data_7246 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone assignments of the RUNT domain of the mouse PEBP2alpha protein ; _BMRB_accession_number 7246 _BMRB_flat_file_name bmr7246.str _Entry_type original _Submission_date 2006-07-26 _Accession_date 2006-07-26 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details ; PEBP2alpha-A also known as: CBFA1, AML3, RUNX2 or OSF-2 ; loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Perez-Alvarado Gabriela C. . 2 Munnerlyn Audrey . . 3 Dyson H. Jane . 4 Grosschedl Rudolf . . 5 Wright Peter E. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 94 "13C chemical shifts" 96 "15N chemical shifts" 94 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2006-08-16 original author . stop_ _Original_release_date 2006-08-16 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Perez-Alvarado, G.C., Munnerlyn, A., Dyson, H.J., Grosschedl, R. and Wright, P.E. (2000) Identification of the regions involved in DNA binding by the mouse PEBP2alpha protein, FEBS Lett. 470, 125-130. ; _Citation_title 'Identification of the regions involved in DNA binding by the mouse PEBP2alpha protein' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 10734220 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Perez-Alvarado Gabriela C. . 2 Munnerlyn Audrey . . 3 Dyson H. Jane . 4 Grosschedl Rudolf . . 5 Wright Peter E. . stop_ _Journal_abbreviation 'FEBS Lett.' _Journal_volume 470 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 125 _Page_last 130 _Year 2000 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'PEBP2alpha DNA binding domain' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'PEBP2alpha DNA binding domain' $RUNT_domain stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state protein _System_paramagnetic no _System_thiol_state 'all free' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_RUNT_domain _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'PEBP2alpha DNA binding domain' _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'transcription factor' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 136 _Mol_residue_sequence ; DNRTMVEIIADHPAELVRTD SPNFLCSVLPSHWRCNKTLP VAFKVVALGEVPDGTVVTVM AGNDENYSAELRNASAVMKN QVARFNDLRFVGRSGRGKSF TLTITVFTNPPQVATYHRAI KVTVDGPREPRRHRQK ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 90 ASP 2 91 ASN 3 92 ARG 4 93 THR 5 94 MET 6 95 VAL 7 96 GLU 8 97 ILE 9 98 ILE 10 99 ALA 11 100 ASP 12 101 HIS 13 102 PRO 14 103 ALA 15 104 GLU 16 105 LEU 17 106 VAL 18 107 ARG 19 108 THR 20 109 ASP 21 110 SER 22 111 PRO 23 112 ASN 24 113 PHE 25 114 LEU 26 115 CYS 27 116 SER 28 117 VAL 29 118 LEU 30 119 PRO 31 120 SER 32 121 HIS 33 122 TRP 34 123 ARG 35 124 CYS 36 125 ASN 37 126 LYS 38 127 THR 39 128 LEU 40 129 PRO 41 130 VAL 42 131 ALA 43 132 PHE 44 133 LYS 45 134 VAL 46 135 VAL 47 136 ALA 48 137 LEU 49 138 GLY 50 139 GLU 51 140 VAL 52 141 PRO 53 142 ASP 54 143 GLY 55 144 THR 56 145 VAL 57 146 VAL 58 147 THR 59 148 VAL 60 149 MET 61 150 ALA 62 151 GLY 63 152 ASN 64 153 ASP 65 154 GLU 66 155 ASN 67 156 TYR 68 157 SER 69 158 ALA 70 159 GLU 71 160 LEU 72 161 ARG 73 162 ASN 74 163 ALA 75 164 SER 76 165 ALA 77 166 VAL 78 167 MET 79 168 LYS 80 169 ASN 81 170 GLN 82 171 VAL 83 172 ALA 84 173 ARG 85 174 PHE 86 175 ASN 87 176 ASP 88 177 LEU 89 178 ARG 90 179 PHE 91 180 VAL 92 181 GLY 93 182 ARG 94 183 SER 95 184 GLY 96 185 ARG 97 186 GLY 98 187 LYS 99 188 SER 100 189 PHE 101 190 THR 102 191 LEU 103 192 THR 104 193 ILE 105 194 THR 106 195 VAL 107 196 PHE 108 197 THR 109 198 ASN 110 199 PRO 111 200 PRO 112 201 GLN 113 202 VAL 114 203 ALA 115 204 THR 116 205 TYR 117 206 HIS 118 207 ARG 119 208 ALA 120 209 ILE 121 210 LYS 122 211 VAL 123 212 THR 124 213 VAL 125 214 ASP 126 215 GLY 127 216 PRO 128 217 ARG 129 218 GLU 130 219 PRO 131 220 ARG 132 221 ARG 133 222 HIS 134 223 ARG 135 224 GLN 136 225 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAA03485 "PEBP2a1 protein [Mus musculus]" 100.00 513 100.00 100.00 6.23e-93 DBJ BAA03486 "PEBP2a2 protein [Mus musculus]" 100.00 306 100.00 100.00 5.40e-96 DBJ BAB40441 "core binding factor alpha 1 [Rattus norvegicus]" 74.26 120 100.00 100.00 1.41e-65 DBJ BAG61865 "unnamed protein product [Homo sapiens]" 67.65 322 97.83 98.91 1.24e-54 DBJ BAK62687 "runt-related transcription factor 2 [Pan troglodytes]" 100.00 256 100.00 100.00 3.22e-96 GB AAA89072 "core-binding factor, runt domain, alpha subunit 1, partial [Homo sapiens]" 100.00 440 100.00 100.00 3.78e-93 GB AAB65158 "core binding factor alpha1 subunit [Homo sapiens]" 100.00 507 100.00 100.00 1.27e-92 GB AAB65159 "core binding factor alpha1 subunit [Homo sapiens]" 100.00 521 100.00 100.00 1.96e-92 GB AAB65409 "Osf2 [Mus musculus]" 100.00 596 100.00 100.00 1.54e-91 GB AAB82419 "PEBP2alphaA major til-1 isoform [Mus musculus]" 100.00 528 100.00 100.00 1.22e-92 REF NP_001015051 "runt-related transcription factor 2 isoform b [Homo sapiens]" 100.00 499 100.00 100.00 8.93e-93 REF NP_001019801 "runt-related transcription factor 2 isoform a [Homo sapiens]" 100.00 521 100.00 100.00 1.96e-92 REF NP_001139392 "runt-related transcription factor 2 isoform 2 [Mus musculus]" 100.00 514 100.00 100.00 6.61e-93 REF NP_001139510 "runt-related transcription factor 2 isoform 1 [Mus musculus]" 100.00 528 100.00 100.00 1.22e-92 REF NP_001258556 "runt-related transcription factor 2 isoform 1 [Mus musculus]" 100.00 528 100.00 100.00 1.22e-92 SP Q08775 "RecName: Full=Runt-related transcription factor 2; AltName: Full=Acute myeloid leukemia 3 protein; AltName: Full=Core-binding f" 100.00 607 100.00 100.00 2.31e-91 SP Q13950 "RecName: Full=Runt-related transcription factor 2; AltName: Full=Acute myeloid leukemia 3 protein; AltName: Full=Core-binding f" 100.00 521 100.00 100.00 1.96e-92 SP Q9Z2J9 "RecName: Full=Runt-related transcription factor 2; AltName: Full=Core-binding factor subunit alpha-1; Short=CBF-alpha-1; AltNam" 74.26 218 100.00 100.00 3.02e-64 TPG DAA16468 "TPA: runt-related transcription factor 2-like isoform 1 [Bos taurus]" 100.00 518 100.00 100.00 2.02e-92 TPG DAA16469 "TPA: runt-related transcription factor 2-like isoform 2 [Bos taurus]" 100.00 496 100.00 100.00 1.01e-92 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $RUNT_domain Mouse 10090 Eukaryota Metazoa Mus musculus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name _Vendor_name $RUNT_domain 'recombinant technology' 'E. coli' Escherichia coli BL21(DE3) plasmid pet21d Novagen stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details ; 2H,15N,13C-PEBP2alpha (residues 90-225). The protein studied had a high level of deuterium labeling (~85%). The 15N and 13C chemical shifts reported have not been corrected for the 2H isotope effects." ; loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RUNT_domain 0.2 mM '[U-2H; U-15N; U-13C]' 'Tris HCl-d8' 20 mM . K2SO4 20 mM . KCl 100 mM . DTT 10 mM . D2O 5 % . stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address NIH . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_2 _Saveframe_category software _Name Felix97 _Version . loop_ _Vendor _Address _Electronic_address 'Molecular Simulations' 'San Diego, CA' . stop_ loop_ _Task processing stop_ _Details . save_ save_software_3 _Saveframe_category software _Name NMRDraw _Version . loop_ _Vendor _Address _Electronic_address NIH . . stop_ loop_ _Task analysis stop_ _Details . save_ save_software_4 _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address OneMoonScientific . . stop_ loop_ _Task analysis stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_600MHz_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_1H15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name 1H15N_HSQC _Sample_label $sample_1 save_ save_1H15N_NOESY_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name 1H15N_NOESY_HSQC _Sample_label $sample_1 save_ save_CT_HNCA_3 _Saveframe_category NMR_applied_experiment _Experiment_name CT_HNCA _Sample_label $sample_1 save_ save_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name HNCACB _Sample_label $sample_1 save_ save_1H15N_HSQC _Saveframe_category NMR_applied_experiment _Experiment_name '1H15N HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_1H15N_NOESY_HSQC _Saveframe_category NMR_applied_experiment _Experiment_name '1H15N NOESY HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_CT_HNCA _Saveframe_category NMR_applied_experiment _Experiment_name 'CT HNCA' _BMRB_pulse_sequence_accession_number . _Details . save_ save_HNCACB _Saveframe_category NMR_applied_experiment _Experiment_name HNCACB _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_conditions_1 _Saveframe_category sample_conditions _Details '20 mM Tris HCl-d8, pH 6.5, 20 mM K2SO4, 100 mM KCl, 10 mM DTT, 5% D2O' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 0.2 pH temperature 295 0.2 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_referencing _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.0 . indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details ; 1H, 13C and 15N backbone chemical shifts of the RUNT domain of PEBP2-alpha A1 residues 90-225 with no corrections for 15N and 13C chemical shifts due to the effects of the 2H isotope. ; loop_ _Software_label $software_4 stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $conditions_1 _Chem_shift_reference_set_label $chemical_shift_referencing _Mol_system_component_name 'PEBP2alpha DNA binding domain' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 90 1 ASP CA C 53.91 0.08 1 2 90 1 ASP CB C 41.32 0.08 1 3 91 2 ASN H H 8.63 0.02 1 4 91 2 ASN CA C 53.09 0.08 1 5 91 2 ASN CB C 38.41 0.08 1 6 91 2 ASN N N 120.23 0.07 1 7 92 3 ARG H H 8.34 0.02 1 8 92 3 ARG CA C 56.29 0.08 1 9 92 3 ARG CB C 29.94 0.08 1 10 92 3 ARG N N 121.2 0.07 1 11 93 4 THR H H 8.25 0.02 1 12 93 4 THR CA C 61.84 0.08 1 13 93 4 THR CB C 69.49 0.08 1 14 93 4 THR N N 114.53 0.07 1 15 94 5 MET H H 8.41 0.02 1 16 94 5 MET CA C 55.72 0.08 1 17 94 5 MET N N 122.15 0.07 1 18 95 6 VAL H H 8.04 0.02 1 19 95 6 VAL CA C 62.74 0.08 1 20 95 6 VAL CB C 31.87 0.08 1 21 95 6 VAL N N 120.24 0.07 1 22 96 7 GLU H H 8.25 0.02 1 23 96 7 GLU CA C 56.50 0.08 1 24 96 7 GLU CB C 29.69 0.08 1 25 96 7 GLU N N 123.69 0.07 1 26 97 8 ILE H H 8.23 0.02 1 27 97 8 ILE CA C 61.31 0.08 1 28 97 8 ILE CB C 37.52 0.08 1 29 97 8 ILE N N 122.45 0.07 1 30 98 9 ILE H H 8.26 0.02 1 31 98 9 ILE CA C 60.50 0.08 1 32 98 9 ILE CB C 37.44 0.08 1 33 98 9 ILE N N 124.37 0.07 1 34 99 10 ALA H H 8.34 0.02 1 35 99 10 ALA CA C 51.99 0.08 1 36 99 10 ALA CB C 18.55 0.08 1 37 99 10 ALA N N 128.02 0.07 1 38 100 11 ASP H H 8.13 0.02 1 39 100 11 ASP CA C 54.11 0.08 1 40 100 11 ASP CB C 40.51 0.08 1 41 100 11 ASP N N 118.69 0.07 1 42 101 12 HIS H H 8.22 0.02 1 43 101 12 HIS CA C 53.87 0.08 1 44 101 12 HIS CB C 28.64 0.08 1 45 101 12 HIS N N 119.19 0.07 1 46 102 13 PRO CB C 31.39 0.08 1 47 103 14 ALA H H 8.51 0.02 1 48 103 14 ALA CA C 52.15 0.08 1 49 103 14 ALA CB C 18.80 0.08 1 50 103 14 ALA N N 123.88 0.07 1 51 104 15 GLU H H 8.31 0.02 1 52 104 15 GLU CA C 56.56 0.08 1 53 104 15 GLU CB C 29.61 0.08 1 54 104 15 GLU N N 119.37 0.07 1 55 105 16 LEU H H 7.96 0.02 1 56 105 16 LEU CA C 52.99 0.08 1 57 105 16 LEU CB C 43.90 0.08 1 58 105 16 LEU N N 120.52 0.07 1 59 106 17 VAL H H 9.49 0.02 1 60 106 17 VAL CA C 59.03 0.08 1 61 106 17 VAL N N 118.12 0.07 1 62 108 19 THR H H 7.93 0.02 1 63 108 19 THR CB C 70.06 0.08 1 64 108 19 THR N N 110.52 0.07 1 65 109 20 ASP H H 8.63 0.02 1 66 109 20 ASP CA C 54.46 0.08 1 67 109 20 ASP CB C 39.38 0.08 1 68 109 20 ASP N N 117.01 0.07 1 69 110 21 SER H H 8.44 0.02 1 70 110 21 SER CA C 53.27 0.08 1 71 110 21 SER N N 115.66 0.07 1 72 112 23 ASN H H 7.86 0.02 1 73 112 23 ASN CA C 52.80 0.08 1 74 112 23 ASN CB C 39.26 0.08 1 75 112 23 ASN N N 112.28 0.07 1 76 113 24 PHE H H 7.39 0.02 1 77 113 24 PHE CA C 57.27 0.08 1 78 113 24 PHE CB C 42.65 0.08 1 79 113 24 PHE N N 114.24 0.07 1 80 114 25 LEU H H 9.35 0.02 1 81 114 25 LEU CA C 52.78 0.08 1 82 114 25 LEU CB C 45.25 0.08 1 83 114 25 LEU N N 123.92 0.07 1 84 116 27 SER H H 7.99 0.02 1 85 116 27 SER N N 114.88 0.07 1 86 117 28 VAL H H 7.62 0.02 1 87 117 28 VAL N N 117.69 0.07 1 88 118 29 LEU H H 8.03 0.02 1 89 118 29 LEU CA C 51.56 0.08 1 90 118 29 LEU N N 128.95 0.07 1 91 121 32 HIS H H 7.63 0.02 1 92 121 32 HIS N N 120.59 0.07 1 93 122 33 TRP H H 8.73 0.02 1 94 122 33 TRP N N 125.26 0.07 1 95 125 36 ASN H H 9.49 0.02 1 96 125 36 ASN N N 117.76 0.07 1 97 126 37 LYS H H 7.64 0.02 1 98 126 37 LYS N N 120.01 0.07 1 99 130 41 VAL H H 6.95 0.02 1 100 130 41 VAL N N 114.10 0.07 1 101 131 42 ALA H H 8.23 0.02 1 102 131 42 ALA CA C 52.17 0.08 1 103 131 42 ALA N N 126.91 0.07 1 104 132 43 PHE H H 8.42 0.02 1 105 132 43 PHE N N 124.48 0.07 1 106 133 44 LYS H H 8.13 0.02 1 107 133 44 LYS N N 125.22 0.07 1 108 134 45 VAL H H 8.43 0.02 1 109 134 45 VAL N N 118.93 0.07 1 110 135 46 VAL H H 9.06 0.02 1 111 135 46 VAL CA C 60.56 0.08 1 112 135 46 VAL N N 127.61 0.07 1 113 136 47 ALA H H 9.23 0.02 1 114 136 47 ALA CA C 50.13 0.08 1 115 136 47 ALA N N 129.05 0.07 1 116 137 48 LEU H H 8.15 0.02 1 117 137 48 LEU CA C 54.38 0.08 1 118 137 48 LEU CB C 40.72 0.08 1 119 137 48 LEU N N 121.16 0.07 1 120 138 49 GLY H H 8.07 0.02 1 121 138 49 GLY CA C 43.15 0.08 1 122 138 49 GLY N N 109.09 0.07 1 123 139 50 GLU H H 8.23 0.02 1 124 139 50 GLU CA C 56.54 0.08 1 125 139 50 GLU CB C 28.32 0.08 1 126 139 50 GLU N N 119.23 0.07 1 127 140 51 VAL H H 8.29 0.02 1 128 140 51 VAL CA C 58.93 0.08 1 129 140 51 VAL CB C 34.55 0.08 1 130 140 51 VAL N N 126.70 0.07 1 131 141 52 PRO CA C 62.44 0.08 1 132 142 53 ASP H H 9.16 0.02 1 133 142 53 ASP CA C 55.09 0.08 1 134 142 53 ASP CB C 39.39 0.08 1 135 142 53 ASP N N 125.65 0.07 1 136 143 54 GLY H H 9.16 0.02 1 137 143 54 GLY CA C 44.34 0.08 1 138 143 54 GLY N N 110.15 0.07 1 139 144 55 THR H H 7.43 0.02 1 140 144 55 THR CA C 64.30 0.08 1 141 144 55 THR N N 117.87 0.07 1 142 145 56 VAL H H 8.91 0.02 1 143 145 56 VAL CA C 63.54 0.08 1 144 145 56 VAL CB C 31.59 0.08 1 145 145 56 VAL N N 130.77 0.07 1 146 146 57 VAL H H 8.76 0.02 1 147 146 57 VAL CB C 34.06 0.08 1 148 146 57 VAL N N 126.82 0.07 1 149 147 58 THR H H 9.43 0.02 1 150 147 58 THR N N 116.22 0.07 1 151 148 59 VAL H H 8.27 0.02 1 152 148 59 VAL N N 117.43 0.07 1 153 149 60 MET H H 8.38 0.02 1 154 149 60 MET N N 122.18 0.07 1 155 150 61 ALA H H 8.95 0.02 1 156 150 61 ALA N N 124.90 0.07 1 157 151 62 GLY H H 8.15 0.02 1 158 151 62 GLY N N 105.11 0.07 1 159 152 63 ASN H H 8.78 0.02 1 160 152 63 ASN N N 117.91 0.07 1 161 153 64 ASP H H 8.47 0.02 1 162 153 64 ASP N N 115.67 0.07 1 163 156 67 TYR H H 8.38 0.02 1 164 156 67 TYR N N 122.50 0.07 1 165 158 69 ALA H H 8.60 0.02 1 166 158 69 ALA N N 128.61 0.07 1 167 160 71 LEU H H 8.75 0.02 1 168 160 71 LEU N N 127.69 0.07 1 169 162 73 ASN H H 9.01 0.02 1 170 162 73 ASN N N 118.22 0.07 1 171 163 74 ALA H H 8.12 0.02 1 172 163 74 ALA N N 119.56 0.07 1 173 164 75 SER H H 7.65 0.02 1 174 164 75 SER CA C 57.05 0.08 1 175 164 75 SER N N 112.15 0.07 1 176 165 76 ALA H H 9.30 0.02 1 177 165 76 ALA CA C 50.25 0.08 1 178 165 76 ALA CB C 20.04 0.08 1 179 165 76 ALA N N 127.52 0.07 1 180 166 77 VAL H H 8.12 0.02 1 181 166 77 VAL CA C 59.84 0.08 1 182 166 77 VAL CB C 33.88 0.08 1 183 166 77 VAL N N 115.59 0.07 1 184 167 78 MET H H 8.95 0.02 1 185 167 78 MET CA C 55.71 0.08 1 186 167 78 MET N N 125.06 0.07 1 187 168 79 LYS H H 8.75 0.02 1 188 168 79 LYS CA C 55.66 0.08 1 189 168 79 LYS CB C 33.70 0.08 1 190 168 79 LYS N N 125.33 0.07 1 191 169 80 ASN H H 10.04 0.02 1 192 169 80 ASN CA C 54.50 0.08 1 193 169 80 ASN CB C 37.21 0.08 1 194 169 80 ASN N N 127.26 0.07 1 195 170 81 GLN H H 9.31 0.02 1 196 170 81 GLN CA C 58.35 0.08 1 197 170 81 GLN CB C 25.78 0.08 1 198 170 81 GLN N N 105.82 0.07 1 199 171 82 VAL H H 7.60 0.02 1 200 171 82 VAL CA C 60.78 0.08 1 201 171 82 VAL CB C 34.67 0.08 1 202 171 82 VAL N N 117.46 0.07 1 203 172 83 ALA H H 9.49 0.02 1 204 172 83 ALA N N 132.00 0.07 1 205 173 84 ARG H H 8.69 0.02 1 206 173 84 ARG N N 123.81 0.07 1 207 174 85 PHE H H 9.24 0.02 1 208 174 85 PHE N N 126.88 0.07 1 209 175 86 ASN H H 9.13 0.02 1 210 175 86 ASN N N 125.59 0.07 1 211 176 87 ASP H H 8.89 0.02 1 212 176 87 ASP N N 120.22 0.07 1 213 177 88 LEU H H 7.83 0.02 1 214 177 88 LEU N N 122.61 0.07 1 215 179 90 PHE H H 9.13 0.02 1 216 179 90 PHE CA C 56.74 0.08 1 217 179 90 PHE N N 121.00 0.07 1 218 182 93 ARG H H 8.13 0.02 1 219 182 93 ARG N N 115.72 0.07 1 220 189 100 PHE H H 9.08 0.02 1 221 189 100 PHE N N 119.98 0.07 1 222 192 103 THR H H 9.11 0.02 1 223 192 103 THR N N 120.79 0.07 1 224 193 104 ILE H H 9.69 0.02 1 225 193 104 ILE N N 132.48 0.07 1 226 194 105 THR H H 8.82 0.02 1 227 194 105 THR CA C 61.82 0.08 1 228 194 105 THR CB C 68.11 0.08 1 229 194 105 THR N N 122.81 0.07 1 230 195 106 VAL H H 9.08 0.02 1 231 195 106 VAL CA C 60.47 0.08 1 232 195 106 VAL CB C 32.07 0.08 1 233 195 106 VAL N N 126.14 0.07 1 234 196 107 PHE H H 9.39 0.02 1 235 196 107 PHE CA C 60.54 0.08 1 236 196 107 PHE CB C 35.33 0.08 1 237 196 107 PHE N N 128.66 0.07 1 238 197 108 THR H H 8.11 0.02 1 239 197 108 THR CA C 62.82 0.08 1 240 197 108 THR CB C 69.98 0.08 1 241 197 108 THR N N 111.15 0.07 1 242 198 109 ASN H H 8.62 0.02 1 243 198 109 ASN CA C 49.36 0.08 1 244 198 109 ASN CB C 39.45 0.08 1 245 198 109 ASN N N 116.60 0.07 1 246 201 112 GLN H H 8.63 0.02 1 247 201 112 GLN CA C 54.15 0.08 1 248 201 112 GLN CB C 32.98 0.08 1 249 201 112 GLN N N 119.97 0.07 1 250 202 113 VAL H H 8.31 0.02 1 251 202 113 VAL CA C 60.07 0.08 1 252 202 113 VAL N N 120.77 0.07 1 253 203 114 ALA H H 9.40 0.02 1 254 203 114 ALA N N 130.63 0.07 1 255 204 115 THR H H 9.17 0.02 1 256 204 115 THR N N 117.71 0.07 1 257 205 116 TYR H H 9.18 0.02 1 258 205 116 TYR N N 128.99 0.07 1 259 211 122 VAL H H 8.37 0.02 1 260 211 122 VAL CA C 61.11 0.08 1 261 211 122 VAL N N 127.20 0.07 1 262 217 128 ARG H H 8.30 0.02 1 263 217 128 ARG N N 123.11 0.07 1 264 218 129 GLU H H 7.98 0.02 1 265 218 129 GLU N N 120.62 0.07 1 266 220 131 ARG H H 8.47 0.02 1 267 220 131 ARG N N 120.98 0.07 1 268 221 132 ARG H H 8.49 0.02 1 269 221 132 ARG CA C 55.20 0.08 1 270 221 132 ARG CB C 29.89 0.08 1 271 221 132 ARG N N 121.11 0.07 1 272 222 133 HIS H H 8.45 0.02 1 273 222 133 HIS CA C 55.46 0.08 1 274 222 133 HIS N N 121.41 0.07 1 275 223 134 ARG H H 8.46 0.02 1 276 223 134 ARG N N 123.60 0.07 1 277 224 135 GLN H H 8.53 0.02 1 278 224 135 GLN CA C 55.58 0.08 1 279 224 135 GLN CB C 28.86 0.08 1 280 224 135 GLN N N 122.46 0.07 1 281 225 136 LYS H H 8.06 0.02 1 282 225 136 LYS CA C 57.56 0.08 1 283 225 136 LYS CB C 32.86 0.08 1 284 225 136 LYS N N 128.04 0.07 1 stop_ save_