data_27333 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 27333 _Entry.Title ; Ataxin7N10Q (Residues:1-62) ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2017-12-07 _Entry.Accession_date 2017-12-07 _Entry.Last_release_date 2017-12-07 _Entry.Original_release_date 2017-12-07 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.0.16 _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Hongyu Hu . . Sr. . 27333 2 Junye Hong . . . . 27333 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 27333 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 82 27333 '15N chemical shifts' 39 27333 '1H chemical shifts' 69 27333 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2018-02-07 . original BMRB . 27333 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 27334 'Ataxin7N10QT3N9 (Residues:1-62)' 27333 BMRB 27335 'Ataxin7N22QT3N9 (Residues:1-62)' 27333 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 27333 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID . _Citation.Full_citation . _Citation.Title ; Structural and dynamic studies reveal that the alanine-rich region in the N-terminus of ataxin-7 initiates -helix formation of the polyglutamine tract but inhibits its amyloid aggregation ; _Citation.Status 'in preparation' _Citation.Type journal _Citation.Journal_abbrev 'J. Biol. Chem.' _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Hongyu Hong . . . . 27333 1 2 Junye Hong . . . . 27333 1 3 Dongdong Wang . . . . 27333 1 4 Wei Xue . . . . 27333 1 5 Hongwei Yue . . . . 27333 1 6 Hui Yang . . . . 27333 1 7 Leilei Jiang . . . . 27333 1 8 Wenning Wang . . . . 27333 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 27333 _Assembly.ID 1 _Assembly.Name Ataxin7N10Q _Assembly.BMRB_code . _Assembly.Number_of_components . _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 Ataxin7N10Q 1 $Atx7N10Q A . yes native no no . . . 27333 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_Atx7N10Q _Entity.Sf_category entity _Entity.Sf_framecode Atx7N10Q _Entity.Entry_ID 27333 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name Atx7N10Q _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MSERAADDVRGEPRRAAAAA GGAAAAAARQQQQQQQQQQP PPPQPQRQQHPPPPPRRTRP ED ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states yes _Entity.Ambiguous_chem_comp_sites yes _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 62 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 27333 1 2 . SER . 27333 1 3 . GLU . 27333 1 4 . ARG . 27333 1 5 . ALA . 27333 1 6 . ALA . 27333 1 7 . ASP . 27333 1 8 . ASP . 27333 1 9 . VAL . 27333 1 10 . ARG . 27333 1 11 . GLY . 27333 1 12 . GLU . 27333 1 13 . PRO . 27333 1 14 . ARG . 27333 1 15 . ARG . 27333 1 16 . ALA . 27333 1 17 . ALA . 27333 1 18 . ALA . 27333 1 19 . ALA . 27333 1 20 . ALA . 27333 1 21 . GLY . 27333 1 22 . GLY . 27333 1 23 . ALA . 27333 1 24 . ALA . 27333 1 25 . ALA . 27333 1 26 . ALA . 27333 1 27 . ALA . 27333 1 28 . ALA . 27333 1 29 . ARG . 27333 1 30 . GLN . 27333 1 31 . GLN . 27333 1 32 . GLN . 27333 1 33 . GLN . 27333 1 34 . GLN . 27333 1 35 . GLN . 27333 1 36 . GLN . 27333 1 37 . GLN . 27333 1 38 . GLN . 27333 1 39 . GLN . 27333 1 40 . PRO . 27333 1 41 . PRO . 27333 1 42 . PRO . 27333 1 43 . PRO . 27333 1 44 . GLN . 27333 1 45 . PRO . 27333 1 46 . GLN . 27333 1 47 . ARG . 27333 1 48 . GLN . 27333 1 49 . GLN . 27333 1 50 . HIS . 27333 1 51 . PRO . 27333 1 52 . PRO . 27333 1 53 . PRO . 27333 1 54 . PRO . 27333 1 55 . PRO . 27333 1 56 . ARG . 27333 1 57 . ARG . 27333 1 58 . THR . 27333 1 59 . ARG . 27333 1 60 . PRO . 27333 1 61 . GLU . 27333 1 62 . ASP . 27333 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 27333 1 . SER 2 2 27333 1 . GLU 3 3 27333 1 . ARG 4 4 27333 1 . ALA 5 5 27333 1 . ALA 6 6 27333 1 . ASP 7 7 27333 1 . ASP 8 8 27333 1 . VAL 9 9 27333 1 . ARG 10 10 27333 1 . GLY 11 11 27333 1 . GLU 12 12 27333 1 . PRO 13 13 27333 1 . ARG 14 14 27333 1 . ARG 15 15 27333 1 . ALA 16 16 27333 1 . ALA 17 17 27333 1 . ALA 18 18 27333 1 . ALA 19 19 27333 1 . ALA 20 20 27333 1 . GLY 21 21 27333 1 . GLY 22 22 27333 1 . ALA 23 23 27333 1 . ALA 24 24 27333 1 . ALA 25 25 27333 1 . ALA 26 26 27333 1 . ALA 27 27 27333 1 . ALA 28 28 27333 1 . ARG 29 29 27333 1 . GLN 30 30 27333 1 . GLN 31 31 27333 1 . GLN 32 32 27333 1 . GLN 33 33 27333 1 . GLN 34 34 27333 1 . GLN 35 35 27333 1 . GLN 36 36 27333 1 . GLN 37 37 27333 1 . GLN 38 38 27333 1 . GLN 39 39 27333 1 . PRO 40 40 27333 1 . PRO 41 41 27333 1 . PRO 42 42 27333 1 . PRO 43 43 27333 1 . GLN 44 44 27333 1 . PRO 45 45 27333 1 . GLN 46 46 27333 1 . ARG 47 47 27333 1 . GLN 48 48 27333 1 . GLN 49 49 27333 1 . HIS 50 50 27333 1 . PRO 51 51 27333 1 . PRO 52 52 27333 1 . PRO 53 53 27333 1 . PRO 54 54 27333 1 . PRO 55 55 27333 1 . ARG 56 56 27333 1 . ARG 57 57 27333 1 . THR 58 58 27333 1 . ARG 59 59 27333 1 . PRO 60 60 27333 1 . GLU 61 61 27333 1 . ASP 62 62 27333 1 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 27333 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $Atx7N10Q . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 27333 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 27333 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $Atx7N10Q . 'recombinant technology' 'E. coli - cell free' . . . . . . . . . . . pET-32M . . . 27333 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 27333 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 Atx7N10Q '[U-13C; U-15N]' . . 1 $Atx7N10Q . . 300 100 500 uM . . . . 27333 1 2 Atx7N10Q [U-15N] . . 1 $Atx7N10Q . . 300 100 500 uM . . . . 27333 1 stop_ save_ ####################### # Sample conditions # ####################### save_NMRbuffer _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode NMRbuffer _Sample_condition_list.Entry_ID 27333 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 70 . mM 27333 1 pH 6.5 . pH 27333 1 pressure 1 . atm 27333 1 temperature 298 . K 27333 1 stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Software.Sf_category software _Software.Sf_framecode SPARKY _Software.Entry_ID 27333 _Software.ID 1 _Software.Type . _Software.Name SPARKY _Software.Version . _Software.DOI . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 27333 1 Goddard . . 27333 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' 27333 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 27333 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'Uniform NMR System' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 27333 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker 'Uniform NMR System' . 600 . . . 27333 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 27333 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $NMRbuffer . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 27333 1 2 '3D HNCACB' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $NMRbuffer . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 27333 1 3 '3D CBCA(CO)NH' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $NMRbuffer . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 27333 1 4 '3D HNHA' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $NMRbuffer . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 27333 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chemical_shift_reference_1 _Chem_shift_reference.Entry_ID 27333 _Chem_shift_reference.ID 1 _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 DSS 'methyl protons' . . . . ppm 0.00 na indirect 0.251449530 . . . . . 27333 1 H 1 DSS 'methyl protons' . . . . ppm 0.00 internal direct 1.000000000 . . . . . 27333 1 N 15 DSS 'methyl protons' . . . . ppm 0.00 na indirect 0.101329118 . . . . . 27333 1 P 31 DSS 'methyl protons' . . . . ppm 0.00 na indirect 0.404808636 . . . . . 27333 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chem_shift_list_1 _Assigned_chem_shift_list.Entry_ID 27333 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $NMRbuffer _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-15N HSQC' . . . 27333 1 2 '3D HNCACB' . . . 27333 1 3 '3D CBCA(CO)NH' . . . 27333 1 4 '3D HNHA' . . . 27333 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 1 1 1 MET H H 1 8.602 . . . . . . . . 1 M H . 27333 1 2 . 1 1 1 1 MET HA H 1 4.378 . . . . . . . . 1 M HA . 27333 1 3 . 1 1 1 1 MET CA C 13 55.763 . . . . . . . . 1 M CA . 27333 1 4 . 1 1 1 1 MET CB C 13 32.380 . . . . . . . . 1 M CB . 27333 1 5 . 1 1 1 1 MET N N 15 122.355 . . . . . . . . 1 M N . 27333 1 6 . 1 1 2 2 SER H H 1 8.253 . . . . . . . . 2 S H . 27333 1 7 . 1 1 2 2 SER CA C 13 58.541 . . . . . . . . 2 S CA . 27333 1 8 . 1 1 2 2 SER CB C 13 63.463 . . . . . . . . 2 S CB . 27333 1 9 . 1 1 2 2 SER N N 15 116.671 . . . . . . . . 2 S N . 27333 1 10 . 1 1 3 3 GLU H H 1 8.285 . . . . . . . . 3 E H . 27333 1 11 . 1 1 3 3 GLU CA C 13 56.506 . . . . . . . . 3 E CA . 27333 1 12 . 1 1 3 3 GLU CB C 13 30.086 . . . . . . . . 3 E CB . 27333 1 13 . 1 1 3 3 GLU N N 15 122.973 . . . . . . . . 3 E N . 27333 1 14 . 1 1 4 4 ARG H H 1 8.205 . . . . . . . . 4 R H . 27333 1 15 . 1 1 4 4 ARG HA H 1 4.147 . . . . . . . . 4 R HA . 27333 1 16 . 1 1 4 4 ARG CA C 13 55.919 . . . . . . . . 4 R CA . 27333 1 17 . 1 1 4 4 ARG CB C 13 30.695 . . . . . . . . 4 R CB . 27333 1 18 . 1 1 4 4 ARG N N 15 121.957 . . . . . . . . 4 R N . 27333 1 19 . 1 1 5 5 ALA H H 1 8.333 . . . . . . . . 5 A H . 27333 1 20 . 1 1 5 5 ALA HA H 1 4.100 . . . . . . . . 5 A HA . 27333 1 21 . 1 1 5 5 ALA CA C 13 52.358 . . . . . . . . 5 A CA . 27333 1 22 . 1 1 5 5 ALA CB C 13 18.970 . . . . . . . . 5 A CB . 27333 1 23 . 1 1 5 5 ALA N N 15 125.635 . . . . . . . . 5 A N . 27333 1 24 . 1 1 6 6 ALA H H 1 8.294 . . . . . . . . 6 A H . 27333 1 25 . 1 1 6 6 ALA HA H 1 4.129 . . . . . . . . 6 A HA . 27333 1 26 . 1 1 6 6 ALA CA C 13 52.716 . . . . . . . . 6 A CA . 27333 1 27 . 1 1 6 6 ALA CB C 13 18.952 . . . . . . . . 6 A CB . 27333 1 28 . 1 1 6 6 ALA N N 15 123.410 . . . . . . . . 6 A N . 27333 1 29 . 1 1 7 7 ASP H H 1 8.153 . . . . . . . . 7 D H . 27333 1 30 . 1 1 7 7 ASP HA H 1 4.391 . . . . . . . . 7 D HA . 27333 1 31 . 1 1 7 7 ASP CA C 13 54.286 . . . . . . . . 7 D CA . 27333 1 32 . 1 1 7 7 ASP CB C 13 40.765 . . . . . . . . 7 D CB . 27333 1 33 . 1 1 7 7 ASP N N 15 118.465 . . . . . . . . 7 D N . 27333 1 34 . 1 1 8 8 ASP H H 1 8.049 . . . . . . . . 8 D H . 27333 1 35 . 1 1 8 8 ASP HA H 1 4.446 . . . . . . . . 8 D HA . 27333 1 36 . 1 1 8 8 ASP CA C 13 54.197 . . . . . . . . 8 D CA . 27333 1 37 . 1 1 8 8 ASP CB C 13 40.897 . . . . . . . . 8 D CB . 27333 1 38 . 1 1 8 8 ASP N N 15 120.438 . . . . . . . . 8 D N . 27333 1 39 . 1 1 9 9 VAL H H 1 7.997 . . . . . . . . 9 V H . 27333 1 40 . 1 1 9 9 VAL HA H 1 3.936 . . . . . . . . 9 V HA . 27333 1 41 . 1 1 9 9 VAL CA C 13 62.573 . . . . . . . . 9 V CA . 27333 1 42 . 1 1 9 9 VAL CB C 13 32.071 . . . . . . . . 9 V CB . 27333 1 43 . 1 1 9 9 VAL N N 15 120.568 . . . . . . . . 9 V N . 27333 1 44 . 1 1 10 10 ARG H H 1 8.311 . . . . . . . . 10 R H . 27333 1 45 . 1 1 10 10 ARG CA C 13 56.296 . . . . . . . . 10 R CA . 27333 1 46 . 1 1 10 10 ARG CB C 13 30.446 . . . . . . . . 10 R CB . 27333 1 47 . 1 1 10 10 ARG N N 15 123.907 . . . . . . . . 10 R N . 27333 1 48 . 1 1 11 11 GLY H H 1 8.232 . . . . . . . . 11 G H . 27333 1 49 . 1 1 11 11 GLY HA2 H 1 3.789 . . . . . . . . 11 G HA . 27333 1 50 . 1 1 11 11 GLY HA3 H 1 3.789 . . . . . . . . 11 G HA . 27333 1 51 . 1 1 11 11 GLY CA C 13 44.874 . . . . . . . . 11 G CA . 27333 1 52 . 1 1 11 11 GLY N N 15 109.200 . . . . . . . . 11 G N . 27333 1 53 . 1 1 12 12 GLU H H 1 8.129 . . . . . . . . 12 E H . 27333 1 54 . 1 1 12 12 GLU HA H 1 4.422 . . . . . . . . 12 E HA . 27333 1 55 . 1 1 12 12 GLU CA C 13 54.166 . . . . . . . . 12 E CA . 27333 1 56 . 1 1 12 12 GLU CB C 13 29.564 . . . . . . . . 12 E CB . 27333 1 57 . 1 1 12 12 GLU N N 15 121.798 . . . . . . . . 12 E N . 27333 1 58 . 1 1 13 13 PRO CA C 13 62.534 . . . . . . . . 13 P CA . 27333 1 59 . 1 1 13 13 PRO CB C 13 31.883 . . . . . . . . 13 P CB . 27333 1 60 . 1 1 14 14 ARG H H 1 8.351 . . . . . . . . 14 R H . 27333 1 61 . 1 1 14 14 ARG HA H 1 4.146 . . . . . . . . 14 R HA . 27333 1 62 . 1 1 14 14 ARG CA C 13 55.846 . . . . . . . . 14 R CA . 27333 1 63 . 1 1 14 14 ARG CB C 13 30.635 . . . . . . . . 14 R CB . 27333 1 64 . 1 1 14 14 ARG N N 15 121.680 . . . . . . . . 14 R N . 27333 1 65 . 1 1 15 15 ARG H H 1 8.418 . . . . . . . . 15 R H . 27333 1 66 . 1 1 15 15 ARG HA H 1 4.139 . . . . . . . . 15 R HA . 27333 1 67 . 1 1 15 15 ARG CA C 13 55.835 . . . . . . . . 15 R CA . 27333 1 68 . 1 1 15 15 ARG CB C 13 30.797 . . . . . . . . 15 R CB . 27333 1 69 . 1 1 15 15 ARG N N 15 121.992 . . . . . . . . 15 R N . 27333 1 70 . 1 1 16 16 ALA H H 1 8.370 . . . . . . . . 16 A H . 27333 1 71 . 1 1 16 16 ALA HA H 1 4.124 . . . . . . . . 16 A HA . 27333 1 72 . 1 1 16 16 ALA CA C 13 52.356 . . . . . . . . 16 A CA . 27333 1 73 . 1 1 16 16 ALA CB C 13 19.005 . . . . . . . . 16 A CB . 27333 1 74 . 1 1 16 16 ALA N N 15 126.164 . . . . . . . . 16 A N . 27333 1 75 . 1 1 17 17 ALA H H 1 8.160 . . . . . . . . 17 A H . 27333 1 76 . 1 1 17 17 ALA HA H 1 4.090 . . . . . . . . 17 A HA . 27333 1 77 . 1 1 17 17 ALA CA C 13 52.322 . . . . . . . . 17 A CA . 27333 1 78 . 1 1 17 17 ALA CB C 13 18.918 . . . . . . . . 17 A CB . 27333 1 79 . 1 1 17 17 ALA N N 15 123.291 . . . . . . . . 17 A N . 27333 1 80 . 1 1 18 18 ALA H H 1 8.218 . . . . . . . . 18 A H . 27333 1 81 . 1 1 18 18 ALA HA H 1 4.096 . . . . . . . . 18 A HA . 27333 1 82 . 1 1 18 18 ALA CA C 13 52.335 . . . . . . . . 18 A CA . 27333 1 83 . 1 1 18 18 ALA CB C 13 18.904 . . . . . . . . 18 A CB . 27333 1 84 . 1 1 18 18 ALA N N 15 123.484 . . . . . . . . 18 A N . 27333 1 85 . 1 1 19 19 ALA H H 1 8.297 . . . . . . . . 19 A H . 27333 1 86 . 1 1 19 19 ALA HA H 1 4.119 . . . . . . . . 19 A HA . 27333 1 87 . 1 1 19 19 ALA CA C 13 52.794 . . . . . . . . 19 A CA . 27333 1 88 . 1 1 19 19 ALA CB C 13 18.984 . . . . . . . . 19 A CB . 27333 1 89 . 1 1 19 19 ALA N N 15 122.996 . . . . . . . . 19 A N . 27333 1 90 . 1 1 20 20 ALA CA C 13 52.542 . . . . . . . . 20 A CA . 27333 1 91 . 1 1 20 20 ALA CB C 13 18.896 . . . . . . . . 20 A CB . 27333 1 92 . 1 1 21 21 GLY H H 1 8.322 . . . . . . . . 21 G H . 27333 1 93 . 1 1 21 21 GLY HA2 H 1 3.833 . . . . . . . . 21 G HA . 27333 1 94 . 1 1 21 21 GLY HA3 H 1 3.833 . . . . . . . . 21 G HA . 27333 1 95 . 1 1 21 21 GLY CA C 13 45.195 . . . . . . . . 21 G CA . 27333 1 96 . 1 1 21 21 GLY N N 15 108.138 . . . . . . . . 21 G N . 27333 1 97 . 1 1 22 22 GLY H H 1 8.182 . . . . . . . . 22 G H . 27333 1 98 . 1 1 22 22 GLY CA C 13 45.173 . . . . . . . . 22 G CA . 27333 1 99 . 1 1 22 22 GLY N N 15 108.825 . . . . . . . . 22 G N . 27333 1 100 . 1 1 23 23 ALA H H 1 8.275 . . . . . . . . 23 A H . 27333 1 101 . 1 1 23 23 ALA HA H 1 4.095 . . . . . . . . 23 A HA . 27333 1 102 . 1 1 23 23 ALA CA C 13 53.817 . . . . . . . . 23 A CA . 27333 1 103 . 1 1 23 23 ALA CB C 13 19.136 . . . . . . . . 23 A CB . 27333 1 104 . 1 1 23 23 ALA N N 15 124.447 . . . . . . . . 23 A N . 27333 1 105 . 1 1 24 24 ALA CA C 13 53.364 . . . . . . . . 24 A CA . 27333 1 106 . 1 1 24 24 ALA CB C 13 18.354 . . . . . . . . 24 A CB . 27333 1 107 . 1 1 25 25 ALA H H 1 8.001 . . . . . . . . 25 A H . 27333 1 108 . 1 1 25 25 ALA HA H 1 4.065 . . . . . . . . 25 A HA . 27333 1 109 . 1 1 25 25 ALA CA C 13 53.406 . . . . . . . . 25 A CA . 27333 1 110 . 1 1 25 25 ALA CB C 13 18.444 . . . . . . . . 25 A CB . 27333 1 111 . 1 1 25 25 ALA N N 15 122.322 . . . . . . . . 25 A N . 27333 1 112 . 1 1 26 26 ALA H H 1 8.004 . . . . . . . . 26 A H . 27333 1 113 . 1 1 26 26 ALA HA H 1 4.067 . . . . . . . . 26 A HA . 27333 1 114 . 1 1 26 26 ALA CA C 13 53.491 . . . . . . . . 26 A CA . 27333 1 115 . 1 1 26 26 ALA CB C 13 18.570 . . . . . . . . 26 A CB . 27333 1 116 . 1 1 26 26 ALA N N 15 122.688 . . . . . . . . 26 A N . 27333 1 117 . 1 1 28 28 ALA H H 1 7.944 . . . . . . . . 28 A H . 27333 1 118 . 1 1 28 28 ALA HA H 1 4.089 . . . . . . . . 28 A HA . 27333 1 119 . 1 1 28 28 ALA CA C 13 53.391 . . . . . . . . 28 A CA . 27333 1 120 . 1 1 28 28 ALA CB C 13 18.486 . . . . . . . . 28 A CB . 27333 1 121 . 1 1 28 28 ALA N N 15 122.015 . . . . . . . . 28 A N . 27333 1 122 . 1 1 29 29 ARG H H 1 7.961 . . . . . . . . 29 R H . 27333 1 123 . 1 1 29 29 ARG HA H 1 4.077 . . . . . . . . 29 R HA . 27333 1 124 . 1 1 29 29 ARG CA C 13 57.105 . . . . . . . . 29 R CA . 27333 1 125 . 1 1 29 29 ARG CB C 13 30.243 . . . . . . . . 29 R CB . 27333 1 126 . 1 1 29 29 ARG N N 15 119.242 . . . . . . . . 29 R N . 27333 1 127 . 1 1 30 30 GLN H H 1 8.101 . . . . . . . . 30 Q H . 27333 1 128 . 1 1 30 30 GLN N N 15 120.347 . . . . . . . . 30 Q N . 27333 1 129 . 1 1 33 33 GLN H H 1 8.414 . . . . . . . . 33 Q H . 27333 1 130 . 1 1 33 33 GLN N N 15 121.898 . . . . . . . . 33 Q N . 27333 1 131 . 1 1 37 37 GLN H H 1 8.383 . . . . . . . . 37 Q H . 27333 1 132 . 1 1 37 37 GLN N N 15 121.227 . . . . . . . . 37 Q N . 27333 1 133 . 1 1 38 38 GLN H H 1 8.348 . . . . . . . . 38 Q H . 27333 1 134 . 1 1 38 38 GLN CA C 13 56.663 . . . . . . . . 38 Q CA . 27333 1 135 . 1 1 38 38 GLN CB C 13 28.786 . . . . . . . . 38 Q CB . 27333 1 136 . 1 1 38 38 GLN N N 15 121.104 . . . . . . . . 38 Q N . 27333 1 137 . 1 1 39 39 GLN H H 1 8.189 . . . . . . . . 39 Q H . 27333 1 138 . 1 1 39 39 GLN HA H 1 4.081 . . . . . . . . 39 Q HA . 27333 1 139 . 1 1 39 39 GLN CA C 13 56.745 . . . . . . . . 39 Q CA . 27333 1 140 . 1 1 39 39 GLN CB C 13 28.931 . . . . . . . . 39 Q CB . 27333 1 141 . 1 1 39 39 GLN N N 15 120.581 . . . . . . . . 39 Q N . 27333 1 142 . 1 1 43 43 PRO CA C 13 62.858 . . . . . . . . 43 P CA . 27333 1 143 . 1 1 43 43 PRO CB C 13 31.889 . . . . . . . . 43 P CB . 27333 1 144 . 1 1 44 44 GLN H H 1 8.399 . . . . . . . . 44 Q H . 27333 1 145 . 1 1 44 44 GLN CA C 13 55.951 . . . . . . . . 44 Q CA . 27333 1 146 . 1 1 44 44 GLN CB C 13 29.020 . . . . . . . . 44 Q CB . 27333 1 147 . 1 1 44 44 GLN N N 15 121.318 . . . . . . . . 44 Q N . 27333 1 148 . 1 1 45 45 PRO CA C 13 62.993 . . . . . . . . 45 P CA . 27333 1 149 . 1 1 45 45 PRO CB C 13 31.959 . . . . . . . . 45 P CB . 27333 1 150 . 1 1 46 46 GLN H H 1 8.497 . . . . . . . . 46 Q H . 27333 1 151 . 1 1 46 46 GLN HA H 1 4.127 . . . . . . . . 46 Q HA . 27333 1 152 . 1 1 46 46 GLN CA C 13 55.711 . . . . . . . . 46 Q CA . 27333 1 153 . 1 1 46 46 GLN CB C 13 29.281 . . . . . . . . 46 Q CB . 27333 1 154 . 1 1 46 46 GLN N N 15 120.942 . . . . . . . . 46 Q N . 27333 1 155 . 1 1 47 47 ARG H H 1 8.399 . . . . . . . . 47 R H . 27333 1 156 . 1 1 47 47 ARG HA H 1 4.151 . . . . . . . . 47 R HA . 27333 1 157 . 1 1 47 47 ARG CA C 13 55.848 . . . . . . . . 47 R CA . 27333 1 158 . 1 1 47 47 ARG CB C 13 30.785 . . . . . . . . 47 R CB . 27333 1 159 . 1 1 47 47 ARG N N 15 122.813 . . . . . . . . 47 R N . 27333 1 160 . 1 1 48 48 GLN H H 1 8.463 . . . . . . . . 48 Q H . 27333 1 161 . 1 1 48 48 GLN CA C 13 53.527 . . . . . . . . 48 Q CA . 27333 1 162 . 1 1 48 48 GLN CB C 13 28.828 . . . . . . . . 48 Q CB . 27333 1 163 . 1 1 48 48 GLN N N 15 123.416 . . . . . . . . 48 Q N . 27333 1 164 . 1 1 56 56 ARG CA C 13 55.820 . . . . . . . . 56 R CA . 27333 1 165 . 1 1 57 57 ARG H H 1 8.391 . . . . . . . . 57 R H . 27333 1 166 . 1 1 57 57 ARG CA C 13 55.777 . . . . . . . . 57 R CA . 27333 1 167 . 1 1 57 57 ARG CB C 13 30.768 . . . . . . . . 57 R CB . 27333 1 168 . 1 1 57 57 ARG N N 15 120.800 . . . . . . . . 57 R N . 27333 1 169 . 1 1 58 58 THR H H 1 8.283 . . . . . . . . 58 T H . 27333 1 170 . 1 1 58 58 THR HA H 1 4.153 . . . . . . . . 58 T HA . 27333 1 171 . 1 1 58 58 THR CA C 13 61.959 . . . . . . . . 58 T CA . 27333 1 172 . 1 1 58 58 THR CB C 13 69.675 . . . . . . . . 58 T CB . 27333 1 173 . 1 1 58 58 THR N N 15 116.273 . . . . . . . . 58 T N . 27333 1 174 . 1 1 59 59 ARG H H 1 8.270 . . . . . . . . 59 R H . 27333 1 175 . 1 1 59 59 ARG HA H 1 4.545 . . . . . . . . 59 R HA . 27333 1 176 . 1 1 59 59 ARG CA C 13 53.904 . . . . . . . . 59 R CA . 27333 1 177 . 1 1 59 59 ARG CB C 13 30.218 . . . . . . . . 59 R CB . 27333 1 178 . 1 1 59 59 ARG N N 15 123.941 . . . . . . . . 59 R N . 27333 1 179 . 1 1 60 60 PRO CA C 13 63.462 . . . . . . . . 60 P CA . 27333 1 180 . 1 1 60 60 PRO CB C 13 31.878 . . . . . . . . 60 P CB . 27333 1 181 . 1 1 61 61 GLU H H 1 8.520 . . . . . . . . 61 E H . 27333 1 182 . 1 1 61 61 GLU HA H 1 4.107 . . . . . . . . 61 E HA . 27333 1 183 . 1 1 61 61 GLU CA C 13 56.496 . . . . . . . . 61 E CA . 27333 1 184 . 1 1 61 61 GLU CB C 13 29.841 . . . . . . . . 61 E CB . 27333 1 185 . 1 1 61 61 GLU N N 15 119.758 . . . . . . . . 61 E N . 27333 1 186 . 1 1 62 62 ASP H H 1 7.789 . . . . . . . . 62 D H . 27333 1 187 . 1 1 62 62 ASP HA H 1 4.199 . . . . . . . . 62 D HA . 27333 1 188 . 1 1 62 62 ASP CA C 13 56.151 . . . . . . . . 62 D CA . 27333 1 189 . 1 1 62 62 ASP CB C 13 41.922 . . . . . . . . 62 D CB . 27333 1 190 . 1 1 62 62 ASP N N 15 126.772 . . . . . . . . 62 D N . 27333 1 stop_ save_