data_17060 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 17060 _Entry.Title ; Replication protein A 32 interacts through a similar binding interface with TIPIN, XPA, and UNG2 ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2010-07-13 _Entry.Accession_date 2010-07-13 _Entry.Last_release_date 2010-09-08 _Entry.Original_release_date 2010-09-08 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Seikh Ali . I. . 17060 2 Jae-Sun Shin . . . 17060 3 Sung-Hun Bae . . . 17060 4 Byoungkook Kim . . . 17060 5 Byong-Seok Choi . . . 17060 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID binding_constants 1 17060 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'binding constants' 1 17060 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2010-09-08 2010-07-13 original author . 17060 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 17060 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID . _Citation.Full_citation . _Citation.Title 'Replication protein A 32 interacts through a similar binding interface with TIPIN, XPA, and UNG2' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'INT. J. BIOCHEM. CELL B' _Citation.Journal_name_full . _Citation.Journal_volume 42 _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 1210 _Citation.Page_last 1215 _Citation.Year 2010 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Seikh Ali . I. . 17060 1 2 Jae-Sun Shin . . . 17060 1 3 Sung-Hun Bae . . . 17060 1 4 Byoungkook Kim . . . 17060 1 5 Byong-Seok Choi . . . 17060 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 17060 _Assembly.ID 1 _Assembly.Name RPA32C/TIPIN _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 RPA32C 1 $RPA32C A . yes native no no . . . 17060 1 2 TIPIN 2 $TIPIN A . yes native no no . . . 17060 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_RPA32C _Entity.Sf_category entity _Entity.Sf_framecode RPA32C _Entity.Entry_ID 17060 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name RPA32C _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; SQPSAGRAPISNPGMSEAGN FGGNSFMPANGLTVAQNQVL NLIKACPRPEGLNFQDLKNQ LKHMSVSSIKQAVDFLSNEG HIYSTVDDDHFKSTDAE ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 97 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 4460 . "Replication Protein A" . . . . . 100.00 103 100.00 100.00 4.06e-64 . . . . 17060 1 2 no PDB 1DPU . "Solution Structure Of The C-Terminal Domain Of Human Rpa32 Complexed With Ung2(73-88)" . . . . . 100.00 99 100.00 100.00 3.80e-64 . . . . 17060 1 3 no PDB 1Z1D . "Structural Model For The Interaction Between Rpa32 C- Terminal Domain And Sv40 T Antigen Origin Binding Domain" . . . . . 100.00 103 100.00 100.00 4.06e-64 . . . . 17060 1 4 no PDB 2PI2 . "Full-Length Replication Protein A Subunits Rpa14 And Rpa32" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 5 no PDB 2Z6K . "Crystal Structure Of Full-Length Human Rpa1432 HETERODIMER" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 6 no PDB 4MQV . "Crystal Complex Of Rpa32c And Smarcal1 N-terminus" . . . . . 71.13 69 100.00 100.00 3.87e-42 . . . . 17060 1 7 no PDB 4OU0 . "Crystal Structure Of Rpa32c" . . . . . 71.13 73 98.55 98.55 2.48e-40 . . . . 17060 1 8 no DBJ BAG35795 . "unnamed protein product [Homo sapiens]" . . . . . 100.00 270 98.97 100.00 3.43e-62 . . . . 17060 1 9 no DBJ BAG59456 . "unnamed protein product [Homo sapiens]" . . . . . 100.00 104 100.00 100.00 2.68e-64 . . . . 17060 1 10 no DBJ BAG60901 . "unnamed protein product [Homo sapiens]" . . . . . 100.00 264 100.00 100.00 7.87e-63 . . . . 17060 1 11 no DBJ BAG62374 . "unnamed protein product [Homo sapiens]" . . . . . 100.00 174 100.00 100.00 5.11e-64 . . . . 17060 1 12 no DBJ BAI47325 . "replication protein A2, 32kDa [synthetic construct]" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 13 no EMBL CAG29344 . "RPA2 [Homo sapiens]" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 14 no EMBL CAH90667 . "hypothetical protein [Pongo abelii]" . . . . . 100.00 270 97.94 100.00 6.15e-62 . . . . 17060 1 15 no GB AAA36560 . "replication protein A [Homo sapiens]" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 16 no GB AAH01630 . "Replication protein A2, 32kDa [Homo sapiens]" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 17 no GB AAH12157 . "Replication protein A2, 32kDa [Homo sapiens]" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 18 no GB AAH21257 . "Replication protein A2, 32kDa [Homo sapiens]" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 19 no GB AAX84514 . "replication protein A2, 32kDa [Homo sapiens]" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 20 no REF NP_001125362 . "replication protein A 32 kDa subunit [Pongo abelii]" . . . . . 100.00 343 97.94 100.00 2.09e-61 . . . . 17060 1 21 no REF NP_001247563 . "replication protein A 32 kDa subunit [Macaca mulatta]" . . . . . 100.00 270 98.97 100.00 3.78e-62 . . . . 17060 1 22 no REF NP_001273005 . "replication protein A 32 kDa subunit isoform 2 [Homo sapiens]" . . . . . 100.00 174 100.00 100.00 5.11e-64 . . . . 17060 1 23 no REF NP_001284487 . "replication protein A 32 kDa subunit isoform 3 [Homo sapiens]" . . . . . 100.00 278 100.00 100.00 1.20e-62 . . . . 17060 1 24 no REF NP_002937 . "replication protein A 32 kDa subunit isoform 1 [Homo sapiens]" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 25 no SP P15927 . "RecName: Full=Replication protein A 32 kDa subunit; Short=RP-A p32; AltName: Full=Replication factor A protein 2; Short=RF-A pr" . . . . . 100.00 270 100.00 100.00 9.99e-63 . . . . 17060 1 26 no SP Q5RC43 . "RecName: Full=Replication protein A 32 kDa subunit; Short=RP-A p32; AltName: Full=Replication factor A protein 2; Short=RF-A pr" . . . . . 100.00 270 97.94 100.00 6.15e-62 . . . . 17060 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . SER . 17060 1 2 . GLN . 17060 1 3 . PRO . 17060 1 4 . SER . 17060 1 5 . ALA . 17060 1 6 . GLY . 17060 1 7 . ARG . 17060 1 8 . ALA . 17060 1 9 . PRO . 17060 1 10 . ILE . 17060 1 11 . SER . 17060 1 12 . ASN . 17060 1 13 . PRO . 17060 1 14 . GLY . 17060 1 15 . MET . 17060 1 16 . SER . 17060 1 17 . GLU . 17060 1 18 . ALA . 17060 1 19 . GLY . 17060 1 20 . ASN . 17060 1 21 . PHE . 17060 1 22 . GLY . 17060 1 23 . GLY . 17060 1 24 . ASN . 17060 1 25 . SER . 17060 1 26 . PHE . 17060 1 27 . MET . 17060 1 28 . PRO . 17060 1 29 . ALA . 17060 1 30 . ASN . 17060 1 31 . GLY . 17060 1 32 . LEU . 17060 1 33 . THR . 17060 1 34 . VAL . 17060 1 35 . ALA . 17060 1 36 . GLN . 17060 1 37 . ASN . 17060 1 38 . GLN . 17060 1 39 . VAL . 17060 1 40 . LEU . 17060 1 41 . ASN . 17060 1 42 . LEU . 17060 1 43 . ILE . 17060 1 44 . LYS . 17060 1 45 . ALA . 17060 1 46 . CYS . 17060 1 47 . PRO . 17060 1 48 . ARG . 17060 1 49 . PRO . 17060 1 50 . GLU . 17060 1 51 . GLY . 17060 1 52 . LEU . 17060 1 53 . ASN . 17060 1 54 . PHE . 17060 1 55 . GLN . 17060 1 56 . ASP . 17060 1 57 . LEU . 17060 1 58 . LYS . 17060 1 59 . ASN . 17060 1 60 . GLN . 17060 1 61 . LEU . 17060 1 62 . LYS . 17060 1 63 . HIS . 17060 1 64 . MET . 17060 1 65 . SER . 17060 1 66 . VAL . 17060 1 67 . SER . 17060 1 68 . SER . 17060 1 69 . ILE . 17060 1 70 . LYS . 17060 1 71 . GLN . 17060 1 72 . ALA . 17060 1 73 . VAL . 17060 1 74 . ASP . 17060 1 75 . PHE . 17060 1 76 . LEU . 17060 1 77 . SER . 17060 1 78 . ASN . 17060 1 79 . GLU . 17060 1 80 . GLY . 17060 1 81 . HIS . 17060 1 82 . ILE . 17060 1 83 . TYR . 17060 1 84 . SER . 17060 1 85 . THR . 17060 1 86 . VAL . 17060 1 87 . ASP . 17060 1 88 . ASP . 17060 1 89 . ASP . 17060 1 90 . HIS . 17060 1 91 . PHE . 17060 1 92 . LYS . 17060 1 93 . SER . 17060 1 94 . THR . 17060 1 95 . ASP . 17060 1 96 . ALA . 17060 1 97 . GLU . 17060 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . SER 1 1 17060 1 . GLN 2 2 17060 1 . PRO 3 3 17060 1 . SER 4 4 17060 1 . ALA 5 5 17060 1 . GLY 6 6 17060 1 . ARG 7 7 17060 1 . ALA 8 8 17060 1 . PRO 9 9 17060 1 . ILE 10 10 17060 1 . SER 11 11 17060 1 . ASN 12 12 17060 1 . PRO 13 13 17060 1 . GLY 14 14 17060 1 . MET 15 15 17060 1 . SER 16 16 17060 1 . GLU 17 17 17060 1 . ALA 18 18 17060 1 . GLY 19 19 17060 1 . ASN 20 20 17060 1 . PHE 21 21 17060 1 . GLY 22 22 17060 1 . GLY 23 23 17060 1 . ASN 24 24 17060 1 . SER 25 25 17060 1 . PHE 26 26 17060 1 . MET 27 27 17060 1 . PRO 28 28 17060 1 . ALA 29 29 17060 1 . ASN 30 30 17060 1 . GLY 31 31 17060 1 . LEU 32 32 17060 1 . THR 33 33 17060 1 . VAL 34 34 17060 1 . ALA 35 35 17060 1 . GLN 36 36 17060 1 . ASN 37 37 17060 1 . GLN 38 38 17060 1 . VAL 39 39 17060 1 . LEU 40 40 17060 1 . ASN 41 41 17060 1 . LEU 42 42 17060 1 . ILE 43 43 17060 1 . LYS 44 44 17060 1 . ALA 45 45 17060 1 . CYS 46 46 17060 1 . PRO 47 47 17060 1 . ARG 48 48 17060 1 . PRO 49 49 17060 1 . GLU 50 50 17060 1 . GLY 51 51 17060 1 . LEU 52 52 17060 1 . ASN 53 53 17060 1 . PHE 54 54 17060 1 . GLN 55 55 17060 1 . ASP 56 56 17060 1 . LEU 57 57 17060 1 . LYS 58 58 17060 1 . ASN 59 59 17060 1 . GLN 60 60 17060 1 . LEU 61 61 17060 1 . LYS 62 62 17060 1 . HIS 63 63 17060 1 . MET 64 64 17060 1 . SER 65 65 17060 1 . VAL 66 66 17060 1 . SER 67 67 17060 1 . SER 68 68 17060 1 . ILE 69 69 17060 1 . LYS 70 70 17060 1 . GLN 71 71 17060 1 . ALA 72 72 17060 1 . VAL 73 73 17060 1 . ASP 74 74 17060 1 . PHE 75 75 17060 1 . LEU 76 76 17060 1 . SER 77 77 17060 1 . ASN 78 78 17060 1 . GLU 79 79 17060 1 . GLY 80 80 17060 1 . HIS 81 81 17060 1 . ILE 82 82 17060 1 . TYR 83 83 17060 1 . SER 84 84 17060 1 . THR 85 85 17060 1 . VAL 86 86 17060 1 . ASP 87 87 17060 1 . ASP 88 88 17060 1 . ASP 89 89 17060 1 . HIS 90 90 17060 1 . PHE 91 91 17060 1 . LYS 92 92 17060 1 . SER 93 93 17060 1 . THR 94 94 17060 1 . ASP 95 95 17060 1 . ALA 96 96 17060 1 . GLU 97 97 17060 1 stop_ save_ save_TIPIN _Entity.Sf_category entity _Entity.Sf_framecode TIPIN _Entity.Entry_ID 17060 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name TIPIN _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; EMFASELSRSLTEEQQRRIE RNKQLALERRQAKL ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 34 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-01-30 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no DBJ BAA91229 . "unnamed protein product [Homo sapiens]" . . . . . 100.00 301 100.00 100.00 1.37e-11 . . . . 17060 2 2 no DBJ BAG61265 . "unnamed protein product [Homo sapiens]" . . . . . 100.00 200 97.06 100.00 4.05e-11 . . . . 17060 2 3 no DBJ BAI46472 . "TIMELESS interacting protein [synthetic construct]" . . . . . 100.00 301 97.06 100.00 7.22e-11 . . . . 17060 2 4 no EMBL CAL38286 . "hypothetical protein [synthetic construct]" . . . . . 100.00 301 97.06 100.00 7.22e-11 . . . . 17060 2 5 no GB AAH00870 . "TIMELESS interacting protein [Homo sapiens]" . . . . . 100.00 301 97.06 100.00 7.22e-11 . . . . 17060 2 6 no GB ACB21044 . "TIMELESS interacting protein [Homo sapiens]" . . . . . 100.00 301 97.06 100.00 7.69e-11 . . . . 17060 2 7 no GB AIC51688 . "TIPIN, partial [synthetic construct]" . . . . . 100.00 301 97.06 100.00 7.22e-11 . . . . 17060 2 8 no GB EAW77762 . "timeless-interacting protein, isoform CRA_a [Homo sapiens]" . . . . . 100.00 301 97.06 100.00 7.22e-11 . . . . 17060 2 9 no REF NP_001276915 . "TIMELESS-interacting protein isoform 2 [Homo sapiens]" . . . . . 100.00 200 97.06 100.00 4.05e-11 . . . . 17060 2 10 no REF NP_060328 . "TIMELESS-interacting protein isoform 1 [Homo sapiens]" . . . . . 100.00 301 97.06 100.00 7.22e-11 . . . . 17060 2 11 no REF XP_005254578 . "PREDICTED: TIMELESS-interacting protein isoform X1 [Homo sapiens]" . . . . . 100.00 301 97.06 100.00 7.69e-11 . . . . 17060 2 12 no REF XP_005254579 . "PREDICTED: TIMELESS-interacting protein isoform X2 [Homo sapiens]" . . . . . 100.00 301 97.06 100.00 7.69e-11 . . . . 17060 2 13 no REF XP_006720656 . "PREDICTED: TIMELESS-interacting protein isoform X4 [Homo sapiens]" . . . . . 100.00 301 97.06 100.00 7.69e-11 . . . . 17060 2 14 no SP Q9BVW5 . "RecName: Full=TIMELESS-interacting protein [Homo sapiens]" . . . . . 100.00 301 97.06 100.00 7.69e-11 . . . . 17060 2 15 no TPG DAA01365 . "TPA_exp: timeless-interacting protein [Homo sapiens]" . . . . . 100.00 301 97.06 100.00 7.22e-11 . . . . 17060 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . GLU . 17060 2 2 . MET . 17060 2 3 . PHE . 17060 2 4 . ALA . 17060 2 5 . SER . 17060 2 6 . GLU . 17060 2 7 . LEU . 17060 2 8 . SER . 17060 2 9 . ARG . 17060 2 10 . SER . 17060 2 11 . LEU . 17060 2 12 . THR . 17060 2 13 . GLU . 17060 2 14 . GLU . 17060 2 15 . GLN . 17060 2 16 . GLN . 17060 2 17 . ARG . 17060 2 18 . ARG . 17060 2 19 . ILE . 17060 2 20 . GLU . 17060 2 21 . ARG . 17060 2 22 . ASN . 17060 2 23 . LYS . 17060 2 24 . GLN . 17060 2 25 . LEU . 17060 2 26 . ALA . 17060 2 27 . LEU . 17060 2 28 . GLU . 17060 2 29 . ARG . 17060 2 30 . ARG . 17060 2 31 . GLN . 17060 2 32 . ALA . 17060 2 33 . LYS . 17060 2 34 . LEU . 17060 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLU 1 1 17060 2 . MET 2 2 17060 2 . PHE 3 3 17060 2 . ALA 4 4 17060 2 . SER 5 5 17060 2 . GLU 6 6 17060 2 . LEU 7 7 17060 2 . SER 8 8 17060 2 . ARG 9 9 17060 2 . SER 10 10 17060 2 . LEU 11 11 17060 2 . THR 12 12 17060 2 . GLU 13 13 17060 2 . GLU 14 14 17060 2 . GLN 15 15 17060 2 . GLN 16 16 17060 2 . ARG 17 17 17060 2 . ARG 18 18 17060 2 . ILE 19 19 17060 2 . GLU 20 20 17060 2 . ARG 21 21 17060 2 . ASN 22 22 17060 2 . LYS 23 23 17060 2 . GLN 24 24 17060 2 . LEU 25 25 17060 2 . ALA 26 26 17060 2 . LEU 27 27 17060 2 . GLU 28 28 17060 2 . ARG 29 29 17060 2 . ARG 30 30 17060 2 . GLN 31 31 17060 2 . ALA 32 32 17060 2 . LYS 33 33 17060 2 . LEU 34 34 17060 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 17060 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $RPA32C . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . . . . . . . . . 17060 1 2 2 $TIPIN . . 'no natural source' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17060 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 17060 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $RPA32C . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . . . . . . . . . . . . . . pET15b . . . . . . 17060 1 2 2 $TIPIN . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . . . . . . . . . . . . . . pGEX4T1 . . . . . . 17060 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 17060 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details 'TIPIN was titrated into solution' _Sample.Aggregate_sample_number . _Sample.Solvent_system H2O/D2O _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'sodium phosphate' 'natural abundance' . . . . . . 25 . . mM . . . . 17060 1 2 'sodium chloride' 'natural abundance' . . . . . . 50 . . mM . . . . 17060 1 3 DTT 'natural abundance' . . . . . . 3 . . mM . . . . 17060 1 4 RPA32C [U-15N] . . 1 $RPA32C . . 0.1 . . mM . . . . 17060 1 5 TIPIN 'natural abundance' . . 2 $TIPIN . . . 0.02 3.0 mM . . . . 17060 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 17060 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 7.0 . pH 17060 1 pressure 1 . atm 17060 1 temperature 293 . K 17060 1 stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Software.Sf_category software _Software.Sf_framecode SPARKY _Software.Entry_ID 17060 _Software.ID 1 _Software.Name SPARKY _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID Goddard . . 17060 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 17060 1 stop_ save_ save_NMRPipe _Software.Sf_category software _Software.Sf_framecode NMRPipe _Software.Entry_ID 17060 _Software.ID 2 _Software.Name NMRPipe _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 17060 2 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 17060 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 17060 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model 'not given' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_spectrometer_2 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_2 _NMR_spectrometer.Entry_ID 17060 _NMR_spectrometer.ID 2 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'not given' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 800 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 17060 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Varian 'not given' . 600 . . . 17060 1 2 spectrometer_2 Bruker 'not given' . 800 . . . 17060 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 17060 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 17060 1 stop_ save_ save_binding_data _Binding_value_list.Sf_category binding_data _Binding_value_list.Sf_framecode binding_data _Binding_value_list.Entry_ID 17060 _Binding_value_list.ID 1 _Binding_value_list.Sample_condition_list_ID 1 _Binding_value_list.Sample_condition_list_label $sample_conditions_1 _Binding_value_list.Details . _Binding_value_list.Text_data_format . _Binding_value_list.Text_data . loop_ _Binding_experiment.Experiment_ID _Binding_experiment.Experiment_name _Binding_experiment.Sample_ID _Binding_experiment.Sample_label _Binding_experiment.Sample_state _Binding_experiment.Entry_ID _Binding_experiment.Binding_value_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 17060 1 stop_ loop_ _Binding_software.Software_ID _Binding_software.Software_label _Binding_software.Method_ID _Binding_software.Method_label _Binding_software.Entry_ID _Binding_software.Binding_value_list_ID 1 $SPARKY . . 17060 1 2 $NMRPipe . . 17060 1 stop_ loop_ _Binding_result.ID _Binding_result.Experiment_ID _Binding_result.Assembly_ID _Binding_result.Atm_obs_assembly_atom_ID _Binding_result.Atm_obs_entity_assembly_ID _Binding_result.Atm_obs_entity_ID _Binding_result.Atm_obs_comp_index_ID _Binding_result.Atm_obs_seq_ID _Binding_result.Atm_obs_comp_ID _Binding_result.Atm_obs_atom_ID _Binding_result.Atm_obs_atom_type _Binding_result.Atm_obs_atom_isotope_number _Binding_result.Resonance_ID _Binding_result.Atm_obs_auth_entity_assembly_ID _Binding_result.Atm_obs_auth_seq_ID _Binding_result.Atm_obs_auth_comp_ID _Binding_result.Atm_obs_auth_atom_ID _Binding_result.Expt_observed_param _Binding_result.Val_type _Binding_result.Val _Binding_result.Val_err _Binding_result.Val_units _Binding_result.Entry_ID _Binding_result.Binding_value_list_ID 1 1 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kd 0.5 0.19 uM 17060 1 stop_ loop_ _Binding_partners.Binding_result_ID _Binding_partners.Assembly_ID _Binding_partners.Entity_assembly_ID _Binding_partners.Entity_assembly_name _Binding_partners.Entity_ID _Binding_partners.Entity_label _Binding_partners.Entry_ID _Binding_partners.Binding_value_list_ID 1 1 1 RPA32C 1 $RPA32C 17060 1 1 1 2 TIPIN 2 $TIPIN 17060 1 stop_ save_