 ASN 177 GLU 178 PHE 179 GLN 180 ARG 181 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1A68 "Crystal Structure Of The Tetramerization Domain Of The Shaker Potassium Channel" 52.49 95 98.95 98.95 1.43e-58 PDB 1EOD "Crystal Structure Of The N136d Mutant Of A Shaker T1 Domain" 55.25 100 99.00 100.00 3.13e-62 PDB 1EOE "Crystal Structure Of The V135r Mutant Of A Shaker T1 Domain" 55.25 100 99.00 99.00 9.39e-62 PDB 1EOF "Crystal Structure Of The N136a Mutant Of A Shaker T1 Domain" 55.25 100 99.00 99.00 4.78e-62 PDB 1T1D "Crystal Structure Of The Tetramerization Domain Of The Shaker Potassium Channel" 55.25 100 100.00 100.00 7.70e-63 GB AAA27756 "potassium channel [Aplysia californica]" 100.00 515 100.00 100.00 2.10e-125 REF NP_001191634 "potassium channel [Aplysia californica]" 100.00 515 100.00 100.00 2.10e-125 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Aplysia_Kv1_1_N-terminus 'California sea hare' 6500 Eukaryota Metazoa Aplysia 'Aplysia californica' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name _Vendor_name _Details $Aplysia_Kv1_1_N-terminus 'recombinant technology' 'Escherichia coli' . . . 'pET system' pET-28a Novagen 'N-terminal Thrombin cleavable His tag' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'Concentrated Protein Sample.' loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $Aplysia_Kv1_1_N-terminus 1.0 mM 0.5 1.5 '[U-2H; U-13C; U-15N]' 'Sodium Phosphate' 20 mM . . . NaCl 50 mM . . . stop_ save_ ############################ # Computer software used # ############################ save_Xwinnmr _Saveframe_category software _Name xwinnmr _Version . loop_ _Vendor _Address _Electronic_address Bruker . . stop_ loop_ _Task Collection stop_ _Details . save_ save_Xeasy _Saveframe_category software _Name XEASY _Version . loop_ _Task Processing stop_ _Details . save_ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Task Processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_900MHz_spectromer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model . _Field_strength 900 _Details . save_ save_800MHz_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model . _Field_strength 800 _Details . save_ save_500MHz_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model . _Field_strength 500 _Details Cryoprobe save_ ############################# # NMR applied experiments # ############################# save_(15N_1H)TROSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name (15N_1H)TROSY _Sample_label $sample_1 save_ save_(1H_1H)NOESY-(15N_1N)-TROSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name (1H_1H)NOESY-(15N_1N)-TROSY _Sample_label $sample_1 save_ save_TROSY-HNCA_3 _Saveframe_category NMR_applied_experiment _Experiment_name TROSY-HNCA _Sample_label $sample_1 save_ save_(H)N(COCA)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name (H)N(COCA)NH _Sample_label $sample_1 save_ save_(15N_1H)TROSY _Saveframe_category NMR_applied_experiment _Experiment_name (15N_1H)TROSY _BMRB_pulse_sequence_accession_number . _Details . save_ save_(1H_1H)NOESY-(15N_1N)-TROSY _Saveframe_category NMR_applied_experiment _Experiment_name (1H_1H)NOESY-(15N_1N)-TROSY _BMRB_pulse_sequence_accession_number . _Details . save_ save_TROSY-HNCA _Saveframe_category NMR_applied_experiment _Experiment_name TROSY-HNCA _BMRB_pulse_sequence_accession_number . _Details . save_ save_(H)N(COCA)NH _Saveframe_category NMR_applied_experiment _Experiment_name (H)N(COCA)NH _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_conditions_1 _Saveframe_category sample_conditions _Details ; 20mM Sodium Phosphate pH7.0 50mM NaCl 310K ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.0 0.1 pH temperature 310 0.1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_referencing _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.0 . indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $Xwinnmr $Xwinnmr $Xwinnmr $Xwinnmr stop_ loop_ _Experiment_label (15N_1H)TROSY (1H_1H)NOESY-(15N_1N)-TROSY TROSY-HNCA (H)N(COCA)NH stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $conditions_1 _Chem_shift_reference_set_label $chemical_shift_referencing _Mol_system_component_name 'aKv1.1N, chain 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 GLU H H 8.478 . . 2 2 2 GLU CA C 56.594 . . 3 2 2 GLU N N 122.608 . . 4 3 3 VAL H H 7.992 . . 5 3 3 VAL CA C 62.002 . . 6 3 3 VAL N N 121.178 . . 7 4 4 ALA H H 8.203 . . 8 4 4 ALA CA C 52.258 . . 9 4 4 ALA N N 127.491 . . 10 5 5 MET H H 8.158 . . 11 5 5 MET CA C 55.149 . . 12 5 5 MET N N 120.022 . . 13 6 6 ALA H H 8.143 . . 14 6 6 ALA CA C 52.352 . . 15 6 6 ALA N N 125.286 . . 16 7 7 GLY H H 8.267 . . 17 7 7 GLY CA C 45.079 . . 18 7 7 GLY N N 108.632 . . 19 8 8 ILE H H 7.834 . . 20 8 8 ILE CA C 60.883 . . 21 8 8 ILE N N 119.992 . . 22 9 9 GLU H H 8.465 . . 23 9 9 GLU CA C 56.454 . . 24 9 9 GLU N N 124.889 . . 25 10 10 GLY H H 8.247 . . 26 10 10 GLY CA C 45.079 . . 27 10 10 GLY N N 110.513 . . 28 11 11 ASN H H 7.88 . . 29 11 11 ASN CA C 54.547 . . 30 11 11 ASN N N 124.585 . . 31 12 12 GLY H H 8.103 . . 32 12 12 GLY CA C 45.213 . . 33 12 12 GLY N N 109.399 . . 34 13 13 GLY H H 8.151 . . 35 13 13 GLY CA C 44.408 . . 36 13 13 GLY N N 109.399 . . 37 15 15 ALA H H 8.302 . . 38 15 15 ALA CA C 52.445 . . 39 15 15 ALA N N 123.932 . . 40 16 16 GLY H H 8.131 . . 41 16 16 GLY CA C 45.126 . . 42 16 16 GLY N N 108.081 . . 43 17 17 TYR H H 7.864 . . 44 17 17 TYR CA C 60.964 . . 45 17 17 TYR N N 120.627 . . 46 28 28 LEU H H 8.185 . . 47 28 28 LEU CA C 55.102 . . 48 28 28 LEU N N 122.791 . . 49 29 29 LEU H H 8.054 . . 50 29 29 LEU CA C 54.729 . . 51 29 29 LEU N N 123.323 . . 52 30 30 ARG H H 8.243 . . 53 30 30 ARG CA C 52.958 . . 54 30 30 ARG N N 125.23 . . 55 36 36 ASN H H 8.35 . . 56 36 36 ASN CA C 53.09 . . 57 36 36 ASN N N 123.597 . . 58 39 39 SER H H 7.938 . . 59 39 39 SER CA C 57.845 . . 60 39 39 SER N N 114.689 . . 61 40 40 PHE H H 7.972 . . 62 40 40 PHE CA C 58.015 . . 63 40 40 PHE N N 123.049 . . 64 42 42 LYS H H 8.229 . . 65 42 42 LYS CA C 55.906 . . 66 42 42 LYS N N 122.169 . . 67 43 43 LEU H H 8.300 . . 68 43 43 LEU CA C 55.033 . . 69 43 43 LEU N N 124.590 . . 70 44 44 SER H H 8.249 . . 71 44 44 SER CA C 57.897 . . 72 44 44 SER N N 116.621 . . 73 45 45 GLU H H 8.479 . . 74 45 45 GLU CA C 56.687 . . 75 45 45 GLU N N 123.11 . . 76 46 46 GLU H H 8.27 . . 77 46 46 GLU CA C 56.588 . . 78 46 46 GLU N N 121.068 . . 79 47 47 ASP H H 8.18 . . 80 47 47 ASP CA C 54.263 . . 81 47 47 ASP N N 121.574 . . 82 48 48 ASN H H 8.24 . . 83 48 48 ASN CA C 53.053 . . 84 48 48 ASN N N 119.862 . . 85 49 49 ALA H H 8.243 . . 86 49 49 ALA CA C 52.699 . . 87 49 49 ALA N N 124.37 . . 88 50 50 ASN H H 8.246 . . 89 50 50 ASN CA C 53.191 . . 90 50 50 ASN N N 117.644 . . 91 51 51 GLU H H 8.288 . . 92 51 51 GLU CA C 56.734 . . 93 51 51 GLU N N 121.513 . . 94 52 52 ASN H H 8.348 . . 95 52 52 ASN CA C 53.284 . . 96 52 52 ASN N N 119.307 . . 97 53 53 GLY H H 8.242 . . 98 53 53 GLY CA C 45.405 . . 99 53 53 GLY N N 109.473 . . 100 54 54 MET H H 8.112 . . 101 54 54 MET CA C 55.242 . . 102 54 54 MET N N 119.885 . . 103 55 55 GLY H H 8.324 . . 104 55 55 GLY CA C 44.986 . . 105 55 55 GLY N N 110.265 . . 106 56 56 VAL H H 7.937 . . 107 56 56 VAL CA C 59.578 . . 108 56 56 VAL N N 121.102 . . 109 58 58 GLY H H 8.432 . . 110 58 58 GLY CA C 45.079 . . 111 58 58 GLY N N 110.116 . . 112 59 59 SER H H 8.123 . . 113 59 59 SER CA C 58.179 . . 114 59 59 SER N N 115.759 . . 115 60 60 ASP H H 8.362 . . 116 60 60 ASP CA C 54.17 . . 117 60 60 ASP N N 122.274 . . 118 61 61 TYR H H 7.982 . . 119 61 61 TYR CA C 57.993 . . 120 61 61 TYR N N 121.011 . . 121 62 62 ASP H H 8.11 . . 122 62 62 ASP CA C 53.683 . . 123 62 62 ASP N N 123.38 . . 124 63 63 CYS H H 7.961 . . 125 63 63 CYS CA C 59.376 . . 126 63 63 CYS N N 122.829 . . 127 67 67 ARG H H 8.278 . . 128 67 67 ARG CA C 54.543 . . 129 67 67 ARG N N 123.49 . . 130 68 68 VAL H H 8.834 . . 131 68 68 VAL CA C 60.327 . . 132 68 68 VAL N N 121.832 . . 133 69 69 VAL H H 8.732 . . 134 69 69 VAL CA C 61.326 . . 135 69 69 VAL N N 125.017 . . 136 70 70 ILE H H 9.457 . . 137 70 70 ILE CA C 60.33 . . 138 70 70 ILE N N 128.674 . . 139 71 71 ASN H H 9.735 . . 140 71 71 ASN CA C 51.007 . . 141 71 71 ASN N N 128.591 . . 142 72 72 VAL H H 8.907 . . 143 72 72 VAL CA C 60.434 . . 144 72 72 VAL N N 126.159 . . 145 73 73 SER H H 8.628 . . 146 73 73 SER CA C 59.764 . . 147 73 73 SER N N 119.069 . . 148 74 74 GLY H H 8.987 . . 149 74 74 GLY CA C 45.492 . . 150 74 74 GLY N N 111.818 . . 151 75 75 LEU H H 8.395 . . 152 75 75 LEU CA C 53.487 . . 153 75 75 LEU N N 128.023 . . 154 76 76 ARG H H 8.135 . . 155 76 76 ARG CA C 56.766 . . 156 76 76 ARG N N 126.219 . . 157 77 77 PHE H H 9.922 . . 158 77 77 PHE CA C 56.277 . . 159 77 77 PHE N N 125.684 . . 160 78 78 GLU H H 7.583 . . 161 78 78 GLU CA C 54.134 . . 162 78 78 GLU N N 121.25 . . 163 79 79 THR H H 8.3 . . 164 79 79 THR CA C 58.853 . . 165 79 79 THR N N 114.613 . . 166 80 80 GLN H H 8.732 . . 167 80 80 GLN CA C 54.741 . . 168 80 80 GLN N N 122.117 . . 169 81 81 LEU H H 8.841 . . 170 81 81 LEU CA C 58.591 . . 171 81 81 LEU N N 126.53 . . 172 82 82 LYS H H 8.616 . . 173 82 82 LYS CA C 58.645 . . 174 82 82 LYS N N 114.878 . . 175 83 83 THR H H 7.363 . . 176 83 83 THR CA C 64.846 . . 177 83 83 THR N N 118.514 . . 178 84 84 LEU H H 6.86 . . 179 84 84 LEU CA C 56.2 . . 180 84 84 LEU N N 119.282 . . 181 85 85 ASN H H 8.051 . . 182 85 85 ASN CA C 53.533 . . 183 85 85 ASN N N 113.986 . . 184 86 86 GLN H H 7.031 . . 185 86 86 GLN CA C 57.373 . . 186 86 86 GLN N N 116.694 . . 187 87 87 PHE H H 7.211 . . 188 87 87 PHE CA C 55.382 . . 189 87 87 PHE N N 114.2 . . 190 89 89 ASP H H 8.79 . . 191 89 89 ASP CA C 53.843 . . 192 89 89 ASP N N 119.422 . . 193 90 90 THR H H 7.443 . . 194 90 90 THR CA C 59.85 . . 195 90 90 THR N N 109.213 . . 196 91 91 LEU H H 7.2 . . 197 91 91 LEU CA C 60.099 . . 198 91 91 LEU N N 121.331 . . 199 92 92 LEU H H 9.216 . . 200 92 92 LEU CA C 55.551 . . 201 92 92 LEU N N 112.481 . . 202 93 93 GLY H H 7.315 . . 203 93 93 GLY CA C 45.136 . . 204 93 93 GLY N N 104.1 . . 205 94 94 ASN H H 7.167 . . 206 94 94 ASN CA C 49.7 . . 207 94 94 ASN N N 120.004 . . 208 96 96 GLN H H 7.756 . . 209 96 96 GLN CA C 58.133 . . 210 96 96 GLN N N 113.741 . . 211 97 97 LYS H H 7.794 . . 212 97 97 LYS CA C 58.851 . . 213 97 97 LYS N N 118.734 . . 214 98 98 ARG H H 8.522 . . 215 98 98 ARG CA C 58.709 . . 216 98 98 ARG N N 117.326 . . 217 99 99 ASN H H 7.863 . . 218 99 99 ASN CA C 56.034 . . 219 99 99 ASN N N 118.2 . . 220 100 100 ARG H H 7.438 . . 221 100 100 ARG CA C 58.009 . . 222 100 100 ARG N N 119.315 . . 223 101 101 TYR H H 8.018 . . 224 101 101 TYR CA C 57.466 . . 225 101 101 TYR N N 117.325 . . 226 102 102 TYR H H 7.464 . . 227 102 102 TYR CA C 58.159 . . 228 102 102 TYR N N 122.193 . . 229 103 103 ASP H H 8.418 . . 230 103 103 ASP CA C 49.345 . . 231 103 103 ASP N N 129.327 . . 232 105 105 LEU H H 7.781 . . 233 105 105 LEU CA C 56.711 . . 234 105 105 LEU N N 119.48 . . 235 106 106 ARG H H 6.806 . . 236 106 106 ARG CA C 55.197 . . 237 106 106 ARG N N 116.053 . . 238 107 107 ASN H H 7.94 . . 239 107 107 ASN CA C 53.648 . . 240 107 107 ASN N N 118.075 . . 241 108 108 GLU H H 7.056 . . 242 108 108 GLU CA C 53.214 . . 243 108 108 GLU N N 114.353 . . 244 109 109 TYR H H 8.686 . . 245 109 109 TYR CA C 57.69 . . 246 109 109 TYR N N 120.813 . . 247 110 110 PHE H H 8.539 . . 248 110 110 PHE CA C 56.306 . . 249 110 110 PHE N N 124.387 . . 250 111 111 PHE H H 8.391 . . 251 111 111 PHE CA C 55.593 . . 252 111 111 PHE N N 124.86 . . 253 112 112 ASP H H 8.754 . . 254 112 112 ASP CA C 51.206 . . 255 112 112 ASP N N 129.508 . . 256 113 113 ARG H H 6.891 . . 257 113 113 ARG CA C 54.35 . . 258 113 113 ARG N N 116.643 . . 259 117 117 SER H H 7.809 . . 260 117 117 SER CA C 58.593 . . 261 117 117 SER N N 110.72 . . 262 120 120 ALA H H 7.134 . . 263 120 120 ALA CA C 53.657 . . 264 120 120 ALA N N 118.583 . . 265 121 121 ILE H H 7.18 . . 266 121 121 ILE CA C 61.855 . . 267 121 121 ILE N N 121.726 . . 268 122 122 LEU H H 7.766 . . 269 122 122 LEU CA C 57.527 . . 270 122 122 LEU N N 117.967 . . 271 123 123 TYR H H 7.926 . . 272 123 123 TYR CA C 60.418 . . 273 123 123 TYR N N 117.22 . . 274 124 124 PHE H H 7.618 . . 275 124 124 PHE CA C 61.17 . . 276 124 124 PHE N N 123.696 . . 277 125 125 TYR H H 7.261 . . 278 125 125 TYR CA C 61.584 . . 279 125 125 TYR N N 115.993 . . 280 126 126 GLN H H 7.842 . . 281 126 126 GLN CA C 58.534 . . 282 126 126 GLN N N 118.206 . . 283 127 127 SER H H 8.026 . . 284 127 127 SER CA C 58.079 . . 285 127 127 SER N N 110.865 . . 286 128 128 GLY H H 7.552 . . 287 128 128 GLY CA C 46.179 . . 288 128 128 GLY N N 110.544 . . 289 129 129 GLY H H 8.303 . . 290 129 129 GLY CA C 44.659 . . 291 129 129 GLY N N 107.533 . . 292 131 131 LEU H H 8.648 . . 293 131 131 LEU CA C 53.13 . . 294 131 131 LEU N N 129.715 . . 295 132 132 ARG H H 8.386 . . 296 132 132 ARG CA C 52.545 . . 297 132 132 ARG N N 126.401 . . 298 133 133 ARG H H 8.864 . . 299 133 133 ARG CA C 53.376 . . 300 133 133 ARG N N 127.385 . . 301 135 135 VAL H H 8.215 . . 302 135 135 VAL CA C 61.708 . . 303 135 135 VAL N N 119.794 . . 304 136 136 ASN H H 8.255 . . 305 136 136 ASN CA C 52.741 . . 306 136 136 ASN N N 113.175 . . 307 137 137 VAL H H 7.729 . . 308 137 137 VAL CA C 59.399 . . 309 137 137 VAL N N 123.437 . . 310 139 139 LEU H H 8.802 . . 311 139 139 LEU CA C 58.957 . . 312 139 139 LEU N N 124.447 . . 313 140 140 ASP H H 8.819 . . 314 140 140 ASP CA C 56.24 . . 315 140 140 ASP N N 117.119 . . 316 141 141 VAL H H 7.263 . . 317 141 141 VAL CA C 65.061 . . 318 141 141 VAL N N 122.404 . . 319 142 142 PHE H H 8.41 . . 320 142 142 PHE CA C 61.862 . . 321 142 142 PHE N N 119.672 . . 322 143 143 SER H H 8.538 . . 323 143 143 SER CA C 61.815 . . 324 143 143 SER N N 112.449 . . 325 144 144 GLU H H 7.263 . . 326 144 144 GLU CA C 58.689 . . 327 144 144 GLU N N 120.248 . . 328 145 145 GLU H H 7.878 . . 329 145 145 GLU CA C 57.414 . . 330 145 145 GLU N N 120.074 . . 331 146 146 ILE H H 8.055 . . 332 146 146 ILE CA C 65.717 . . 333 146 146 ILE N N 117.222 . . 334 147 147 LYS H H 7.073 . . 335 147 147 LYS CA C 58.712 . . 336 147 147 LYS N N 118.07 . . 337 148 148 PHE H H 7.96 . . 338 148 148 PHE CA C 61.992 . . 339 148 148 PHE N N 123.71 . . 340 149 149 TYR H H 8.006 . . 341 149 149 TYR CA C 60.188 . . 342 149 149 TYR N N 112.808 . . 343 150 150 GLU H H 7.721 . . 344 150 150 GLU CA C 56.315 . . 345 150 150 GLU N N 119.747 . . 346 151 151 LEU H H 7.097 . . 347 151 151 LEU CA C 55.778 . . 348 151 151 LEU N N 113.894 . . 349 152 152 GLY H H 7.748 . . 350 152 152 GLY CA C 44.38 . . 351 152 152 GLY N N 105.985 . . 352 153 153 GLU H H 8.297 . . 353 153 153 GLU CA C 57.767 . . 354 153 153 GLU N N 121.041 . . 355 154 154 ASN H H 8.448 . . 356 154 154 ASN CA C 55.27 . . 357 154 154 ASN N N 118.172 . . 358 155 155 ALA H H 7.959 . . 359 155 155 ALA CA C 53.621 . . 360 155 155 ALA N N 123.025 . . 361 156 156 PHE H H 7.984 . . 362 156 156 PHE CA C 59.345 . . 363 156 156 PHE N N 117.779 . . 364 157 157 GLU H H 8.294 . . 365 157 157 GLU CA C 57.864 . . 366 157 157 GLU N N 120.372 . . 367 158 158 ARG H H 7.892 . . 368 158 158 ARG CA C 57.463 . . 369 158 158 ARG N N 120.615 . . 370 159 159 TYR H H 8.059 . . 371 159 159 TYR CA C 57.049 . . 372 159 159 TYR N N 121.65 . . 373 160 160 ARG H H 7.915 . . 374 160 160 ARG CA C 58.598 . . 375 160 160 ARG N N 121.403 . . 376 161 161 GLU H H 8.093 . . 377 161 161 GLU CA C 57.544 . . 378 161 161 GLU N N 120.935 . . 379 162 162 ASP H H 8.214 . . 380 162 162 ASP CA C 55.452 . . 381 162 162 ASP N N 121.117 . . 382 163 163 GLU H H 8.022 . . 383 163 163 GLU CA C 56.768 . . 384 163 163 GLU N N 119.657 . . 385 164 164 GLY H H 8.069 . . 386 164 164 GLY CA C 45.37 . . 387 164 164 GLY N N 108.402 . . 388 165 165 PHE H H 7.745 . . 389 165 165 PHE CA C 57.34 . . 390 165 165 PHE N N 119.794 . . 391 166 166 ILE H H 7.937 . . 392 166 166 ILE CA C 60.599 . . 393 166 166 ILE N N 122.791 . . 394 167 167 LYS H H 8.239 . . 395 167 167 LYS CA C 56.052 . . 396 167 167 LYS N N 126.193 . . 397 168 168 GLU H H 8.415 . . 398 168 168 GLU CA C 56.408 . . 399 168 168 GLU N N 123.781 . . 400 169 169 GLU H H 8.438 . . 401 169 169 GLU CA C 56.205 . . 402 169 169 GLU N N 122.228 . . 403 170 170 GLU H H 8.313 . . 404 170 170 GLU CA C 56.137 . . 405 170 170 GLU N N 122.887 . . 406 171 171 LYS H H 8.189 . . 407 171 171 LYS CA C 53.797 . . 408 171 171 LYS N N 124.358 . . 409 173 173 LEU H H 8.237 . . 410 173 173 LEU CA C 52.771 . . 411 173 173 LEU N N 124.16 . . 412 175 175 GLN H H 8.401 . . 413 175 175 GLN CA C 55.755 . . 414 175 175 GLN N N 120.418 . . 415 178 178 PHE H H 8.081 . . 416 178 178 PHE CA C 57.713 . . 417 178 178 PHE N N 120.509 . . 418 179 179 GLN H H 8.014 . . 419 179 179 GLN CA C 57.481 . . 420 179 179 GLN N N 121.844 . . 421 180 180 ARG H H 8.14 . . 422 180 180 ARG CA C 55.848 . . 423 180 180 ARG N N 123.38 . . 424 181 181 ARG H H 7.902 . . 425 181 181 ARG CA C 57.226 . . 426 181 181 ARG N N 128.403 . . stop_ save_