BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5666

Title: 1H, 15N and 13C backbone assignment of the Green Fluorescent Protein (GFP)

Authors: Khan, Farid; Stott, Katherine; Jackson, Sophie

Citation: Khan, Farid; Stott, Katherine; Jackson, Sophie. "Letter to the Editor: 1H, 15N and 13C backbone assignment of the Green Fluorescent Protein (GFP)"  J. Biomol. NMR 26, 281-282 (2003).

Assembly members:
Green Fluorescent Protein, polymer, 229 residues, Formula weight is not available

Natural source:   Common Name: jellyfish   Taxonomy ID: 6100   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aequorea victoria

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Green Fluorescent Protein: GSKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYG KLTLKFICTTGKLPVPWPTL VTTFSYGVQCFSRYPDHMKR HDFFKSAMPEGYVQERTISF KDDGNYKTRAEVKFEGDTLV NRIELKGIDFKEDGNILGHK LEYNYNSHNVYITADKQKNG IKANFKIRHNIEDGSVQLAD HYQQNTPIGDGPVLLPDNHY LSTQSALSKDPNEKRDHMVL LEFVTAAGI

Data sets:
Data typeCount
1H chemical shifts199
13C chemical shifts588
15N chemical shifts199

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GFP monomer1

Entities:

Entity 1, GFP monomer 229 residues - Formula weight is not available

1   GLYSERLYSGLYGLUGLULEUPHETHRGLY
2   VALVALPROILELEUVALGLULEUASPGLY
3   ASPVALASNGLYHISLYSPHESERVALSER
4   GLYGLUGLYGLUGLYASPALATHRTYRGLY
5   LYSLEUTHRLEULYSPHEILECYSTHRTHR
6   GLYLYSLEUPROVALPROTRPPROTHRLEU
7   VALTHRTHRPHESERTYRGLYVALGLNCYS
8   PHESERARGTYRPROASPHISMETLYSARG
9   HISASPPHEPHELYSSERALAMETPROGLU
10   GLYTYRVALGLNGLUARGTHRILESERPHE
11   LYSASPASPGLYASNTYRLYSTHRARGALA
12   GLUVALLYSPHEGLUGLYASPTHRLEUVAL
13   ASNARGILEGLULEULYSGLYILEASPPHE
14   LYSGLUASPGLYASNILELEUGLYHISLYS
15   LEUGLUTYRASNTYRASNSERHISASNVAL
16   TYRILETHRALAASPLYSGLNLYSASNGLY
17   ILELYSALAASNPHELYSILEARGHISASN
18   ILEGLUASPGLYSERVALGLNLEUALAASP
19   HISTYRGLNGLNASNTHRPROILEGLYASP
20   GLYPROVALLEULEUPROASPASNHISTYR
21   LEUSERTHRGLNSERALALEUSERLYSASP
22   PROASNGLULYSARGASPHISMETVALLEU
23   LEUGLUPHEVALTHRALAALAGLYILE

Samples:

sample_1: Green Fluorescent Protein, [U-95% 13C; U-95% 15N], 1.0 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 6.0 mM

sample_2: Green Fluorescent Protein, [U-95% 13C; U-95% 15N; U-75% 2H], 1.0 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 6.0 mM

Experimental_conditions: pH: 7.2 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

GenBank AAA69548 AAA69544 AAA69546 AAA27721 AAA69542
SWISS-PROT P42212
EMBL CAB70975 CAC27829 CAA65278 CAB61435 CAA10279
DBJ BAG30881 BAA93576 BAE45350
PDB
BMRB 5514