data_17114 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 17114 _Entry.Title ; Binding Kinetics of Histone Chaperone Chz1 and Variant Histone H2A.Z-H2B by Relaxation Dispersion NMR Spectroscopy ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2010-08-11 _Entry.Accession_date 2010-08-11 _Entry.Last_release_date 2010-11-10 _Entry.Original_release_date 2010-11-10 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 D. Hansen . F. . 17114 2 Zheng Zhou . . . 17114 3 Haniqiao Feng . . . 17114 4 Lisa Jenkins . M.M. . 17114 5 Yawen Bai . . . 17114 6 Lewis Kay . E. . 17114 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID 'chemical rates' 1 17114 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'binding constants' 2 17114 'kinetic rates' 3 17114 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2010-11-10 2010-08-11 original author . 17114 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 17114 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 19385041 _Citation.Full_citation . _Citation.Title 'Binding Kinetics of Histone Chaperone Chz1 and Variant Histone H2A.Z-H2B by Relaxation Dispersion NMR Spectroscopy' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'J. Mol. Biol.' _Citation.Journal_name_full 'Journal of Molecular Biology' _Citation.Journal_volume 387 _Citation.Journal_issue 1 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 1 _Citation.Page_last 9 _Citation.Year 2009 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 D. Hansen . F. . 17114 1 2 Zheng Zhou . . . 17114 1 3 Haniqiao Feng . . . 17114 1 4 Lisa Jenkins . M.M. . 17114 1 5 Yawen Bai . . . 17114 1 6 Lewis Kay . E. . 17114 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 17114 _Assembly.ID 1 _Assembly.Name 'H2AZH2B/Chz1 complex' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 H2AZH2B 1 $H2AZH2B A . yes native no no . . . 17114 1 2 Chz1 2 $Chz1 B . yes native no no . . . 17114 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_H2AZH2B _Entity.Sf_category entity _Entity.Sf_framecode H2AZH2B _Entity.Entry_ID 17114 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name H2AZH2B _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; RKETYSSYIYKVLKQTHPDT GISQKSMSILNSFVNDIFER IATEASKLAAYNKKSTISAR EIQTAVRLILPGELAKHAVS EGTRAVTKYSSSTQAQSSSA RAGLQFPVGRIKRYLKRHAT GRTRVGSKAAIYLTAVLEYL TAEVLELAGNAAKDLKVKRI TPRHLQLAIRGDDELDSLIR ATIASGGVLPHI ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 192 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 15393 . H2A.Z-H2B . . . . . 100.00 200 100.00 100.00 1.53e-133 . . . . 17114 1 2 no PDB 2JSS . "Nmr Structure Of Chaperone Chz1 Complexed With Histone H2a.Z-H2b" . . . . . 100.00 192 100.00 100.00 1.97e-133 . . . . 17114 1 3 no PDB 4M6B . "Crystal Structure Of Yeast Swr1-z Domain In Complex With H2a.z-h2b Dimer" . . . . . 100.00 193 100.00 100.00 1.59e-133 . . . . 17114 1 4 no EMBL CAY78724 . "Htb1p [Saccharomyces cerevisiae EC1118]" . . . . . 50.00 132 100.00 100.00 8.25e-60 . . . . 17114 1 5 no GB AJV19600 . "Htb1p [Saccharomyces cerevisiae YJM1574]" . . . . . 50.00 132 100.00 100.00 8.25e-60 . . . . 17114 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . ARG . 17114 1 2 . LYS . 17114 1 3 . GLU . 17114 1 4 . THR . 17114 1 5 . TYR . 17114 1 6 . SER . 17114 1 7 . SER . 17114 1 8 . TYR . 17114 1 9 . ILE . 17114 1 10 . TYR . 17114 1 11 . LYS . 17114 1 12 . VAL . 17114 1 13 . LEU . 17114 1 14 . LYS . 17114 1 15 . GLN . 17114 1 16 . THR . 17114 1 17 . HIS . 17114 1 18 . PRO . 17114 1 19 . ASP . 17114 1 20 . THR . 17114 1 21 . GLY . 17114 1 22 . ILE . 17114 1 23 . SER . 17114 1 24 . GLN . 17114 1 25 . LYS . 17114 1 26 . SER . 17114 1 27 . MET . 17114 1 28 . SER . 17114 1 29 . ILE . 17114 1 30 . LEU . 17114 1 31 . ASN . 17114 1 32 . SER . 17114 1 33 . PHE . 17114 1 34 . VAL . 17114 1 35 . ASN . 17114 1 36 . ASP . 17114 1 37 . ILE . 17114 1 38 . PHE . 17114 1 39 . GLU . 17114 1 40 . ARG . 17114 1 41 . ILE . 17114 1 42 . ALA . 17114 1 43 . THR . 17114 1 44 . GLU . 17114 1 45 . ALA . 17114 1 46 . SER . 17114 1 47 . LYS . 17114 1 48 . LEU . 17114 1 49 . ALA . 17114 1 50 . ALA . 17114 1 51 . TYR . 17114 1 52 . ASN . 17114 1 53 . LYS . 17114 1 54 . LYS . 17114 1 55 . SER . 17114 1 56 . THR . 17114 1 57 . ILE . 17114 1 58 . SER . 17114 1 59 . ALA . 17114 1 60 . ARG . 17114 1 61 . GLU . 17114 1 62 . ILE . 17114 1 63 . GLN . 17114 1 64 . THR . 17114 1 65 . ALA . 17114 1 66 . VAL . 17114 1 67 . ARG . 17114 1 68 . LEU . 17114 1 69 . ILE . 17114 1 70 . LEU . 17114 1 71 . PRO . 17114 1 72 . GLY . 17114 1 73 . GLU . 17114 1 74 . LEU . 17114 1 75 . ALA . 17114 1 76 . LYS . 17114 1 77 . HIS . 17114 1 78 . ALA . 17114 1 79 . VAL . 17114 1 80 . SER . 17114 1 81 . GLU . 17114 1 82 . GLY . 17114 1 83 . THR . 17114 1 84 . ARG . 17114 1 85 . ALA . 17114 1 86 . VAL . 17114 1 87 . THR . 17114 1 88 . LYS . 17114 1 89 . TYR . 17114 1 90 . SER . 17114 1 91 . SER . 17114 1 92 . SER . 17114 1 93 . THR . 17114 1 94 . GLN . 17114 1 95 . ALA . 17114 1 96 . GLN . 17114 1 97 . SER . 17114 1 98 . SER . 17114 1 99 . SER . 17114 1 100 . ALA . 17114 1 101 . ARG . 17114 1 102 . ALA . 17114 1 103 . GLY . 17114 1 104 . LEU . 17114 1 105 . GLN . 17114 1 106 . PHE . 17114 1 107 . PRO . 17114 1 108 . VAL . 17114 1 109 . GLY . 17114 1 110 . ARG . 17114 1 111 . ILE . 17114 1 112 . LYS . 17114 1 113 . ARG . 17114 1 114 . TYR . 17114 1 115 . LEU . 17114 1 116 . LYS . 17114 1 117 . ARG . 17114 1 118 . HIS . 17114 1 119 . ALA . 17114 1 120 . THR . 17114 1 121 . GLY . 17114 1 122 . ARG . 17114 1 123 . THR . 17114 1 124 . ARG . 17114 1 125 . VAL . 17114 1 126 . GLY . 17114 1 127 . SER . 17114 1 128 . LYS . 17114 1 129 . ALA . 17114 1 130 . ALA . 17114 1 131 . ILE . 17114 1 132 . TYR . 17114 1 133 . LEU . 17114 1 134 . THR . 17114 1 135 . ALA . 17114 1 136 . VAL . 17114 1 137 . LEU . 17114 1 138 . GLU . 17114 1 139 . TYR . 17114 1 140 . LEU . 17114 1 141 . THR . 17114 1 142 . ALA . 17114 1 143 . GLU . 17114 1 144 . VAL . 17114 1 145 . LEU . 17114 1 146 . GLU . 17114 1 147 . LEU . 17114 1 148 . ALA . 17114 1 149 . GLY . 17114 1 150 . ASN . 17114 1 151 . ALA . 17114 1 152 . ALA . 17114 1 153 . LYS . 17114 1 154 . ASP . 17114 1 155 . LEU . 17114 1 156 . LYS . 17114 1 157 . VAL . 17114 1 158 . LYS . 17114 1 159 . ARG . 17114 1 160 . ILE . 17114 1 161 . THR . 17114 1 162 . PRO . 17114 1 163 . ARG . 17114 1 164 . HIS . 17114 1 165 . LEU . 17114 1 166 . GLN . 17114 1 167 . LEU . 17114 1 168 . ALA . 17114 1 169 . ILE . 17114 1 170 . ARG . 17114 1 171 . GLY . 17114 1 172 . ASP . 17114 1 173 . ASP . 17114 1 174 . GLU . 17114 1 175 . LEU . 17114 1 176 . ASP . 17114 1 177 . SER . 17114 1 178 . LEU . 17114 1 179 . ILE . 17114 1 180 . ARG . 17114 1 181 . ALA . 17114 1 182 . THR . 17114 1 183 . ILE . 17114 1 184 . ALA . 17114 1 185 . SER . 17114 1 186 . GLY . 17114 1 187 . GLY . 17114 1 188 . VAL . 17114 1 189 . LEU . 17114 1 190 . PRO . 17114 1 191 . HIS . 17114 1 192 . ILE . 17114 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . ARG 1 1 17114 1 . LYS 2 2 17114 1 . GLU 3 3 17114 1 . THR 4 4 17114 1 . TYR 5 5 17114 1 . SER 6 6 17114 1 . SER 7 7 17114 1 . TYR 8 8 17114 1 . ILE 9 9 17114 1 . TYR 10 10 17114 1 . LYS 11 11 17114 1 . VAL 12 12 17114 1 . LEU 13 13 17114 1 . LYS 14 14 17114 1 . GLN 15 15 17114 1 . THR 16 16 17114 1 . HIS 17 17 17114 1 . PRO 18 18 17114 1 . ASP 19 19 17114 1 . THR 20 20 17114 1 . GLY 21 21 17114 1 . ILE 22 22 17114 1 . SER 23 23 17114 1 . GLN 24 24 17114 1 . LYS 25 25 17114 1 . SER 26 26 17114 1 . MET 27 27 17114 1 . SER 28 28 17114 1 . ILE 29 29 17114 1 . LEU 30 30 17114 1 . ASN 31 31 17114 1 . SER 32 32 17114 1 . PHE 33 33 17114 1 . VAL 34 34 17114 1 . ASN 35 35 17114 1 . ASP 36 36 17114 1 . ILE 37 37 17114 1 . PHE 38 38 17114 1 . GLU 39 39 17114 1 . ARG 40 40 17114 1 . ILE 41 41 17114 1 . ALA 42 42 17114 1 . THR 43 43 17114 1 . GLU 44 44 17114 1 . ALA 45 45 17114 1 . SER 46 46 17114 1 . LYS 47 47 17114 1 . LEU 48 48 17114 1 . ALA 49 49 17114 1 . ALA 50 50 17114 1 . TYR 51 51 17114 1 . ASN 52 52 17114 1 . LYS 53 53 17114 1 . LYS 54 54 17114 1 . SER 55 55 17114 1 . THR 56 56 17114 1 . ILE 57 57 17114 1 . SER 58 58 17114 1 . ALA 59 59 17114 1 . ARG 60 60 17114 1 . GLU 61 61 17114 1 . ILE 62 62 17114 1 . GLN 63 63 17114 1 . THR 64 64 17114 1 . ALA 65 65 17114 1 . VAL 66 66 17114 1 . ARG 67 67 17114 1 . LEU 68 68 17114 1 . ILE 69 69 17114 1 . LEU 70 70 17114 1 . PRO 71 71 17114 1 . GLY 72 72 17114 1 . GLU 73 73 17114 1 . LEU 74 74 17114 1 . ALA 75 75 17114 1 . LYS 76 76 17114 1 . HIS 77 77 17114 1 . ALA 78 78 17114 1 . VAL 79 79 17114 1 . SER 80 80 17114 1 . GLU 81 81 17114 1 . GLY 82 82 17114 1 . THR 83 83 17114 1 . ARG 84 84 17114 1 . ALA 85 85 17114 1 . VAL 86 86 17114 1 . THR 87 87 17114 1 . LYS 88 88 17114 1 . TYR 89 89 17114 1 . SER 90 90 17114 1 . SER 91 91 17114 1 . SER 92 92 17114 1 . THR 93 93 17114 1 . GLN 94 94 17114 1 . ALA 95 95 17114 1 . GLN 96 96 17114 1 . SER 97 97 17114 1 . SER 98 98 17114 1 . SER 99 99 17114 1 . ALA 100 100 17114 1 . ARG 101 101 17114 1 . ALA 102 102 17114 1 . GLY 103 103 17114 1 . LEU 104 104 17114 1 . GLN 105 105 17114 1 . PHE 106 106 17114 1 . PRO 107 107 17114 1 . VAL 108 108 17114 1 . GLY 109 109 17114 1 . ARG 110 110 17114 1 . ILE 111 111 17114 1 . LYS 112 112 17114 1 . ARG 113 113 17114 1 . TYR 114 114 17114 1 . LEU 115 115 17114 1 . LYS 116 116 17114 1 . ARG 117 117 17114 1 . HIS 118 118 17114 1 . ALA 119 119 17114 1 . THR 120 120 17114 1 . GLY 121 121 17114 1 . ARG 122 122 17114 1 . THR 123 123 17114 1 . ARG 124 124 17114 1 . VAL 125 125 17114 1 . GLY 126 126 17114 1 . SER 127 127 17114 1 . LYS 128 128 17114 1 . ALA 129 129 17114 1 . ALA 130 130 17114 1 . ILE 131 131 17114 1 . TYR 132 132 17114 1 . LEU 133 133 17114 1 . THR 134 134 17114 1 . ALA 135 135 17114 1 . VAL 136 136 17114 1 . LEU 137 137 17114 1 . GLU 138 138 17114 1 . TYR 139 139 17114 1 . LEU 140 140 17114 1 . THR 141 141 17114 1 . ALA 142 142 17114 1 . GLU 143 143 17114 1 . VAL 144 144 17114 1 . LEU 145 145 17114 1 . GLU 146 146 17114 1 . LEU 147 147 17114 1 . ALA 148 148 17114 1 . GLY 149 149 17114 1 . ASN 150 150 17114 1 . ALA 151 151 17114 1 . ALA 152 152 17114 1 . LYS 153 153 17114 1 . ASP 154 154 17114 1 . LEU 155 155 17114 1 . LYS 156 156 17114 1 . VAL 157 157 17114 1 . LYS 158 158 17114 1 . ARG 159 159 17114 1 . ILE 160 160 17114 1 . THR 161 161 17114 1 . PRO 162 162 17114 1 . ARG 163 163 17114 1 . HIS 164 164 17114 1 . LEU 165 165 17114 1 . GLN 166 166 17114 1 . LEU 167 167 17114 1 . ALA 168 168 17114 1 . ILE 169 169 17114 1 . ARG 170 170 17114 1 . GLY 171 171 17114 1 . ASP 172 172 17114 1 . ASP 173 173 17114 1 . GLU 174 174 17114 1 . LEU 175 175 17114 1 . ASP 176 176 17114 1 . SER 177 177 17114 1 . LEU 178 178 17114 1 . ILE 179 179 17114 1 . ARG 180 180 17114 1 . ALA 181 181 17114 1 . THR 182 182 17114 1 . ILE 183 183 17114 1 . ALA 184 184 17114 1 . SER 185 185 17114 1 . GLY 186 186 17114 1 . GLY 187 187 17114 1 . VAL 188 188 17114 1 . LEU 189 189 17114 1 . PRO 190 190 17114 1 . HIS 191 191 17114 1 . ILE 192 192 17114 1 stop_ save_ save_Chz1 _Entity.Sf_category entity _Entity.Sf_framecode Chz1 _Entity.Entry_ID 17114 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name Chz1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; TVEDSESDMDDAKLDALMGN EGEEEEDDLAEIDTSNIITS GRRTRGKVIDYKKTAEELDK KE ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 62 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-01-30 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 15393 . Chz1 . . . . . 100.00 62 100.00 100.00 1.25e-32 . . . . 17114 2 2 no PDB 2JSS . "Nmr Structure Of Chaperone Chz1 Complexed With Histone H2a.Z-H2b" . . . . . 100.00 62 100.00 100.00 1.25e-32 . . . . 17114 2 3 no DBJ GAA22856 . "K7_Chz1p [Saccharomyces cerevisiae Kyokai no. 7]" . . . . . 100.00 147 100.00 100.00 2.48e-32 . . . . 17114 2 4 no EMBL CAY79198 . "Chz1p [Saccharomyces cerevisiae EC1118]" . . . . . 100.00 160 100.00 100.00 2.61e-32 . . . . 17114 2 5 no GB AAB64563 . "Yer030wp [Saccharomyces cerevisiae]" . . . . . 100.00 160 100.00 100.00 4.47e-32 . . . . 17114 2 6 no GB AHY75583 . "Chz1p [Saccharomyces cerevisiae YJM993]" . . . . . 100.00 163 100.00 100.00 3.01e-32 . . . . 17114 2 7 no GB EDN62999 . "chaperone [Saccharomyces cerevisiae YJM789]" . . . . . 100.00 167 100.00 100.00 3.41e-32 . . . . 17114 2 8 no GB EDV08859 . "chaperone [Saccharomyces cerevisiae RM11-1a]" . . . . . 100.00 160 100.00 100.00 2.61e-32 . . . . 17114 2 9 no GB EDZ72631 . "YER030Wp-like protein [Saccharomyces cerevisiae AWRI1631]" . . . . . 100.00 170 100.00 100.00 3.10e-32 . . . . 17114 2 10 no REF NP_010947 . "Chz1p [Saccharomyces cerevisiae S288c]" . . . . . 100.00 153 100.00 100.00 3.00e-32 . . . . 17114 2 11 no SP A6ZQX9 . "RecName: Full=Histone H2A.Z-specific chaperone CHZ1 [Saccharomyces cerevisiae YJM789]" . . . . . 100.00 160 100.00 100.00 2.61e-32 . . . . 17114 2 12 no SP P40019 . "RecName: Full=Histone H2A.Z-specific chaperone CHZ1 [Saccharomyces cerevisiae S288c]" . . . . . 100.00 153 100.00 100.00 3.00e-32 . . . . 17114 2 13 no TPG DAA07683 . "TPA: Chz1p [Saccharomyces cerevisiae S288c]" . . . . . 100.00 153 100.00 100.00 3.00e-32 . . . . 17114 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . THR . 17114 2 2 . VAL . 17114 2 3 . GLU . 17114 2 4 . ASP . 17114 2 5 . SER . 17114 2 6 . GLU . 17114 2 7 . SER . 17114 2 8 . ASP . 17114 2 9 . MET . 17114 2 10 . ASP . 17114 2 11 . ASP . 17114 2 12 . ALA . 17114 2 13 . LYS . 17114 2 14 . LEU . 17114 2 15 . ASP . 17114 2 16 . ALA . 17114 2 17 . LEU . 17114 2 18 . MET . 17114 2 19 . GLY . 17114 2 20 . ASN . 17114 2 21 . GLU . 17114 2 22 . GLY . 17114 2 23 . GLU . 17114 2 24 . GLU . 17114 2 25 . GLU . 17114 2 26 . GLU . 17114 2 27 . ASP . 17114 2 28 . ASP . 17114 2 29 . LEU . 17114 2 30 . ALA . 17114 2 31 . GLU . 17114 2 32 . ILE . 17114 2 33 . ASP . 17114 2 34 . THR . 17114 2 35 . SER . 17114 2 36 . ASN . 17114 2 37 . ILE . 17114 2 38 . ILE . 17114 2 39 . THR . 17114 2 40 . SER . 17114 2 41 . GLY . 17114 2 42 . ARG . 17114 2 43 . ARG . 17114 2 44 . THR . 17114 2 45 . ARG . 17114 2 46 . GLY . 17114 2 47 . LYS . 17114 2 48 . VAL . 17114 2 49 . ILE . 17114 2 50 . ASP . 17114 2 51 . TYR . 17114 2 52 . LYS . 17114 2 53 . LYS . 17114 2 54 . THR . 17114 2 55 . ALA . 17114 2 56 . GLU . 17114 2 57 . GLU . 17114 2 58 . LEU . 17114 2 59 . ASP . 17114 2 60 . LYS . 17114 2 61 . LYS . 17114 2 62 . GLU . 17114 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . THR 1 1 17114 2 . VAL 2 2 17114 2 . GLU 3 3 17114 2 . ASP 4 4 17114 2 . SER 5 5 17114 2 . GLU 6 6 17114 2 . SER 7 7 17114 2 . ASP 8 8 17114 2 . MET 9 9 17114 2 . ASP 10 10 17114 2 . ASP 11 11 17114 2 . ALA 12 12 17114 2 . LYS 13 13 17114 2 . LEU 14 14 17114 2 . ASP 15 15 17114 2 . ALA 16 16 17114 2 . LEU 17 17 17114 2 . MET 18 18 17114 2 . GLY 19 19 17114 2 . ASN 20 20 17114 2 . GLU 21 21 17114 2 . GLY 22 22 17114 2 . GLU 23 23 17114 2 . GLU 24 24 17114 2 . GLU 25 25 17114 2 . GLU 26 26 17114 2 . ASP 27 27 17114 2 . ASP 28 28 17114 2 . LEU 29 29 17114 2 . ALA 30 30 17114 2 . GLU 31 31 17114 2 . ILE 32 32 17114 2 . ASP 33 33 17114 2 . THR 34 34 17114 2 . SER 35 35 17114 2 . ASN 36 36 17114 2 . ILE 37 37 17114 2 . ILE 38 38 17114 2 . THR 39 39 17114 2 . SER 40 40 17114 2 . GLY 41 41 17114 2 . ARG 42 42 17114 2 . ARG 43 43 17114 2 . THR 44 44 17114 2 . ARG 45 45 17114 2 . GLY 46 46 17114 2 . LYS 47 47 17114 2 . VAL 48 48 17114 2 . ILE 49 49 17114 2 . ASP 50 50 17114 2 . TYR 51 51 17114 2 . LYS 52 52 17114 2 . LYS 53 53 17114 2 . THR 54 54 17114 2 . ALA 55 55 17114 2 . GLU 56 56 17114 2 . GLU 57 57 17114 2 . LEU 58 58 17114 2 . ASP 59 59 17114 2 . LYS 60 60 17114 2 . LYS 61 61 17114 2 . GLU 62 62 17114 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 17114 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $H2AZH2B . 4932 organism . 'Saccharomyces cerevisiae' 'Baker's yeast' . . Eukaryota Fungi Saccharomyces cerevisiae . . . . . . . . . . . . . . . . . . . . . 17114 1 2 2 $Chz1 . . 'not applicable' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17114 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 17114 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $H2AZH2B . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . . . . . . . . . . . . . . na . . . . . . 17114 1 2 2 $Chz1 . 'chemical synthesis' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17114 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 17114 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 H2AZH2B '[U-15N; U-2H]' . . 1 $H2AZH2B . . 0.95 . . mM . . . . 17114 1 2 Chz1 '[U-15N; U-2H]' . . 2 $Chz1 . . 1 . . mM . . . . 17114 1 3 Mes 'natural abundance' . . . . . . 25 . . mM . . . . 17114 1 4 'sodium chloride' 'natural abundance' . . . . . . 0.2 . . M . . . . 17114 1 5 EDTA 'natural abundance' . . . . . . 1 . . mM . . . . 17114 1 6 H2O 'natural abundance' . . . . . . 90 . . % . . . . 17114 1 7 D2O 'natural abundance' . . . . . . 10 . . % . . . . 17114 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 17114 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 6.0 . pH 17114 1 pressure ambient . atm 17114 1 temperature 308 . K 17114 1 stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Software.Sf_category software _Software.Sf_framecode NMRPipe _Software.Entry_ID 17114 _Software.ID 1 _Software.Name NMRPipe _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 17114 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 17114 1 stop_ save_ save_FuDA _Software.Sf_category software _Software.Sf_framecode FuDA _Software.Entry_ID 17114 _Software.ID 2 _Software.Name FuDA _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'flemming@pound.med.utoronto.ca; http://pound.med.utoronto.ca/software' . . 17114 2 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'signal quantification' 17114 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 17114 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model INOVA _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_spectrometer_2 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_2 _NMR_spectrometer.Entry_ID 17114 _NMR_spectrometer.ID 2 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model INOVA _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 800 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 17114 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Varian INOVA . 600 . . . 17114 1 2 spectrometer_2 Varian INOVA . 800 . . . 17114 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 17114 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 17114 1 stop_ save_ save_Chemical_rate_list _Chemical_rate_list.Sf_category chemical_rates _Chemical_rate_list.Sf_framecode Chemical_rate_list _Chemical_rate_list.Entry_ID 17114 _Chemical_rate_list.ID 1 _Chemical_rate_list.Sample_condition_list_ID 1 _Chemical_rate_list.Sample_condition_list_label $sample_conditions_1 _Chemical_rate_list.Details . _Chemical_rate_list.Text_data_format . _Chemical_rate_list.Text_data . loop_ _Chemical_rate_experiment.Experiment_ID _Chemical_rate_experiment.Experiment_name _Chemical_rate_experiment.Sample_ID _Chemical_rate_experiment.Sample_label _Chemical_rate_experiment.Sample_state _Chemical_rate_experiment.Entry_ID _Chemical_rate_experiment.Chemical_rate_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 17114 1 stop_ loop_ _Chemical_rate.ID _Chemical_rate.Assembly_ID _Chemical_rate.Assembly_atom_ID _Chemical_rate.Entity_assembly_ID _Chemical_rate.Entity_ID _Chemical_rate.Comp_index_ID _Chemical_rate.Seq_ID _Chemical_rate.Comp_ID _Chemical_rate.Atom_ID _Chemical_rate.Atom_type _Chemical_rate.Atom_isotope_number _Chemical_rate.Val_type _Chemical_rate.Val _Chemical_rate.Val_min _Chemical_rate.Val_max _Chemical_rate.Val_err _Chemical_rate.Val_units _Chemical_rate.Resonance_ID _Chemical_rate.Auth_entity_assembly_ID _Chemical_rate.Auth_seq_ID _Chemical_rate.Auth_comp_ID _Chemical_rate.Auth_atom_ID _Chemical_rate.Entry_ID _Chemical_rate.Chemical_rate_list_ID 1 1 . 1 1 . . . . . . kex 1520 . . 165 s-1 . . . . . 117114 1 2 2 . 2 2 . . . . . . kex 1490 . . 100 s-1 . . . . . 117114 1 3 1 . 1 1 . . . . . . koff 22 . . 2 s-1 . . . . . 117114 1 stop_ save_ save_binding_data _Binding_value_list.Sf_category binding_data _Binding_value_list.Sf_framecode binding_data _Binding_value_list.Entry_ID 17114 _Binding_value_list.ID 1 _Binding_value_list.Sample_condition_list_ID 1 _Binding_value_list.Sample_condition_list_label $sample_conditions_1 _Binding_value_list.Details . _Binding_value_list.Text_data_format . _Binding_value_list.Text_data . loop_ _Binding_experiment.Experiment_ID _Binding_experiment.Experiment_name _Binding_experiment.Sample_ID _Binding_experiment.Sample_label _Binding_experiment.Sample_state _Binding_experiment.Entry_ID _Binding_experiment.Binding_value_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 17114 1 stop_ loop_ _Binding_software.Software_ID _Binding_software.Software_label _Binding_software.Method_ID _Binding_software.Method_label _Binding_software.Entry_ID _Binding_software.Binding_value_list_ID 1 $NMRPipe . . 17114 1 2 $FuDA . . 17114 1 stop_ loop_ _Binding_result.ID _Binding_result.Experiment_ID _Binding_result.Assembly_ID _Binding_result.Atm_obs_assembly_atom_ID _Binding_result.Atm_obs_entity_assembly_ID _Binding_result.Atm_obs_entity_ID _Binding_result.Atm_obs_comp_index_ID _Binding_result.Atm_obs_seq_ID _Binding_result.Atm_obs_comp_ID _Binding_result.Atm_obs_atom_ID _Binding_result.Atm_obs_atom_type _Binding_result.Atm_obs_atom_isotope_number _Binding_result.Resonance_ID _Binding_result.Atm_obs_auth_entity_assembly_ID _Binding_result.Atm_obs_auth_seq_ID _Binding_result.Atm_obs_auth_comp_ID _Binding_result.Atm_obs_auth_atom_ID _Binding_result.Expt_observed_param _Binding_result.Val_type _Binding_result.Val _Binding_result.Val_err _Binding_result.Val_units _Binding_result.Entry_ID _Binding_result.Binding_value_list_ID 1 . 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kd 0.22 0.2 uM 17114 1 2 . 1 . 1 1 . . . . . . . . . . . 'chemcial shifts' kon 1 0.01 GM 17114 1 stop_ loop_ _Binding_partners.Binding_result_ID _Binding_partners.Assembly_ID _Binding_partners.Entity_assembly_ID _Binding_partners.Entity_assembly_name _Binding_partners.Entity_ID _Binding_partners.Entity_label _Binding_partners.Entry_ID _Binding_partners.Binding_value_list_ID 1 1 1 H2AZH2B 1 $H2AZH2B 17114 1 1 1 2 Chz1 2 $Chz1 17114 1 2 2 1 H2AZH2B 1 $H2AZH2B 17114 1 2 2 2 Chz1 2 $Chz1 17114 1 stop_ save_