data_7098 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Linear dimer of stemloop SL1 from HIV-1 ; _BMRB_accession_number 7098 _BMRB_flat_file_name bmr7098.str _Entry_type original _Submission_date 2006-05-05 _Accession_date 2006-05-09 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ulyanov N. B. . 2 Mujeeb A. . . 3 Du Z. . . 4 Tonelli M. . . 5 Parslow T. G. . 6 James T. L. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 181 "13C chemical shifts" 137 "15N chemical shifts" 11 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2007-02-07 original author . stop_ _Original_release_date 2007-02-07 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; NMR structure of the full-length linear dimer of stem-loop-1 RNA in the HIV-1 dimer initiation site ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16603544 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ulyanov N. B. . 2 Mujeeb A. . . 3 Du Z. . . 4 Tonelli M. . . 5 Parslow T. G. . 6 James T. L. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 281 _Journal_issue 23 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 16168 _Page_last 16177 _Year 2006 _Details . loop_ _Keyword 'A-RICH INTERNAL LOOP' 'G-RICH INTERNAL LOOP' 'LINEAR DIMER' stop_ save_ ################################## # Molecular system description # ################################## save_system_RNA-35-MER _Saveframe_category molecular_system _Mol_system_name 'RNA (35-MER)' _Abbreviation_common 'RNA (35-MER)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'RNA (35-MER), chain A' $RNA-35-MER 'RNA (35-MER), chain B' $RNA-35-MER stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state dimer _System_paramagnetic no _System_thiol_state 'not present' loop_ _Magnetic_equivalence_ID _Magnetically_equivalent_system_component 1 'RNA (35-MER), chain A' 1 'RNA (35-MER), chain B' stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_RNA-35-MER _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common 'RNA (35-MER)' _Abbreviation_common 'RNA (35-MER)' _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 35 _Mol_residue_sequence ; GACGGCUUGCUGAAGCGCGC ACGGCAAGAGGCGUC ; loop_ _Residue_seq_code _Residue_label 1 G 2 A 3 C 4 G 5 G 6 C 7 U 8 U 9 G 10 C 11 U 12 G 13 A 14 A 15 G 16 C 17 G 18 C 19 G 20 C 21 A 22 C 23 G 24 G 25 C 26 A 27 A 28 G 29 A 30 G 31 G 32 C 33 G 34 U 35 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $RNA-35-MER HIV-1 11676 Viruses . Lentivirus HIV-1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $RNA-35-MER 'chemical synthesis' . . . . . 'RNA WAS PREPARED BY IN VITRO TRANSCRIPTION WITH T7 RNA POLYMERASE.' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA-35-MER 0.75 mM . 'potassium phosphate buffer' 10 mM . NaCl 250 mM . MgCl2 0.1 mM . D2O 100 % . stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA-35-MER 0.75 mM . 'potassium phosphate buffer' 10 mM . NaCl 250 mM . MgCl2 0.1 mM . H2O 90 % . D2O 10 % . stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA-35-MER 0.75 mM '[U-13C; U-15N]' 'potassium phosphate buffer' 10 mM . NaCl 250 mM . MgCl2 0.1 mM . D2O 100 % . stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA-35-MER 0.75 mM '[U-13C; U-15N]' 'potassium phosphate buffer' 10 mM . NaCl 250 mM . MgCl2 0.1 mM . H2O 90 % . D2O 10 % . stop_ save_ ############################ # Computer software used # ############################ save_VNMR _Saveframe_category software _Name VNMR _Version 6.1 loop_ _Task collection stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version 2.3 loop_ _Task processing stop_ _Details Delaglio save_ save_MARDIGRAS _Saveframe_category software _Name MARDIGRAS _Version 3.21 loop_ _Task 'iterative matrix relaxation' stop_ _Details 'Borgias et al' save_ save_Sparky _Saveframe_category software _Name SPARKY _Version 3.110 loop_ _Task 'data analysis' stop_ _Details Goddard save_ save_DYANA _Saveframe_category software _Name DYANA _Version 1.5 loop_ _Task refinement stop_ _Details Guntert save_ save_miniCarlo _Saveframe_category software _Name miniCarlo _Version . loop_ _Task refinement stop_ _Details 'Zhurkin & Ulyanov' save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model 'Unity Inova' _Field_strength 900 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label . save_ save_2D_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _Sample_label . save_ save_3D_13C-separated_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 13C-separated NOESY' _Sample_label . save_ save_non-decoupled_constant-time_1H-13C_TROSY-HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name 'non-decoupled constant-time 1H-13C TROSY-HSQC' _Sample_label . save_ save_2D_1H-15N_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 13C-separated NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_5 _Saveframe_category NMR_applied_experiment _Experiment_name 'non-decoupled constant-time 1H-13C TROSY-HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 260 . mM pH 6.4 . pH pressure 1 . atm temperature 303 . K stop_ save_ save_sample_cond_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 260 . mM pH 6.4 . pH pressure 1 . atm temperature 288 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio TSP C 13 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 TSP H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 TSP N 15 'methyl protons' ppm 0.0 . indirect . . . 0.251449530 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 $sample_2 $sample_3 $sample_4 stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'RNA (35-MER), chain A' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 G H1' H 5.74 0.005 1 2 . 1 G H2' H 4.88 0.005 1 3 . 1 G H3' H 4.79 0.005 1 4 . 1 G H4' H 4.55 0.005 1 5 . 1 G H5'' H 4.42 0.005 1 6 . 1 G H5' H 4.27 0.005 1 7 . 1 G H8 H 8.13 0.005 1 8 . 1 G C1' C 91.3 0.1 1 9 . 1 G C2' C 74.9 0.1 1 10 . 1 G C4' C 83.5 0.1 1 11 . 1 G C8 C 139.3 0.1 1 12 . 2 A H1' H 6.11 0.005 1 13 . 2 A H2 H 7.93 0.005 1 14 . 2 A H2' H 4.59 0.005 1 15 . 2 A H3' H 4.70 0.005 1 16 . 2 A H8 H 8.20 0.005 1 17 . 2 A C1' C 92.8 0.1 1 18 . 2 A C2 C 154.1 0.1 1 19 . 2 A C2' C 75.5 0.1 1 20 . 2 A C3' C 72.9 0.1 1 21 . 2 A C8 C 140.2 0.1 1 22 . 3 C H1' H 5.48 0.005 1 23 . 3 C H5 H 5.24 0.005 1 24 . 3 C H2' H 4.44 0.005 1 25 . 3 C H3' H 4.45 0.005 1 26 . 3 C H6 H 7.52 0.005 1 27 . 3 C C1' C 93.1 0.1 1 28 . 3 C C5 C 97.5 0.1 1 29 . 3 C C2' C 75.6 0.1 1 30 . 3 C C6 C 140.1 0.1 1 31 . 4 G H1' H 5.76 0.005 1 32 . 4 G H2' H 4.76 0.005 1 33 . 4 G H8 H 7.45 0.005 1 34 . 4 G C1' C 93.0 0.1 1 35 . 4 G C8 C 136.1 0.1 1 36 . 5 G H1' H 5.72 0.005 1 37 . 5 G H2' H 4.52 0.005 1 38 . 5 G H8 H 7.42 0.005 1 39 . 5 G C1' C 93.0 0.1 1 40 . 6 C H1' H 5.46 0.005 1 41 . 6 C H5 H 5.32 0.005 1 42 . 6 C H2' H 4.29 0.005 1 43 . 6 C H6 H 7.58 0.005 1 44 . 6 C C1' C 94.3 0.1 1 45 . 6 C C5 C 97.4 0.1 1 46 . 6 C C2' C 75.2 0.1 1 47 . 6 C C6 C 140.9 0.1 1 48 . 7 U H1' H 5.47 0.005 1 49 . 7 U H5 H 5.40 0.005 1 50 . 7 U H2' H 4.41 0.005 1 51 . 7 U H6 H 7.92 0.005 1 52 . 7 U C1' C 93.7 0.1 1 53 . 7 U C5 C 103.0 0.1 1 54 . 7 U C2' C 75.1 0.1 1 55 . 7 U C6 C 142.2 0.1 1 56 . 7 U H3 H 14.14 0.005 1 57 . 7 U N3 N 163.2 0.1 1 58 . 8 U H1' H 5.59 0.005 1 59 . 8 U H5 H 5.56 0.005 1 60 . 8 U H2' H 4.50 0.005 1 61 . 8 U H3' H 4.59 0.005 1 62 . 8 U H6 H 7.94 0.005 1 63 . 8 U C1' C 93.1 0.1 1 64 . 8 U C5 C 103.7 0.1 1 65 . 8 U C2' C 75.2 0.1 1 66 . 8 U C3' C 72.6 0.1 1 67 . 8 U C6 C 142.1 0.1 1 68 . 8 U H3 H 13.09 0.005 1 69 . 8 U N3 N 162.9 0.1 1 70 . 9 G H1' H 5.74 0.005 1 71 . 9 G H2' H 4.46 0.005 1 72 . 9 G H3' H 4.54 0.005 1 73 . 9 G H8 H 7.74 0.005 1 74 . 9 G C1' C 92.5 0.1 1 75 . 9 G C3' C 72.9 0.1 1 76 . 9 G C8 C 136.3 0.1 1 77 . 9 G H1 H 12.42 0.005 1 78 . 9 G N1 N 148.2 0.1 1 79 . 10 C H1' H 5.43 0.005 1 80 . 10 C H5 H 5.12 0.005 1 81 . 10 C H2' H 4.54 0.005 1 82 . 10 C H3' H 4.25 0.005 1 83 . 10 C H6 H 7.52 0.005 1 84 . 10 C C1' C 93.6 0.1 1 85 . 10 C C5 C 97.0 0.1 1 86 . 10 C C3' C 72.0 0.1 1 87 . 10 C C6 C 140.6 0.1 1 88 . 11 U H1' H 5.58 0.005 1 89 . 11 U H5 H 5.63 0.005 1 90 . 11 U H2' H 4.19 0.005 1 91 . 11 U H4' H 4.37 0.005 1 92 . 11 U H6 H 7.70 0.005 1 93 . 11 U C1' C 94.4 0.1 1 94 . 11 U C5 C 105.0 0.1 1 95 . 11 U C2' C 75.5 0.1 1 96 . 11 U C4' C 82.7 0.1 1 97 . 11 U C6 C 141.0 0.1 1 98 . 11 U H3 H 11.62 0.005 1 99 . 11 U N3 N 159.1 0.1 1 100 . 12 G H1' H 5.65 0.005 1 101 . 12 G H2' H 4.30 0.005 1 102 . 12 G H3' H 4.70 0.005 1 103 . 12 G H4' H 4.40 0.005 1 104 . 12 G H5' H 4.10 0.005 1 105 . 12 G H8 H 7.81 0.005 1 106 . 12 G C1' C 90.9 0.1 1 107 . 12 G C2' C 76.3 0.1 1 108 . 12 G C4' C 83.8 0.1 1 109 . 12 G C8 C 137.7 0.1 1 110 . 13 A H1' H 5.92 0.005 1 111 . 13 A H2 H 7.95 0.005 1 112 . 13 A H2' H 4.63 0.005 1 113 . 13 A H3' H 4.74 0.005 1 114 . 13 A H4' H 4.51 0.005 1 115 . 13 A H5'' H 4.37 0.005 1 116 . 13 A H5' H 4.20 0.005 1 117 . 13 A H8 H 8.16 0.005 1 118 . 13 A C1' C 90.8 0.1 1 119 . 13 A C2 C 155.1 0.1 1 120 . 13 A C2' C 76.1 0.1 1 121 . 13 A C3' C 75.8 0.1 1 122 . 13 A C4' C 84.3 0.1 1 123 . 13 A C5' C 66.8 0.1 1 124 . 13 A C8 C 141.1 0.1 1 125 . 14 A H1' H 5.96 0.005 1 126 . 14 A H2 H 8.05 0.005 1 127 . 14 A H2' H 4.72 0.005 1 128 . 14 A H3' H 4.75 0.005 1 129 . 14 A H4' H 4.54 0.005 1 130 . 14 A H5'' H 4.36 0.005 1 131 . 14 A H5' H 4.23 0.005 1 132 . 14 A H8 H 8.22 0.005 1 133 . 14 A C1' C 90.5 0.1 1 134 . 14 A C2 C 155.0 0.1 1 135 . 14 A C2' C 76.1 0.1 1 136 . 14 A C4' C 84.6 0.1 1 137 . 14 A C5' C 67.2 0.1 1 138 . 14 A C8 C 141.3 0.1 1 139 . 15 G H1' H 5.57 0.005 1 140 . 15 G H2' H 4.59 0.005 1 141 . 15 G H3' H 4.47 0.005 1 142 . 15 G H4' H 4.47 0.005 1 143 . 15 G H8 H 7.52 0.005 1 144 . 15 G C1' C 92.9 0.1 1 145 . 15 G C2' C 74.9 0.1 1 146 . 15 G C3' C 73.6 0.1 1 147 . 15 G C4' C 82.8 0.1 1 148 . 15 G C8 C 137.4 0.1 1 149 . 15 G H1 H 12.48 0.005 1 150 . 15 G N1 N 148.7 0.1 1 151 . 16 C H1' H 5.50 0.005 1 152 . 16 C H5 H 5.11 0.005 1 153 . 16 C H2' H 4.52 0.005 1 154 . 16 C H6 H 7.64 0.005 1 155 . 16 C C5 C 97.0 0.1 1 156 . 16 C C6 C 140.8 0.1 1 157 . 17 G H1' H 5.68 0.005 1 158 . 17 G H2' H 4.44 0.005 1 159 . 17 G H8 H 7.48 0.005 1 160 . 17 G C1' C 93.5 0.1 1 161 . 17 G C8 C 135.8 0.1 1 162 . 17 G H1 H 12.78 0.005 1 163 . 17 G N1 N 148.3 0.1 1 164 . 18 C H1' H 5.47 0.005 1 165 . 18 C H5 H 5.13 0.005 1 166 . 18 C H2' H 4.51 0.005 1 167 . 18 C H6 H 7.60 0.005 1 168 . 18 C C1' C 93.5 0.1 1 169 . 18 C C6 C 140.4 0.1 1 170 . 19 G H1' H 5.68 0.005 1 171 . 19 G H2' H 4.50 0.005 1 172 . 19 G H8 H 7.46 0.005 1 173 . 19 G C1' C 92.8 0.1 1 174 . 19 G C2' C 75.2 0.1 1 175 . 19 G C8 C 135.8 0.1 1 176 . 19 G H1 H 12.88 0.005 1 177 . 19 G N1 N 148.3 0.1 1 178 . 20 C H1' H 5.43 0.005 1 179 . 20 C H5 H 5.13 0.005 1 180 . 20 C H2' H 4.51 0.005 1 181 . 20 C H6 H 7.44 0.005 1 182 . 20 C C1' C 93.7 0.1 1 183 . 20 C C5 C 97.3 0.1 1 184 . 20 C C2' C 75.3 0.1 1 185 . 20 C C6 C 140.3 0.1 1 186 . 21 A H1' H 5.94 0.005 1 187 . 21 A H2 H 7.66 0.005 1 188 . 21 A H2' H 4.50 0.005 1 189 . 21 A H3' H 4.53 0.005 1 190 . 21 A H8 H 7.97 0.005 1 191 . 21 A C1' C 92.7 0.1 1 192 . 21 A C2 C 154.5 0.1 1 193 . 21 A C3' C 73.1 0.1 1 194 . 21 A C8 C 139.3 0.1 1 195 . 22 C H1' H 5.29 0.005 1 196 . 22 C H5 H 5.29 0.005 1 197 . 22 C H2' H 4.40 0.005 1 198 . 22 C H6 H 7.46 0.005 1 199 . 22 C C1' C 93.0 0.1 1 200 . 22 C C5 C 97.6 0.1 1 201 . 22 C C2' C 75.6 0.1 1 202 . 22 C C6 C 140.7 0.1 1 203 . 23 G H1' H 5.60 0.005 1 204 . 23 G H2' H 4.64 0.005 1 205 . 23 G H3' H 4.31 0.005 1 206 . 23 G H8 H 7.45 0.005 1 207 . 23 G C1' C 93.2 0.1 1 208 . 23 G C2' C 74.9 0.1 1 209 . 23 G C3' C 73.4 0.1 1 210 . 23 G C8 C 137.1 0.1 1 211 . 23 G H1 H 10.39 0.005 1 212 . 23 G N1 N 143.5 0.1 1 213 . 24 G H1' H 5.63 0.005 1 214 . 24 G H2' H 4.43 0.005 1 215 . 24 G H8 H 7.17 0.005 1 216 . 24 G C1' C 92.9 0.1 1 217 . 24 G C8 C 136.0 0.1 1 218 . 24 G H1 H 13.17 0.005 1 219 . 24 G N1 N 149.3 0.1 1 220 . 25 C H1' H 5.47 0.005 1 221 . 25 C H5 H 5.15 0.005 1 222 . 25 C H2' H 4.54 0.005 1 223 . 25 C H6 H 7.56 0.005 1 224 . 25 C C5 C 97.2 0.1 1 225 . 25 C C2' C 72.0 0.1 1 226 . 25 C C6 C 140.6 0.1 1 227 . 26 A H1' H 5.86 0.005 1 228 . 26 A H2 H 6.69 0.005 1 229 . 26 A H2' H 4.60 0.005 1 230 . 26 A H3' H 4.67 0.005 1 231 . 26 A H4' H 4.50 0.005 1 232 . 26 A H8 H 7.92 0.005 1 233 . 26 A C1' C 92.9 0.1 1 234 . 26 A C2 C 152.1 0.1 1 235 . 26 A C2' C 75.6 0.1 1 236 . 26 A C3' C 73.2 0.1 1 237 . 26 A C8 C 139.4 0.1 1 238 . 27 A H1' H 5.76 0.005 1 239 . 27 A H2 H 7.33 0.005 1 240 . 27 A H2' H 4.49 0.005 1 241 . 27 A H8 H 7.68 0.005 1 242 . 27 A C1' C 92.4 0.1 1 243 . 27 A C2 C 153.0 0.1 1 244 . 27 A C2' C 75.5 0.1 1 245 . 27 A C8 C 139.0 0.1 1 246 . 28 G H1' H 5.46 0.005 1 247 . 28 G H2' H 4.14 0.005 1 248 . 28 G H3' H 4.53 0.005 1 249 . 28 G H8 H 7.16 0.005 1 250 . 28 G C1' C 92.4 0.1 1 251 . 28 G C2' C 75.4 0.1 1 252 . 28 G C3' C 73.1 0.1 1 253 . 28 G C8 C 136.4 0.1 1 254 . 29 A H1' H 5.94 0.005 1 255 . 29 A H2 H 7.88 0.005 1 256 . 29 A H2' H 4.46 0.005 1 257 . 29 A H3' H 4.69 0.005 1 258 . 29 A H4' H 4.44 0.005 1 259 . 29 A H5' H 4.11 0.005 1 260 . 29 A H8 H 7.93 0.005 1 261 . 29 A C1' C 91.7 0.1 1 262 . 29 A C2 C 154.9 0.1 1 263 . 29 A C2' C 76.0 0.1 1 264 . 29 A C3' C 74.2 0.1 1 265 . 29 A C4' C 83.2 0.1 1 266 . 29 A C8 C 140.0 0.1 1 267 . 30 G H1' H 5.65 0.005 1 268 . 30 G H2' H 4.64 0.005 1 269 . 30 G H3' H 4.70 0.005 1 270 . 30 G H4' H 4.45 0.005 1 271 . 30 G H5'' H 4.27 0.005 1 272 . 30 G H5' H 4.12 0.005 1 273 . 30 G H8 H 7.66 0.005 1 274 . 30 G C1' C 90.5 0.1 1 275 . 30 G C2' C 74.9 0.1 1 276 . 30 G C4' C 84.6 0.1 1 277 . 30 G C8 C 139.3 0.1 1 278 . 31 G H1' H 5.86 0.005 1 279 . 31 G H2' H 4.81 0.005 1 280 . 31 G H3' H 4.83 0.005 1 281 . 31 G H4' H 4.54 0.005 1 282 . 31 G H8 H 7.90 0.005 1 283 . 31 G C1' C 90.3 0.1 1 284 . 31 G C2' C 75.4 0.1 1 285 . 31 G C4' C 84.9 0.1 1 286 . 31 G C8 C 139.7 0.1 1 287 . 32 C H1' H 5.67 0.005 1 288 . 32 C H5 H 5.60 0.005 1 289 . 32 C H2' H 4.58 0.005 1 290 . 32 C H3' H 4.61 0.005 1 291 . 32 C H4' H 4.51 0.005 1 292 . 32 C H6 H 7.76 0.005 1 293 . 32 C C1' C 93.7 0.1 1 294 . 32 C C5 C 98.6 0.1 1 295 . 32 C C3' C 73.6 0.1 1 296 . 32 C C4' C 82.7 0.1 1 297 . 32 C C6 C 142.0 0.1 1 298 . 33 G H1' H 5.80 0.005 1 299 . 33 G H2' H 4.56 0.005 1 300 . 33 G H3' H 4.64 0.005 1 301 . 33 G H8 H 7.75 0.005 1 302 . 33 G C1' C 93.0 0.1 1 303 . 33 G C8 C 136.7 0.1 1 304 . 33 G H1 H 12.98 0.005 1 305 . 33 G N1 N 148.4 0.1 1 306 . 34 U H1' H 5.62 0.005 1 307 . 34 U H5 H 5.16 0.005 1 308 . 34 U H2' H 4.31 0.005 1 309 . 34 U H6 H 7.82 0.005 1 310 . 34 U C1' C 93.6 0.1 1 311 . 34 U C5 C 102.6 0.1 1 312 . 34 U C2' C 75.5 0.1 1 313 . 34 U C6 C 141.8 0.1 1 314 . 34 U H3 H 14.46 0.005 1 315 . 34 U N3 N 163.6 0.1 1 316 . 35 C H1' H 5.90 0.005 1 317 . 35 C H5 H 5.71 0.005 1 318 . 35 C H2' H 4.02 0.005 1 319 . 35 C H3' H 4.22 0.005 1 320 . 35 C H4' H 4.21 0.005 1 321 . 35 C H5'' H 4.49 0.005 1 322 . 35 C H5' H 4.05 0.005 1 323 . 35 C H6 H 7.78 0.005 1 324 . 35 C C1' C 92.7 0.1 1 325 . 35 C C5 C 98.1 0.1 1 326 . 35 C C2' C 77.5 0.1 1 327 . 35 C C3' C 70.0 0.1 1 328 . 35 C C4' C 83.5 0.1 1 329 . 35 C C6 C 142.3 0.1 1 stop_ save_