data_16755 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 16755 _Entry.Title ; Complement assembly of two fragments of the streptococcal protein G B1 domain in aqueous solution ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2010-03-03 _Entry.Accession_date 2010-03-03 _Entry.Last_release_date 2010-03-24 _Entry.Original_release_date 2010-03-24 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Naohiro Kobayashi . . . 16755 2 Shinya Honda . . . 16755 3 Hirofumi Yoshii . . . 16755 4 Hatsuho Uedaira . . . 16755 5 Eisuke Munekata . . . 16755 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID binding_constants 1 16755 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'binding constants' 1 16755 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2010-03-24 2010-03-03 original author . 16755 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 16755 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 7789539 _Citation.Full_citation . _Citation.Title 'Complement assembly of two fragments of the streptococcal protein G B1 domain in aqueous solution' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'FEBS Lett.' _Citation.Journal_name_full . _Citation.Journal_volume 366 _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 99 _Citation.Page_last 103 _Citation.Year 1995 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Naohiro Kobayashi . . . 16755 1 2 Shinya Honda . . . 16755 1 3 Hirofumi Yoshii . . . 16755 1 4 Hatsuho Uedaira . . . 16755 1 5 Eisuke Munekata . . . 16755 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 16755 _Assembly.ID 1 _Assembly.Name 'N40 C16 complex' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 N40 1 $N40 A . yes native no no . . . 16755 1 2 C16 2 $C16 B . yes native no no . . . 16755 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_N40 _Entity.Sf_category entity _Entity.Sf_framecode N40 _Entity.Entry_ID 16755 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name N40 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; DTYKLILNGKTLKGETTTEA VDAATAEKVFKQYANDNGVD ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 40 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 15156 . GB1 . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 2 no BMRB 15380 . GB1 . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 3 no BMRB 16053 . entity . . . . . 95.00 141 97.37 97.37 2.64e-13 . . . . 16755 1 4 no BMRB 16444 . SC35 . . . . . 95.00 158 100.00 100.00 5.16e-16 . . . . 16755 1 5 no BMRB 16627 . Protein_GB1_(2Q6I) . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 6 no BMRB 16873 . GB1 . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 7 no BMRB 16882 . "Ubiquitin-Binding Motif" . . . . . 95.00 108 100.00 100.00 3.54e-16 . . . . 16755 1 8 no BMRB 16958 . ZCCHC9 . . . . . 95.00 164 100.00 100.00 3.86e-16 . . . . 16755 1 9 no BMRB 17810 . entity . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 10 no BMRB 18397 . GB1 . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 11 no BMRB 19394 . GB1-UBM1 . . . . . 95.00 106 100.00 100.00 3.18e-16 . . . . 16755 1 12 no BMRB 26630 . Protein_G_Domain_Beta-1_Wild_Type . . . . . 97.50 64 100.00 100.00 2.19e-17 . . . . 16755 1 13 no PDB 1GB1 . "A Novel, Highly Stable Fold Of The Immunoglobulin Binding Domain Of Streptococcal Protein G" . . . . . 97.50 56 100.00 100.00 4.81e-17 . . . . 16755 1 14 no PDB 1IBX . "Nmr Structure Of Dff40 And Dff45 N-Terminal Domain Complex" . . . . . 95.00 145 100.00 100.00 5.43e-16 . . . . 16755 1 15 no PDB 1PGA . "Two Crystal Structures Of The B1 Immunoglobulin-Binding Domain Of Streptococcal Protein G And Comparison With Nmr" . . . . . 97.50 56 100.00 100.00 4.81e-17 . . . . 16755 1 16 no PDB 1PGB . "Two Crystal Structures Of The B1 Immunoglobulin-Binding Domain Of Streptoccocal Protein G And Comparison With Nmr" . . . . . 97.50 56 100.00 100.00 4.81e-17 . . . . 16755 1 17 no PDB 1PN5 . "Nmr Structure Of The Nalp1 Pyrin Domain (Pyd)" . . . . . 95.00 159 100.00 100.00 2.98e-16 . . . . 16755 1 18 no PDB 2CWB . "Solution Structure Of The Ubiquitin-Associated Domain Of Human Bmsc-Ubp And Its Complex With Ubiquitin" . . . . . 95.00 108 97.37 97.37 2.05e-15 . . . . 16755 1 19 no PDB 2DEN . "Solution Structure Of The Ubiquitin-Associated Domain Of Human Bmsc-Ubp And Its Complex With Ubiquitin" . . . . . 95.00 108 97.37 97.37 2.05e-15 . . . . 16755 1 20 no PDB 2GB1 . "A Novel, Highly Stable Fold Of The Immunoglobulin Binding Domain Of Streptococcal Protein G" . . . . . 97.50 56 100.00 100.00 4.81e-17 . . . . 16755 1 21 no PDB 2GI9 . "Backbone Conformational Constraints In A Microcrystalline U- 15n-Labeled Protein By 3d Dipolar-Shift Solid-State Nmr Spectrosco" . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 22 no PDB 2I2Y . "Solution Structure Of The Rrm Of Srp20 Bound To The Rna Cauc" . . . . . 95.00 150 100.00 100.00 9.49e-16 . . . . 16755 1 23 no PDB 2I38 . "Solution Structure Of The Rrm Of Srp20" . . . . . 95.00 150 100.00 100.00 9.49e-16 . . . . 16755 1 24 no PDB 2J52 . "Solution Structure Of Gb1 Domain Protein G And Low And High Pressure." . . . . . 97.50 56 100.00 100.00 4.56e-17 . . . . 16755 1 25 no PDB 2J53 . "Solution Structure Of Gb1 Domain Protein G And Low And High Pressure" . . . . . 97.50 56 100.00 100.00 4.56e-17 . . . . 16755 1 26 no PDB 2JSV . "Dipole Tensor-Based Refinement For Atomic-Resolution Structure Determination Of A Nanocrystalline Protein By Solid-State Nmr Sp" . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 27 no PDB 2JU6 . "Solid-State Protein Structure Determination With Proton- Detected Triple Resonance 3d Magic-Angle Spinning Nmr Spectroscopy" . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 28 no PDB 2K0P . "Determination Of A Protein Structure In The Solid State From Nmr Chemical Shifts" . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 29 no PDB 2KBT . "Attachment Of An Nmr-Invisible Solubility Enhancement Tag (Inset) Using A Sortase-Mediated Protein Ligation Method" . . . . . 97.50 142 100.00 100.00 1.09e-16 . . . . 16755 1 30 no PDB 2KHU . "Solution Structure Of The Ubiquitin-Binding Motif Of Human Polymerase Iota" . . . . . 95.00 108 100.00 100.00 3.54e-16 . . . . 16755 1 31 no PDB 2KHW . "Solution Structure Of The Human Polymerase Iota Ubm2- Ubiquitin Complex" . . . . . 95.00 108 100.00 100.00 3.54e-16 . . . . 16755 1 32 no PDB 2KLK . "Solution Structure Of Gb1 A34f Mutant With Rdc And Saxs" . . . . . 95.00 56 97.37 97.37 1.49e-15 . . . . 16755 1 33 no PDB 2KN4 . "The Structure Of The Rrm Domain Of Sc35" . . . . . 95.00 158 100.00 100.00 5.16e-16 . . . . 16755 1 34 no PDB 2KQ4 . "Atomic Resolution Protein Structure Determination By Three- Dimensional Transferred Echo Double Resonance Solid-State Nuclear M" . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 35 no PDB 2KWD . "Supramolecular Protein Structure Determination By Site-Speci Range Intermolecular Solid State Nmr Spectroscopy" . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 36 no PDB 2LGI . "Atomic Resolution Protein Structures Using Nmr Chemical Shift Tensors" . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 37 no PDB 2MBB . "Solution Structure Of The Human Polymerase Iota Ubm1-ubiquitin Complex" . . . . . 95.00 106 100.00 100.00 3.18e-16 . . . . 16755 1 38 no PDB 2PLP . "Ultra High Resolution Backbone Conformation Of Protein Gb1 From Residual Dipolar Couplings Alone" . . . . . 97.50 54 100.00 100.00 5.60e-17 . . . . 16755 1 39 no PDB 2QMT . "Crystal Polymorphism Of Protein Gb1 Examined By Solid-State Nmr And X-Ray Diffraction" . . . . . 95.00 56 100.00 100.00 1.69e-16 . . . . 16755 1 40 no PDB 2RMM . "Solution Structure Of Gb1 A34f Mutant" . . . . . 95.00 56 97.37 97.37 1.49e-15 . . . . 16755 1 41 no PDB 2RPV . "Solution Structure Of Gb1 With Lbt Probe" . . . . . 95.00 75 97.37 97.37 5.61e-15 . . . . 16755 1 42 no PDB 3GB1 . "Structures Of B1 Domain Of Streptococcal Protein G" . . . . . 97.50 56 100.00 100.00 4.81e-17 . . . . 16755 1 43 no PDB 3MP9 . "Structure Of Streptococcal Protein G B1 Domain At Ph 3.0" . . . . . 100.00 64 100.00 100.00 3.66e-18 . . . . 16755 1 44 no PDB 3UI3 . "Structural And Biochemical Characterization Of Hp0315 From Helicobacter Pylori As A Vapd Protein With An Endoribonuclease Activ" . . . . . 95.00 160 100.00 100.00 9.36e-16 . . . . 16755 1 45 no PDB 4KGS . "Backbone Modifications In The Protein Gb1 Loops: Beta-3-val21, Beta-3- Asp40" . . . . . 97.50 57 97.44 97.44 3.84e-16 . . . . 16755 1 46 no PDB 4Q0C . "3.1 A Resolution Crystal Structure Of The B. Pertussis Bvgs Periplasmic Domain" . . . . . 95.00 584 97.37 97.37 1.04e-13 . . . . 16755 1 47 no GB AAA26599 . "BBM3XM [Staphylococcus xylosus]" . . . . . 65.00 573 100.00 100.00 4.41e-06 . . . . 16755 1 48 no GB AAA26600 . "BBXM [Staphylococcus xylosus]" . . . . . 65.00 492 100.00 100.00 4.65e-06 . . . . 16755 1 49 no GB AAY41168 . "protein G/SspDnaE fusion protein [Expression vector pJJDuet30]" . . . . . 95.00 201 100.00 100.00 9.88e-16 . . . . 16755 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . ASP . 16755 1 2 . THR . 16755 1 3 . TYR . 16755 1 4 . LYS . 16755 1 5 . LEU . 16755 1 6 . ILE . 16755 1 7 . LEU . 16755 1 8 . ASN . 16755 1 9 . GLY . 16755 1 10 . LYS . 16755 1 11 . THR . 16755 1 12 . LEU . 16755 1 13 . LYS . 16755 1 14 . GLY . 16755 1 15 . GLU . 16755 1 16 . THR . 16755 1 17 . THR . 16755 1 18 . THR . 16755 1 19 . GLU . 16755 1 20 . ALA . 16755 1 21 . VAL . 16755 1 22 . ASP . 16755 1 23 . ALA . 16755 1 24 . ALA . 16755 1 25 . THR . 16755 1 26 . ALA . 16755 1 27 . GLU . 16755 1 28 . LYS . 16755 1 29 . VAL . 16755 1 30 . PHE . 16755 1 31 . LYS . 16755 1 32 . GLN . 16755 1 33 . TYR . 16755 1 34 . ALA . 16755 1 35 . ASN . 16755 1 36 . ASP . 16755 1 37 . ASN . 16755 1 38 . GLY . 16755 1 39 . VAL . 16755 1 40 . ASP . 16755 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . ASP 1 1 16755 1 . THR 2 2 16755 1 . TYR 3 3 16755 1 . LYS 4 4 16755 1 . LEU 5 5 16755 1 . ILE 6 6 16755 1 . LEU 7 7 16755 1 . ASN 8 8 16755 1 . GLY 9 9 16755 1 . LYS 10 10 16755 1 . THR 11 11 16755 1 . LEU 12 12 16755 1 . LYS 13 13 16755 1 . GLY 14 14 16755 1 . GLU 15 15 16755 1 . THR 16 16 16755 1 . THR 17 17 16755 1 . THR 18 18 16755 1 . GLU 19 19 16755 1 . ALA 20 20 16755 1 . VAL 21 21 16755 1 . ASP 22 22 16755 1 . ALA 23 23 16755 1 . ALA 24 24 16755 1 . THR 25 25 16755 1 . ALA 26 26 16755 1 . GLU 27 27 16755 1 . LYS 28 28 16755 1 . VAL 29 29 16755 1 . PHE 30 30 16755 1 . LYS 31 31 16755 1 . GLN 32 32 16755 1 . TYR 33 33 16755 1 . ALA 34 34 16755 1 . ASN 35 35 16755 1 . ASP 36 36 16755 1 . ASN 37 37 16755 1 . GLY 38 38 16755 1 . VAL 39 39 16755 1 . ASP 40 40 16755 1 stop_ save_ save_C16 _Entity.Sf_category entity _Entity.Sf_framecode C16 _Entity.Entry_ID 16755 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name C16 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code GEWTYDDATKTFTVTE _Entity.Target_identifier . _Entity.Polymer_author_defined_seq 41,G _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 16 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-01-30 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 15156 . GB1 . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 2 no BMRB 15380 . GB1 . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 3 no BMRB 16053 . entity . . . . . 100.00 141 100.00 100.00 4.75e-01 . . . . 16755 2 4 no BMRB 1639 . "IgG Fc region-binding protein" . . . . . 100.00 64 100.00 100.00 2.40e-01 . . . . 16755 2 5 no BMRB 16444 . SC35 . . . . . 100.00 158 100.00 100.00 2.98e-01 . . . . 16755 2 6 no BMRB 16627 . Protein_GB1_(2Q6I) . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 7 no BMRB 16670 . NALP1_PYD . . . . . 100.00 159 100.00 100.00 2.53e-01 . . . . 16755 2 8 no BMRB 16873 . GB1 . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 9 no BMRB 16882 . "Ubiquitin-Binding Motif" . . . . . 100.00 108 100.00 100.00 2.00e-01 . . . . 16755 2 10 no BMRB 16958 . ZCCHC9 . . . . . 100.00 164 100.00 100.00 2.41e-01 . . . . 16755 2 11 no BMRB 17810 . entity . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 12 no BMRB 18397 . GB1 . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 13 no BMRB 19394 . GB1-UBM1 . . . . . 100.00 106 100.00 100.00 1.35e-01 . . . . 16755 2 14 no BMRB 5151 . protein_G . . . . . 100.00 57 100.00 100.00 2.48e-01 . . . . 16755 2 15 no BMRB 5569 . GB1 . . . . . 100.00 56 100.00 100.00 2.35e-01 . . . . 16755 2 16 no BMRB 5654 . pG . . . . . 100.00 56 100.00 100.00 2.40e-01 . . . . 16755 2 17 no BMRB 5875 . GB1_monomer . . . . . 100.00 56 100.00 100.00 2.22e-01 . . . . 16755 2 18 no BMRB 6280 . dsRBD12 . . . . . 100.00 294 100.00 100.00 4.87e-02 . . . . 16755 2 19 no BMRB 6988 . HGB1-UBA . . . . . 100.00 108 100.00 100.00 1.29e-01 . . . . 16755 2 20 no BMRB 7280 . GB1 . . . . . 100.00 58 100.00 100.00 2.32e-01 . . . . 16755 2 21 no PDB 1EM7 . "Helix Variant Of The B1 Domain From Streptococcal Protein G" . . . . . 100.00 56 100.00 100.00 2.76e-01 . . . . 16755 2 22 no PDB 1FCC . "Crystal Structure Of The C2 Fragment Of Streptococcal Protein G In Complex With The Fc Domain Of Human Igg" . . . . . 100.00 56 100.00 100.00 2.60e-01 . . . . 16755 2 23 no PDB 1FD6 . "Delta0: A Computationally Designed Core Variant Of The B1 Domain Of Streptococcal Protein G" . . . . . 100.00 57 100.00 100.00 2.48e-01 . . . . 16755 2 24 no PDB 1GB1 . "A Novel, Highly Stable Fold Of The Immunoglobulin Binding Domain Of Streptococcal Protein G" . . . . . 100.00 56 100.00 100.00 2.35e-01 . . . . 16755 2 25 no PDB 1GB4 . "Hyperthermophilic Variant Of The B1 Domain From Streptococcal Protein G, Nmr, 47 Structures" . . . . . 100.00 57 100.00 100.00 1.97e-01 . . . . 16755 2 26 no PDB 1IBX . "Nmr Structure Of Dff40 And Dff45 N-Terminal Domain Complex" . . . . . 100.00 145 100.00 100.00 2.63e-01 . . . . 16755 2 27 no PDB 1MPE . "Ensemble Of 20 Structures Of The Tetrameric Mutant Of The B1 Domain Of Streptococcal Protein G" . . . . . 100.00 56 100.00 100.00 2.40e-01 . . . . 16755 2 28 no PDB 1MVK . "X-ray Structure Of The Tetrameric Mutant Of The B1 Domain Of Streptococcal Protein G" . . . . . 100.00 56 100.00 100.00 2.40e-01 . . . . 16755 2 29 no PDB 1PGA . "Two Crystal Structures Of The B1 Immunoglobulin-Binding Domain Of Streptococcal Protein G And Comparison With Nmr" . . . . . 100.00 56 100.00 100.00 2.35e-01 . . . . 16755 2 30 no PDB 1PGB . "Two Crystal Structures Of The B1 Immunoglobulin-Binding Domain Of Streptoccocal Protein G And Comparison With Nmr" . . . . . 100.00 56 100.00 100.00 2.35e-01 . . . . 16755 2 31 no PDB 1PN5 . "Nmr Structure Of The Nalp1 Pyrin Domain (Pyd)" . . . . . 100.00 159 100.00 100.00 2.53e-01 . . . . 16755 2 32 no PDB 1Q10 . "Ensemble Of 40 Structures Of The Dimeric Mutant Of The B1 Domain Of Streptococcal Protein G" . . . . . 100.00 56 100.00 100.00 2.22e-01 . . . . 16755 2 33 no PDB 1QKZ . "Fab Fragment (Mn14c11.6) In Complex With A Peptide Antigen Derived From Neisseria Meningitidis P1.7 Serosubtype Antigen And Dom" . . . . . 100.00 64 100.00 100.00 2.26e-01 . . . . 16755 2 34 no PDB 1UWX . "P1.2 Serosubtype Antigen Derived From N. Meningitidis Pora In Complex With Fab Fragment" . . . . . 100.00 63 100.00 100.00 2.37e-01 . . . . 16755 2 35 no PDB 2CWB . "Solution Structure Of The Ubiquitin-Associated Domain Of Human Bmsc-Ubp And Its Complex With Ubiquitin" . . . . . 100.00 108 100.00 100.00 1.29e-01 . . . . 16755 2 36 no PDB 2DEN . "Solution Structure Of The Ubiquitin-Associated Domain Of Human Bmsc-Ubp And Its Complex With Ubiquitin" . . . . . 100.00 108 100.00 100.00 1.29e-01 . . . . 16755 2 37 no PDB 2GB1 . "A Novel, Highly Stable Fold Of The Immunoglobulin Binding Domain Of Streptococcal Protein G" . . . . . 100.00 56 100.00 100.00 2.35e-01 . . . . 16755 2 38 no PDB 2GI9 . "Backbone Conformational Constraints In A Microcrystalline U- 15n-Labeled Protein By 3d Dipolar-Shift Solid-State Nmr Spectrosco" . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 39 no PDB 2I2Y . "Solution Structure Of The Rrm Of Srp20 Bound To The Rna Cauc" . . . . . 100.00 150 100.00 100.00 3.13e-01 . . . . 16755 2 40 no PDB 2I38 . "Solution Structure Of The Rrm Of Srp20" . . . . . 100.00 150 100.00 100.00 3.23e-01 . . . . 16755 2 41 no PDB 2IGG . "Determination Of The Solution Structures Of Domains Ii And Iii Of Protein G From Streptococcus By 1h Nmr" . . . . . 100.00 64 100.00 100.00 2.40e-01 . . . . 16755 2 42 no PDB 2JSV . "Dipole Tensor-Based Refinement For Atomic-Resolution Structure Determination Of A Nanocrystalline Protein By Solid-State Nmr Sp" . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 43 no PDB 2JU6 . "Solid-State Protein Structure Determination With Proton- Detected Triple Resonance 3d Magic-Angle Spinning Nmr Spectroscopy" . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 44 no PDB 2K0P . "Determination Of A Protein Structure In The Solid State From Nmr Chemical Shifts" . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 45 no PDB 2KBT . "Attachment Of An Nmr-Invisible Solubility Enhancement Tag (Inset) Using A Sortase-Mediated Protein Ligation Method" . . . . . 100.00 142 100.00 100.00 4.88e-01 . . . . 16755 2 46 no PDB 2KHU . "Solution Structure Of The Ubiquitin-Binding Motif Of Human Polymerase Iota" . . . . . 100.00 108 100.00 100.00 2.00e-01 . . . . 16755 2 47 no PDB 2KHW . "Solution Structure Of The Human Polymerase Iota Ubm2- Ubiquitin Complex" . . . . . 100.00 108 100.00 100.00 2.00e-01 . . . . 16755 2 48 no PDB 2KLK . "Solution Structure Of Gb1 A34f Mutant With Rdc And Saxs" . . . . . 100.00 56 100.00 100.00 2.40e-01 . . . . 16755 2 49 no PDB 2KN4 . "The Structure Of The Rrm Domain Of Sc35" . . . . . 100.00 158 100.00 100.00 2.98e-01 . . . . 16755 2 50 no PDB 2KQ4 . "Atomic Resolution Protein Structure Determination By Three- Dimensional Transferred Echo Double Resonance Solid-State Nuclear M" . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 51 no PDB 2KWD . "Supramolecular Protein Structure Determination By Site-Speci Range Intermolecular Solid State Nmr Spectroscopy" . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 52 no PDB 2LGI . "Atomic Resolution Protein Structures Using Nmr Chemical Shift Tensors" . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 53 no PDB 2MBB . "Solution Structure Of The Human Polymerase Iota Ubm1-ubiquitin Complex" . . . . . 100.00 106 100.00 100.00 1.35e-01 . . . . 16755 2 54 no PDB 2ON8 . "Gbeta1 Stabilization By In Vitro Evolution And Computational Design" . . . . . 100.00 56 100.00 100.00 1.68e-01 . . . . 16755 2 55 no PDB 2ONQ . "Gbeta1 Stabilization By In Vitro Evolution And Computational Design" . . . . . 100.00 56 100.00 100.00 1.87e-01 . . . . 16755 2 56 no PDB 2PLP . "Ultra High Resolution Backbone Conformation Of Protein Gb1 From Residual Dipolar Couplings Alone" . . . . . 93.75 54 100.00 100.00 1.10e+00 . . . . 16755 2 57 no PDB 2QMT . "Crystal Polymorphism Of Protein Gb1 Examined By Solid-State Nmr And X-Ray Diffraction" . . . . . 100.00 56 100.00 100.00 2.17e-01 . . . . 16755 2 58 no PDB 2RMM . "Solution Structure Of Gb1 A34f Mutant" . . . . . 100.00 56 100.00 100.00 2.40e-01 . . . . 16755 2 59 no PDB 2RPV . "Solution Structure Of Gb1 With Lbt Probe" . . . . . 100.00 75 100.00 100.00 2.53e-01 . . . . 16755 2 60 no PDB 3FIL . "Structural And Energetic Determinants For Hyperstable Variants Of Gb1 Obtained From In-Vitro Evolution" . . . . . 100.00 56 100.00 100.00 1.73e-01 . . . . 16755 2 61 no PDB 3GB1 . "Structures Of B1 Domain Of Streptococcal Protein G" . . . . . 100.00 56 100.00 100.00 2.35e-01 . . . . 16755 2 62 no PDB 3MP9 . "Structure Of Streptococcal Protein G B1 Domain At Ph 3.0" . . . . . 100.00 64 100.00 100.00 1.70e-01 . . . . 16755 2 63 no PDB 3U2S . "Crystal Structure Of Pg9 Fab In Complex With V1v2 Region From Hiv-1 Strain Zm109" . . . . . 100.00 124 100.00 100.00 1.37e-01 . . . . 16755 2 64 no PDB 3U4E . "Crystal Structure Of Pg9 Fab In Complex With V1v2 Region From Hiv-1 Strain Cap45" . . . . . 100.00 124 100.00 100.00 1.52e-01 . . . . 16755 2 65 no PDB 3UI3 . "Structural And Biochemical Characterization Of Hp0315 From Helicobacter Pylori As A Vapd Protein With An Endoribonuclease Activ" . . . . . 100.00 160 100.00 100.00 3.69e-01 . . . . 16755 2 66 no PDB 3V3X . "Nitroxide Spin Labels In Protein Gb1: N8/k28 Double Mutant" . . . . . 100.00 56 100.00 100.00 2.40e-01 . . . . 16755 2 67 no PDB 4DQO . "Crystal Structure Of Pg16 Fab In Complex With V1v2 Region From Hiv-1 Strain Zm109" . . . . . 100.00 124 100.00 100.00 1.37e-01 . . . . 16755 2 68 no PDB 4KGR . "Backbone Modifications In The Protein Gb1 Helix: Beta-3-ala24, Beta-3- Lys28, Beta-3-lys31, Beta-3-asn35" . . . . . 100.00 57 100.00 100.00 3.21e-01 . . . . 16755 2 69 no PDB 4KGS . "Backbone Modifications In The Protein Gb1 Loops: Beta-3-val21, Beta-3- Asp40" . . . . . 100.00 57 100.00 100.00 2.63e-01 . . . . 16755 2 70 no PDB 4OZA . "Backbone Modifications In The Protein Gb1 Helix: Beta-3-ala24, Beta-3- Lys28, Beta-3-gln32, Beta-3-asp36" . . . . . 100.00 57 100.00 100.00 2.96e-01 . . . . 16755 2 71 no PDB 4OZB . "Backbone Modifications In The Protein Gb1 Helix: Beta-acpc24, Beta-3- Lys28, Beta-3-lys31, Beta-acpc35" . . . . . 100.00 57 100.00 100.00 3.21e-01 . . . . 16755 2 72 no PDB 4OZC . "Backbone Modifications In The Protein Gb1 Helix And Loops: Beta- Acpc21, Beta-acpc24, Beta-3-lys28, Beta-3-lys31, Beta-acpc35, " . . . . . 100.00 57 100.00 100.00 3.30e-01 . . . . 16755 2 73 no EMBL CAA37410 . "Protein G' [Streptococcus sp. 'group G']" . . . . . 100.00 185 100.00 100.00 9.14e-01 . . . . 16755 2 74 no GB AAA03280 . "protein LG, partial [Finegoldia magna]" . . . . . 100.00 455 100.00 100.00 1.07e+00 . . . . 16755 2 75 no GB AHW57457 . "GB1-CMTI-III [synthetic construct]" . . . . . 100.00 107 100.00 100.00 1.09e-01 . . . . 16755 2 76 no PIR A45063 . "immunoglobulin-binding protein LG - Peptostreptococcus magnus [Finegoldia magna]" . . . . . 100.00 455 100.00 100.00 1.07e+00 . . . . 16755 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . GLY . 16755 2 2 . GLU . 16755 2 3 . TRP . 16755 2 4 . THR . 16755 2 5 . TYR . 16755 2 6 . ASP . 16755 2 7 . ASP . 16755 2 8 . ALA . 16755 2 9 . THR . 16755 2 10 . LYS . 16755 2 11 . THR . 16755 2 12 . PHE . 16755 2 13 . THR . 16755 2 14 . VAL . 16755 2 15 . THR . 16755 2 16 . GLU . 16755 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 16755 2 . GLU 2 2 16755 2 . TRP 3 3 16755 2 . THR 4 4 16755 2 . TYR 5 5 16755 2 . ASP 6 6 16755 2 . ASP 7 7 16755 2 . ALA 8 8 16755 2 . THR 9 9 16755 2 . LYS 10 10 16755 2 . THR 11 11 16755 2 . PHE 12 12 16755 2 . THR 13 13 16755 2 . VAL 14 14 16755 2 . THR 15 15 16755 2 . GLU 16 16 16755 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 16755 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 2 $C16 . 1301 organism . Streptococcus Streptococcus . . Bacteria . Streptococcus . . . . . . . . . . . . . . . . . . . . . . 16755 1 2 1 $N40 . 1301 organism . Streptococcus Streptococcus . . Bacteria . Streptococcus . . . . . . . . . . . . . . . . . . . . . . 16755 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 16755 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $N40 . 'chemical synthesis' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16755 1 2 2 $C16 . 'chemical synthesis' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16755 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 16755 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 N40 'natural abundance' . . 1 $N40 . . 1.8 . . mM . . . . 16755 1 2 C16 'natural abundance' . . 2 $C16 . . 1.8 . . mM . . . . 16755 1 3 'phosphate buffer' 'natural abundance' . . . . . . 50 . . mM . . . . 16755 1 4 H2O 'natural abundance' . . . . . . 90 . . % . . . . 16755 1 5 D2O 'natural abundance' . . . . . . 10 . . % . . . . 16755 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 16755 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 5.5 . pH 16755 1 pressure 1 . atm 16755 1 temperature 298 . K 16755 1 stop_ save_ ############################ # Computer software used # ############################ save_MOLSCRIPT _Software.Sf_category software _Software.Sf_framecode MOLSCRIPT _Software.Entry_ID 16755 _Software.ID 1 _Software.Name MOLSCRIPT _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Kraulis, 1991' . . 16755 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID visualization 16755 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 16755 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model AM _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 500 save_ save_spectrometer_2 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_2 _NMR_spectrometer.Entry_ID 16755 _NMR_spectrometer.ID 2 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model AMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 500 save_ save_spectrometer_3 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_3 _NMR_spectrometer.Entry_ID 16755 _NMR_spectrometer.ID 3 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model AMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 16755 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker AM . 500 . . . 16755 1 2 spectrometer_2 Bruker AMX . 500 . . . 16755 1 3 spectrometer_3 Bruker AMX . 600 . . . 16755 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 16755 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D DQF-COSY' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 16755 1 2 '2D HOHAHA' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 16755 1 3 '2D ROESY' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 16755 1 4 '2D 1H-1H NOESY' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 16755 1 stop_ save_ save_binding_data _Binding_value_list.Sf_category binding_data _Binding_value_list.Sf_framecode binding_data _Binding_value_list.Entry_ID 16755 _Binding_value_list.ID 1 _Binding_value_list.Sample_condition_list_ID 1 _Binding_value_list.Sample_condition_list_label $sample_conditions_1 _Binding_value_list.Details . _Binding_value_list.Text_data_format . _Binding_value_list.Text_data . loop_ _Binding_experiment.Experiment_ID _Binding_experiment.Experiment_name _Binding_experiment.Sample_ID _Binding_experiment.Sample_label _Binding_experiment.Sample_state _Binding_experiment.Entry_ID _Binding_experiment.Binding_value_list_ID 1 '2D DQF-COSY' 1 $sample_1 isotropic 16755 1 2 '2D HOHAHA' 2 $sample_1 isotropic 16755 1 3 '2D ROESY' 3 $sample_1 isotropic 16755 1 4 '2D 1H-1H NOESY' 4 $sample_1 isotropic 16755 1 stop_ loop_ _Binding_software.Software_ID _Binding_software.Software_label _Binding_software.Method_ID _Binding_software.Method_label _Binding_software.Entry_ID _Binding_software.Binding_value_list_ID 1 $MOLSCRIPT . . 16755 1 stop_ loop_ _Binding_result.ID _Binding_result.Experiment_ID _Binding_result.Assembly_ID _Binding_result.Atm_obs_assembly_atom_ID _Binding_result.Atm_obs_entity_assembly_ID _Binding_result.Atm_obs_entity_ID _Binding_result.Atm_obs_comp_index_ID _Binding_result.Atm_obs_seq_ID _Binding_result.Atm_obs_comp_ID _Binding_result.Atm_obs_atom_ID _Binding_result.Atm_obs_atom_type _Binding_result.Atm_obs_atom_isotope_number _Binding_result.Resonance_ID _Binding_result.Atm_obs_auth_entity_assembly_ID _Binding_result.Atm_obs_auth_seq_ID _Binding_result.Atm_obs_auth_comp_ID _Binding_result.Atm_obs_auth_atom_ID _Binding_result.Expt_observed_param _Binding_result.Val_type _Binding_result.Val _Binding_result.Val_err _Binding_result.Val_units _Binding_result.Entry_ID _Binding_result.Binding_value_list_ID 1 4 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kd 9 . uM 16755 1 stop_ loop_ _Binding_partners.Binding_result_ID _Binding_partners.Assembly_ID _Binding_partners.Entity_assembly_ID _Binding_partners.Entity_assembly_name _Binding_partners.Entity_ID _Binding_partners.Entity_label _Binding_partners.Entry_ID _Binding_partners.Binding_value_list_ID 1 1 1 N40 1 $N40 16755 1 1 1 2 C16 2 $C16 16755 1 stop_ save_