data_7391 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Electrostatic contributions to the stability of the GCN4 leucine zipper structure. ; _BMRB_accession_number 7391 _BMRB_flat_file_name bmr7391.str _Entry_type original _Submission_date 2007-06-19 _Accession_date 2007-06-19 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Matousek William M. . 2 Alexandrescu Andrei T. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 62 "13C chemical shifts" 94 "15N chemical shifts" 32 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2009-04-15 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 15316 'native protein' stop_ _Original_release_date 2009-04-15 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Electrostatic contributions to the stability of the GCN4 leucine zipper structure.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17920624 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Matousek William M. . 2 Ciani Barbara . . 3 Fitch Carolyn A. . 4 Garcia-Moreno Bertrand E. . 5 Kammerer Richard A. . 6 Alexandrescu Andrei T. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 374 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 206 _Page_last 219 _Year 2007 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name GCN4 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label GCN4 $GCN4p-wt stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_GCN4p-wt _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common GCN4p-wt _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 33 _Mol_residue_sequence ; GSMKQLEDKVEELLSKNYHL ENEVARLKKLVGE ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 SER 3 MET 4 LYS 5 GLN 6 LEU 7 GLU 8 ASP 9 LYS 10 VAL 11 GLU 12 GLU 13 LEU 14 LEU 15 SER 16 LYS 17 ASN 18 TYR 19 HIS 20 LEU 21 GLU 22 ASN 23 GLU 24 VAL 25 ALA 26 ARG 27 LEU 28 LYS 29 LYS 30 LEU 31 VAL 32 GLY 33 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1396 "transcriptional regulator GCN4" 93.94 61 100.00 100.00 1.90e-11 BMRB 1397 "transcriptional regulator GCN4" 93.94 61 100.00 100.00 1.90e-11 BMRB 1398 "transcriptional regulator GCN4" 93.94 61 100.00 100.00 1.90e-11 BMRB 1399 "transcriptional regulator GCN4" 93.94 61 100.00 100.00 1.90e-11 BMRB 1451 "transcriptional regulator GCN4" 93.94 61 100.00 100.00 1.90e-11 BMRB 1452 "transcriptional regulator GCN4" 93.94 61 100.00 100.00 1.90e-11 BMRB 1453 "transcriptional regulator GCN4" 93.94 61 100.00 100.00 1.90e-11 BMRB 1454 "transcriptional regulator GCN4" 93.94 61 100.00 100.00 1.90e-11 BMRB 15316 GCN4p-wt 100.00 33 100.00 100.00 1.97e-12 BMRB 17511 LZ-GCN4 93.94 36 100.00 100.00 9.07e-11 BMRB 2062 transcriptional_regulator_GCN4-p2N 100.00 35 96.97 96.97 1.13e-11 BMRB 371 "transcriptional regulator GCN4" 93.94 33 100.00 100.00 8.77e-11 PDB 1DGC "The X-Ray Structure Of The Gcn4-Bzip Bound To AtfCREB SITE Dna Shows The Complex Depends On Dna Flexibility" 93.94 62 100.00 100.00 1.98e-11 PDB 1LD4 "Placement Of The Structural Proteins In Sindbis Virus" 93.94 57 100.00 100.00 1.94e-11 PDB 1LLM "Crystal Structure Of A Zif23-Gcn4 Chimera Bound To Dna" 84.85 88 100.00 100.00 8.26e-09 PDB 1NKN "Visualizing An Unstable Coiled Coil: The Crystal Structure Of An N-Terminal Segment Of The Scallop Myosin Rod" 100.00 89 100.00 100.00 2.41e-12 PDB 1YSA "The Gcn4 Basic Region Leucine Zipper Binds Dna As A Dimer Of Uninterrupted Alpha Helices: Crystal Structure Of The Protein-Dna " 93.94 58 100.00 100.00 2.37e-11 PDB 1ZTA "The Solution Structure Of A Leucine-Zipper Motif Peptide" 93.94 35 100.00 100.00 7.80e-11 PDB 2DGC "Gcn4 Basic Domain, Leucine Zipper Complexed With AtfCREB Site Dna" 93.94 63 100.00 100.00 1.96e-11 PDB 2EFR "Crystal Structure Of The C-Terminal Tropomyosin Fragment With N- And C-Terminal Extensions Of The Leucine Zipper At 1.8 Angstro" 84.85 155 100.00 100.00 2.03e-08 PDB 2EFS "Crystal Structure Of The C-Terminal Tropomyosin Fragment With N- And C-Terminal Extensions Of The Leucine Zipper At 2.0 Angstro" 84.85 155 100.00 100.00 2.03e-08 PDB 2OVN "Nmr Structure Of The Gcn4 Trigger Peptide" 51.52 17 100.00 100.00 1.30e-01 PDB 2ZTA "X-Ray Structure Of The Gcn4 Leucine Zipper, A Two-Stranded, Parallel Coiled Coil" 93.94 34 100.00 100.00 8.49e-11 PDB 3BAS "Crystal Structure Of The N-Terminal Region Of The Scallop Myosin Rod, Monoclinic (C2) Form" 100.00 89 100.00 100.00 2.41e-12 PDB 3BAT "Crystal Structure Of The N-Terminal Region Of The Scallop Myosin Rod, Monoclinic (P21) Form" 100.00 89 100.00 100.00 2.41e-12 PDB 3I5C "Crystal Structure Of A Fusion Protein Containing The Leucine Zipper Of Gcn4 And The Ggdef Domain Of Wspr From Pseudomonas Aerug" 87.88 206 100.00 100.00 3.32e-09 PDB 4DMD "Gcn4 Leucine Zipper Domain In A Dimeric Oligomerization State" 93.94 34 100.00 100.00 8.49e-11 PDB 4DME "Gcn4 Leucine Zipper Domain In A Trimeric Oligomerization State" 93.94 35 100.00 100.00 8.59e-11 PDB 4LPZ "Arnt Transcription Factor/coactivator Complex" 51.52 44 100.00 100.00 5.68e-02 DBJ GAA22815 "K7_Gcn4p [Saccharomyces cerevisiae Kyokai no. 7]" 93.94 281 100.00 100.00 5.86e-11 EMBL CAE52206 "Gcn4p [Saccharomyces cerevisiae]" 93.94 281 100.00 100.00 5.92e-11 EMBL CAE52207 "Gcn4p [Saccharomyces cerevisiae]" 93.94 281 100.00 100.00 5.92e-11 EMBL CAE52208 "Gcn4p [Saccharomyces cerevisiae]" 93.94 281 100.00 100.00 5.92e-11 EMBL CAE52209 "Gcn4p [Saccharomyces cerevisiae]" 93.94 281 100.00 100.00 5.92e-11 EMBL CAE52210 "Gcn4p [Saccharomyces cerevisiae]" 93.94 281 100.00 100.00 5.86e-11 GB AAA34640 "GCN4 protein [Saccharomyces cerevisiae]" 93.94 281 100.00 100.00 5.98e-11 GB AAB64486 "Transcriptional activator of amino acid biosynthetic genes [Saccharomyces cerevisiae]" 93.94 281 100.00 100.00 5.98e-11 GB AAC33186 "hinge-region and leucine-zipper with N-terminal PelB-leader and C-terminal hexahistidine tag [synthetic construct]" 93.94 84 100.00 100.00 3.86e-11 GB AAD37724 "PelB-IgG kappa light chain fusion protein [Cloning vector pCLZip]" 93.94 192 100.00 100.00 7.61e-11 GB AAK07887 "cI-GCN4 repressor fusion protein [Cloning vector pJH370]" 93.94 166 100.00 100.00 1.31e-10 REF NP_010907 "Gcn4p [Saccharomyces cerevisiae S288c]" 93.94 281 100.00 100.00 5.98e-11 SP P03069 "RecName: Full=General control protein GCN4; AltName: Full=Amino acid biosynthesis regulatory protein [Saccharomyces cerevisiae " 93.94 281 100.00 100.00 5.98e-11 TPG DAA07643 "TPA: amino acid starvation-responsive transcription factor GCN4 [Saccharomyces cerevisiae S288c]" 93.94 281 100.00 100.00 5.98e-11 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $GCN4p-wt 'baker's yeast' 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $GCN4p-wt 'recombinant technology' . Escherichia coli BL21 NA stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_Denatured_GCN4p-wt_1 _Saveframe_category sample _Sample_type solution _Details '1.8 mM 13C/15N GCN4p-wt, 6 M urea, 150 mM NaCl, 10 mM phosphate buffer, 10% D2O, pH 2.3, 25C' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $GCN4p-wt 1.8 mM '[U-13C; U-15N]' urea 6 M 'natural abundance' 'sodium chloride' 150 mM 'natural abundance' 'sodium phosphate' 10 mM 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_Felix _Saveframe_category software _Name FELIX _Version 2000.1 loop_ _Vendor _Address _Electronic_address 'Accelrys Software Inc.' . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details Cryoprobe save_ ############################# # NMR applied experiments # ############################# save_3D_HNCACB_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $Denatured_GCN4p-wt_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $Denatured_GCN4p-wt_1 save_ save_3D_HN(CO)CA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $Denatured_GCN4p-wt_1 save_ save_3D_C(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D C(CO)NH' _Sample_label $Denatured_GCN4p-wt_1 save_ save_3D_H(CCO)NH_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D H(CCO)NH' _Sample_label $Denatured_GCN4p-wt_1 save_ save_3D_HNCO_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $Denatured_GCN4p-wt_1 save_ ####################### # Sample conditions # ####################### save_Denatured_GCN4p-wt_cond _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 2.3 . pH temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 $entry_citation $entry_citation DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 $entry_citation $entry_citation DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_denatured_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $Felix stop_ loop_ _Experiment_label '3D HNCACB' '3D HNCA' '3D HN(CO)CA' '3D C(CO)NH' '3D H(CCO)NH' '3D HNCO' stop_ loop_ _Sample_label $Denatured_GCN4p-wt_1 stop_ _Sample_conditions_label $Denatured_GCN4p-wt_cond _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name GCN4 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 0 1 GLY C C 170.4 0.20 1 2 0 1 GLY CA C 43.7 0.20 1 3 1 2 SER H H 8.73 0.01 1 4 1 2 SER HA H 4.58 0.03 1 5 1 2 SER C C 174.7 0.20 1 6 1 2 SER CA C 58.5 0.20 1 7 1 2 SER CB C 64.1 0.20 1 8 1 2 SER N N 114.4 0.10 1 9 2 3 MET H H 8.62 0.01 1 10 2 3 MET HA H 4.48 0.03 1 11 2 3 MET C C 176.4 0.20 1 12 2 3 MET CA C 55.9 0.20 1 13 2 3 MET CB C 33.0 0.20 1 14 2 3 MET N N 121.2 0.10 1 15 3 4 LYS H H 8.44 0.01 1 16 3 4 LYS HA H 4.27 0.03 1 17 3 4 LYS C C 176.6 0.20 1 18 3 4 LYS CA C 56.9 0.20 1 19 3 4 LYS CB C 33.1 0.20 1 20 3 4 LYS N N 121.8 0.10 1 21 4 5 GLN H H 8.47 0.01 1 22 4 5 GLN HA H 4.27 0.03 1 23 4 5 GLN C C 176.1 0.20 1 24 4 5 GLN CA C 56.1 0.20 1 25 4 5 GLN CB C 29.4 0.20 1 26 4 5 GLN N N 120.3 0.10 1 27 5 6 LEU H H 8.40 0.01 1 28 5 6 LEU HA H 4.37 0.03 1 29 5 6 LEU C C 177.5 0.20 1 30 5 6 LEU CA C 55.4 0.20 1 31 5 6 LEU CB C 42.3 0.20 1 32 5 6 LEU N N 122.7 0.10 1 33 6 7 GLU H H 8.43 0.01 1 34 6 7 GLU HA H 4.37 0.03 1 35 6 7 GLU C C 176.0 0.20 1 36 6 7 GLU CA C 55.8 0.20 1 37 6 7 GLU CB C 28.7 0.20 1 38 6 7 GLU N N 119.3 0.10 1 39 7 8 ASP H H 8.49 0.01 1 40 7 8 ASP HA H 4.69 0.03 1 41 7 8 ASP C C 175.2 0.20 1 42 7 8 ASP CA C 53.2 0.20 1 43 7 8 ASP CB C 38.1 0.20 1 44 7 8 ASP N N 119.0 0.10 1 45 8 9 LYS H H 8.34 0.01 1 46 8 9 LYS HA H 4.27 0.03 1 47 8 9 LYS C C 176.7 0.20 1 48 8 9 LYS CA C 56.9 0.20 1 49 8 9 LYS CB C 33.0 0.20 1 50 8 9 LYS N N 120.6 0.10 1 51 9 10 VAL H H 8.17 0.01 1 52 9 10 VAL HA H 4.27 0.03 1 53 9 10 VAL C C 176.4 0.20 1 54 9 10 VAL CA C 62.8 0.20 1 55 9 10 VAL CB C 32.8 0.20 1 56 9 10 VAL N N 119.6 0.10 1 57 10 11 GLU H H 8.37 0.01 1 58 10 11 GLU HA H 4.27 0.03 1 59 10 11 GLU C C 176.1 0.20 1 60 10 11 GLU CA C 56.2 0.20 1 61 10 11 GLU CB C 28.9 0.20 1 62 10 11 GLU N N 122.1 0.10 1 63 11 12 GLU H H 8.39 0.01 1 64 11 12 GLU HA H 4.27 0.03 1 65 11 12 GLU C C 176.0 0.20 1 66 11 12 GLU CA C 56.1 0.20 1 67 11 12 GLU CB C 28.8 0.20 1 68 11 12 GLU N N 120.3 0.10 1 69 12 13 LEU H H 8.32 0.01 1 70 12 13 LEU HA H 4.34 0.03 1 71 12 13 LEU C C 177.3 0.20 1 72 12 13 LEU CA C 55.4 0.20 1 73 12 13 LEU CB C 42.3 0.20 1 74 12 13 LEU N N 122.1 0.10 1 75 13 14 LEU H H 8.31 0.01 1 76 13 14 LEU HA H 4.38 0.03 1 77 13 14 LEU C C 177.6 0.20 1 78 13 14 LEU CA C 55.3 0.20 1 79 13 14 LEU CB C 42.3 0.20 1 80 13 14 LEU N N 121.5 0.10 1 81 14 15 SER H H 8.29 0.01 1 82 14 15 SER HA H 4.38 0.03 1 83 14 15 SER C C 174.9 0.20 1 84 14 15 SER CA C 58.6 0.20 1 85 14 15 SER CB C 63.9 0.20 1 86 14 15 SER N N 115.0 0.10 1 87 15 16 LYS H H 8.38 0.01 1 88 15 16 LYS HA H 4.27 0.03 1 89 15 16 LYS C C 176.2 0.20 1 90 15 16 LYS CA C 56.8 0.20 1 91 15 16 LYS CB C 33.0 0.20 1 92 15 16 LYS N N 121.5 0.10 1 93 16 17 ASN H H 8.36 0.01 1 94 16 17 ASN HA H 4.69 0.03 1 95 16 17 ASN C C 175.1 0.20 1 96 16 17 ASN CA C 53.2 0.20 1 97 16 17 ASN CB C 38.8 0.20 1 98 16 17 ASN N N 117.5 0.10 1 99 17 18 TYR H H 8.12 0.01 1 100 17 18 TYR HA H 4.48 0.03 1 101 17 18 TYR C C 175.7 0.20 1 102 17 18 TYR CA C 58.5 0.20 1 103 17 18 TYR CB C 38.7 0.20 1 104 17 18 TYR N N 119.6 0.10 1 105 18 19 HIS H H 8.50 0.01 1 106 18 19 HIS HA H 4.48 0.03 1 107 18 19 HIS C C 174.2 0.20 1 108 18 19 HIS CA C 55.5 0.20 1 109 18 19 HIS CB C 28.9 0.20 1 110 18 19 HIS N N 118.7 0.10 1 111 19 20 LEU H H 8.26 0.01 1 112 19 20 LEU HA H 4.27 0.03 1 113 19 20 LEU C C 177.4 0.20 1 114 19 20 LEU CA C 55.5 0.20 1 115 19 20 LEU CB C 42.3 0.20 1 116 19 20 LEU N N 121.9 0.10 1 117 20 21 GLU H H 8.46 0.01 1 118 20 21 GLU CB C 28.9 0.20 1 119 20 21 GLU N N 119.6 0.10 1 120 21 22 ASN H H 8.50 0.01 1 121 21 22 ASN HA H 4.48 0.03 1 122 21 22 ASN C C 175.3 0.20 1 123 21 22 ASN CA C 53.4 0.20 1 124 21 22 ASN CB C 38.3 0.20 1 125 21 22 ASN N N 118.7 0.10 1 126 22 23 GLU H H 8.35 0.01 1 127 22 23 GLU HA H 4.37 0.03 1 128 22 23 GLU C C 176.7 0.20 1 129 22 23 GLU CA C 56.2 0.20 1 130 22 23 GLU CB C 28.8 0.20 1 131 22 23 GLU N N 119.3 0.10 1 132 23 24 VAL H H 8.15 0.01 1 133 23 24 VAL HA H 4.06 0.03 1 134 23 24 VAL C C 176.0 0.20 1 135 23 24 VAL CA C 62.7 0.20 1 136 23 24 VAL CB C 32.7 0.20 1 137 23 24 VAL N N 119.6 0.10 1 138 24 25 ALA H H 8.33 0.01 1 139 24 25 ALA C C 177.8 0.20 1 140 24 25 ALA CA C 52.8 0.20 1 141 24 25 ALA CB C 19.1 0.20 1 142 24 25 ALA N N 127.1 0.10 1 143 25 26 ARG H H 8.29 0.01 1 144 25 26 ARG C C 176.4 0.20 1 145 25 26 ARG CA C 56.4 0.20 1 146 25 26 ARG CB C 30.8 0.20 1 147 25 26 ARG N N 119.0 0.10 1 148 26 27 LEU H H 8.23 0.01 1 149 26 27 LEU HA H 4.27 0.03 1 150 26 27 LEU C C 177.4 0.20 1 151 26 27 LEU CA C 55.4 0.20 1 152 26 27 LEU CB C 42.4 0.20 1 153 26 27 LEU N N 121.8 0.10 1 154 27 28 LYS H H 8.46 0.01 1 155 27 28 LYS HA H 4.27 0.03 1 156 27 28 LYS C C 176.5 0.20 1 157 27 28 LYS CA C 56.7 0.20 1 158 27 28 LYS CB C 33.1 0.20 1 159 27 28 LYS N N 121.5 0.10 1 160 28 29 LYS H H 8.40 0.01 1 161 28 29 LYS HA H 4.27 0.03 1 162 28 29 LYS C C 176.4 0.20 1 163 28 29 LYS CA C 56.6 0.20 1 164 28 29 LYS N N 121.8 0.10 1 165 29 30 LEU H H 8.39 0.01 1 166 29 30 LEU HA H 4.38 0.03 1 167 29 30 LEU C C 177.3 0.20 1 168 29 30 LEU CA C 55.2 0.20 1 169 29 30 LEU CB C 42.3 0.20 1 170 29 30 LEU N N 123.0 0.10 1 171 30 31 VAL H H 8.26 0.01 1 172 30 31 VAL HA H 4.17 0.03 1 173 30 31 VAL C C 176.6 0.20 1 174 30 31 VAL CA C 62.6 0.20 1 175 30 31 VAL CB C 32.9 0.20 1 176 30 31 VAL N N 119.9 0.10 1 177 31 32 GLY H H 8.49 0.01 1 178 31 32 GLY HA2 H 3.96 0.03 2 179 31 32 GLY HA3 H 3.96 0.03 2 180 31 32 GLY C C 173.9 0.20 1 181 31 32 GLY CA C 45.3 0.20 1 182 31 32 GLY N N 111.0 0.10 1 183 32 33 GLU H H 8.21 0.01 1 184 32 33 GLU HA H 4.27 0.03 1 185 32 33 GLU C C 178.5 0.20 1 186 32 33 GLU CA C 55.3 0.20 1 187 32 33 GLU CB C 28.7 0.20 1 188 32 33 GLU N N 119.0 0.10 1 stop_ save_