data_17225 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 17225 _Entry.Title ; The Solution Structure and DNA-binding Properties of the Cold-shock Domain of the Human Y-box Protein YB-1 ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2010-09-29 _Entry.Accession_date 2010-09-29 _Entry.Last_release_date 2010-11-10 _Entry.Original_release_date 2010-11-10 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Cathelijne Kloks . P.A.M. . 17225 2 Christian Spronk . A.E.M. . 17225 3 Edwin Lasonder . . . 17225 4 Astrid Hoffmann . . . 17225 5 Geerten Vuister . W. . 17225 6 Stephan Grzesiek . . . 17225 7 Cornelis Hilbers . W. . 17225 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID binding_constants 1 17225 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'binding constants' 2 17225 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2010-11-10 2010-09-29 original author . 17225 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 17225 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 11851341 _Citation.Full_citation . _Citation.Title 'The Solution Structure and DNA-binding Properties of the Cold-shock Domain of the Human Y-box Protein YB-1' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'J. Mol. Biol.' _Citation.Journal_name_full 'Journal of Molecular Biology' _Citation.Journal_volume 316 _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 317 _Citation.Page_last 326 _Citation.Year 2002 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Cathelijne Kloks . P.A.M. . 17225 1 2 Christian Spronk . A.E.M. . 17225 1 3 Edwin Lasonder . . . 17225 1 4 Astrid Hoffmann . . . 17225 1 5 Geerten Vuister . W. . 17225 1 6 Stephan Grzesiek . . . 17225 1 7 Cornelis Hilbers . W. . 17225 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 17225 _Assembly.ID 1 _Assembly.Name 'CSD/ssDNA complex' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 CSD 1 $CSD A . yes native no no . . . 17225 1 2 ssDNA 2 $ssDNA B . yes native no no . . . 17225 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_CSD _Entity.Sf_category entity _Entity.Sf_framecode CSD _Entity.Entry_ID 17225 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name CSD _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MKKVIATKVLGTVKWFNVRN GYGFINRNDTKEDVFVHQTA IKKNNPRKYLRSVGDGETVE FDVVEGEKGAEAANVTGPG ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 79 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 4147 . "Cold Shock domain" . . . . . 100.00 79 100.00 100.00 2.42e-48 . . . . 17225 1 2 no PDB 1H95 . "Solution Structure Of The Single-Stranded Dna-Binding Cold Shock Domain (Csd) Of Human Y-Box Protein 1 (Yb1) Determined By Nmr " . . . . . 100.00 79 100.00 100.00 2.42e-48 . . . . 17225 1 3 no DBJ BAA02569 . "DNA binding protein B [Rattus sp.]" . . . . . 98.73 322 100.00 100.00 4.99e-47 . . . . 17225 1 4 no DBJ BAA03376 . "dbpA murine homologue [Mus musculus domesticus]" . . . . . 97.47 308 98.70 100.00 9.60e-43 . . . . 17225 1 5 no DBJ BAA05380 . "unnamed protein product [Gallus gallus]" . . . . . 98.73 326 100.00 100.00 7.61e-47 . . . . 17225 1 6 no DBJ BAA19849 . "Y box protein 1 [Carassius auratus]" . . . . . 98.73 311 100.00 100.00 6.58e-47 . . . . 17225 1 7 no DBJ BAC45236 . "Y-box binding protein [Oryzias latipes]" . . . . . 98.73 306 100.00 100.00 7.97e-47 . . . . 17225 1 8 no EMBL CAA40847 . "Y box-binbing protein [Mus musculus]" . . . . . 98.73 321 100.00 100.00 4.33e-47 . . . . 17225 1 9 no EMBL CAA42778 . "YB3 [Xenopus laevis]" . . . . . 98.73 305 100.00 100.00 7.30e-47 . . . . 17225 1 10 no EMBL CAA51261 . "cold shock domain protein A [Homo sapiens]" . . . . . 97.47 372 98.70 100.00 6.11e-46 . . . . 17225 1 11 no EMBL CAA64631 . "DNA-binding protein [Homo sapiens]" . . . . . 97.47 372 98.70 100.00 6.11e-46 . . . . 17225 1 12 no EMBL CAB46826 . "DNA binding protein [Canis lupus familiaris]" . . . . . 78.48 96 98.39 100.00 1.31e-34 . . . . 17225 1 13 no GB AAA02573 . "YB-1 protein [Gallus gallus]" . . . . . 98.73 321 100.00 100.00 5.72e-47 . . . . 17225 1 14 no GB AAA20871 . "similar to dbpB protein [Homo sapiens]" . . . . . 98.73 324 100.00 100.00 5.63e-47 . . . . 17225 1 15 no GB AAA21677 . "transcription factor EF1(A) [Bos taurus]" . . . . . 72.15 110 100.00 100.00 6.23e-33 . . . . 17225 1 16 no GB AAA30497 . "transcription factor EF1(A) [Bos taurus]" . . . . . 98.73 324 100.00 100.00 5.63e-47 . . . . 17225 1 17 no GB AAA35749 . "DNA-binding protein A, partial [Homo sapiens]" . . . . . 97.47 412 98.70 100.00 1.26e-42 . . . . 17225 1 18 no PRF 2210343A . "nicotinic acetylcholine receptor repressor" . . . . . 97.47 361 98.70 100.00 7.54e-46 . . . . 17225 1 19 no REF NP_001003127 . "Y-box-binding protein 3 [Canis lupus familiaris]" . . . . . 97.47 304 97.40 98.70 4.73e-46 . . . . 17225 1 20 no REF NP_001016677 . "nuclease-sensitive element-binding protein 1 [Xenopus (Silurana) tropicalis]" . . . . . 98.73 306 100.00 100.00 8.23e-47 . . . . 17225 1 21 no REF NP_001076254 . "nuclease-sensitive element-binding protein 1 [Oryctolagus cuniculus]" . . . . . 98.73 324 100.00 100.00 5.39e-47 . . . . 17225 1 22 no REF NP_001079367 . "nuclease-sensitive element-binding protein 1 [Xenopus laevis]" . . . . . 97.47 303 100.00 100.00 3.21e-46 . . . . 17225 1 23 no REF NP_001080330 . "B box-binding protein [Xenopus laevis]" . . . . . 98.73 305 100.00 100.00 7.30e-47 . . . . 17225 1 24 no SP P16989 . "RecName: Full=Y-box-binding protein 3; AltName: Full=Cold shock domain-containing protein A; AltName: Full=DNA-binding protein " . . . . . 97.47 372 98.70 100.00 6.11e-46 . . . . 17225 1 25 no SP P21573 . "RecName: Full=Nuclease-sensitive element-binding protein 1; AltName: Full=Y-box transcription factor; AltName: Full=Y-box-bindi" . . . . . 97.47 303 100.00 100.00 3.01e-46 . . . . 17225 1 26 no SP P62960 . "RecName: Full=Nuclease-sensitive element-binding protein 1; AltName: Full=CCAAT-binding transcription factor I subunit A; Short" . . . . . 98.73 322 100.00 100.00 4.99e-47 . . . . 17225 1 27 no SP P62961 . "RecName: Full=Nuclease-sensitive element-binding protein 1; AltName: Full=CCAAT-binding transcription factor I subunit A; Short" . . . . . 98.73 322 100.00 100.00 4.99e-47 . . . . 17225 1 28 no SP P67808 . "RecName: Full=Nuclease-sensitive element-binding protein 1; AltName: Full=CCAAT-binding transcription factor I subunit A; Short" . . . . . 98.73 324 100.00 100.00 5.63e-47 . . . . 17225 1 29 no TPG DAA29365 . "TPA: cold shock domain protein A [Bos taurus]" . . . . . 97.47 376 98.70 100.00 1.35e-42 . . . . 17225 1 30 no TPG DAA30957 . "TPA: nuclease-sensitive element-binding protein 1 [Bos taurus]" . . . . . 98.73 324 100.00 100.00 5.63e-47 . . . . 17225 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 17225 1 2 . LYS . 17225 1 3 . LYS . 17225 1 4 . VAL . 17225 1 5 . ILE . 17225 1 6 . ALA . 17225 1 7 . THR . 17225 1 8 . LYS . 17225 1 9 . VAL . 17225 1 10 . LEU . 17225 1 11 . GLY . 17225 1 12 . THR . 17225 1 13 . VAL . 17225 1 14 . LYS . 17225 1 15 . TRP . 17225 1 16 . PHE . 17225 1 17 . ASN . 17225 1 18 . VAL . 17225 1 19 . ARG . 17225 1 20 . ASN . 17225 1 21 . GLY . 17225 1 22 . TYR . 17225 1 23 . GLY . 17225 1 24 . PHE . 17225 1 25 . ILE . 17225 1 26 . ASN . 17225 1 27 . ARG . 17225 1 28 . ASN . 17225 1 29 . ASP . 17225 1 30 . THR . 17225 1 31 . LYS . 17225 1 32 . GLU . 17225 1 33 . ASP . 17225 1 34 . VAL . 17225 1 35 . PHE . 17225 1 36 . VAL . 17225 1 37 . HIS . 17225 1 38 . GLN . 17225 1 39 . THR . 17225 1 40 . ALA . 17225 1 41 . ILE . 17225 1 42 . LYS . 17225 1 43 . LYS . 17225 1 44 . ASN . 17225 1 45 . ASN . 17225 1 46 . PRO . 17225 1 47 . ARG . 17225 1 48 . LYS . 17225 1 49 . TYR . 17225 1 50 . LEU . 17225 1 51 . ARG . 17225 1 52 . SER . 17225 1 53 . VAL . 17225 1 54 . GLY . 17225 1 55 . ASP . 17225 1 56 . GLY . 17225 1 57 . GLU . 17225 1 58 . THR . 17225 1 59 . VAL . 17225 1 60 . GLU . 17225 1 61 . PHE . 17225 1 62 . ASP . 17225 1 63 . VAL . 17225 1 64 . VAL . 17225 1 65 . GLU . 17225 1 66 . GLY . 17225 1 67 . GLU . 17225 1 68 . LYS . 17225 1 69 . GLY . 17225 1 70 . ALA . 17225 1 71 . GLU . 17225 1 72 . ALA . 17225 1 73 . ALA . 17225 1 74 . ASN . 17225 1 75 . VAL . 17225 1 76 . THR . 17225 1 77 . GLY . 17225 1 78 . PRO . 17225 1 79 . GLY . 17225 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 17225 1 . LYS 2 2 17225 1 . LYS 3 3 17225 1 . VAL 4 4 17225 1 . ILE 5 5 17225 1 . ALA 6 6 17225 1 . THR 7 7 17225 1 . LYS 8 8 17225 1 . VAL 9 9 17225 1 . LEU 10 10 17225 1 . GLY 11 11 17225 1 . THR 12 12 17225 1 . VAL 13 13 17225 1 . LYS 14 14 17225 1 . TRP 15 15 17225 1 . PHE 16 16 17225 1 . ASN 17 17 17225 1 . VAL 18 18 17225 1 . ARG 19 19 17225 1 . ASN 20 20 17225 1 . GLY 21 21 17225 1 . TYR 22 22 17225 1 . GLY 23 23 17225 1 . PHE 24 24 17225 1 . ILE 25 25 17225 1 . ASN 26 26 17225 1 . ARG 27 27 17225 1 . ASN 28 28 17225 1 . ASP 29 29 17225 1 . THR 30 30 17225 1 . LYS 31 31 17225 1 . GLU 32 32 17225 1 . ASP 33 33 17225 1 . VAL 34 34 17225 1 . PHE 35 35 17225 1 . VAL 36 36 17225 1 . HIS 37 37 17225 1 . GLN 38 38 17225 1 . THR 39 39 17225 1 . ALA 40 40 17225 1 . ILE 41 41 17225 1 . LYS 42 42 17225 1 . LYS 43 43 17225 1 . ASN 44 44 17225 1 . ASN 45 45 17225 1 . PRO 46 46 17225 1 . ARG 47 47 17225 1 . LYS 48 48 17225 1 . TYR 49 49 17225 1 . LEU 50 50 17225 1 . ARG 51 51 17225 1 . SER 52 52 17225 1 . VAL 53 53 17225 1 . GLY 54 54 17225 1 . ASP 55 55 17225 1 . GLY 56 56 17225 1 . GLU 57 57 17225 1 . THR 58 58 17225 1 . VAL 59 59 17225 1 . GLU 60 60 17225 1 . PHE 61 61 17225 1 . ASP 62 62 17225 1 . VAL 63 63 17225 1 . VAL 64 64 17225 1 . GLU 65 65 17225 1 . GLY 66 66 17225 1 . GLU 67 67 17225 1 . LYS 68 68 17225 1 . GLY 69 69 17225 1 . ALA 70 70 17225 1 . GLU 71 71 17225 1 . ALA 72 72 17225 1 . ALA 73 73 17225 1 . ASN 74 74 17225 1 . VAL 75 75 17225 1 . THR 76 76 17225 1 . GLY 77 77 17225 1 . PRO 78 78 17225 1 . GLY 79 79 17225 1 stop_ save_ save_ssDNA _Entity.Sf_category entity _Entity.Sf_framecode ssDNA _Entity.Entry_ID 17225 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name ssDNA _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polydeoxyribonucleotide _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ATTGG _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 5 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . DA . 17225 2 2 . DT . 17225 2 3 . DT . 17225 2 4 . DG . 17225 2 5 . DG . 17225 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . DA 1 1 17225 2 . DT 2 2 17225 2 . DT 3 3 17225 2 . DG 4 4 17225 2 . DG 5 5 17225 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 17225 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $CSD . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . . . . . . . . . 17225 1 2 2 $ssDNA . . 'no natural source' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17225 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 17225 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $CSD . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . . . . . . . . . . . . . . na . . . . . . 17225 1 2 2 $ssDNA . 'chemical synthesis' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17225 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 17225 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 4 _Sample.Solvent_system '95% H2O/5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 CSD 'natural abundance' . . 1 $CSD . . 2.7 . . mg . . . . 17225 1 2 ssDNA 'natural abundance' . . 2 $ssDNA . . . 0 2 equivalents . . . . 17225 1 3 H2O 'natural abundance' . . . . . . 95 . . % . . . . 17225 1 4 D2O 'natural abundance' . . . . . . 5 . . % . . . . 17225 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 17225 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 6.7 . pH 17225 1 pressure ambient . atm 17225 1 temperature 298 . K 17225 1 stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Software.Sf_category software _Software.Sf_framecode NMRPipe _Software.Entry_ID 17225 _Software.ID 1 _Software.Name NMRPipe _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 17225 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 17225 1 stop_ save_ save_PIPP _Software.Sf_category software _Software.Sf_framecode PIPP _Software.Entry_ID 17225 _Software.ID 2 _Software.Name PIPP _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Garrett, D. S., Powers, R., Gronenborn, A. M. & Clore, G. M.' . . 17225 2 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'peak picking' 17225 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 17225 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DRX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 500 save_ save_spectrometer_2 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_2 _NMR_spectrometer.Entry_ID 17225 _NMR_spectrometer.ID 2 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model UnityInova _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 750 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 17225 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker DRX . 500 . . . 17225 1 2 spectrometer_2 Varian UnityInova . 750 . . . 17225 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 17225 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 17225 1 stop_ save_ save_binding_data _Binding_value_list.Sf_category binding_data _Binding_value_list.Sf_framecode binding_data _Binding_value_list.Entry_ID 17225 _Binding_value_list.ID 1 _Binding_value_list.Sample_condition_list_ID 1 _Binding_value_list.Sample_condition_list_label $sample_conditions_1 _Binding_value_list.Details . _Binding_value_list.Text_data_format . _Binding_value_list.Text_data . loop_ _Binding_experiment.Experiment_ID _Binding_experiment.Experiment_name _Binding_experiment.Sample_ID _Binding_experiment.Sample_label _Binding_experiment.Sample_state _Binding_experiment.Entry_ID _Binding_experiment.Binding_value_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 17225 1 stop_ loop_ _Binding_software.Software_ID _Binding_software.Software_label _Binding_software.Method_ID _Binding_software.Method_label _Binding_software.Entry_ID _Binding_software.Binding_value_list_ID 1 $NMRPipe . . 17225 1 2 $PIPP . . 17225 1 stop_ loop_ _Binding_result.ID _Binding_result.Experiment_ID _Binding_result.Assembly_ID _Binding_result.Atm_obs_assembly_atom_ID _Binding_result.Atm_obs_entity_assembly_ID _Binding_result.Atm_obs_entity_ID _Binding_result.Atm_obs_comp_index_ID _Binding_result.Atm_obs_seq_ID _Binding_result.Atm_obs_comp_ID _Binding_result.Atm_obs_atom_ID _Binding_result.Atm_obs_atom_type _Binding_result.Atm_obs_atom_isotope_number _Binding_result.Resonance_ID _Binding_result.Atm_obs_auth_entity_assembly_ID _Binding_result.Atm_obs_auth_seq_ID _Binding_result.Atm_obs_auth_comp_ID _Binding_result.Atm_obs_auth_atom_ID _Binding_result.Expt_observed_param _Binding_result.Val_type _Binding_result.Val _Binding_result.Val_err _Binding_result.Val_units _Binding_result.Entry_ID _Binding_result.Binding_value_list_ID 1 1 1 . 1 1 . 15 TRP . . . . . . . . 'chemical shifts' Kd 0.00014 . M 17225 1 2 1 2 . 1 1 . 37 HIS . . . . . . . . 'chemical shifts' Kd 0.00016 . M 17225 1 stop_ loop_ _Binding_partners.Binding_result_ID _Binding_partners.Assembly_ID _Binding_partners.Entity_assembly_ID _Binding_partners.Entity_assembly_name _Binding_partners.Entity_ID _Binding_partners.Entity_label _Binding_partners.Entry_ID _Binding_partners.Binding_value_list_ID 1 1 1 CSD 1 $CSD 17225 1 1 1 2 YB 2 $YB 17225 1 2 1 1 CSD 1 $CSD 17225 1 2 1 2 YB 2 $YB 17225 1 stop_ save_