data_17059 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 17059 _Entry.Title ; Identification of a novel ubiquitin binding site of STAM1 VHS domain by NMR spectroscopy ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2010-07-13 _Entry.Accession_date 2010-07-13 _Entry.Last_release_date 2010-09-08 _Entry.Original_release_date 2010-09-08 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Yoon-Hun Hong . . . 17059 2 Hee-Chul Ahn . . . 17059 3 Jongsoo Lim . . . 17059 4 Hong-Man Kim . . . 17059 5 Hye-Young Ji . . . 17059 6 Seunga Lee . . . 17059 7 Ji-Hun Kim . . . 17059 8 Eun Park . Y. . 17059 9 Hyun Song . K. . 17059 10 Bong-Jin Lee . . . 17059 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID binding_constants 1 17059 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'binding constants' 4 17059 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2010-09-08 2010-07-13 original author . 17059 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 17059 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID . _Citation.Full_citation . _Citation.Title 'Identification of a novel ubiquitin binding site of STAM1 VHS domain by NMR spectroscopy' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'FEBS Lett.' _Citation.Journal_name_full . _Citation.Journal_volume 583 _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 287 _Citation.Page_last 292 _Citation.Year 2009 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Yoon-Hun Hong . . . 17059 1 2 Hee-Chul Ahn . . . 17059 1 3 Jongsoo Lim . . . 17059 1 4 Hong-Man Kim . . . 17059 1 5 Hye-Young Ji . . . 17059 1 6 Seunga Lee . . . 17059 1 7 Ji-Hun Kim . . . 17059 1 8 Eun Park . Y. . 17059 1 9 Hyun Song . K. . 17059 1 10 Bong-Jin Lee . . . 17059 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 17059 _Assembly.ID 1 _Assembly.Name 'STAM1/ubiquitin complex' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 STAM1 1 $STAM1 A . yes native no no . . . 17059 1 2 ubiquitin 2 $ubiquitin B . yes native no no . . . 17059 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_STAM1 _Entity.Sf_category entity _Entity.Sf_framecode STAM1 _Entity.Entry_ID 17059 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name STAM1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; PLFATNPFDQDVEKATSEMN TAEDWGLILDICDKVGQSRT GPKDCLRSIMRRVNHKDPHV AMQALTLLGACVSNCGKIFH LEVCSRDFASEVSNVLNKGH PKVCEKLKALMVEWTDEFKN DPQLSLISAMIKNLKEQGVT FPAIGSQAAEQAKASPALVA KDPGTVANKKEEEDLAKA ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 178 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 16763 . VHS . . . . . 77.53 138 100.00 100.00 1.60e-97 . . . . 17059 1 2 no BMRB 17065 . Tandem_ubiquitin_binding_domains_of_STAM1 . . . . . 100.00 191 100.00 100.00 1.27e-127 . . . . 17059 1 3 no PDB 3LDZ . "Crystal Structure Of Human Stam1 Vhs Domain In Complex With Ubiquitin" . . . . . 78.65 140 100.00 100.00 2.88e-99 . . . . 17059 1 4 no DBJ BAE26170 . "unnamed protein product [Mus musculus]" . . . . . 100.00 553 98.88 99.44 5.34e-124 . . . . 17059 1 5 no DBJ BAE28168 . "unnamed protein product [Mus musculus]" . . . . . 100.00 462 98.88 99.44 3.15e-124 . . . . 17059 1 6 no DBJ BAE34187 . "unnamed protein product [Mus musculus]" . . . . . 100.00 548 98.88 99.44 2.41e-124 . . . . 17059 1 7 no DBJ BAE37536 . "unnamed protein product [Mus musculus]" . . . . . 100.00 548 98.88 99.44 2.41e-124 . . . . 17059 1 8 no DBJ BAE40949 . "unnamed protein product [Mus musculus]" . . . . . 100.00 548 98.88 99.44 2.41e-124 . . . . 17059 1 9 no GB AAC50734 . "STAM [Homo sapiens]" . . . . . 100.00 540 100.00 100.00 3.01e-125 . . . . 17059 1 10 no GB AAC52840 . "STAM [Mus musculus]" . . . . . 100.00 548 98.88 99.44 2.41e-124 . . . . 17059 1 11 no GB AAH30586 . "STAM protein [Homo sapiens]" . . . . . 100.00 403 100.00 100.00 2.19e-125 . . . . 17059 1 12 no GB AAH44666 . "Signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 [Mus musculus]" . . . . . 100.00 548 98.88 99.44 2.41e-124 . . . . 17059 1 13 no GB AAH55326 . "Signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 [Mus musculus]" . . . . . 100.00 548 98.88 99.44 2.41e-124 . . . . 17059 1 14 no REF NP_001070310 . "signal transducing adapter molecule 1 [Bos taurus]" . . . . . 100.00 534 99.44 99.44 4.43e-125 . . . . 17059 1 15 no REF NP_001102591 . "signal transducing adapter molecule 1 [Rattus norvegicus]" . . . . . 100.00 547 98.88 99.44 5.04e-124 . . . . 17059 1 16 no REF NP_003464 . "signal transducing adapter molecule 1 [Homo sapiens]" . . . . . 100.00 540 100.00 100.00 3.01e-125 . . . . 17059 1 17 no REF NP_035614 . "signal transducing adapter molecule 1 [Mus musculus]" . . . . . 100.00 548 98.88 99.44 2.41e-124 . . . . 17059 1 18 no REF XP_001094352 . "PREDICTED: signal transducing adapter molecule 1 [Macaca mulatta]" . . . . . 72.47 490 99.22 99.22 2.62e-84 . . . . 17059 1 19 no SP P70297 . "RecName: Full=Signal transducing adapter molecule 1; Short=STAM-1" . . . . . 100.00 548 98.88 99.44 2.41e-124 . . . . 17059 1 20 no SP Q92783 . "RecName: Full=Signal transducing adapter molecule 1; Short=STAM-1" . . . . . 100.00 540 100.00 100.00 3.01e-125 . . . . 17059 1 21 no TPG DAA23567 . "TPA: signal transducing adaptor molecule 1 [Bos taurus]" . . . . . 100.00 534 99.44 99.44 4.43e-125 . . . . 17059 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . PRO . 17059 1 2 . LEU . 17059 1 3 . PHE . 17059 1 4 . ALA . 17059 1 5 . THR . 17059 1 6 . ASN . 17059 1 7 . PRO . 17059 1 8 . PHE . 17059 1 9 . ASP . 17059 1 10 . GLN . 17059 1 11 . ASP . 17059 1 12 . VAL . 17059 1 13 . GLU . 17059 1 14 . LYS . 17059 1 15 . ALA . 17059 1 16 . THR . 17059 1 17 . SER . 17059 1 18 . GLU . 17059 1 19 . MET . 17059 1 20 . ASN . 17059 1 21 . THR . 17059 1 22 . ALA . 17059 1 23 . GLU . 17059 1 24 . ASP . 17059 1 25 . TRP . 17059 1 26 . GLY . 17059 1 27 . LEU . 17059 1 28 . ILE . 17059 1 29 . LEU . 17059 1 30 . ASP . 17059 1 31 . ILE . 17059 1 32 . CYS . 17059 1 33 . ASP . 17059 1 34 . LYS . 17059 1 35 . VAL . 17059 1 36 . GLY . 17059 1 37 . GLN . 17059 1 38 . SER . 17059 1 39 . ARG . 17059 1 40 . THR . 17059 1 41 . GLY . 17059 1 42 . PRO . 17059 1 43 . LYS . 17059 1 44 . ASP . 17059 1 45 . CYS . 17059 1 46 . LEU . 17059 1 47 . ARG . 17059 1 48 . SER . 17059 1 49 . ILE . 17059 1 50 . MET . 17059 1 51 . ARG . 17059 1 52 . ARG . 17059 1 53 . VAL . 17059 1 54 . ASN . 17059 1 55 . HIS . 17059 1 56 . LYS . 17059 1 57 . ASP . 17059 1 58 . PRO . 17059 1 59 . HIS . 17059 1 60 . VAL . 17059 1 61 . ALA . 17059 1 62 . MET . 17059 1 63 . GLN . 17059 1 64 . ALA . 17059 1 65 . LEU . 17059 1 66 . THR . 17059 1 67 . LEU . 17059 1 68 . LEU . 17059 1 69 . GLY . 17059 1 70 . ALA . 17059 1 71 . CYS . 17059 1 72 . VAL . 17059 1 73 . SER . 17059 1 74 . ASN . 17059 1 75 . CYS . 17059 1 76 . GLY . 17059 1 77 . LYS . 17059 1 78 . ILE . 17059 1 79 . PHE . 17059 1 80 . HIS . 17059 1 81 . LEU . 17059 1 82 . GLU . 17059 1 83 . VAL . 17059 1 84 . CYS . 17059 1 85 . SER . 17059 1 86 . ARG . 17059 1 87 . ASP . 17059 1 88 . PHE . 17059 1 89 . ALA . 17059 1 90 . SER . 17059 1 91 . GLU . 17059 1 92 . VAL . 17059 1 93 . SER . 17059 1 94 . ASN . 17059 1 95 . VAL . 17059 1 96 . LEU . 17059 1 97 . ASN . 17059 1 98 . LYS . 17059 1 99 . GLY . 17059 1 100 . HIS . 17059 1 101 . PRO . 17059 1 102 . LYS . 17059 1 103 . VAL . 17059 1 104 . CYS . 17059 1 105 . GLU . 17059 1 106 . LYS . 17059 1 107 . LEU . 17059 1 108 . LYS . 17059 1 109 . ALA . 17059 1 110 . LEU . 17059 1 111 . MET . 17059 1 112 . VAL . 17059 1 113 . GLU . 17059 1 114 . TRP . 17059 1 115 . THR . 17059 1 116 . ASP . 17059 1 117 . GLU . 17059 1 118 . PHE . 17059 1 119 . LYS . 17059 1 120 . ASN . 17059 1 121 . ASP . 17059 1 122 . PRO . 17059 1 123 . GLN . 17059 1 124 . LEU . 17059 1 125 . SER . 17059 1 126 . LEU . 17059 1 127 . ILE . 17059 1 128 . SER . 17059 1 129 . ALA . 17059 1 130 . MET . 17059 1 131 . ILE . 17059 1 132 . LYS . 17059 1 133 . ASN . 17059 1 134 . LEU . 17059 1 135 . LYS . 17059 1 136 . GLU . 17059 1 137 . GLN . 17059 1 138 . GLY . 17059 1 139 . VAL . 17059 1 140 . THR . 17059 1 141 . PHE . 17059 1 142 . PRO . 17059 1 143 . ALA . 17059 1 144 . ILE . 17059 1 145 . GLY . 17059 1 146 . SER . 17059 1 147 . GLN . 17059 1 148 . ALA . 17059 1 149 . ALA . 17059 1 150 . GLU . 17059 1 151 . GLN . 17059 1 152 . ALA . 17059 1 153 . LYS . 17059 1 154 . ALA . 17059 1 155 . SER . 17059 1 156 . PRO . 17059 1 157 . ALA . 17059 1 158 . LEU . 17059 1 159 . VAL . 17059 1 160 . ALA . 17059 1 161 . LYS . 17059 1 162 . ASP . 17059 1 163 . PRO . 17059 1 164 . GLY . 17059 1 165 . THR . 17059 1 166 . VAL . 17059 1 167 . ALA . 17059 1 168 . ASN . 17059 1 169 . LYS . 17059 1 170 . LYS . 17059 1 171 . GLU . 17059 1 172 . GLU . 17059 1 173 . GLU . 17059 1 174 . ASP . 17059 1 175 . LEU . 17059 1 176 . ALA . 17059 1 177 . LYS . 17059 1 178 . ALA . 17059 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . PRO 1 1 17059 1 . LEU 2 2 17059 1 . PHE 3 3 17059 1 . ALA 4 4 17059 1 . THR 5 5 17059 1 . ASN 6 6 17059 1 . PRO 7 7 17059 1 . PHE 8 8 17059 1 . ASP 9 9 17059 1 . GLN 10 10 17059 1 . ASP 11 11 17059 1 . VAL 12 12 17059 1 . GLU 13 13 17059 1 . LYS 14 14 17059 1 . ALA 15 15 17059 1 . THR 16 16 17059 1 . SER 17 17 17059 1 . GLU 18 18 17059 1 . MET 19 19 17059 1 . ASN 20 20 17059 1 . THR 21 21 17059 1 . ALA 22 22 17059 1 . GLU 23 23 17059 1 . ASP 24 24 17059 1 . TRP 25 25 17059 1 . GLY 26 26 17059 1 . LEU 27 27 17059 1 . ILE 28 28 17059 1 . LEU 29 29 17059 1 . ASP 30 30 17059 1 . ILE 31 31 17059 1 . CYS 32 32 17059 1 . ASP 33 33 17059 1 . LYS 34 34 17059 1 . VAL 35 35 17059 1 . GLY 36 36 17059 1 . GLN 37 37 17059 1 . SER 38 38 17059 1 . ARG 39 39 17059 1 . THR 40 40 17059 1 . GLY 41 41 17059 1 . PRO 42 42 17059 1 . LYS 43 43 17059 1 . ASP 44 44 17059 1 . CYS 45 45 17059 1 . LEU 46 46 17059 1 . ARG 47 47 17059 1 . SER 48 48 17059 1 . ILE 49 49 17059 1 . MET 50 50 17059 1 . ARG 51 51 17059 1 . ARG 52 52 17059 1 . VAL 53 53 17059 1 . ASN 54 54 17059 1 . HIS 55 55 17059 1 . LYS 56 56 17059 1 . ASP 57 57 17059 1 . PRO 58 58 17059 1 . HIS 59 59 17059 1 . VAL 60 60 17059 1 . ALA 61 61 17059 1 . MET 62 62 17059 1 . GLN 63 63 17059 1 . ALA 64 64 17059 1 . LEU 65 65 17059 1 . THR 66 66 17059 1 . LEU 67 67 17059 1 . LEU 68 68 17059 1 . GLY 69 69 17059 1 . ALA 70 70 17059 1 . CYS 71 71 17059 1 . VAL 72 72 17059 1 . SER 73 73 17059 1 . ASN 74 74 17059 1 . CYS 75 75 17059 1 . GLY 76 76 17059 1 . LYS 77 77 17059 1 . ILE 78 78 17059 1 . PHE 79 79 17059 1 . HIS 80 80 17059 1 . LEU 81 81 17059 1 . GLU 82 82 17059 1 . VAL 83 83 17059 1 . CYS 84 84 17059 1 . SER 85 85 17059 1 . ARG 86 86 17059 1 . ASP 87 87 17059 1 . PHE 88 88 17059 1 . ALA 89 89 17059 1 . SER 90 90 17059 1 . GLU 91 91 17059 1 . VAL 92 92 17059 1 . SER 93 93 17059 1 . ASN 94 94 17059 1 . VAL 95 95 17059 1 . LEU 96 96 17059 1 . ASN 97 97 17059 1 . LYS 98 98 17059 1 . GLY 99 99 17059 1 . HIS 100 100 17059 1 . PRO 101 101 17059 1 . LYS 102 102 17059 1 . VAL 103 103 17059 1 . CYS 104 104 17059 1 . GLU 105 105 17059 1 . LYS 106 106 17059 1 . LEU 107 107 17059 1 . LYS 108 108 17059 1 . ALA 109 109 17059 1 . LEU 110 110 17059 1 . MET 111 111 17059 1 . VAL 112 112 17059 1 . GLU 113 113 17059 1 . TRP 114 114 17059 1 . THR 115 115 17059 1 . ASP 116 116 17059 1 . GLU 117 117 17059 1 . PHE 118 118 17059 1 . LYS 119 119 17059 1 . ASN 120 120 17059 1 . ASP 121 121 17059 1 . PRO 122 122 17059 1 . GLN 123 123 17059 1 . LEU 124 124 17059 1 . SER 125 125 17059 1 . LEU 126 126 17059 1 . ILE 127 127 17059 1 . SER 128 128 17059 1 . ALA 129 129 17059 1 . MET 130 130 17059 1 . ILE 131 131 17059 1 . LYS 132 132 17059 1 . ASN 133 133 17059 1 . LEU 134 134 17059 1 . LYS 135 135 17059 1 . GLU 136 136 17059 1 . GLN 137 137 17059 1 . GLY 138 138 17059 1 . VAL 139 139 17059 1 . THR 140 140 17059 1 . PHE 141 141 17059 1 . PRO 142 142 17059 1 . ALA 143 143 17059 1 . ILE 144 144 17059 1 . GLY 145 145 17059 1 . SER 146 146 17059 1 . GLN 147 147 17059 1 . ALA 148 148 17059 1 . ALA 149 149 17059 1 . GLU 150 150 17059 1 . GLN 151 151 17059 1 . ALA 152 152 17059 1 . LYS 153 153 17059 1 . ALA 154 154 17059 1 . SER 155 155 17059 1 . PRO 156 156 17059 1 . ALA 157 157 17059 1 . LEU 158 158 17059 1 . VAL 159 159 17059 1 . ALA 160 160 17059 1 . LYS 161 161 17059 1 . ASP 162 162 17059 1 . PRO 163 163 17059 1 . GLY 164 164 17059 1 . THR 165 165 17059 1 . VAL 166 166 17059 1 . ALA 167 167 17059 1 . ASN 168 168 17059 1 . LYS 169 169 17059 1 . LYS 170 170 17059 1 . GLU 171 171 17059 1 . GLU 172 172 17059 1 . GLU 173 173 17059 1 . ASP 174 174 17059 1 . LEU 175 175 17059 1 . ALA 176 176 17059 1 . LYS 177 177 17059 1 . ALA 178 178 17059 1 stop_ save_ save_ubiquitin _Entity.Sf_category entity _Entity.Sf_framecode ubiquitin _Entity.Entry_ID 17059 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name ubiquitin _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGGAKKR KKKSYTTPKKNKHKRKKVKL AVLKYYKVDENGKISRLRRE CPSDECGAGVFMASHFDRHY CGKCCLTYCFNKPEDK ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 156 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-01-30 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no PDB 3J3A . "Structure Of The Human 40s Ribosomal Proteins" . . . . . 51.28 80 100.00 100.00 8.80e-50 . . . . 17059 2 2 no PDB 4CXC . "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 3 no PDB 4KZX . "Rabbit 40s Ribosomal Subunit In Complex With Eif1." . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 4 no PDB 4KZY . "Rabbit 40s Ribosomal Subunit In Complex With Eif1 And Eif1a." . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 5 no PDB 4KZZ . "Rabbit 40s Ribosomal Subunit In Complex With Mrna, Initiator Trna And Eif1a" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 6 no PDB 4UQ4 . "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 7 no PDB 4UQ5 . "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 8 no PDB 4W23 . "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Small Ribosomal Subunit)" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 9 no PDB 4W28 . "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Small Ribosomal Subunit)" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 10 no DBJ BAA04129 . "polyubiquitin [Rattus norvegicus]" . . . . . 50.00 810 97.44 98.72 5.84e-41 . . . . 17059 2 11 no DBJ BAA09096 . "TI-225 [Mus musculus]" . . . . . 50.00 126 97.44 98.72 2.25e-45 . . . . 17059 2 12 no DBJ BAA11843 . "ubiquitin extention protein [Cavia porcellus]" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 13 no DBJ BAB27296 . "unnamed protein product [Mus musculus]" . . . . . 50.00 354 97.44 98.72 9.22e-43 . . . . 17059 2 14 no DBJ BAB31357 . "unnamed protein product [Mus musculus]" . . . . . 100.00 156 99.36 99.36 9.11e-109 . . . . 17059 2 15 no EMBL CAA44911 . "ubiquitin [Homo sapiens]" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 16 no EMBL CAA57432 . "fusion protein: ubiquitin (bases 43_513); ribosomal protein S27a (bases 217_532) [Rattus norvegicus]" . . . . . 100.00 156 99.36 99.36 9.11e-109 . . . . 17059 2 17 no EMBL CAG12343 . "unnamed protein product, partial [Tetraodon nigroviridis]" . . . . . 89.74 140 97.14 97.86 1.57e-94 . . . . 17059 2 18 no EMBL CAI73380 . "ubiquitin, putative [Theileria annulata]" . . . . . 50.64 159 97.47 97.47 8.71e-45 . . . . 17059 2 19 no EMBL CAJ83451 . "ribosomal protein S27a [Xenopus (Silurana) tropicalis]" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 20 no GB AAA28997 . "ubiquitin [Drosophila melanogaster]" . . . . . 50.00 231 97.44 98.72 4.75e-44 . . . . 17059 2 21 no GB AAA36788 . "pro-ubiquitin, partial [Homo sapiens]" . . . . . 97.44 152 100.00 100.00 6.60e-106 . . . . 17059 2 22 no GB AAA53067 . "p125 protein, partial [Bovine viral diarrhea virus 1]" . . . . . 50.00 1054 97.44 98.72 2.65e-40 . . . . 17059 2 23 no GB AAA57047 . "ubiquitin, partial [synthetic construct]" . . . . . 100.00 157 100.00 100.00 1.71e-109 . . . . 17059 2 24 no GB AAB03872 . "polyubiquitin, partial [Manduca sexta]" . . . . . 50.00 79 97.44 98.72 8.03e-46 . . . . 17059 2 25 no PIR UQHUR7 . "ubiquitin / ribosomal protein S27a, cytosolic [validated] - human" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 26 no REF NP_001013290 . "ubiquitin [Danio rerio]" . . . . . 50.00 610 97.44 98.72 1.51e-41 . . . . 17059 2 27 no REF NP_001016172 . "ribosomal protein S27a [Xenopus (Silurana) tropicalis]" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 28 no REF NP_001029037 . "ubiquitin-40S ribosomal protein S27a precursor [Mus musculus]" . . . . . 100.00 156 99.36 99.36 9.11e-109 . . . . 17059 2 29 no REF NP_001086065 . "ribosomal protein S27a [Xenopus laevis]" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 30 no REF NP_001129064 . "ubiquitin-40S ribosomal protein S27a precursor [Homo sapiens]" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 31 no SP P0CG50 . "RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Contains: RecName: Full=Ubiquitin-related 1; Contains: RecNam" . . . . . 50.00 734 97.44 98.72 3.76e-41 . . . . 17059 2 32 no SP P0CG69 . "RecName: Full=Polyubiquitin; Contains: RecName: Full=Ubiquitin; Flags: Precursor [Drosophila melanogaster]" . . . . . 50.00 763 97.44 98.72 4.63e-41 . . . . 17059 2 33 no SP P62978 . "RecName: Full=Ubiquitin-40S ribosomal protein S27a; AltName: Full=Ubiquitin carboxyl extension protein 80; Contains: RecName: F" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 34 no SP P62979 . "RecName: Full=Ubiquitin-40S ribosomal protein S27a; AltName: Full=Ubiquitin carboxyl extension protein 80; Contains: RecName: F" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 35 no SP P62982 . "RecName: Full=Ubiquitin-40S ribosomal protein S27a; AltName: Full=Ubiquitin carboxyl extension protein 80; Contains: RecName: F" . . . . . 100.00 156 99.36 99.36 9.11e-109 . . . . 17059 2 36 no TPG DAA24675 . "TPA: 40S ribosomal protein S27a [Bos taurus]" . . . . . 100.00 156 100.00 100.00 1.42e-109 . . . . 17059 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 17059 2 2 . GLN . 17059 2 3 . ILE . 17059 2 4 . PHE . 17059 2 5 . VAL . 17059 2 6 . LYS . 17059 2 7 . THR . 17059 2 8 . LEU . 17059 2 9 . THR . 17059 2 10 . GLY . 17059 2 11 . LYS . 17059 2 12 . THR . 17059 2 13 . ILE . 17059 2 14 . THR . 17059 2 15 . LEU . 17059 2 16 . GLU . 17059 2 17 . VAL . 17059 2 18 . GLU . 17059 2 19 . PRO . 17059 2 20 . SER . 17059 2 21 . ASP . 17059 2 22 . THR . 17059 2 23 . ILE . 17059 2 24 . GLU . 17059 2 25 . ASN . 17059 2 26 . VAL . 17059 2 27 . LYS . 17059 2 28 . ALA . 17059 2 29 . LYS . 17059 2 30 . ILE . 17059 2 31 . GLN . 17059 2 32 . ASP . 17059 2 33 . LYS . 17059 2 34 . GLU . 17059 2 35 . GLY . 17059 2 36 . ILE . 17059 2 37 . PRO . 17059 2 38 . PRO . 17059 2 39 . ASP . 17059 2 40 . GLN . 17059 2 41 . GLN . 17059 2 42 . ARG . 17059 2 43 . LEU . 17059 2 44 . ILE . 17059 2 45 . PHE . 17059 2 46 . ALA . 17059 2 47 . GLY . 17059 2 48 . LYS . 17059 2 49 . GLN . 17059 2 50 . LEU . 17059 2 51 . GLU . 17059 2 52 . ASP . 17059 2 53 . GLY . 17059 2 54 . ARG . 17059 2 55 . THR . 17059 2 56 . LEU . 17059 2 57 . SER . 17059 2 58 . ASP . 17059 2 59 . TYR . 17059 2 60 . ASN . 17059 2 61 . ILE . 17059 2 62 . GLN . 17059 2 63 . LYS . 17059 2 64 . GLU . 17059 2 65 . SER . 17059 2 66 . THR . 17059 2 67 . LEU . 17059 2 68 . HIS . 17059 2 69 . LEU . 17059 2 70 . VAL . 17059 2 71 . LEU . 17059 2 72 . ARG . 17059 2 73 . LEU . 17059 2 74 . ARG . 17059 2 75 . GLY . 17059 2 76 . GLY . 17059 2 77 . ALA . 17059 2 78 . LYS . 17059 2 79 . LYS . 17059 2 80 . ARG . 17059 2 81 . LYS . 17059 2 82 . LYS . 17059 2 83 . LYS . 17059 2 84 . SER . 17059 2 85 . TYR . 17059 2 86 . THR . 17059 2 87 . THR . 17059 2 88 . PRO . 17059 2 89 . LYS . 17059 2 90 . LYS . 17059 2 91 . ASN . 17059 2 92 . LYS . 17059 2 93 . HIS . 17059 2 94 . LYS . 17059 2 95 . ARG . 17059 2 96 . LYS . 17059 2 97 . LYS . 17059 2 98 . VAL . 17059 2 99 . LYS . 17059 2 100 . LEU . 17059 2 101 . ALA . 17059 2 102 . VAL . 17059 2 103 . LEU . 17059 2 104 . LYS . 17059 2 105 . TYR . 17059 2 106 . TYR . 17059 2 107 . LYS . 17059 2 108 . VAL . 17059 2 109 . ASP . 17059 2 110 . GLU . 17059 2 111 . ASN . 17059 2 112 . GLY . 17059 2 113 . LYS . 17059 2 114 . ILE . 17059 2 115 . SER . 17059 2 116 . ARG . 17059 2 117 . LEU . 17059 2 118 . ARG . 17059 2 119 . ARG . 17059 2 120 . GLU . 17059 2 121 . CYS . 17059 2 122 . PRO . 17059 2 123 . SER . 17059 2 124 . ASP . 17059 2 125 . GLU . 17059 2 126 . CYS . 17059 2 127 . GLY . 17059 2 128 . ALA . 17059 2 129 . GLY . 17059 2 130 . VAL . 17059 2 131 . PHE . 17059 2 132 . MET . 17059 2 133 . ALA . 17059 2 134 . SER . 17059 2 135 . HIS . 17059 2 136 . PHE . 17059 2 137 . ASP . 17059 2 138 . ARG . 17059 2 139 . HIS . 17059 2 140 . TYR . 17059 2 141 . CYS . 17059 2 142 . GLY . 17059 2 143 . LYS . 17059 2 144 . CYS . 17059 2 145 . CYS . 17059 2 146 . LEU . 17059 2 147 . THR . 17059 2 148 . TYR . 17059 2 149 . CYS . 17059 2 150 . PHE . 17059 2 151 . ASN . 17059 2 152 . LYS . 17059 2 153 . PRO . 17059 2 154 . GLU . 17059 2 155 . ASP . 17059 2 156 . LYS . 17059 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 17059 2 . GLN 2 2 17059 2 . ILE 3 3 17059 2 . PHE 4 4 17059 2 . VAL 5 5 17059 2 . LYS 6 6 17059 2 . THR 7 7 17059 2 . LEU 8 8 17059 2 . THR 9 9 17059 2 . GLY 10 10 17059 2 . LYS 11 11 17059 2 . THR 12 12 17059 2 . ILE 13 13 17059 2 . THR 14 14 17059 2 . LEU 15 15 17059 2 . GLU 16 16 17059 2 . VAL 17 17 17059 2 . GLU 18 18 17059 2 . PRO 19 19 17059 2 . SER 20 20 17059 2 . ASP 21 21 17059 2 . THR 22 22 17059 2 . ILE 23 23 17059 2 . GLU 24 24 17059 2 . ASN 25 25 17059 2 . VAL 26 26 17059 2 . LYS 27 27 17059 2 . ALA 28 28 17059 2 . LYS 29 29 17059 2 . ILE 30 30 17059 2 . GLN 31 31 17059 2 . ASP 32 32 17059 2 . LYS 33 33 17059 2 . GLU 34 34 17059 2 . GLY 35 35 17059 2 . ILE 36 36 17059 2 . PRO 37 37 17059 2 . PRO 38 38 17059 2 . ASP 39 39 17059 2 . GLN 40 40 17059 2 . GLN 41 41 17059 2 . ARG 42 42 17059 2 . LEU 43 43 17059 2 . ILE 44 44 17059 2 . PHE 45 45 17059 2 . ALA 46 46 17059 2 . GLY 47 47 17059 2 . LYS 48 48 17059 2 . GLN 49 49 17059 2 . LEU 50 50 17059 2 . GLU 51 51 17059 2 . ASP 52 52 17059 2 . GLY 53 53 17059 2 . ARG 54 54 17059 2 . THR 55 55 17059 2 . LEU 56 56 17059 2 . SER 57 57 17059 2 . ASP 58 58 17059 2 . TYR 59 59 17059 2 . ASN 60 60 17059 2 . ILE 61 61 17059 2 . GLN 62 62 17059 2 . LYS 63 63 17059 2 . GLU 64 64 17059 2 . SER 65 65 17059 2 . THR 66 66 17059 2 . LEU 67 67 17059 2 . HIS 68 68 17059 2 . LEU 69 69 17059 2 . VAL 70 70 17059 2 . LEU 71 71 17059 2 . ARG 72 72 17059 2 . LEU 73 73 17059 2 . ARG 74 74 17059 2 . GLY 75 75 17059 2 . GLY 76 76 17059 2 . ALA 77 77 17059 2 . LYS 78 78 17059 2 . LYS 79 79 17059 2 . ARG 80 80 17059 2 . LYS 81 81 17059 2 . LYS 82 82 17059 2 . LYS 83 83 17059 2 . SER 84 84 17059 2 . TYR 85 85 17059 2 . THR 86 86 17059 2 . THR 87 87 17059 2 . PRO 88 88 17059 2 . LYS 89 89 17059 2 . LYS 90 90 17059 2 . ASN 91 91 17059 2 . LYS 92 92 17059 2 . HIS 93 93 17059 2 . LYS 94 94 17059 2 . ARG 95 95 17059 2 . LYS 96 96 17059 2 . LYS 97 97 17059 2 . VAL 98 98 17059 2 . LYS 99 99 17059 2 . LEU 100 100 17059 2 . ALA 101 101 17059 2 . VAL 102 102 17059 2 . LEU 103 103 17059 2 . LYS 104 104 17059 2 . TYR 105 105 17059 2 . TYR 106 106 17059 2 . LYS 107 107 17059 2 . VAL 108 108 17059 2 . ASP 109 109 17059 2 . GLU 110 110 17059 2 . ASN 111 111 17059 2 . GLY 112 112 17059 2 . LYS 113 113 17059 2 . ILE 114 114 17059 2 . SER 115 115 17059 2 . ARG 116 116 17059 2 . LEU 117 117 17059 2 . ARG 118 118 17059 2 . ARG 119 119 17059 2 . GLU 120 120 17059 2 . CYS 121 121 17059 2 . PRO 122 122 17059 2 . SER 123 123 17059 2 . ASP 124 124 17059 2 . GLU 125 125 17059 2 . CYS 126 126 17059 2 . GLY 127 127 17059 2 . ALA 128 128 17059 2 . GLY 129 129 17059 2 . VAL 130 130 17059 2 . PHE 131 131 17059 2 . MET 132 132 17059 2 . ALA 133 133 17059 2 . SER 134 134 17059 2 . HIS 135 135 17059 2 . PHE 136 136 17059 2 . ASP 137 137 17059 2 . ARG 138 138 17059 2 . HIS 139 139 17059 2 . TYR 140 140 17059 2 . CYS 141 141 17059 2 . GLY 142 142 17059 2 . LYS 143 143 17059 2 . CYS 144 144 17059 2 . CYS 145 145 17059 2 . LEU 146 146 17059 2 . THR 147 147 17059 2 . TYR 148 148 17059 2 . CYS 149 149 17059 2 . PHE 150 150 17059 2 . ASN 151 151 17059 2 . LYS 152 152 17059 2 . PRO 153 153 17059 2 . GLU 154 154 17059 2 . ASP 155 155 17059 2 . LYS 156 156 17059 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 17059 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $STAM1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . . . . . . . . . 17059 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 17059 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $STAM1 . 'recombinant technology' 'Escherichia coli' 'E. coli' . 562 Escherichia coli BL21 DE3 . . . . . . . . . . . . . . 'pET-glutathionse S-transferase' . . . . . . 17059 1 2 2 $ubiquitin . 'obtained from a vendor' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17059 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 17059 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details 'ubiquitin was titrated into solution' _Sample.Aggregate_sample_number 8 _Sample.Solvent_system '95% H2O/5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 STAM1 [U-15N] . . 1 $STAM1 . . 0.1 . . mM . . . . 17059 1 2 ubiquitin [U-15N] . . 2 $ubiquitin . . . 0.02 0.5 mM . . . . 17059 1 3 'sodium phosphate' 'natural abundance' . . . . . . 20 . . mM . . . . 17059 1 4 DTT 'natural abundance' . . . . . . 1 . . mM . . . . 17059 1 5 'sodium chloride' 'natural abundance' . . . . . . 0.1 . . M . . . . 17059 1 6 PMSF 'natural abundance' . . . . . . 0.5 . . mM . . . . 17059 1 7 'sodium azide' 'natural abundance' . . . . . . 0.05 . . mM . . . . 17059 1 8 H2O 'natural abundance' . . . . . . 95 . . % . . . . 17059 1 9 D2O 'natural abundance' . . . . . . 5 . . % . . . . 17059 1 stop_ save_ save_sample_2 _Sample.Sf_category sample _Sample.Sf_framecode sample_2 _Sample.Entry_ID 17059 _Sample.ID 2 _Sample.Type solution _Sample.Sub_type . _Sample.Details 'STAM1 was titrated into solution' _Sample.Aggregate_sample_number 6 _Sample.Solvent_system '95% H2O/5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 STAM1 'natural abundance' . . 1 $STAM1 . . . 0.01 0.2 mM . . . . 17059 2 2 ubiquitin [U-15N] . . 2 $ubiquitin . . 0.05 . . mM . . . . 17059 2 3 'sodium phosphate' 'natural abundance' . . . . . . 20 . . mM . . . . 17059 2 4 DTT 'natural abundance' . . . . . . 1 . . mM . . . . 17059 2 5 'sodium chloride' 'natural abundance' . . . . . . 0.1 . . M . . . . 17059 2 6 PMSF 'natural abundance' . . . . . . 0.5 . . mM . . . . 17059 2 7 'sodium azide' 'natural abundance' . . . . . . 0.05 . . mM . . . . 17059 2 8 H2O 'natural abundance' . . . . . . 95 . . % . . . . 17059 2 9 D2O 'natural abundance' . . . . . . 5 . . % . . . . 17059 2 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 17059 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 6.8 . pH 17059 1 pressure 1 . atm 17059 1 temperature 298 . K 17059 1 stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Software.Sf_category software _Software.Sf_framecode NMRPipe _Software.Entry_ID 17059 _Software.ID 1 _Software.Name NMRPipe _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 17059 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 17059 1 stop_ save_ save_NMRView _Software.Sf_category software _Software.Sf_framecode NMRView _Software.Entry_ID 17059 _Software.ID 2 _Software.Name NMRView _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Johnson, One Moon Scientific' . . 17059 2 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 17059 2 stop_ save_ save_KaleidaGraph _Software.Sf_category software _Software.Sf_framecode KaleidaGraph _Software.Entry_ID 17059 _Software.ID 3 _Software.Name KaleidaGraph _Software.Version 3.5 _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Synergy Software' . . 17059 3 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 17059 3 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 17059 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DRX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 500 save_ save_spectrometer_2 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_2 _NMR_spectrometer.Entry_ID 17059 _NMR_spectrometer.ID 2 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model Avance _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 17059 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker DRX . 500 . . . 17059 1 2 spectrometer_2 Bruker Avance . 600 . . . 17059 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 17059 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 17059 1 2 '2D 1H-15N HSQC' no . . . . . . . . . . 2 $sample_2 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 17059 1 stop_ save_ save_binding_data _Binding_value_list.Sf_category binding_data _Binding_value_list.Sf_framecode binding_data _Binding_value_list.Entry_ID 17059 _Binding_value_list.ID 1 _Binding_value_list.Sample_condition_list_ID 1 _Binding_value_list.Sample_condition_list_label $sample_conditions_1 _Binding_value_list.Details . _Binding_value_list.Text_data_format . _Binding_value_list.Text_data . loop_ _Binding_experiment.Experiment_ID _Binding_experiment.Experiment_name _Binding_experiment.Sample_ID _Binding_experiment.Sample_label _Binding_experiment.Sample_state _Binding_experiment.Entry_ID _Binding_experiment.Binding_value_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 17059 1 2 '2D 1H-15N HSQC' 2 $sample_2 isotropic 17059 1 stop_ loop_ _Binding_software.Software_ID _Binding_software.Software_label _Binding_software.Method_ID _Binding_software.Method_label _Binding_software.Entry_ID _Binding_software.Binding_value_list_ID 1 $NMRPipe . . 17059 1 2 $NMRView . . 17059 1 3 $KaleidaGraph . . 17059 1 stop_ loop_ _Binding_result.ID _Binding_result.Experiment_ID _Binding_result.Assembly_ID _Binding_result.Atm_obs_assembly_atom_ID _Binding_result.Atm_obs_entity_assembly_ID _Binding_result.Atm_obs_entity_ID _Binding_result.Atm_obs_comp_index_ID _Binding_result.Atm_obs_seq_ID _Binding_result.Atm_obs_comp_ID _Binding_result.Atm_obs_atom_ID _Binding_result.Atm_obs_atom_type _Binding_result.Atm_obs_atom_isotope_number _Binding_result.Resonance_ID _Binding_result.Atm_obs_auth_entity_assembly_ID _Binding_result.Atm_obs_auth_seq_ID _Binding_result.Atm_obs_auth_comp_ID _Binding_result.Atm_obs_auth_atom_ID _Binding_result.Expt_observed_param _Binding_result.Val_type _Binding_result.Val _Binding_result.Val_err _Binding_result.Val_units _Binding_result.Entry_ID _Binding_result.Binding_value_list_ID 1 1 1 . 1 1 . 77 GLY . . . . . . . . 'chemical shifts' Kd 52 10 uM 17059 1 2 2 1 . 1 2 . . . . . . . . . . . 'chemical shifts' Kd 41 6 uM 17059 1 3 1 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Ka 50 . uM 17059 1 4 2 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Ka 50 . uM 17059 1 stop_ loop_ _Binding_partners.Binding_result_ID _Binding_partners.Assembly_ID _Binding_partners.Entity_assembly_ID _Binding_partners.Entity_assembly_name _Binding_partners.Entity_ID _Binding_partners.Entity_label _Binding_partners.Entry_ID _Binding_partners.Binding_value_list_ID 1 1 1 STAM1 1 $STAM1 17059 1 1 1 2 ubiquitin 2 $ubiquitin 17059 1 2 1 1 STAM1 1 $STAM1 17059 1 2 1 2 ubiquitin 2 $ubiquitin 17059 1 3 1 1 STAM1 1 $STAM1 17059 1 3 1 2 ubiquitin 2 $ubiquitin 17059 1 4 1 1 STAM1 1 $STAM1 17059 1 4 1 2 ubiquitin 2 $ubiquitin 17059 1 stop_ save_